Estudios genéticos de la biosíntesis de Poli(3-Hidroxibutirato)PHB, en Bacillus megaterium. Obtención y caracterización de mutantes y expresión heteróloga de genes phb

Autores
Floccari, Mirtha Elena
Año de publicación
1996
Idioma
español castellano
Tipo de recurso
tesis doctoral
Estado
versión publicada
Colaborador/a o director/a de tesis
Méndez, Beatriz Silvia
Descripción
The polyhydroxyalkanoates (PHAs) are a group of polymers that accumulate in the bacterialcytoplasm forming inclusions called granules. These polymers, biosynthesized from removableresources, can be used as biodegradable plastics. Bacillus megaterium accumulates poly(3-hydroxybutyrate) PHB, a linear homopolymer of D(-)-3-hydroxybutyrate. The granule structureand the biochemical mechanisms involved in their synthesis are not completely understood. In thiswork a collection of B. megaterium mutants deficient in PHB synthesis was obtained. None ofthem showed PHA synthase activity. A system was developed for the study of heterologous genes,using these mutants and the Gram positive and negative bacterial shuttle plasmid pAT19, ascloning vector. In an attempt to understand the role of granule associated proteins and theirinteractions, this system was used to analyze if the 22 Mr protein of B. megaterium, that issupposed to perform the role of a structural protein, could be associated with PHA granulessynthesized by the Alcaligenes eutrophus synthase. The results showed an association to thegranules, whose synthesis was directed by an heterologous synthase.
Fil: Floccari, Mirtha Elena. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Materia
POLI(3-HIDROXIBUTIRATO)
MUTANTES
SINTASA
PROTEÍNAS ASOCIADAS AL GRÁNULO
POLY(3-HYDROXYBUTYRATE)
MUTANTS
SYNTHASE
GRANULE ASSOCIATED PROTEINS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
tesis:tesis_n2818_Floccari
Acceder
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oai_identifier_str tesis:tesis_n2818_Floccari
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Estudios genéticos de la biosíntesis de Poli(3-Hidroxibutirato)PHB, en Bacillus megaterium. Obtención y caracterización de mutantes y expresión heteróloga de genes phbStudies on the biosynthesis of Poly(3-Hydroxybutyrate), PHB, in Bacillus megaterium. Isolation and characterization of mutants and heterologous expression of phb genesFloccari, Mirtha ElenaPOLI(3-HIDROXIBUTIRATO)MUTANTESSINTASAPROTEÍNAS ASOCIADAS AL GRÁNULOPOLY(3-HYDROXYBUTYRATE)MUTANTSSYNTHASEGRANULE ASSOCIATED PROTEINSThe polyhydroxyalkanoates (PHAs) are a group of polymers that accumulate in the bacterialcytoplasm forming inclusions called granules. These polymers, biosynthesized from removableresources, can be used as biodegradable plastics. Bacillus megaterium accumulates poly(3-hydroxybutyrate) PHB, a linear homopolymer of D(-)-3-hydroxybutyrate. The granule structureand the biochemical mechanisms involved in their synthesis are not completely understood. In thiswork a collection of B. megaterium mutants deficient in PHB synthesis was obtained. None ofthem showed PHA synthase activity. A system was developed for the study of heterologous genes,using these mutants and the Gram positive and negative bacterial shuttle plasmid pAT19, ascloning vector. In an attempt to understand the role of granule associated proteins and theirinteractions, this system was used to analyze if the 22 Mr protein of B. megaterium, that issupposed to perform the role of a structural protein, could be associated with PHA granulessynthesized by the Alcaligenes eutrophus synthase. The results showed an association to thegranules, whose synthesis was directed by an heterologous synthase.Fil: Floccari, Mirtha Elena. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Universidad de Buenos Aires. Facultad de Ciencias Exactas y NaturalesMéndez, Beatriz Silvia1996info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_db06info:ar-repo/semantics/tesisDoctoralapplication/pdfhttps://hdl.handle.net/20.500.12110/tesis_n2818_Floccarispainfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/arreponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCEN2026-09-24T11:35:35Ztesis:tesis_n2818_FloccariInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962026-09-24 11:35:39.373Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Estudios genéticos de la biosíntesis de Poli(3-Hidroxibutirato)PHB, en Bacillus megaterium. Obtención y caracterización de mutantes y expresión heteróloga de genes phb
Studies on the biosynthesis of Poly(3-Hydroxybutyrate), PHB, in Bacillus megaterium. Isolation and characterization of mutants and heterologous expression of phb genes
title Estudios genéticos de la biosíntesis de Poli(3-Hidroxibutirato)PHB, en Bacillus megaterium. Obtención y caracterización de mutantes y expresión heteróloga de genes phb
spellingShingle Estudios genéticos de la biosíntesis de Poli(3-Hidroxibutirato)PHB, en Bacillus megaterium. Obtención y caracterización de mutantes y expresión heteróloga de genes phb
Floccari, Mirtha Elena
POLI(3-HIDROXIBUTIRATO)
MUTANTES
SINTASA
PROTEÍNAS ASOCIADAS AL GRÁNULO
POLY(3-HYDROXYBUTYRATE)
MUTANTS
SYNTHASE
GRANULE ASSOCIATED PROTEINS
title_short Estudios genéticos de la biosíntesis de Poli(3-Hidroxibutirato)PHB, en Bacillus megaterium. Obtención y caracterización de mutantes y expresión heteróloga de genes phb
title_full Estudios genéticos de la biosíntesis de Poli(3-Hidroxibutirato)PHB, en Bacillus megaterium. Obtención y caracterización de mutantes y expresión heteróloga de genes phb
title_fullStr Estudios genéticos de la biosíntesis de Poli(3-Hidroxibutirato)PHB, en Bacillus megaterium. Obtención y caracterización de mutantes y expresión heteróloga de genes phb
title_full_unstemmed Estudios genéticos de la biosíntesis de Poli(3-Hidroxibutirato)PHB, en Bacillus megaterium. Obtención y caracterización de mutantes y expresión heteróloga de genes phb
title_sort Estudios genéticos de la biosíntesis de Poli(3-Hidroxibutirato)PHB, en Bacillus megaterium. Obtención y caracterización de mutantes y expresión heteróloga de genes phb
dc.creator.none.fl_str_mv Floccari, Mirtha Elena
author Floccari, Mirtha Elena
author_facet Floccari, Mirtha Elena
author_role author
dc.contributor.none.fl_str_mv Méndez, Beatriz Silvia
dc.subject.none.fl_str_mv POLI(3-HIDROXIBUTIRATO)
MUTANTES
SINTASA
PROTEÍNAS ASOCIADAS AL GRÁNULO
POLY(3-HYDROXYBUTYRATE)
MUTANTS
SYNTHASE
GRANULE ASSOCIATED PROTEINS
topic POLI(3-HIDROXIBUTIRATO)
MUTANTES
SINTASA
PROTEÍNAS ASOCIADAS AL GRÁNULO
POLY(3-HYDROXYBUTYRATE)
MUTANTS
SYNTHASE
GRANULE ASSOCIATED PROTEINS
dc.description.none.fl_txt_mv The polyhydroxyalkanoates (PHAs) are a group of polymers that accumulate in the bacterialcytoplasm forming inclusions called granules. These polymers, biosynthesized from removableresources, can be used as biodegradable plastics. Bacillus megaterium accumulates poly(3-hydroxybutyrate) PHB, a linear homopolymer of D(-)-3-hydroxybutyrate. The granule structureand the biochemical mechanisms involved in their synthesis are not completely understood. In thiswork a collection of B. megaterium mutants deficient in PHB synthesis was obtained. None ofthem showed PHA synthase activity. A system was developed for the study of heterologous genes,using these mutants and the Gram positive and negative bacterial shuttle plasmid pAT19, ascloning vector. In an attempt to understand the role of granule associated proteins and theirinteractions, this system was used to analyze if the 22 Mr protein of B. megaterium, that issupposed to perform the role of a structural protein, could be associated with PHA granulessynthesized by the Alcaligenes eutrophus synthase. The results showed an association to thegranules, whose synthesis was directed by an heterologous synthase.
Fil: Floccari, Mirtha Elena. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description The polyhydroxyalkanoates (PHAs) are a group of polymers that accumulate in the bacterialcytoplasm forming inclusions called granules. These polymers, biosynthesized from removableresources, can be used as biodegradable plastics. Bacillus megaterium accumulates poly(3-hydroxybutyrate) PHB, a linear homopolymer of D(-)-3-hydroxybutyrate. The granule structureand the biochemical mechanisms involved in their synthesis are not completely understood. In thiswork a collection of B. megaterium mutants deficient in PHB synthesis was obtained. None ofthem showed PHA synthase activity. A system was developed for the study of heterologous genes,using these mutants and the Gram positive and negative bacterial shuttle plasmid pAT19, ascloning vector. In an attempt to understand the role of granule associated proteins and theirinteractions, this system was used to analyze if the 22 Mr protein of B. megaterium, that issupposed to perform the role of a structural protein, could be associated with PHA granulessynthesized by the Alcaligenes eutrophus synthase. The results showed an association to thegranules, whose synthesis was directed by an heterologous synthase.
publishDate 1996
dc.date.none.fl_str_mv 1996
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_db06
info:ar-repo/semantics/tesisDoctoral
format doctoralThesis
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12110/tesis_n2818_Floccari
url https://hdl.handle.net/20.500.12110/tesis_n2818_Floccari
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales
publisher.none.fl_str_mv Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales
dc.source.none.fl_str_mv reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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score 12.733355

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