Polyethylene glycol–Brushite cement: A versatile platform for high-performance electrochemical biosensors

Autores
Hervás Pérez, Juan Pablo; Moyano, Fernando; Izcara, Sergio; Sánchez Paniagua, Marta
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The combination of brushite (dicalcium phosphate dihydrate) and polyethylene glycol (PEG) provides a synergistic platform that integrates the biocompatibility and protein affinity of brushite with the antifouling properties and enzyme-stabilizing effects of PEG. This composite offers an ideal matrix for the development of electrochemical biosensors, enabling efficient enzyme immobilization while preserving catalytic activity. Here, we report the development of a novel electrochemical biosensor based on a PEG–brushite composite for the immobilization of glucose oxidase (GOx) as a model enzyme. The composite is obtained through a simple, rapidand reproducible procedure, yielding a homogeneous and stable colloidal system that enables efficient enzyme incorporation while preserving catalytic activity. The resulting biosensor displayed a wide linear range (1.8 × 10-6–1.2 × 10- 2 M), high sensitivity (50.5 mA⋅M- 1⋅cm- 2), low detection limit (1.7 × 10- 7 M), and rapid response (10 s) toward glucose detection.The biosensor was successfully applied for determination of glucose in clinical samples (artificial saliva and serum) as well as in a food matrix (grape juice), covering both millimolar and micromolar concentration ranges within a single sensing platform. Matrix effects were negligible, as evidence the recoveries values ranging from 93 to 110%. Furthermore, the PEG–brushite biosensor retained approximately 95% of its initial activity after 15 days of refrigerated storage, significantly outperforming brushite-only systems. Overall, these findings demonstrate that PEG–modified brushite provides enhanced stability and sensitivity, establishing this composite as a robust, biocompatible and versatile for practical glucose monitoring and a promising candidate for advanced enzyme-based electrochemical biosensors.
Fil: Hervás Pérez, Juan Pablo. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Moyano, Fernando. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Izcara, Sergio. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Sánchez Paniagua, Marta. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Materia
Brushite
Polyethylengicol
Enzyme
Biosensor
Glucose
Saliva
Serum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/287176
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/287176
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Polyethylene glycol–Brushite cement: A versatile platform for high-performance electrochemical biosensorsHervás Pérez, Juan PabloMoyano, FernandoIzcara, SergioSánchez Paniagua, MartaBrushitePolyethylengicolEnzymeBiosensorGlucoseSalivaSerumhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The combination of brushite (dicalcium phosphate dihydrate) and polyethylene glycol (PEG) provides a synergistic platform that integrates the biocompatibility and protein affinity of brushite with the antifouling properties and enzyme-stabilizing effects of PEG. This composite offers an ideal matrix for the development of electrochemical biosensors, enabling efficient enzyme immobilization while preserving catalytic activity. Here, we report the development of a novel electrochemical biosensor based on a PEG–brushite composite for the immobilization of glucose oxidase (GOx) as a model enzyme. The composite is obtained through a simple, rapidand reproducible procedure, yielding a homogeneous and stable colloidal system that enables efficient enzyme incorporation while preserving catalytic activity. The resulting biosensor displayed a wide linear range (1.8 × 10-6–1.2 × 10- 2 M), high sensitivity (50.5 mA⋅M- 1⋅cm- 2), low detection limit (1.7 × 10- 7 M), and rapid response (10 s) toward glucose detection.The biosensor was successfully applied for determination of glucose in clinical samples (artificial saliva and serum) as well as in a food matrix (grape juice), covering both millimolar and micromolar concentration ranges within a single sensing platform. Matrix effects were negligible, as evidence the recoveries values ranging from 93 to 110%. Furthermore, the PEG–brushite biosensor retained approximately 95% of its initial activity after 15 days of refrigerated storage, significantly outperforming brushite-only systems. Overall, these findings demonstrate that PEG–modified brushite provides enhanced stability and sensitivity, establishing this composite as a robust, biocompatible and versatile for practical glucose monitoring and a promising candidate for advanced enzyme-based electrochemical biosensors.Fil: Hervás Pérez, Juan Pablo. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Moyano, Fernando. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; ArgentinaFil: Izcara, Sergio. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaFil: Sánchez Paniagua, Marta. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; EspañaElsevier Science2026-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/287176Hervás Pérez, Juan Pablo; Moyano, Fernando; Izcara, Sergio; Sánchez Paniagua, Marta; Polyethylene glycol–Brushite cement: A versatile platform for high-performance electrochemical biosensors; Elsevier Science; Microchemical Journal; 224; 4-2026; 117930-1179390026-265XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0026265X2601132Xinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2026.117930info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:30:42Zoai:ri.conicet.gov.ar:11336/287176instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:30:42.801CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Polyethylene glycol–Brushite cement: A versatile platform for high-performance electrochemical biosensors
title Polyethylene glycol–Brushite cement: A versatile platform for high-performance electrochemical biosensors
spellingShingle Polyethylene glycol–Brushite cement: A versatile platform for high-performance electrochemical biosensors
Hervás Pérez, Juan Pablo
Brushite
Polyethylengicol
Enzyme
Biosensor
Glucose
Saliva
Serum
title_short Polyethylene glycol–Brushite cement: A versatile platform for high-performance electrochemical biosensors
title_full Polyethylene glycol–Brushite cement: A versatile platform for high-performance electrochemical biosensors
title_fullStr Polyethylene glycol–Brushite cement: A versatile platform for high-performance electrochemical biosensors
title_full_unstemmed Polyethylene glycol–Brushite cement: A versatile platform for high-performance electrochemical biosensors
title_sort Polyethylene glycol–Brushite cement: A versatile platform for high-performance electrochemical biosensors
dc.creator.none.fl_str_mv Hervás Pérez, Juan Pablo
Moyano, Fernando
Izcara, Sergio
Sánchez Paniagua, Marta
author Hervás Pérez, Juan Pablo
author_facet Hervás Pérez, Juan Pablo
Moyano, Fernando
Izcara, Sergio
Sánchez Paniagua, Marta
author_role author
author2 Moyano, Fernando
Izcara, Sergio
Sánchez Paniagua, Marta
author2_role author
author
author
dc.subject.none.fl_str_mv Brushite
Polyethylengicol
Enzyme
Biosensor
Glucose
Saliva
Serum
topic Brushite
Polyethylengicol
Enzyme
Biosensor
Glucose
Saliva
Serum
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The combination of brushite (dicalcium phosphate dihydrate) and polyethylene glycol (PEG) provides a synergistic platform that integrates the biocompatibility and protein affinity of brushite with the antifouling properties and enzyme-stabilizing effects of PEG. This composite offers an ideal matrix for the development of electrochemical biosensors, enabling efficient enzyme immobilization while preserving catalytic activity. Here, we report the development of a novel electrochemical biosensor based on a PEG–brushite composite for the immobilization of glucose oxidase (GOx) as a model enzyme. The composite is obtained through a simple, rapidand reproducible procedure, yielding a homogeneous and stable colloidal system that enables efficient enzyme incorporation while preserving catalytic activity. The resulting biosensor displayed a wide linear range (1.8 × 10-6–1.2 × 10- 2 M), high sensitivity (50.5 mA⋅M- 1⋅cm- 2), low detection limit (1.7 × 10- 7 M), and rapid response (10 s) toward glucose detection.The biosensor was successfully applied for determination of glucose in clinical samples (artificial saliva and serum) as well as in a food matrix (grape juice), covering both millimolar and micromolar concentration ranges within a single sensing platform. Matrix effects were negligible, as evidence the recoveries values ranging from 93 to 110%. Furthermore, the PEG–brushite biosensor retained approximately 95% of its initial activity after 15 days of refrigerated storage, significantly outperforming brushite-only systems. Overall, these findings demonstrate that PEG–modified brushite provides enhanced stability and sensitivity, establishing this composite as a robust, biocompatible and versatile for practical glucose monitoring and a promising candidate for advanced enzyme-based electrochemical biosensors.
Fil: Hervás Pérez, Juan Pablo. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Moyano, Fernando. Universidad Nacional de Río Cuarto. Instituto para el Desarrollo Agroindustrial y de la Salud. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto para el Desarrollo Agroindustrial y de la Salud; Argentina
Fil: Izcara, Sergio. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
Fil: Sánchez Paniagua, Marta. Universidad Complutense de Madrid. Facultad de Ciencias Químicas; España
description The combination of brushite (dicalcium phosphate dihydrate) and polyethylene glycol (PEG) provides a synergistic platform that integrates the biocompatibility and protein affinity of brushite with the antifouling properties and enzyme-stabilizing effects of PEG. This composite offers an ideal matrix for the development of electrochemical biosensors, enabling efficient enzyme immobilization while preserving catalytic activity. Here, we report the development of a novel electrochemical biosensor based on a PEG–brushite composite for the immobilization of glucose oxidase (GOx) as a model enzyme. The composite is obtained through a simple, rapidand reproducible procedure, yielding a homogeneous and stable colloidal system that enables efficient enzyme incorporation while preserving catalytic activity. The resulting biosensor displayed a wide linear range (1.8 × 10-6–1.2 × 10- 2 M), high sensitivity (50.5 mA⋅M- 1⋅cm- 2), low detection limit (1.7 × 10- 7 M), and rapid response (10 s) toward glucose detection.The biosensor was successfully applied for determination of glucose in clinical samples (artificial saliva and serum) as well as in a food matrix (grape juice), covering both millimolar and micromolar concentration ranges within a single sensing platform. Matrix effects were negligible, as evidence the recoveries values ranging from 93 to 110%. Furthermore, the PEG–brushite biosensor retained approximately 95% of its initial activity after 15 days of refrigerated storage, significantly outperforming brushite-only systems. Overall, these findings demonstrate that PEG–modified brushite provides enhanced stability and sensitivity, establishing this composite as a robust, biocompatible and versatile for practical glucose monitoring and a promising candidate for advanced enzyme-based electrochemical biosensors.
publishDate 2026
dc.date.none.fl_str_mv 2026-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/287176
Hervás Pérez, Juan Pablo; Moyano, Fernando; Izcara, Sergio; Sánchez Paniagua, Marta; Polyethylene glycol–Brushite cement: A versatile platform for high-performance electrochemical biosensors; Elsevier Science; Microchemical Journal; 224; 4-2026; 117930-117939
0026-265X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/287176
identifier_str_mv Hervás Pérez, Juan Pablo; Moyano, Fernando; Izcara, Sergio; Sánchez Paniagua, Marta; Polyethylene glycol–Brushite cement: A versatile platform for high-performance electrochemical biosensors; Elsevier Science; Microchemical Journal; 224; 4-2026; 117930-117939
0026-265X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0026265X2601132X
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.microc.2026.117930
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.265058

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