First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina

Autores
Sebastian, Patrick Stephan; Vaschalde, Paula Josefina; Pelosi, María C.; Flores, Fernando Sebastián; Yedro, Julio; Tarragona, Evelina Luisa
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In November 2022, a male specimen of Subulo gouazoubira was found with signs of obnubilationand recumbency without the ability to maintain sternal or ambulatory posture.Based on the symptoms, serological, biochemical and molecular analyses were performed.The results of the biochemical blood analysis and the clinical signs were compatible withtheileriosis. DNA of Theileria were detected in the blood sample by PCR analysis. Thephylogenetic analysis of the obtained partial sequences of the 18S rDNA gen resulted inthe identification of the detected strain as a Theileria genospecies closely related to Theileriaspp. detected in other deer species. Further, a genospecies of Anaplasma was detected inthe blood sample. This genospecies is located phylogenetically near to Anaplasma phagocytophilum.The results of this study demonstrate co-infection with two novel genospecies ofAnaplasma and Theileria in a clinical case of a free-living S. gouazoubira for the first time inArgentina. However, the pathogenicity of these strains and specific role of S. gouazoubira intheir enzootic transmission cycles remains unclear. To improve epidemiological understanding,assess risks and develop targeted control strategies, future studies should prioritizethe isolation, characterization and cultivation of these genospecies and the assessment ofvector competence.
Fil: Sebastian, Patrick Stephan. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Vaschalde, Paula Josefina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Pelosi, María C.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Flores, Fernando Sebastián. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Yedro, Julio. Provincia de Entre Ríos; Argentina
Fil: Tarragona, Evelina Luisa. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Materia
wildlife
cervidae
piroplasmida
anaplasmataceae
South America
ticks
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/291466
Acceder
id CONICETDig_21808f6e7ddefc27f84516b8de0b975e
oai_identifier_str oai:ri.conicet.gov.ar:11336/291466
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, ArgentinaSebastian, Patrick StephanVaschalde, Paula JosefinaPelosi, María C.Flores, Fernando SebastiánYedro, JulioTarragona, Evelina LuisawildlifecervidaepiroplasmidaanaplasmataceaeSouth Americatickshttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4In November 2022, a male specimen of Subulo gouazoubira was found with signs of obnubilationand recumbency without the ability to maintain sternal or ambulatory posture.Based on the symptoms, serological, biochemical and molecular analyses were performed.The results of the biochemical blood analysis and the clinical signs were compatible withtheileriosis. DNA of Theileria were detected in the blood sample by PCR analysis. Thephylogenetic analysis of the obtained partial sequences of the 18S rDNA gen resulted inthe identification of the detected strain as a Theileria genospecies closely related to Theileriaspp. detected in other deer species. Further, a genospecies of Anaplasma was detected inthe blood sample. This genospecies is located phylogenetically near to Anaplasma phagocytophilum.The results of this study demonstrate co-infection with two novel genospecies ofAnaplasma and Theileria in a clinical case of a free-living S. gouazoubira for the first time inArgentina. However, the pathogenicity of these strains and specific role of S. gouazoubira intheir enzootic transmission cycles remains unclear. To improve epidemiological understanding,assess risks and develop targeted control strategies, future studies should prioritizethe isolation, characterization and cultivation of these genospecies and the assessment ofvector competence.Fil: Sebastian, Patrick Stephan. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; ArgentinaFil: Vaschalde, Paula Josefina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; ArgentinaFil: Pelosi, María C.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Flores, Fernando Sebastián. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Yedro, Julio. Provincia de Entre Ríos; ArgentinaFil: Tarragona, Evelina Luisa. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; ArgentinaMDPI2025-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/291466Sebastian, Patrick Stephan; Vaschalde, Paula Josefina; Pelosi, María C.; Flores, Fernando Sebastián; Yedro, Julio; et al.; First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina; MDPI; wild; 2; 3; 9-2025; 1-103042-4526CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/3042-4526/2/3/35info:eu-repo/semantics/altIdentifier/doi/10.3390/wild2030035info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:49:42Zoai:ri.conicet.gov.ar:11336/291466instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:49:43.005CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina
title First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina
spellingShingle First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina
Sebastian, Patrick Stephan
wildlife
cervidae
piroplasmida
anaplasmataceae
South America
ticks
title_short First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina
title_full First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina
title_fullStr First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina
title_full_unstemmed First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina
title_sort First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina
dc.creator.none.fl_str_mv Sebastian, Patrick Stephan
Vaschalde, Paula Josefina
Pelosi, María C.
Flores, Fernando Sebastián
Yedro, Julio
Tarragona, Evelina Luisa
author Sebastian, Patrick Stephan
author_facet Sebastian, Patrick Stephan
Vaschalde, Paula Josefina
Pelosi, María C.
Flores, Fernando Sebastián
Yedro, Julio
Tarragona, Evelina Luisa
author_role author
author2 Vaschalde, Paula Josefina
Pelosi, María C.
Flores, Fernando Sebastián
Yedro, Julio
Tarragona, Evelina Luisa
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv wildlife
cervidae
piroplasmida
anaplasmataceae
South America
ticks
topic wildlife
cervidae
piroplasmida
anaplasmataceae
South America
ticks
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv In November 2022, a male specimen of Subulo gouazoubira was found with signs of obnubilationand recumbency without the ability to maintain sternal or ambulatory posture.Based on the symptoms, serological, biochemical and molecular analyses were performed.The results of the biochemical blood analysis and the clinical signs were compatible withtheileriosis. DNA of Theileria were detected in the blood sample by PCR analysis. Thephylogenetic analysis of the obtained partial sequences of the 18S rDNA gen resulted inthe identification of the detected strain as a Theileria genospecies closely related to Theileriaspp. detected in other deer species. Further, a genospecies of Anaplasma was detected inthe blood sample. This genospecies is located phylogenetically near to Anaplasma phagocytophilum.The results of this study demonstrate co-infection with two novel genospecies ofAnaplasma and Theileria in a clinical case of a free-living S. gouazoubira for the first time inArgentina. However, the pathogenicity of these strains and specific role of S. gouazoubira intheir enzootic transmission cycles remains unclear. To improve epidemiological understanding,assess risks and develop targeted control strategies, future studies should prioritizethe isolation, characterization and cultivation of these genospecies and the assessment ofvector competence.
Fil: Sebastian, Patrick Stephan. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Vaschalde, Paula Josefina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Fil: Pelosi, María C.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Flores, Fernando Sebastián. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Yedro, Julio. Provincia de Entre Ríos; Argentina
Fil: Tarragona, Evelina Luisa. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
description In November 2022, a male specimen of Subulo gouazoubira was found with signs of obnubilationand recumbency without the ability to maintain sternal or ambulatory posture.Based on the symptoms, serological, biochemical and molecular analyses were performed.The results of the biochemical blood analysis and the clinical signs were compatible withtheileriosis. DNA of Theileria were detected in the blood sample by PCR analysis. Thephylogenetic analysis of the obtained partial sequences of the 18S rDNA gen resulted inthe identification of the detected strain as a Theileria genospecies closely related to Theileriaspp. detected in other deer species. Further, a genospecies of Anaplasma was detected inthe blood sample. This genospecies is located phylogenetically near to Anaplasma phagocytophilum.The results of this study demonstrate co-infection with two novel genospecies ofAnaplasma and Theileria in a clinical case of a free-living S. gouazoubira for the first time inArgentina. However, the pathogenicity of these strains and specific role of S. gouazoubira intheir enzootic transmission cycles remains unclear. To improve epidemiological understanding,assess risks and develop targeted control strategies, future studies should prioritizethe isolation, characterization and cultivation of these genospecies and the assessment ofvector competence.
publishDate 2025
dc.date.none.fl_str_mv 2025-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/291466
Sebastian, Patrick Stephan; Vaschalde, Paula Josefina; Pelosi, María C.; Flores, Fernando Sebastián; Yedro, Julio; et al.; First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina; MDPI; wild; 2; 3; 9-2025; 1-10
3042-4526
CONICET Digital
CONICET
url http://hdl.handle.net/11336/291466
identifier_str_mv Sebastian, Patrick Stephan; Vaschalde, Paula Josefina; Pelosi, María C.; Flores, Fernando Sebastián; Yedro, Julio; et al.; First Molecular Evidence of Theileria and Anaplasma Genospecies in Subulo gouazoubira Exhibiting Clinical Symptoms from Entre Ríos Province, Argentina; MDPI; wild; 2; 3; 9-2025; 1-10
3042-4526
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/3042-4526/2/3/35
info:eu-repo/semantics/altIdentifier/doi/10.3390/wild2030035
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1874774736133685248
score 13.265058

Publicaciones similares