Differential effects of EtOH pre-exposure or acute intoxication on hypoxic ventilatory response, medullary serotonin levels and metabolic parameters in neonate rats

Autores
Segovia, Marisol Magali; Tejerina, David Nolberto; Amigone, José Luis; Farfan, Silvia; Castelli, Gabriela; Macchione, Ana Fabiola
Año de publicación
2025
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Acute ethanol-EtOH intoxication during the neonatal period reduces the hypoxic ventilatory response (HVR) and alters serotonin (5-HT) levels in the medullary 5-HT system. Yet, the impact of EtOH preexposure (pre-EtOH) on acute intoxication remains unclear. We examined how pre-EtOH and acute EtOH interact to affect HVR, medullary 5-HT, and metabolic responses. Pups were pre-exposed to 2.0 or 0.0 g/kg EtOH (ig) on postnatal days (PD) 3, 5, and 7. On PD9, pups received acute EtOH or vehicle and then were subjected to intermittent (IH) or continuous (CH) hypoxia. Brainstem and trunk blood were collected for 5-HT and metabolic analysis. Acute EtOH reduced HVR in both IH and CH, regardless of pre-EtOH. Apneas were fewer in IH than CH, though EtOH reduced apneas under all conditions. All EtOH exposures decreased 5-HT levels, but significantly in pre-EtOH sober pups. Hypoxia induced alkalosis and HVR-driven hypocapnia, but acute EtOH impaired this response inducing acidosis–hypercapnia -exacerbated in pre-EtOH pups under normoxia (sensitization). Hypoxia also enhanced oxygenation, but acute EtOH attenuated this effect. Notably, pre-EtOH pups under IH showed an adaptive profile, like tolerance. These findings reveal both EtOH exposures disrupt neonatal respiratory function: acute EtOH impairs HVR and metabolic compensation, while pre-EtOH alters 5-HT and metabolic parameters, promoting plasticity processes (sensitization and tolerance) to acute intoxication.
Fil: Segovia, Marisol Magali. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina
Fil: Tejerina, David Nolberto. Hospital Privado Universitario de Cordoba.; Argentina
Fil: Amigone, José Luis. Hospital Privado Universitario de Cordoba.; Argentina
Fil: Farfan, Silvia. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
Fil: Castelli, Gabriela. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
Fil: Macchione, Ana Fabiola. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Psicología; Argentina
XL Sociedad Argentina de Investigación en Neurociencias Meeting
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Investigación en Neurociencias
Materia
ETANOL
HVR
METABOLIC PARAMETERS
NEONATES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/289878
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/289878
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differential effects of EtOH pre-exposure or acute intoxication on hypoxic ventilatory response, medullary serotonin levels and metabolic parameters in neonate ratsSegovia, Marisol MagaliTejerina, David NolbertoAmigone, José LuisFarfan, SilviaCastelli, GabrielaMacchione, Ana FabiolaETANOLHVRMETABOLIC PARAMETERSNEONATEShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Acute ethanol-EtOH intoxication during the neonatal period reduces the hypoxic ventilatory response (HVR) and alters serotonin (5-HT) levels in the medullary 5-HT system. Yet, the impact of EtOH preexposure (pre-EtOH) on acute intoxication remains unclear. We examined how pre-EtOH and acute EtOH interact to affect HVR, medullary 5-HT, and metabolic responses. Pups were pre-exposed to 2.0 or 0.0 g/kg EtOH (ig) on postnatal days (PD) 3, 5, and 7. On PD9, pups received acute EtOH or vehicle and then were subjected to intermittent (IH) or continuous (CH) hypoxia. Brainstem and trunk blood were collected for 5-HT and metabolic analysis. Acute EtOH reduced HVR in both IH and CH, regardless of pre-EtOH. Apneas were fewer in IH than CH, though EtOH reduced apneas under all conditions. All EtOH exposures decreased 5-HT levels, but significantly in pre-EtOH sober pups. Hypoxia induced alkalosis and HVR-driven hypocapnia, but acute EtOH impaired this response inducing acidosis–hypercapnia -exacerbated in pre-EtOH pups under normoxia (sensitization). Hypoxia also enhanced oxygenation, but acute EtOH attenuated this effect. Notably, pre-EtOH pups under IH showed an adaptive profile, like tolerance. These findings reveal both EtOH exposures disrupt neonatal respiratory function: acute EtOH impairs HVR and metabolic compensation, while pre-EtOH alters 5-HT and metabolic parameters, promoting plasticity processes (sensitization and tolerance) to acute intoxication.Fil: Segovia, Marisol Magali. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; ArgentinaFil: Tejerina, David Nolberto. Hospital Privado Universitario de Cordoba.; ArgentinaFil: Amigone, José Luis. Hospital Privado Universitario de Cordoba.; ArgentinaFil: Farfan, Silvia. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; ArgentinaFil: Castelli, Gabriela. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; ArgentinaFil: Macchione, Ana Fabiola. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Psicología; ArgentinaXL Sociedad Argentina de Investigación en Neurociencias MeetingCiudad Autónoma de Buenos AiresArgentinaSociedad Argentina de Investigación en NeurocienciasSociedad Argentina de Investigación en Neurociencias2025info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/289878Differential effects of EtOH pre-exposure or acute intoxication on hypoxic ventilatory response, medullary serotonin levels and metabolic parameters in neonate rats; XL Sociedad Argentina de Investigación en Neurociencias Meeting; Ciudad Autónoma de Buenos Aires; Argentina; 2025; 55-55CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://csan2025.saneurociencias.org.ar/wp-content/uploads/2025/10/SAN2025-Posters-EBOOK-Final.pdf?fbclid=PAZnRzaANNB21leHRuA2FlbQIxMQABp2BX6siytE0G1a7xuvCfceb3AWzyPpd33UiygOhK6GQKXC_2fDuQQu3120fX_aem_-sAE4u_UQtV5zi0txEQVlwNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:49:27Zoai:ri.conicet.gov.ar:11336/289878instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:49:27.385CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential effects of EtOH pre-exposure or acute intoxication on hypoxic ventilatory response, medullary serotonin levels and metabolic parameters in neonate rats
title Differential effects of EtOH pre-exposure or acute intoxication on hypoxic ventilatory response, medullary serotonin levels and metabolic parameters in neonate rats
spellingShingle Differential effects of EtOH pre-exposure or acute intoxication on hypoxic ventilatory response, medullary serotonin levels and metabolic parameters in neonate rats
Segovia, Marisol Magali
ETANOL
HVR
METABOLIC PARAMETERS
NEONATES
title_short Differential effects of EtOH pre-exposure or acute intoxication on hypoxic ventilatory response, medullary serotonin levels and metabolic parameters in neonate rats
title_full Differential effects of EtOH pre-exposure or acute intoxication on hypoxic ventilatory response, medullary serotonin levels and metabolic parameters in neonate rats
title_fullStr Differential effects of EtOH pre-exposure or acute intoxication on hypoxic ventilatory response, medullary serotonin levels and metabolic parameters in neonate rats
title_full_unstemmed Differential effects of EtOH pre-exposure or acute intoxication on hypoxic ventilatory response, medullary serotonin levels and metabolic parameters in neonate rats
title_sort Differential effects of EtOH pre-exposure or acute intoxication on hypoxic ventilatory response, medullary serotonin levels and metabolic parameters in neonate rats
dc.creator.none.fl_str_mv Segovia, Marisol Magali
Tejerina, David Nolberto
Amigone, José Luis
Farfan, Silvia
Castelli, Gabriela
Macchione, Ana Fabiola
author Segovia, Marisol Magali
author_facet Segovia, Marisol Magali
Tejerina, David Nolberto
Amigone, José Luis
Farfan, Silvia
Castelli, Gabriela
Macchione, Ana Fabiola
author_role author
author2 Tejerina, David Nolberto
Amigone, José Luis
Farfan, Silvia
Castelli, Gabriela
Macchione, Ana Fabiola
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ETANOL
HVR
METABOLIC PARAMETERS
NEONATES
topic ETANOL
HVR
METABOLIC PARAMETERS
NEONATES
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Acute ethanol-EtOH intoxication during the neonatal period reduces the hypoxic ventilatory response (HVR) and alters serotonin (5-HT) levels in the medullary 5-HT system. Yet, the impact of EtOH preexposure (pre-EtOH) on acute intoxication remains unclear. We examined how pre-EtOH and acute EtOH interact to affect HVR, medullary 5-HT, and metabolic responses. Pups were pre-exposed to 2.0 or 0.0 g/kg EtOH (ig) on postnatal days (PD) 3, 5, and 7. On PD9, pups received acute EtOH or vehicle and then were subjected to intermittent (IH) or continuous (CH) hypoxia. Brainstem and trunk blood were collected for 5-HT and metabolic analysis. Acute EtOH reduced HVR in both IH and CH, regardless of pre-EtOH. Apneas were fewer in IH than CH, though EtOH reduced apneas under all conditions. All EtOH exposures decreased 5-HT levels, but significantly in pre-EtOH sober pups. Hypoxia induced alkalosis and HVR-driven hypocapnia, but acute EtOH impaired this response inducing acidosis–hypercapnia -exacerbated in pre-EtOH pups under normoxia (sensitization). Hypoxia also enhanced oxygenation, but acute EtOH attenuated this effect. Notably, pre-EtOH pups under IH showed an adaptive profile, like tolerance. These findings reveal both EtOH exposures disrupt neonatal respiratory function: acute EtOH impairs HVR and metabolic compensation, while pre-EtOH alters 5-HT and metabolic parameters, promoting plasticity processes (sensitization and tolerance) to acute intoxication.
Fil: Segovia, Marisol Magali. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina
Fil: Tejerina, David Nolberto. Hospital Privado Universitario de Cordoba.; Argentina
Fil: Amigone, José Luis. Hospital Privado Universitario de Cordoba.; Argentina
Fil: Farfan, Silvia. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
Fil: Castelli, Gabriela. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
Fil: Macchione, Ana Fabiola. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina. Universidad Nacional de Córdoba. Facultad de Psicología; Argentina
XL Sociedad Argentina de Investigación en Neurociencias Meeting
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina de Investigación en Neurociencias
description Acute ethanol-EtOH intoxication during the neonatal period reduces the hypoxic ventilatory response (HVR) and alters serotonin (5-HT) levels in the medullary 5-HT system. Yet, the impact of EtOH preexposure (pre-EtOH) on acute intoxication remains unclear. We examined how pre-EtOH and acute EtOH interact to affect HVR, medullary 5-HT, and metabolic responses. Pups were pre-exposed to 2.0 or 0.0 g/kg EtOH (ig) on postnatal days (PD) 3, 5, and 7. On PD9, pups received acute EtOH or vehicle and then were subjected to intermittent (IH) or continuous (CH) hypoxia. Brainstem and trunk blood were collected for 5-HT and metabolic analysis. Acute EtOH reduced HVR in both IH and CH, regardless of pre-EtOH. Apneas were fewer in IH than CH, though EtOH reduced apneas under all conditions. All EtOH exposures decreased 5-HT levels, but significantly in pre-EtOH sober pups. Hypoxia induced alkalosis and HVR-driven hypocapnia, but acute EtOH impaired this response inducing acidosis–hypercapnia -exacerbated in pre-EtOH pups under normoxia (sensitization). Hypoxia also enhanced oxygenation, but acute EtOH attenuated this effect. Notably, pre-EtOH pups under IH showed an adaptive profile, like tolerance. These findings reveal both EtOH exposures disrupt neonatal respiratory function: acute EtOH impairs HVR and metabolic compensation, while pre-EtOH alters 5-HT and metabolic parameters, promoting plasticity processes (sensitization and tolerance) to acute intoxication.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/289878
Differential effects of EtOH pre-exposure or acute intoxication on hypoxic ventilatory response, medullary serotonin levels and metabolic parameters in neonate rats; XL Sociedad Argentina de Investigación en Neurociencias Meeting; Ciudad Autónoma de Buenos Aires; Argentina; 2025; 55-55
CONICET Digital
CONICET
url http://hdl.handle.net/11336/289878
identifier_str_mv Differential effects of EtOH pre-exposure or acute intoxication on hypoxic ventilatory response, medullary serotonin levels and metabolic parameters in neonate rats; XL Sociedad Argentina de Investigación en Neurociencias Meeting; Ciudad Autónoma de Buenos Aires; Argentina; 2025; 55-55
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://csan2025.saneurociencias.org.ar/wp-content/uploads/2025/10/SAN2025-Posters-EBOOK-Final.pdf?fbclid=PAZnRzaANNB21leHRuA2FlbQIxMQABp2BX6siytE0G1a7xuvCfceb3AWzyPpd33UiygOhK6GQKXC_2fDuQQu3120fX_aem_-sAE4u_UQtV5zi0txEQVlw
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24418

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