Antioxidant and Histopathological Effects of Paraquat and Fluroxypyr Herbicides on the Apple Snail Pomacea canaliculata (Lamarck, 1822)

Autores
Campoy Díaz, Alejandra Daniela; Vega, Israel Aníbal; Giraud Billoud, Maximiliano German
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Argentina is among the top consumers of herbicides, yet studies on their environmental and health impact remain scarce. This work aimed to evaluate the effects of herbicide exposure on Pomacea canaliculata as potential biomarkers of contamination. Specifically, we investigated whether paraquat (Pq) and fluroxypyr (Fx) alter enzymatic antioxidant defenses in tissues following acute exposure and induce histological modifications in the digestive gland (DG), particularly in symbiotic corpuscles, after chronic exposure. The nominal no-observed-effect concentration on lethality (NOECL) values were 3.62 µg/g dry mass (DM) for Pq and 10.42 µg/g DM for Fx. After acute exposure, superoxide dismutase activity decreased in the DG but increased in the kidney for both herbicides. Catalase activity decreased in the gills but increased in the kidneys of exposed snails, while glutathione-S-transferase activity increased in the DG and kidney after Pq exposure. Following chronic exposure (Pq: 1.45 µg/g DM; Fx: 6.94 µg/g DM), epithelial thickening and vacuolization were observed in Fx-exposed snails. Morphometric analysis of the DG showed that Pq reduced the epithelial occupancy of the symbiont’s vegetative form while increasing its cystic form. These findings indicate that both herbicides impact antioxidant defenses, DG function and host–symbiont interactions, reinforcing the suitability of P. canaliculata as bioindicator organisms.
Fil: Campoy Díaz, Alejandra Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; Argentina. Universidad Nacional de Villa Mercedes; Argentina
Fil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; Argentina
Fil: Giraud Billoud, Maximiliano German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; Argentina. Universidad Nacional de Villa Mercedes; Argentina
Materia
Aquatic contamination
Agrochemicals
Pesticides
Oxidative stress
Symbiosis
Pomacea canaliculata
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/291897
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/291897
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antioxidant and Histopathological Effects of Paraquat and Fluroxypyr Herbicides on the Apple Snail Pomacea canaliculata (Lamarck, 1822)Campoy Díaz, Alejandra DanielaVega, Israel AníbalGiraud Billoud, Maximiliano GermanAquatic contaminationAgrochemicalsPesticidesOxidative stressSymbiosisPomacea canaliculatahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Argentina is among the top consumers of herbicides, yet studies on their environmental and health impact remain scarce. This work aimed to evaluate the effects of herbicide exposure on Pomacea canaliculata as potential biomarkers of contamination. Specifically, we investigated whether paraquat (Pq) and fluroxypyr (Fx) alter enzymatic antioxidant defenses in tissues following acute exposure and induce histological modifications in the digestive gland (DG), particularly in symbiotic corpuscles, after chronic exposure. The nominal no-observed-effect concentration on lethality (NOECL) values were 3.62 µg/g dry mass (DM) for Pq and 10.42 µg/g DM for Fx. After acute exposure, superoxide dismutase activity decreased in the DG but increased in the kidney for both herbicides. Catalase activity decreased in the gills but increased in the kidneys of exposed snails, while glutathione-S-transferase activity increased in the DG and kidney after Pq exposure. Following chronic exposure (Pq: 1.45 µg/g DM; Fx: 6.94 µg/g DM), epithelial thickening and vacuolization were observed in Fx-exposed snails. Morphometric analysis of the DG showed that Pq reduced the epithelial occupancy of the symbiont’s vegetative form while increasing its cystic form. These findings indicate that both herbicides impact antioxidant defenses, DG function and host–symbiont interactions, reinforcing the suitability of P. canaliculata as bioindicator organisms.Fil: Campoy Díaz, Alejandra Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; Argentina. Universidad Nacional de Villa Mercedes; ArgentinaFil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; ArgentinaFil: Giraud Billoud, Maximiliano German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; Argentina. Universidad Nacional de Villa Mercedes; ArgentinaMultidisciplinary Digital Publishing Institute2025-05-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/291897Campoy Díaz, Alejandra Daniela; Vega, Israel Aníbal; Giraud Billoud, Maximiliano German; Antioxidant and Histopathological Effects of Paraquat and Fluroxypyr Herbicides on the Apple Snail Pomacea canaliculata (Lamarck, 1822); Multidisciplinary Digital Publishing Institute; Stresses; 5; 2; 16-5-2025; 1-162673-7140CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2673-7140/5/2/33info:eu-repo/semantics/altIdentifier/doi/10.3390/stresses5020033info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:53:46Zoai:ri.conicet.gov.ar:11336/291897instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:53:46.658CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antioxidant and Histopathological Effects of Paraquat and Fluroxypyr Herbicides on the Apple Snail Pomacea canaliculata (Lamarck, 1822)
title Antioxidant and Histopathological Effects of Paraquat and Fluroxypyr Herbicides on the Apple Snail Pomacea canaliculata (Lamarck, 1822)
spellingShingle Antioxidant and Histopathological Effects of Paraquat and Fluroxypyr Herbicides on the Apple Snail Pomacea canaliculata (Lamarck, 1822)
Campoy Díaz, Alejandra Daniela
Aquatic contamination
Agrochemicals
Pesticides
Oxidative stress
Symbiosis
Pomacea canaliculata
title_short Antioxidant and Histopathological Effects of Paraquat and Fluroxypyr Herbicides on the Apple Snail Pomacea canaliculata (Lamarck, 1822)
title_full Antioxidant and Histopathological Effects of Paraquat and Fluroxypyr Herbicides on the Apple Snail Pomacea canaliculata (Lamarck, 1822)
title_fullStr Antioxidant and Histopathological Effects of Paraquat and Fluroxypyr Herbicides on the Apple Snail Pomacea canaliculata (Lamarck, 1822)
title_full_unstemmed Antioxidant and Histopathological Effects of Paraquat and Fluroxypyr Herbicides on the Apple Snail Pomacea canaliculata (Lamarck, 1822)
title_sort Antioxidant and Histopathological Effects of Paraquat and Fluroxypyr Herbicides on the Apple Snail Pomacea canaliculata (Lamarck, 1822)
dc.creator.none.fl_str_mv Campoy Díaz, Alejandra Daniela
Vega, Israel Aníbal
Giraud Billoud, Maximiliano German
author Campoy Díaz, Alejandra Daniela
author_facet Campoy Díaz, Alejandra Daniela
Vega, Israel Aníbal
Giraud Billoud, Maximiliano German
author_role author
author2 Vega, Israel Aníbal
Giraud Billoud, Maximiliano German
author2_role author
author
dc.subject.none.fl_str_mv Aquatic contamination
Agrochemicals
Pesticides
Oxidative stress
Symbiosis
Pomacea canaliculata
topic Aquatic contamination
Agrochemicals
Pesticides
Oxidative stress
Symbiosis
Pomacea canaliculata
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Argentina is among the top consumers of herbicides, yet studies on their environmental and health impact remain scarce. This work aimed to evaluate the effects of herbicide exposure on Pomacea canaliculata as potential biomarkers of contamination. Specifically, we investigated whether paraquat (Pq) and fluroxypyr (Fx) alter enzymatic antioxidant defenses in tissues following acute exposure and induce histological modifications in the digestive gland (DG), particularly in symbiotic corpuscles, after chronic exposure. The nominal no-observed-effect concentration on lethality (NOECL) values were 3.62 µg/g dry mass (DM) for Pq and 10.42 µg/g DM for Fx. After acute exposure, superoxide dismutase activity decreased in the DG but increased in the kidney for both herbicides. Catalase activity decreased in the gills but increased in the kidneys of exposed snails, while glutathione-S-transferase activity increased in the DG and kidney after Pq exposure. Following chronic exposure (Pq: 1.45 µg/g DM; Fx: 6.94 µg/g DM), epithelial thickening and vacuolization were observed in Fx-exposed snails. Morphometric analysis of the DG showed that Pq reduced the epithelial occupancy of the symbiont’s vegetative form while increasing its cystic form. These findings indicate that both herbicides impact antioxidant defenses, DG function and host–symbiont interactions, reinforcing the suitability of P. canaliculata as bioindicator organisms.
Fil: Campoy Díaz, Alejandra Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; Argentina. Universidad Nacional de Villa Mercedes; Argentina
Fil: Vega, Israel Aníbal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; Argentina
Fil: Giraud Billoud, Maximiliano German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Fisiología; Argentina. Universidad Nacional de Villa Mercedes; Argentina
description Argentina is among the top consumers of herbicides, yet studies on their environmental and health impact remain scarce. This work aimed to evaluate the effects of herbicide exposure on Pomacea canaliculata as potential biomarkers of contamination. Specifically, we investigated whether paraquat (Pq) and fluroxypyr (Fx) alter enzymatic antioxidant defenses in tissues following acute exposure and induce histological modifications in the digestive gland (DG), particularly in symbiotic corpuscles, after chronic exposure. The nominal no-observed-effect concentration on lethality (NOECL) values were 3.62 µg/g dry mass (DM) for Pq and 10.42 µg/g DM for Fx. After acute exposure, superoxide dismutase activity decreased in the DG but increased in the kidney for both herbicides. Catalase activity decreased in the gills but increased in the kidneys of exposed snails, while glutathione-S-transferase activity increased in the DG and kidney after Pq exposure. Following chronic exposure (Pq: 1.45 µg/g DM; Fx: 6.94 µg/g DM), epithelial thickening and vacuolization were observed in Fx-exposed snails. Morphometric analysis of the DG showed that Pq reduced the epithelial occupancy of the symbiont’s vegetative form while increasing its cystic form. These findings indicate that both herbicides impact antioxidant defenses, DG function and host–symbiont interactions, reinforcing the suitability of P. canaliculata as bioindicator organisms.
publishDate 2025
dc.date.none.fl_str_mv 2025-05-16
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/291897
Campoy Díaz, Alejandra Daniela; Vega, Israel Aníbal; Giraud Billoud, Maximiliano German; Antioxidant and Histopathological Effects of Paraquat and Fluroxypyr Herbicides on the Apple Snail Pomacea canaliculata (Lamarck, 1822); Multidisciplinary Digital Publishing Institute; Stresses; 5; 2; 16-5-2025; 1-16
2673-7140
CONICET Digital
CONICET
url http://hdl.handle.net/11336/291897
identifier_str_mv Campoy Díaz, Alejandra Daniela; Vega, Israel Aníbal; Giraud Billoud, Maximiliano German; Antioxidant and Histopathological Effects of Paraquat and Fluroxypyr Herbicides on the Apple Snail Pomacea canaliculata (Lamarck, 1822); Multidisciplinary Digital Publishing Institute; Stresses; 5; 2; 16-5-2025; 1-16
2673-7140
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2673-7140/5/2/33
info:eu-repo/semantics/altIdentifier/doi/10.3390/stresses5020033
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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