Dendritic cell phagosomes recruit GRASP55 for export of antigen-loaded MHC molecules

Autores
Cebrián, José Ignacio; Dinamarca, Sofía; Pena Rodríguez, María Jesús; Priego, Elena; Brouwers, Nathalie; Barends, Martina; Brunnberg, Jamina; Tampé, Robert; Blanchard, Nicolas; Sancho, David; Malhotra, Vivek
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Dendritic cells (DCs) present exogenous antigens via major histocompatibility complex class I (MHC-I) and MHC class II (MHC-II) molecules, activating CD8+ and CD4+ T cells. A critical but poorly understood step in this process is the trafficking of peptide-loaded MHC molecules from the endocytic system to the cell surface. In this study, we demonstrate that the Golgi reassembly-stacking protein of 55 kDa (GRASP55), which has been shown to have no role in stacking, is essential for antigen presentation. Using soluble, bead-coated, and bacterial-bound antigens, we found significantly impaired exogenous antigen presentation in GRASP55 deficient bone-marrow-derived DCs (BMDCs). Notably, GRASP55 was recruited to late phagosomes, and our data suggest that it is crucial for sorting MHC-I and MHC-II molecules, facilitating their trafficking to the plasma membrane. Our findings highlight the vital role of GRASP55 in the intracellular transport of MHC molecules bound to their respective peptides during exogenous antigen presentation.
Fil: Cebrián, José Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Barcelona Institute Of Science And Technology.; España
Fil: Dinamarca, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Barcelona Institute Of Science And Technology.; España
Fil: Pena Rodríguez, María Jesús. Barcelona Institute Of Science And Technology.; España
Fil: Priego, Elena. Centro Nacional de Investigaciones Cardiovasculares; España
Fil: Brouwers, Nathalie. Barcelona Institute Of Science And Technology.; España
Fil: Barends, Martina. Goethe University Frankfurt; Alemania
Fil: Brunnberg, Jamina. Goethe University Frankfurt; Alemania
Fil: Tampé, Robert. Goethe University Frankfurt; Alemania
Fil: Blanchard, Nicolas. Centre National de la Recherche Scientifique; Francia
Fil: Sancho, David. Centro Nacional de Investigaciones Cardiovasculares; España
Fil: Malhotra, Vivek. Barcelona Institute Of Science And Technology.; España
Materia
DENDRITIC CELLS
GRASP55
ANTIGEN PRESENTATION
MHC TRANSPORT
PHAGOSOMES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/290728
Acceder
id CONICETDig_3632d2890b8fc95051d354304fcbd602
oai_identifier_str oai:ri.conicet.gov.ar:11336/290728
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Dendritic cell phagosomes recruit GRASP55 for export of antigen-loaded MHC moleculesCebrián, José IgnacioDinamarca, SofíaPena Rodríguez, María JesúsPriego, ElenaBrouwers, NathalieBarends, MartinaBrunnberg, JaminaTampé, RobertBlanchard, NicolasSancho, DavidMalhotra, VivekDENDRITIC CELLSGRASP55ANTIGEN PRESENTATIONMHC TRANSPORTPHAGOSOMEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1https://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Dendritic cells (DCs) present exogenous antigens via major histocompatibility complex class I (MHC-I) and MHC class II (MHC-II) molecules, activating CD8+ and CD4+ T cells. A critical but poorly understood step in this process is the trafficking of peptide-loaded MHC molecules from the endocytic system to the cell surface. In this study, we demonstrate that the Golgi reassembly-stacking protein of 55 kDa (GRASP55), which has been shown to have no role in stacking, is essential for antigen presentation. Using soluble, bead-coated, and bacterial-bound antigens, we found significantly impaired exogenous antigen presentation in GRASP55 deficient bone-marrow-derived DCs (BMDCs). Notably, GRASP55 was recruited to late phagosomes, and our data suggest that it is crucial for sorting MHC-I and MHC-II molecules, facilitating their trafficking to the plasma membrane. Our findings highlight the vital role of GRASP55 in the intracellular transport of MHC molecules bound to their respective peptides during exogenous antigen presentation.Fil: Cebrián, José Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Barcelona Institute Of Science And Technology.; EspañaFil: Dinamarca, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Barcelona Institute Of Science And Technology.; EspañaFil: Pena Rodríguez, María Jesús. Barcelona Institute Of Science And Technology.; EspañaFil: Priego, Elena. Centro Nacional de Investigaciones Cardiovasculares; EspañaFil: Brouwers, Nathalie. Barcelona Institute Of Science And Technology.; EspañaFil: Barends, Martina. Goethe University Frankfurt; AlemaniaFil: Brunnberg, Jamina. Goethe University Frankfurt; AlemaniaFil: Tampé, Robert. Goethe University Frankfurt; AlemaniaFil: Blanchard, Nicolas. Centre National de la Recherche Scientifique; FranciaFil: Sancho, David. Centro Nacional de Investigaciones Cardiovasculares; EspañaFil: Malhotra, Vivek. Barcelona Institute Of Science And Technology.; EspañaElsevier2025-02-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/290728Cebrián, José Ignacio; Dinamarca, Sofía; Pena Rodríguez, María Jesús; Priego, Elena; Brouwers, Nathalie; et al.; Dendritic cell phagosomes recruit GRASP55 for export of antigen-loaded MHC molecules; Elsevier; Cell Reports; 44; 2; 25-2-2025; 1-222211-1247CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2211124725001044info:eu-repo/semantics/altIdentifier/doi/10.1016/j.celrep.2025.115333info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:52:44Zoai:ri.conicet.gov.ar:11336/290728instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:52:44.715CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Dendritic cell phagosomes recruit GRASP55 for export of antigen-loaded MHC molecules
title Dendritic cell phagosomes recruit GRASP55 for export of antigen-loaded MHC molecules
spellingShingle Dendritic cell phagosomes recruit GRASP55 for export of antigen-loaded MHC molecules
Cebrián, José Ignacio
DENDRITIC CELLS
GRASP55
ANTIGEN PRESENTATION
MHC TRANSPORT
PHAGOSOMES
title_short Dendritic cell phagosomes recruit GRASP55 for export of antigen-loaded MHC molecules
title_full Dendritic cell phagosomes recruit GRASP55 for export of antigen-loaded MHC molecules
title_fullStr Dendritic cell phagosomes recruit GRASP55 for export of antigen-loaded MHC molecules
title_full_unstemmed Dendritic cell phagosomes recruit GRASP55 for export of antigen-loaded MHC molecules
title_sort Dendritic cell phagosomes recruit GRASP55 for export of antigen-loaded MHC molecules
dc.creator.none.fl_str_mv Cebrián, José Ignacio
Dinamarca, Sofía
Pena Rodríguez, María Jesús
Priego, Elena
Brouwers, Nathalie
Barends, Martina
Brunnberg, Jamina
Tampé, Robert
Blanchard, Nicolas
Sancho, David
Malhotra, Vivek
author Cebrián, José Ignacio
author_facet Cebrián, José Ignacio
Dinamarca, Sofía
Pena Rodríguez, María Jesús
Priego, Elena
Brouwers, Nathalie
Barends, Martina
Brunnberg, Jamina
Tampé, Robert
Blanchard, Nicolas
Sancho, David
Malhotra, Vivek
author_role author
author2 Dinamarca, Sofía
Pena Rodríguez, María Jesús
Priego, Elena
Brouwers, Nathalie
Barends, Martina
Brunnberg, Jamina
Tampé, Robert
Blanchard, Nicolas
Sancho, David
Malhotra, Vivek
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DENDRITIC CELLS
GRASP55
ANTIGEN PRESENTATION
MHC TRANSPORT
PHAGOSOMES
topic DENDRITIC CELLS
GRASP55
ANTIGEN PRESENTATION
MHC TRANSPORT
PHAGOSOMES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Dendritic cells (DCs) present exogenous antigens via major histocompatibility complex class I (MHC-I) and MHC class II (MHC-II) molecules, activating CD8+ and CD4+ T cells. A critical but poorly understood step in this process is the trafficking of peptide-loaded MHC molecules from the endocytic system to the cell surface. In this study, we demonstrate that the Golgi reassembly-stacking protein of 55 kDa (GRASP55), which has been shown to have no role in stacking, is essential for antigen presentation. Using soluble, bead-coated, and bacterial-bound antigens, we found significantly impaired exogenous antigen presentation in GRASP55 deficient bone-marrow-derived DCs (BMDCs). Notably, GRASP55 was recruited to late phagosomes, and our data suggest that it is crucial for sorting MHC-I and MHC-II molecules, facilitating their trafficking to the plasma membrane. Our findings highlight the vital role of GRASP55 in the intracellular transport of MHC molecules bound to their respective peptides during exogenous antigen presentation.
Fil: Cebrián, José Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Barcelona Institute Of Science And Technology.; España
Fil: Dinamarca, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina. Barcelona Institute Of Science And Technology.; España
Fil: Pena Rodríguez, María Jesús. Barcelona Institute Of Science And Technology.; España
Fil: Priego, Elena. Centro Nacional de Investigaciones Cardiovasculares; España
Fil: Brouwers, Nathalie. Barcelona Institute Of Science And Technology.; España
Fil: Barends, Martina. Goethe University Frankfurt; Alemania
Fil: Brunnberg, Jamina. Goethe University Frankfurt; Alemania
Fil: Tampé, Robert. Goethe University Frankfurt; Alemania
Fil: Blanchard, Nicolas. Centre National de la Recherche Scientifique; Francia
Fil: Sancho, David. Centro Nacional de Investigaciones Cardiovasculares; España
Fil: Malhotra, Vivek. Barcelona Institute Of Science And Technology.; España
description Dendritic cells (DCs) present exogenous antigens via major histocompatibility complex class I (MHC-I) and MHC class II (MHC-II) molecules, activating CD8+ and CD4+ T cells. A critical but poorly understood step in this process is the trafficking of peptide-loaded MHC molecules from the endocytic system to the cell surface. In this study, we demonstrate that the Golgi reassembly-stacking protein of 55 kDa (GRASP55), which has been shown to have no role in stacking, is essential for antigen presentation. Using soluble, bead-coated, and bacterial-bound antigens, we found significantly impaired exogenous antigen presentation in GRASP55 deficient bone-marrow-derived DCs (BMDCs). Notably, GRASP55 was recruited to late phagosomes, and our data suggest that it is crucial for sorting MHC-I and MHC-II molecules, facilitating their trafficking to the plasma membrane. Our findings highlight the vital role of GRASP55 in the intracellular transport of MHC molecules bound to their respective peptides during exogenous antigen presentation.
publishDate 2025
dc.date.none.fl_str_mv 2025-02-25
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/290728
Cebrián, José Ignacio; Dinamarca, Sofía; Pena Rodríguez, María Jesús; Priego, Elena; Brouwers, Nathalie; et al.; Dendritic cell phagosomes recruit GRASP55 for export of antigen-loaded MHC molecules; Elsevier; Cell Reports; 44; 2; 25-2-2025; 1-22
2211-1247
CONICET Digital
CONICET
url http://hdl.handle.net/11336/290728
identifier_str_mv Cebrián, José Ignacio; Dinamarca, Sofía; Pena Rodríguez, María Jesús; Priego, Elena; Brouwers, Nathalie; et al.; Dendritic cell phagosomes recruit GRASP55 for export of antigen-loaded MHC molecules; Elsevier; Cell Reports; 44; 2; 25-2-2025; 1-22
2211-1247
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2211124725001044
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.celrep.2025.115333
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24418

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