Specific interaction between E2F1 and Sp1 regulates the expression of murine CTP:phosphocholine cytidylyltransferase alpha during the S phase

Autores
Elena, Claudia Edicht; Banchio, Claudia Elena
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
CTP:phosphocholine cytidylyltransferase alpha (CCTα) is a key enzyme for phosphatidylcholine biosynthesis in mammalian cells. This enzyme plays an essential role in all processes that require membrane biosynthesis such as cell proliferation and viability. Thus, CCTα activity and expression fluctuate during the cell cycle to achieve PtdCho requirements. We demonstrated, for the first time, that CCTα is localized in the nucleus in cells transiting the S phase, whereas it is localized in the cytoplasm of G0-arrested cells, suggesting a specific role of nuclear CCTα during the S phase. We also investigated how E2F1 influences the regulation of the CCTα-promoter during the S phase; we demonstrated that E2F1 is necessary, but not sufficient, to activate CCTα expression when this factor is over-expressed. However, when E2F1 and Sp1 were over-expressed, the transcription from the CCTα-promoter reporter construct was super-activated. Transient transfection studies demonstrated that E2F1 could superactivate Sp1-dependent transcription in a promoter containing only the Sp1 binding sites “B” or “C”, and that Sp1 could activate Sp1-dependent transcription in a promoter containing the E2F site, thus, further demonstrating a functional interaction of these factors. In conclusion, the present results allowed us to portray the clearest picture of the CCTα-gene expression in proliferating cells, and understand the mechanism by which cells coordinate cell cycle progression with the requirement for phosphatidylcholine.
Fil: Elena, Claudia Edicht. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Banchio, Claudia Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Materia
cell cycle
proliferation
phospholipids
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/284288
Acceder
id CONICETDig_53de5ce6f11c3a4e14f76fd19b285041
oai_identifier_str oai:ri.conicet.gov.ar:11336/284288
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Specific interaction between E2F1 and Sp1 regulates the expression of murine CTP:phosphocholine cytidylyltransferase alpha during the S phaseElena, Claudia EdichtBanchio, Claudia Elenacell cycleproliferationphospholipidshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1CTP:phosphocholine cytidylyltransferase alpha (CCTα) is a key enzyme for phosphatidylcholine biosynthesis in mammalian cells. This enzyme plays an essential role in all processes that require membrane biosynthesis such as cell proliferation and viability. Thus, CCTα activity and expression fluctuate during the cell cycle to achieve PtdCho requirements. We demonstrated, for the first time, that CCTα is localized in the nucleus in cells transiting the S phase, whereas it is localized in the cytoplasm of G0-arrested cells, suggesting a specific role of nuclear CCTα during the S phase. We also investigated how E2F1 influences the regulation of the CCTα-promoter during the S phase; we demonstrated that E2F1 is necessary, but not sufficient, to activate CCTα expression when this factor is over-expressed. However, when E2F1 and Sp1 were over-expressed, the transcription from the CCTα-promoter reporter construct was super-activated. Transient transfection studies demonstrated that E2F1 could superactivate Sp1-dependent transcription in a promoter containing only the Sp1 binding sites “B” or “C”, and that Sp1 could activate Sp1-dependent transcription in a promoter containing the E2F site, thus, further demonstrating a functional interaction of these factors. In conclusion, the present results allowed us to portray the clearest picture of the CCTα-gene expression in proliferating cells, and understand the mechanism by which cells coordinate cell cycle progression with the requirement for phosphatidylcholine.Fil: Elena, Claudia Edicht. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Banchio, Claudia Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaElsevier Science2010-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/284288Elena, Claudia Edicht; Banchio, Claudia Elena; Specific interaction between E2F1 and Sp1 regulates the expression of murine CTP:phosphocholine cytidylyltransferase alpha during the S phase; Elsevier Science; Biochimica Et Biophysica Acta - Molecular and Cell Biology of Lipids; 1801; 4; 4-2010; 537-5461388-1981CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1388198110000065info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbalip.2010.01.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:35:09Zoai:ri.conicet.gov.ar:11336/284288instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:35:09.701CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Specific interaction between E2F1 and Sp1 regulates the expression of murine CTP:phosphocholine cytidylyltransferase alpha during the S phase
title Specific interaction between E2F1 and Sp1 regulates the expression of murine CTP:phosphocholine cytidylyltransferase alpha during the S phase
spellingShingle Specific interaction between E2F1 and Sp1 regulates the expression of murine CTP:phosphocholine cytidylyltransferase alpha during the S phase
Elena, Claudia Edicht
cell cycle
proliferation
phospholipids
title_short Specific interaction between E2F1 and Sp1 regulates the expression of murine CTP:phosphocholine cytidylyltransferase alpha during the S phase
title_full Specific interaction between E2F1 and Sp1 regulates the expression of murine CTP:phosphocholine cytidylyltransferase alpha during the S phase
title_fullStr Specific interaction between E2F1 and Sp1 regulates the expression of murine CTP:phosphocholine cytidylyltransferase alpha during the S phase
title_full_unstemmed Specific interaction between E2F1 and Sp1 regulates the expression of murine CTP:phosphocholine cytidylyltransferase alpha during the S phase
title_sort Specific interaction between E2F1 and Sp1 regulates the expression of murine CTP:phosphocholine cytidylyltransferase alpha during the S phase
dc.creator.none.fl_str_mv Elena, Claudia Edicht
Banchio, Claudia Elena
author Elena, Claudia Edicht
author_facet Elena, Claudia Edicht
Banchio, Claudia Elena
author_role author
author2 Banchio, Claudia Elena
author2_role author
dc.subject.none.fl_str_mv cell cycle
proliferation
phospholipids
topic cell cycle
proliferation
phospholipids
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv CTP:phosphocholine cytidylyltransferase alpha (CCTα) is a key enzyme for phosphatidylcholine biosynthesis in mammalian cells. This enzyme plays an essential role in all processes that require membrane biosynthesis such as cell proliferation and viability. Thus, CCTα activity and expression fluctuate during the cell cycle to achieve PtdCho requirements. We demonstrated, for the first time, that CCTα is localized in the nucleus in cells transiting the S phase, whereas it is localized in the cytoplasm of G0-arrested cells, suggesting a specific role of nuclear CCTα during the S phase. We also investigated how E2F1 influences the regulation of the CCTα-promoter during the S phase; we demonstrated that E2F1 is necessary, but not sufficient, to activate CCTα expression when this factor is over-expressed. However, when E2F1 and Sp1 were over-expressed, the transcription from the CCTα-promoter reporter construct was super-activated. Transient transfection studies demonstrated that E2F1 could superactivate Sp1-dependent transcription in a promoter containing only the Sp1 binding sites “B” or “C”, and that Sp1 could activate Sp1-dependent transcription in a promoter containing the E2F site, thus, further demonstrating a functional interaction of these factors. In conclusion, the present results allowed us to portray the clearest picture of the CCTα-gene expression in proliferating cells, and understand the mechanism by which cells coordinate cell cycle progression with the requirement for phosphatidylcholine.
Fil: Elena, Claudia Edicht. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Banchio, Claudia Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
description CTP:phosphocholine cytidylyltransferase alpha (CCTα) is a key enzyme for phosphatidylcholine biosynthesis in mammalian cells. This enzyme plays an essential role in all processes that require membrane biosynthesis such as cell proliferation and viability. Thus, CCTα activity and expression fluctuate during the cell cycle to achieve PtdCho requirements. We demonstrated, for the first time, that CCTα is localized in the nucleus in cells transiting the S phase, whereas it is localized in the cytoplasm of G0-arrested cells, suggesting a specific role of nuclear CCTα during the S phase. We also investigated how E2F1 influences the regulation of the CCTα-promoter during the S phase; we demonstrated that E2F1 is necessary, but not sufficient, to activate CCTα expression when this factor is over-expressed. However, when E2F1 and Sp1 were over-expressed, the transcription from the CCTα-promoter reporter construct was super-activated. Transient transfection studies demonstrated that E2F1 could superactivate Sp1-dependent transcription in a promoter containing only the Sp1 binding sites “B” or “C”, and that Sp1 could activate Sp1-dependent transcription in a promoter containing the E2F site, thus, further demonstrating a functional interaction of these factors. In conclusion, the present results allowed us to portray the clearest picture of the CCTα-gene expression in proliferating cells, and understand the mechanism by which cells coordinate cell cycle progression with the requirement for phosphatidylcholine.
publishDate 2010
dc.date.none.fl_str_mv 2010-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/284288
Elena, Claudia Edicht; Banchio, Claudia Elena; Specific interaction between E2F1 and Sp1 regulates the expression of murine CTP:phosphocholine cytidylyltransferase alpha during the S phase; Elsevier Science; Biochimica Et Biophysica Acta - Molecular and Cell Biology of Lipids; 1801; 4; 4-2010; 537-546
1388-1981
CONICET Digital
CONICET
url http://hdl.handle.net/11336/284288
identifier_str_mv Elena, Claudia Edicht; Banchio, Claudia Elena; Specific interaction between E2F1 and Sp1 regulates the expression of murine CTP:phosphocholine cytidylyltransferase alpha during the S phase; Elsevier Science; Biochimica Et Biophysica Acta - Molecular and Cell Biology of Lipids; 1801; 4; 4-2010; 537-546
1388-1981
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S1388198110000065
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bbalip.2010.01.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.265058

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