Specificity Evaluation of a Polyprotein‐Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species

Autores
Moyano, Roberto Damian; Colombatti Olivieri, María Alejandra; Basconez González, M.; Garrido, J. M.; Juste, R. A.; Alonso, Maria Natalia
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Paratuberculosis (PTB) is a disease affecting ruminant animals. The etiological agent, Mycobacterium avium subspecies PTB (Map), is a short, Gram-positive, acid-fast bacillus. Due to the nature of PTB, diagnosis often occurs at advanced stages of the disease, following the onset of clinical symptoms and prior dissemination of the agent. The specificity of current humoral diagnostic techniques, such as ELISA, is limited, mainly due to the presence of various species of mycobacteria in the environment and other closely related pathogenic mycobacteria that can interfere with the accurate diagnosis of PTB due to the high rate of gene conservation within the genus. In the present study, we evaluated the effectiveness and specificity of an ELISA based on a Map polyprotein for diagnosing bovine PTB. The efficacy of the polyprotein-based ELISA was assessed using serum samples from healthy, Map-infected, and Mycobacterium bovis (MB)-infected cattle. Specificity was further evaluated using serum samples from healthy and MB-infected wild animals. Our findings revealed that in most cases, the degree of cross-reactivity was negligible or low, particularly in cattle, swine, and goats, while it ranged between 10% and 12.5% in sheep and cervids, respectively. These results suggest that the Map polyprotein used in the ELISA represents a valuable tool for the specific diagnosis of PTB in herds.
Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Colombatti Olivieri, María Alejandra. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Basconez González, M.. Centro de Investigación. Neiker - Tecnalia; España
Fil: Garrido, J. M.. Centro de Investigación. Neiker - Tecnalia; España
Fil: Juste, R. A.. Centro de Investigación. Neiker - Tecnalia; España
Fil: Alonso, Maria Natalia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Materia
bovine tuberculosis
cross-reactivity
ELISA
paratuberculosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/285122
Acceder
id CONICETDig_5a3960eb4521f18266fe6086d2450b76
oai_identifier_str oai:ri.conicet.gov.ar:11336/285122
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Specificity Evaluation of a Polyprotein‐Based ELISA Designed for the Detection of Paratuberculosis in Multiple SpeciesMoyano, Roberto DamianColombatti Olivieri, María AlejandraBasconez González, M.Garrido, J. M.Juste, R. A.Alonso, Maria Nataliabovine tuberculosiscross-reactivityELISAparatuberculosishttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Paratuberculosis (PTB) is a disease affecting ruminant animals. The etiological agent, Mycobacterium avium subspecies PTB (Map), is a short, Gram-positive, acid-fast bacillus. Due to the nature of PTB, diagnosis often occurs at advanced stages of the disease, following the onset of clinical symptoms and prior dissemination of the agent. The specificity of current humoral diagnostic techniques, such as ELISA, is limited, mainly due to the presence of various species of mycobacteria in the environment and other closely related pathogenic mycobacteria that can interfere with the accurate diagnosis of PTB due to the high rate of gene conservation within the genus. In the present study, we evaluated the effectiveness and specificity of an ELISA based on a Map polyprotein for diagnosing bovine PTB. The efficacy of the polyprotein-based ELISA was assessed using serum samples from healthy, Map-infected, and Mycobacterium bovis (MB)-infected cattle. Specificity was further evaluated using serum samples from healthy and MB-infected wild animals. Our findings revealed that in most cases, the degree of cross-reactivity was negligible or low, particularly in cattle, swine, and goats, while it ranged between 10% and 12.5% in sheep and cervids, respectively. These results suggest that the Map polyprotein used in the ELISA represents a valuable tool for the specific diagnosis of PTB in herds.Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Colombatti Olivieri, María Alejandra. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Basconez González, M.. Centro de Investigación. Neiker - Tecnalia; EspañaFil: Garrido, J. M.. Centro de Investigación. Neiker - Tecnalia; EspañaFil: Juste, R. A.. Centro de Investigación. Neiker - Tecnalia; EspañaFil: Alonso, Maria Natalia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaWiley2026-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/285122Moyano, Roberto Damian; Colombatti Olivieri, María Alejandra; Basconez González, M.; Garrido, J. M.; Juste, R. A.; et al.; Specificity Evaluation of a Polyprotein‐Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species; Wiley; Veterinary Medicine International; 2026; 1; 1-2026; 1-72090-81132042-0048CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1155/vmi/7325758info:eu-repo/semantics/altIdentifier/doi/10.1155/vmi/7325758info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:30:57Zoai:ri.conicet.gov.ar:11336/285122instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:30:57.533CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Specificity Evaluation of a Polyprotein‐Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species
title Specificity Evaluation of a Polyprotein‐Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species
spellingShingle Specificity Evaluation of a Polyprotein‐Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species
Moyano, Roberto Damian
bovine tuberculosis
cross-reactivity
ELISA
paratuberculosis
title_short Specificity Evaluation of a Polyprotein‐Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species
title_full Specificity Evaluation of a Polyprotein‐Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species
title_fullStr Specificity Evaluation of a Polyprotein‐Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species
title_full_unstemmed Specificity Evaluation of a Polyprotein‐Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species
title_sort Specificity Evaluation of a Polyprotein‐Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species
dc.creator.none.fl_str_mv Moyano, Roberto Damian
Colombatti Olivieri, María Alejandra
Basconez González, M.
Garrido, J. M.
Juste, R. A.
Alonso, Maria Natalia
author Moyano, Roberto Damian
author_facet Moyano, Roberto Damian
Colombatti Olivieri, María Alejandra
Basconez González, M.
Garrido, J. M.
Juste, R. A.
Alonso, Maria Natalia
author_role author
author2 Colombatti Olivieri, María Alejandra
Basconez González, M.
Garrido, J. M.
Juste, R. A.
Alonso, Maria Natalia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv bovine tuberculosis
cross-reactivity
ELISA
paratuberculosis
topic bovine tuberculosis
cross-reactivity
ELISA
paratuberculosis
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Paratuberculosis (PTB) is a disease affecting ruminant animals. The etiological agent, Mycobacterium avium subspecies PTB (Map), is a short, Gram-positive, acid-fast bacillus. Due to the nature of PTB, diagnosis often occurs at advanced stages of the disease, following the onset of clinical symptoms and prior dissemination of the agent. The specificity of current humoral diagnostic techniques, such as ELISA, is limited, mainly due to the presence of various species of mycobacteria in the environment and other closely related pathogenic mycobacteria that can interfere with the accurate diagnosis of PTB due to the high rate of gene conservation within the genus. In the present study, we evaluated the effectiveness and specificity of an ELISA based on a Map polyprotein for diagnosing bovine PTB. The efficacy of the polyprotein-based ELISA was assessed using serum samples from healthy, Map-infected, and Mycobacterium bovis (MB)-infected cattle. Specificity was further evaluated using serum samples from healthy and MB-infected wild animals. Our findings revealed that in most cases, the degree of cross-reactivity was negligible or low, particularly in cattle, swine, and goats, while it ranged between 10% and 12.5% in sheep and cervids, respectively. These results suggest that the Map polyprotein used in the ELISA represents a valuable tool for the specific diagnosis of PTB in herds.
Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Colombatti Olivieri, María Alejandra. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Basconez González, M.. Centro de Investigación. Neiker - Tecnalia; España
Fil: Garrido, J. M.. Centro de Investigación. Neiker - Tecnalia; España
Fil: Juste, R. A.. Centro de Investigación. Neiker - Tecnalia; España
Fil: Alonso, Maria Natalia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
description Paratuberculosis (PTB) is a disease affecting ruminant animals. The etiological agent, Mycobacterium avium subspecies PTB (Map), is a short, Gram-positive, acid-fast bacillus. Due to the nature of PTB, diagnosis often occurs at advanced stages of the disease, following the onset of clinical symptoms and prior dissemination of the agent. The specificity of current humoral diagnostic techniques, such as ELISA, is limited, mainly due to the presence of various species of mycobacteria in the environment and other closely related pathogenic mycobacteria that can interfere with the accurate diagnosis of PTB due to the high rate of gene conservation within the genus. In the present study, we evaluated the effectiveness and specificity of an ELISA based on a Map polyprotein for diagnosing bovine PTB. The efficacy of the polyprotein-based ELISA was assessed using serum samples from healthy, Map-infected, and Mycobacterium bovis (MB)-infected cattle. Specificity was further evaluated using serum samples from healthy and MB-infected wild animals. Our findings revealed that in most cases, the degree of cross-reactivity was negligible or low, particularly in cattle, swine, and goats, while it ranged between 10% and 12.5% in sheep and cervids, respectively. These results suggest that the Map polyprotein used in the ELISA represents a valuable tool for the specific diagnosis of PTB in herds.
publishDate 2026
dc.date.none.fl_str_mv 2026-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/285122
Moyano, Roberto Damian; Colombatti Olivieri, María Alejandra; Basconez González, M.; Garrido, J. M.; Juste, R. A.; et al.; Specificity Evaluation of a Polyprotein‐Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species; Wiley; Veterinary Medicine International; 2026; 1; 1-2026; 1-7
2090-8113
2042-0048
CONICET Digital
CONICET
url http://hdl.handle.net/11336/285122
identifier_str_mv Moyano, Roberto Damian; Colombatti Olivieri, María Alejandra; Basconez González, M.; Garrido, J. M.; Juste, R. A.; et al.; Specificity Evaluation of a Polyprotein‐Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species; Wiley; Veterinary Medicine International; 2026; 1; 1-2026; 1-7
2090-8113
2042-0048
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1155/vmi/7325758
info:eu-repo/semantics/altIdentifier/doi/10.1155/vmi/7325758
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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