Whey as an alternative nutrient medium for growth of sporosarcina pasteurii and its effect on CaCO3 polymorphism and fly ash bioconsolidation

Autores
Chaparro, Sandra; Rojas, Hugo A.; Caicedo, Gerardo; Romanelli, Gustavo Pablo; Pineda Ramos, Pedro Antonio; Luque, Rafael; Martínez, José J.
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Whey in large quantities can cause environmental problems when discarded, because it reduces dissolved oxygen and aquatic life. Nonetheless, it could be used as an easily available and economical alternative to reduce culture medium costs in microbially induced calcium carbonate precipitation (MICP). In this work, a native Sporosarcina pasteurii was isolated and then cultured by using different proportions of whey (W) in nutrient broth (NB). The solids were characterized by XRD, FT-IR, TGA, and SEM. The potential applications in bioconsolidation were also studied. Whey concentration was directly related to CaCO3 production. Higher whey concentrations reduced calcium carbonate purity to nearly 80%. All experiments showed calcite and vaterite fractions, where a whey increment in the media increased calcite content and decreased vaterite content, causing a decrease in crystal size. MICP improved compressive strength (CS) in sand and fly ash. The best CS results were obtained by fly ash treated with 25 W-75 NB (37.2 kPa) and sand with 75 W-25 NB (32.1 kPa). Whey changed crystal polymorphism in biogenic CaCO3 production. Material bioconsolidation depends on the CaCO3 polymorph, thus fly ash was effectively bioconsolidated by crystallization of vaterite and sand by crystallization of calcite.
Fil: Chaparro, Sandra. Universidad Nacional de Colombia; Colombia
Fil: Rojas, Hugo A.. Universidad Nacional de Colombia; Colombia
Fil: Caicedo, Gerardo. Universidad Nacional de Colombia; Colombia
Fil: Romanelli, Gustavo Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; Argentina
Fil: Pineda Ramos, Pedro Antonio. Universidad de Córdoba; España
Fil: Luque, Rafael. Universidad de Córdoba; España
Fil: Martínez, José J.. Universidad Nacional de Colombia; Colombia
Materia
BIOGENIC CACO3
POLYMORPHISM
UREOLYTIC BACTERIA
WHEY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/164908
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/164908
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Whey as an alternative nutrient medium for growth of sporosarcina pasteurii and its effect on CaCO3 polymorphism and fly ash bioconsolidationChaparro, SandraRojas, Hugo A.Caicedo, GerardoRomanelli, Gustavo PabloPineda Ramos, Pedro AntonioLuque, RafaelMartínez, José J.BIOGENIC CACO3POLYMORPHISMUREOLYTIC BACTERIAWHEYhttps://purl.org/becyt/ford/2.4https://purl.org/becyt/ford/2Whey in large quantities can cause environmental problems when discarded, because it reduces dissolved oxygen and aquatic life. Nonetheless, it could be used as an easily available and economical alternative to reduce culture medium costs in microbially induced calcium carbonate precipitation (MICP). In this work, a native Sporosarcina pasteurii was isolated and then cultured by using different proportions of whey (W) in nutrient broth (NB). The solids were characterized by XRD, FT-IR, TGA, and SEM. The potential applications in bioconsolidation were also studied. Whey concentration was directly related to CaCO3 production. Higher whey concentrations reduced calcium carbonate purity to nearly 80%. All experiments showed calcite and vaterite fractions, where a whey increment in the media increased calcite content and decreased vaterite content, causing a decrease in crystal size. MICP improved compressive strength (CS) in sand and fly ash. The best CS results were obtained by fly ash treated with 25 W-75 NB (37.2 kPa) and sand with 75 W-25 NB (32.1 kPa). Whey changed crystal polymorphism in biogenic CaCO3 production. Material bioconsolidation depends on the CaCO3 polymorph, thus fly ash was effectively bioconsolidated by crystallization of vaterite and sand by crystallization of calcite.Fil: Chaparro, Sandra. Universidad Nacional de Colombia; ColombiaFil: Rojas, Hugo A.. Universidad Nacional de Colombia; ColombiaFil: Caicedo, Gerardo. Universidad Nacional de Colombia; ColombiaFil: Romanelli, Gustavo Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; ArgentinaFil: Pineda Ramos, Pedro Antonio. Universidad de Córdoba; EspañaFil: Luque, Rafael. Universidad de Córdoba; EspañaFil: Martínez, José J.. Universidad Nacional de Colombia; ColombiaMDPI AG2021-05-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/164908Chaparro, Sandra; Rojas, Hugo A.; Caicedo, Gerardo; Romanelli, Gustavo Pablo; Pineda Ramos, Pedro Antonio; et al.; Whey as an alternative nutrient medium for growth of sporosarcina pasteurii and its effect on CaCO3 polymorphism and fly ash bioconsolidation; MDPI AG; Materials; 14; 10; 11-5-2021; 1-121996-1944CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/ma14102470info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1996-1944/14/10/2470/htminfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T15:29:26Zoai:ri.conicet.gov.ar:11336/164908instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 15:29:26.868CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Whey as an alternative nutrient medium for growth of sporosarcina pasteurii and its effect on CaCO3 polymorphism and fly ash bioconsolidation
title Whey as an alternative nutrient medium for growth of sporosarcina pasteurii and its effect on CaCO3 polymorphism and fly ash bioconsolidation
spellingShingle Whey as an alternative nutrient medium for growth of sporosarcina pasteurii and its effect on CaCO3 polymorphism and fly ash bioconsolidation
Chaparro, Sandra
BIOGENIC CACO3
POLYMORPHISM
UREOLYTIC BACTERIA
WHEY
title_short Whey as an alternative nutrient medium for growth of sporosarcina pasteurii and its effect on CaCO3 polymorphism and fly ash bioconsolidation
title_full Whey as an alternative nutrient medium for growth of sporosarcina pasteurii and its effect on CaCO3 polymorphism and fly ash bioconsolidation
title_fullStr Whey as an alternative nutrient medium for growth of sporosarcina pasteurii and its effect on CaCO3 polymorphism and fly ash bioconsolidation
title_full_unstemmed Whey as an alternative nutrient medium for growth of sporosarcina pasteurii and its effect on CaCO3 polymorphism and fly ash bioconsolidation
title_sort Whey as an alternative nutrient medium for growth of sporosarcina pasteurii and its effect on CaCO3 polymorphism and fly ash bioconsolidation
dc.creator.none.fl_str_mv Chaparro, Sandra
Rojas, Hugo A.
Caicedo, Gerardo
Romanelli, Gustavo Pablo
Pineda Ramos, Pedro Antonio
Luque, Rafael
Martínez, José J.
author Chaparro, Sandra
author_facet Chaparro, Sandra
Rojas, Hugo A.
Caicedo, Gerardo
Romanelli, Gustavo Pablo
Pineda Ramos, Pedro Antonio
Luque, Rafael
Martínez, José J.
author_role author
author2 Rojas, Hugo A.
Caicedo, Gerardo
Romanelli, Gustavo Pablo
Pineda Ramos, Pedro Antonio
Luque, Rafael
Martínez, José J.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOGENIC CACO3
POLYMORPHISM
UREOLYTIC BACTERIA
WHEY
topic BIOGENIC CACO3
POLYMORPHISM
UREOLYTIC BACTERIA
WHEY
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.4
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Whey in large quantities can cause environmental problems when discarded, because it reduces dissolved oxygen and aquatic life. Nonetheless, it could be used as an easily available and economical alternative to reduce culture medium costs in microbially induced calcium carbonate precipitation (MICP). In this work, a native Sporosarcina pasteurii was isolated and then cultured by using different proportions of whey (W) in nutrient broth (NB). The solids were characterized by XRD, FT-IR, TGA, and SEM. The potential applications in bioconsolidation were also studied. Whey concentration was directly related to CaCO3 production. Higher whey concentrations reduced calcium carbonate purity to nearly 80%. All experiments showed calcite and vaterite fractions, where a whey increment in the media increased calcite content and decreased vaterite content, causing a decrease in crystal size. MICP improved compressive strength (CS) in sand and fly ash. The best CS results were obtained by fly ash treated with 25 W-75 NB (37.2 kPa) and sand with 75 W-25 NB (32.1 kPa). Whey changed crystal polymorphism in biogenic CaCO3 production. Material bioconsolidation depends on the CaCO3 polymorph, thus fly ash was effectively bioconsolidated by crystallization of vaterite and sand by crystallization of calcite.
Fil: Chaparro, Sandra. Universidad Nacional de Colombia; Colombia
Fil: Rojas, Hugo A.. Universidad Nacional de Colombia; Colombia
Fil: Caicedo, Gerardo. Universidad Nacional de Colombia; Colombia
Fil: Romanelli, Gustavo Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; Argentina
Fil: Pineda Ramos, Pedro Antonio. Universidad de Córdoba; España
Fil: Luque, Rafael. Universidad de Córdoba; España
Fil: Martínez, José J.. Universidad Nacional de Colombia; Colombia
description Whey in large quantities can cause environmental problems when discarded, because it reduces dissolved oxygen and aquatic life. Nonetheless, it could be used as an easily available and economical alternative to reduce culture medium costs in microbially induced calcium carbonate precipitation (MICP). In this work, a native Sporosarcina pasteurii was isolated and then cultured by using different proportions of whey (W) in nutrient broth (NB). The solids were characterized by XRD, FT-IR, TGA, and SEM. The potential applications in bioconsolidation were also studied. Whey concentration was directly related to CaCO3 production. Higher whey concentrations reduced calcium carbonate purity to nearly 80%. All experiments showed calcite and vaterite fractions, where a whey increment in the media increased calcite content and decreased vaterite content, causing a decrease in crystal size. MICP improved compressive strength (CS) in sand and fly ash. The best CS results were obtained by fly ash treated with 25 W-75 NB (37.2 kPa) and sand with 75 W-25 NB (32.1 kPa). Whey changed crystal polymorphism in biogenic CaCO3 production. Material bioconsolidation depends on the CaCO3 polymorph, thus fly ash was effectively bioconsolidated by crystallization of vaterite and sand by crystallization of calcite.
publishDate 2021
dc.date.none.fl_str_mv 2021-05-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/164908
Chaparro, Sandra; Rojas, Hugo A.; Caicedo, Gerardo; Romanelli, Gustavo Pablo; Pineda Ramos, Pedro Antonio; et al.; Whey as an alternative nutrient medium for growth of sporosarcina pasteurii and its effect on CaCO3 polymorphism and fly ash bioconsolidation; MDPI AG; Materials; 14; 10; 11-5-2021; 1-12
1996-1944
CONICET Digital
CONICET
url http://hdl.handle.net/11336/164908
identifier_str_mv Chaparro, Sandra; Rojas, Hugo A.; Caicedo, Gerardo; Romanelli, Gustavo Pablo; Pineda Ramos, Pedro Antonio; et al.; Whey as an alternative nutrient medium for growth of sporosarcina pasteurii and its effect on CaCO3 polymorphism and fly ash bioconsolidation; MDPI AG; Materials; 14; 10; 11-5-2021; 1-12
1996-1944
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/ma14102470
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1996-1944/14/10/2470/htm
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.265058

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