Evaluation of performance and matrix compatibility of mixed mode C18-SCX SPME fibers for compounds with different physicochemical properties

Autores
Tascon, Marcos; Boyaci, Ezel; Reyes Garcés, Nathaly; Pawliszyn, Janusz
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Solid-phase microextraction (SPME) has evolved significantly since its inception, yet challenges remain in developing coatings fully compatible with LC-MS that combine broad polarity coverage with biocompatibility for complex matrices. This study evaluates mixed-mode C 18-SCX (strong cation exchange) SPME fibers designed to extract analytes of a wide range of physicochemical properties, addressing limitations in current methodologies. The fibers were tested for extraction efficiency, reproducibility, and matrix compatibility using a group of model compounds with different physicochemical properties, namely, codeine (logP=1.19), carbamazepine (logP=2.45), diazepam (logP=2.82), and propranolol (logP=3.47). Furthermore, the biocompatibility was tested in diverse matrices, such as PBS, blood, plasma, urine, and grape juice. Results demonstrated exceptional inter-fiber reproducibility (RSD ≤ 15 %, n = 96 fibers) and robust performance in biomatrices, with relative matrix effects primarily governed by analyte binding affinities to matrix macromolecules rather than coating fouling. Absolute matrix effects were negligible (93–111 %), underscoring the fibers’ ability to deliver clean extracts for LC-MS analysis. Fiber reusability was validated over three consecutive extractions (RSD ≤ 10 %), and morphological integrity was preserved post-extraction, even in challenging matrices like whole blood. This work represents the versatility of mixed-mode SPME fibers for high- throughput bioanalysis, offering a significant advancement for in vivo and in vitro targeted and untargeted applications.
Fil: Tascon, Marcos. Centro de Estudios Sobre Patrimonio y Ambiente (cepya) ; Escuela de Arte y Patrimonio  ; Universidad Nacional de San Martin; . Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; Argentina. University of Waterloo; Canadá
Fil: Boyaci, Ezel. Middle East Technical University.; Turquía. University of Waterloo; Canadá
Fil: Reyes Garcés, Nathaly. University of Waterloo; Canadá
Fil: Pawliszyn, Janusz. University of Waterloo; Canadá
Materia
SPME
LC-MS
Bioanalysis
Mass spectrometry
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/291963
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/291963
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of performance and matrix compatibility of mixed mode C18-SCX SPME fibers for compounds with different physicochemical propertiesTascon, MarcosBoyaci, EzelReyes Garcés, NathalyPawliszyn, JanuszSPMELC-MSBioanalysisMass spectrometryhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Solid-phase microextraction (SPME) has evolved significantly since its inception, yet challenges remain in developing coatings fully compatible with LC-MS that combine broad polarity coverage with biocompatibility for complex matrices. This study evaluates mixed-mode C 18-SCX (strong cation exchange) SPME fibers designed to extract analytes of a wide range of physicochemical properties, addressing limitations in current methodologies. The fibers were tested for extraction efficiency, reproducibility, and matrix compatibility using a group of model compounds with different physicochemical properties, namely, codeine (logP=1.19), carbamazepine (logP=2.45), diazepam (logP=2.82), and propranolol (logP=3.47). Furthermore, the biocompatibility was tested in diverse matrices, such as PBS, blood, plasma, urine, and grape juice. Results demonstrated exceptional inter-fiber reproducibility (RSD ≤ 15 %, n = 96 fibers) and robust performance in biomatrices, with relative matrix effects primarily governed by analyte binding affinities to matrix macromolecules rather than coating fouling. Absolute matrix effects were negligible (93–111 %), underscoring the fibers’ ability to deliver clean extracts for LC-MS analysis. Fiber reusability was validated over three consecutive extractions (RSD ≤ 10 %), and morphological integrity was preserved post-extraction, even in challenging matrices like whole blood. This work represents the versatility of mixed-mode SPME fibers for high- throughput bioanalysis, offering a significant advancement for in vivo and in vitro targeted and untargeted applications.Fil: Tascon, Marcos. Centro de Estudios Sobre Patrimonio y Ambiente (cepya) ; Escuela de Arte y Patrimonio  ; Universidad Nacional de San Martin; . Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; Argentina. University of Waterloo; CanadáFil: Boyaci, Ezel. Middle East Technical University.; Turquía. University of Waterloo; CanadáFil: Reyes Garcés, Nathaly. University of Waterloo; CanadáFil: Pawliszyn, Janusz. University of Waterloo; CanadáElsevier2025-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/291963Tascon, Marcos; Boyaci, Ezel; Reyes Garcés, Nathaly; Pawliszyn, Janusz; Evaluation of performance and matrix compatibility of mixed mode C18-SCX SPME fibers for compounds with different physicochemical properties; Elsevier; Advances in Sample Preparation; 15; 8-2025; 1-92772-5820CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2772582025000488info:eu-repo/semantics/altIdentifier/doi/10.1016/j.sampre.2025.100195info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T15:37:00Zoai:ri.conicet.gov.ar:11336/291963instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 15:37:00.456CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of performance and matrix compatibility of mixed mode C18-SCX SPME fibers for compounds with different physicochemical properties
title Evaluation of performance and matrix compatibility of mixed mode C18-SCX SPME fibers for compounds with different physicochemical properties
spellingShingle Evaluation of performance and matrix compatibility of mixed mode C18-SCX SPME fibers for compounds with different physicochemical properties
Tascon, Marcos
SPME
LC-MS
Bioanalysis
Mass spectrometry
title_short Evaluation of performance and matrix compatibility of mixed mode C18-SCX SPME fibers for compounds with different physicochemical properties
title_full Evaluation of performance and matrix compatibility of mixed mode C18-SCX SPME fibers for compounds with different physicochemical properties
title_fullStr Evaluation of performance and matrix compatibility of mixed mode C18-SCX SPME fibers for compounds with different physicochemical properties
title_full_unstemmed Evaluation of performance and matrix compatibility of mixed mode C18-SCX SPME fibers for compounds with different physicochemical properties
title_sort Evaluation of performance and matrix compatibility of mixed mode C18-SCX SPME fibers for compounds with different physicochemical properties
dc.creator.none.fl_str_mv Tascon, Marcos
Boyaci, Ezel
Reyes Garcés, Nathaly
Pawliszyn, Janusz
author Tascon, Marcos
author_facet Tascon, Marcos
Boyaci, Ezel
Reyes Garcés, Nathaly
Pawliszyn, Janusz
author_role author
author2 Boyaci, Ezel
Reyes Garcés, Nathaly
Pawliszyn, Janusz
author2_role author
author
author
dc.subject.none.fl_str_mv SPME
LC-MS
Bioanalysis
Mass spectrometry
topic SPME
LC-MS
Bioanalysis
Mass spectrometry
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Solid-phase microextraction (SPME) has evolved significantly since its inception, yet challenges remain in developing coatings fully compatible with LC-MS that combine broad polarity coverage with biocompatibility for complex matrices. This study evaluates mixed-mode C 18-SCX (strong cation exchange) SPME fibers designed to extract analytes of a wide range of physicochemical properties, addressing limitations in current methodologies. The fibers were tested for extraction efficiency, reproducibility, and matrix compatibility using a group of model compounds with different physicochemical properties, namely, codeine (logP=1.19), carbamazepine (logP=2.45), diazepam (logP=2.82), and propranolol (logP=3.47). Furthermore, the biocompatibility was tested in diverse matrices, such as PBS, blood, plasma, urine, and grape juice. Results demonstrated exceptional inter-fiber reproducibility (RSD ≤ 15 %, n = 96 fibers) and robust performance in biomatrices, with relative matrix effects primarily governed by analyte binding affinities to matrix macromolecules rather than coating fouling. Absolute matrix effects were negligible (93–111 %), underscoring the fibers’ ability to deliver clean extracts for LC-MS analysis. Fiber reusability was validated over three consecutive extractions (RSD ≤ 10 %), and morphological integrity was preserved post-extraction, even in challenging matrices like whole blood. This work represents the versatility of mixed-mode SPME fibers for high- throughput bioanalysis, offering a significant advancement for in vivo and in vitro targeted and untargeted applications.
Fil: Tascon, Marcos. Centro de Estudios Sobre Patrimonio y Ambiente (cepya) ; Escuela de Arte y Patrimonio  ; Universidad Nacional de San Martin; . Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; Argentina. University of Waterloo; Canadá
Fil: Boyaci, Ezel. Middle East Technical University.; Turquía. University of Waterloo; Canadá
Fil: Reyes Garcés, Nathaly. University of Waterloo; Canadá
Fil: Pawliszyn, Janusz. University of Waterloo; Canadá
description Solid-phase microextraction (SPME) has evolved significantly since its inception, yet challenges remain in developing coatings fully compatible with LC-MS that combine broad polarity coverage with biocompatibility for complex matrices. This study evaluates mixed-mode C 18-SCX (strong cation exchange) SPME fibers designed to extract analytes of a wide range of physicochemical properties, addressing limitations in current methodologies. The fibers were tested for extraction efficiency, reproducibility, and matrix compatibility using a group of model compounds with different physicochemical properties, namely, codeine (logP=1.19), carbamazepine (logP=2.45), diazepam (logP=2.82), and propranolol (logP=3.47). Furthermore, the biocompatibility was tested in diverse matrices, such as PBS, blood, plasma, urine, and grape juice. Results demonstrated exceptional inter-fiber reproducibility (RSD ≤ 15 %, n = 96 fibers) and robust performance in biomatrices, with relative matrix effects primarily governed by analyte binding affinities to matrix macromolecules rather than coating fouling. Absolute matrix effects were negligible (93–111 %), underscoring the fibers’ ability to deliver clean extracts for LC-MS analysis. Fiber reusability was validated over three consecutive extractions (RSD ≤ 10 %), and morphological integrity was preserved post-extraction, even in challenging matrices like whole blood. This work represents the versatility of mixed-mode SPME fibers for high- throughput bioanalysis, offering a significant advancement for in vivo and in vitro targeted and untargeted applications.
publishDate 2025
dc.date.none.fl_str_mv 2025-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/291963
Tascon, Marcos; Boyaci, Ezel; Reyes Garcés, Nathaly; Pawliszyn, Janusz; Evaluation of performance and matrix compatibility of mixed mode C18-SCX SPME fibers for compounds with different physicochemical properties; Elsevier; Advances in Sample Preparation; 15; 8-2025; 1-9
2772-5820
CONICET Digital
CONICET
url http://hdl.handle.net/11336/291963
identifier_str_mv Tascon, Marcos; Boyaci, Ezel; Reyes Garcés, Nathaly; Pawliszyn, Janusz; Evaluation of performance and matrix compatibility of mixed mode C18-SCX SPME fibers for compounds with different physicochemical properties; Elsevier; Advances in Sample Preparation; 15; 8-2025; 1-9
2772-5820
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2772582025000488
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.sampre.2025.100195
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.265058

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