Bovine sperm capacitation utilizing amino acids and endogenous lipids

Autores
Rodriguez, Pablo; Satorre, María Mercedes; Breininger, Elizabeth; Cetica, Pablo Daniel
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The mechanisms by which spermatozoa generate the energy required for successfulfertilization, as well as the related energy sources, have not been fully elucidated. Weaimed to study the role of amino acids and endogenous lipids as the only oxidativesubstrates in bull sperm capacitation. Sperm samples were incubated in a capacitationmedium with or without classical oxidative substrates. We used L-carnitine andetomoxir, an inducer and an inhibitor of fatty acid ß-oxidation, respectively, to evaluateendogenous lipid consumption and sodium salicylate, an inhibitor of oxidativedeamination, to evaluate exogenous amino acids utilization. We evaluated spermmotility, viability, capacitation, and ammonia production for each treatment. In acapacitation medium without classical oxidative substrates, spermatozoa preservedmotility and viability but failed to undergo capacitation. The addition of L-carnitinesignificantly improved sperm capacitation, while the addition of etomoxir significantlydiminished sperm motility. Ammonia production was significantly increased in thepresence of amino acids, while salicylate counteracted this effect. Sperm capacitationwas observed in media with classical oxidative substrates regardless of the presence ofamino acids. However, capacitation was not detected and motility diminished in mediawith only amino acids added. We demonstrated that catabolism of endogenous lipidscan sustain sperm capacitation as a unique energy source. On the other hand, althoughamino acids can be deaminated by spermatozoa, they cannot be used to sustain spermcapacitation. These findings offer novel insights about energy-dependent processes inbull sperm capacitation and have significant implications for the development ofassisted reproductive technologies.
Fil: Rodriguez, Pablo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Química Biológica; Argentina
Fil: Satorre, María Mercedes. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Química Biológica; Argentina
Fil: Breininger, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Cetica, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Materia
Amino acids
Bovine
Lipids
Sperm capacitation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/291722
Acceder
id CONICETDig_683f26c9926ca411c7a7b43653cfe189
oai_identifier_str oai:ri.conicet.gov.ar:11336/291722
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bovine sperm capacitation utilizing amino acids and endogenous lipidsRodriguez, PabloSatorre, María MercedesBreininger, ElizabethCetica, Pablo DanielAmino acidsBovineLipidsSperm capacitationhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The mechanisms by which spermatozoa generate the energy required for successfulfertilization, as well as the related energy sources, have not been fully elucidated. Weaimed to study the role of amino acids and endogenous lipids as the only oxidativesubstrates in bull sperm capacitation. Sperm samples were incubated in a capacitationmedium with or without classical oxidative substrates. We used L-carnitine andetomoxir, an inducer and an inhibitor of fatty acid ß-oxidation, respectively, to evaluateendogenous lipid consumption and sodium salicylate, an inhibitor of oxidativedeamination, to evaluate exogenous amino acids utilization. We evaluated spermmotility, viability, capacitation, and ammonia production for each treatment. In acapacitation medium without classical oxidative substrates, spermatozoa preservedmotility and viability but failed to undergo capacitation. The addition of L-carnitinesignificantly improved sperm capacitation, while the addition of etomoxir significantlydiminished sperm motility. Ammonia production was significantly increased in thepresence of amino acids, while salicylate counteracted this effect. Sperm capacitationwas observed in media with classical oxidative substrates regardless of the presence ofamino acids. However, capacitation was not detected and motility diminished in mediawith only amino acids added. We demonstrated that catabolism of endogenous lipidscan sustain sperm capacitation as a unique energy source. On the other hand, althoughamino acids can be deaminated by spermatozoa, they cannot be used to sustain spermcapacitation. These findings offer novel insights about energy-dependent processes inbull sperm capacitation and have significant implications for the development ofassisted reproductive technologies.Fil: Rodriguez, Pablo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Química Biológica; ArgentinaFil: Satorre, María Mercedes. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Química Biológica; ArgentinaFil: Breininger, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Cetica, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaUniversidad Nacional Autónoma de México2025-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/291722Rodriguez, Pablo; Satorre, María Mercedes; Breininger, Elizabeth; Cetica, Pablo Daniel; Bovine sperm capacitation utilizing amino acids and endogenous lipids; Universidad Nacional Autónoma de México; Veterinaria Mexico; 12; 9-2025; 1-142448-6760CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://veterinariamexico.fmvz.unam.mx/index.php/vet/article/view/1446info:eu-repo/semantics/altIdentifier/doi/10.22201/fmvz.24486760e.2025.1446info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T15:00:07Zoai:ri.conicet.gov.ar:11336/291722instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 15:00:08.053CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bovine sperm capacitation utilizing amino acids and endogenous lipids
title Bovine sperm capacitation utilizing amino acids and endogenous lipids
spellingShingle Bovine sperm capacitation utilizing amino acids and endogenous lipids
Rodriguez, Pablo
Amino acids
Bovine
Lipids
Sperm capacitation
title_short Bovine sperm capacitation utilizing amino acids and endogenous lipids
title_full Bovine sperm capacitation utilizing amino acids and endogenous lipids
title_fullStr Bovine sperm capacitation utilizing amino acids and endogenous lipids
title_full_unstemmed Bovine sperm capacitation utilizing amino acids and endogenous lipids
title_sort Bovine sperm capacitation utilizing amino acids and endogenous lipids
dc.creator.none.fl_str_mv Rodriguez, Pablo
Satorre, María Mercedes
Breininger, Elizabeth
Cetica, Pablo Daniel
author Rodriguez, Pablo
author_facet Rodriguez, Pablo
Satorre, María Mercedes
Breininger, Elizabeth
Cetica, Pablo Daniel
author_role author
author2 Satorre, María Mercedes
Breininger, Elizabeth
Cetica, Pablo Daniel
author2_role author
author
author
dc.subject.none.fl_str_mv Amino acids
Bovine
Lipids
Sperm capacitation
topic Amino acids
Bovine
Lipids
Sperm capacitation
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The mechanisms by which spermatozoa generate the energy required for successfulfertilization, as well as the related energy sources, have not been fully elucidated. Weaimed to study the role of amino acids and endogenous lipids as the only oxidativesubstrates in bull sperm capacitation. Sperm samples were incubated in a capacitationmedium with or without classical oxidative substrates. We used L-carnitine andetomoxir, an inducer and an inhibitor of fatty acid ß-oxidation, respectively, to evaluateendogenous lipid consumption and sodium salicylate, an inhibitor of oxidativedeamination, to evaluate exogenous amino acids utilization. We evaluated spermmotility, viability, capacitation, and ammonia production for each treatment. In acapacitation medium without classical oxidative substrates, spermatozoa preservedmotility and viability but failed to undergo capacitation. The addition of L-carnitinesignificantly improved sperm capacitation, while the addition of etomoxir significantlydiminished sperm motility. Ammonia production was significantly increased in thepresence of amino acids, while salicylate counteracted this effect. Sperm capacitationwas observed in media with classical oxidative substrates regardless of the presence ofamino acids. However, capacitation was not detected and motility diminished in mediawith only amino acids added. We demonstrated that catabolism of endogenous lipidscan sustain sperm capacitation as a unique energy source. On the other hand, althoughamino acids can be deaminated by spermatozoa, they cannot be used to sustain spermcapacitation. These findings offer novel insights about energy-dependent processes inbull sperm capacitation and have significant implications for the development ofassisted reproductive technologies.
Fil: Rodriguez, Pablo. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Química Biológica; Argentina
Fil: Satorre, María Mercedes. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Área de Química Biológica; Argentina
Fil: Breininger, Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Cetica, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
description The mechanisms by which spermatozoa generate the energy required for successfulfertilization, as well as the related energy sources, have not been fully elucidated. Weaimed to study the role of amino acids and endogenous lipids as the only oxidativesubstrates in bull sperm capacitation. Sperm samples were incubated in a capacitationmedium with or without classical oxidative substrates. We used L-carnitine andetomoxir, an inducer and an inhibitor of fatty acid ß-oxidation, respectively, to evaluateendogenous lipid consumption and sodium salicylate, an inhibitor of oxidativedeamination, to evaluate exogenous amino acids utilization. We evaluated spermmotility, viability, capacitation, and ammonia production for each treatment. In acapacitation medium without classical oxidative substrates, spermatozoa preservedmotility and viability but failed to undergo capacitation. The addition of L-carnitinesignificantly improved sperm capacitation, while the addition of etomoxir significantlydiminished sperm motility. Ammonia production was significantly increased in thepresence of amino acids, while salicylate counteracted this effect. Sperm capacitationwas observed in media with classical oxidative substrates regardless of the presence ofamino acids. However, capacitation was not detected and motility diminished in mediawith only amino acids added. We demonstrated that catabolism of endogenous lipidscan sustain sperm capacitation as a unique energy source. On the other hand, althoughamino acids can be deaminated by spermatozoa, they cannot be used to sustain spermcapacitation. These findings offer novel insights about energy-dependent processes inbull sperm capacitation and have significant implications for the development ofassisted reproductive technologies.
publishDate 2025
dc.date.none.fl_str_mv 2025-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/291722
Rodriguez, Pablo; Satorre, María Mercedes; Breininger, Elizabeth; Cetica, Pablo Daniel; Bovine sperm capacitation utilizing amino acids and endogenous lipids; Universidad Nacional Autónoma de México; Veterinaria Mexico; 12; 9-2025; 1-14
2448-6760
CONICET Digital
CONICET
url http://hdl.handle.net/11336/291722
identifier_str_mv Rodriguez, Pablo; Satorre, María Mercedes; Breininger, Elizabeth; Cetica, Pablo Daniel; Bovine sperm capacitation utilizing amino acids and endogenous lipids; Universidad Nacional Autónoma de México; Veterinaria Mexico; 12; 9-2025; 1-14
2448-6760
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://veterinariamexico.fmvz.unam.mx/index.php/vet/article/view/1446
info:eu-repo/semantics/altIdentifier/doi/10.22201/fmvz.24486760e.2025.1446
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Autónoma de México
publisher.none.fl_str_mv Universidad Nacional Autónoma de México
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24418

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