Complete Genome Classification System of Rotavirus alphagastroenteritidis: An Updated Analysis

Autores
Díaz Alarcón, Ricardo Gabriel; Salvatierra, Karina Alejandra; Gomez Quintero, Emiliano Lautaro; Liotta, Domingo Javier; Parreño, Viviana; Miño, Samuel Orlando
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rotavirus alphagastroenteritidis is the major causative agent of acute gastroenteritis in both children under the age of 5 and young mammals and birds globally. RVAs are non-enveloped viruses with a genome comprising 11 double-stranded RNA segments. In 2008, the Rotavirus Classification Working Group pioneered a comprehensive and complete RVA genome classification system, establishing a specific threshold, which measures the genetic distances between homologous genes. The aim of this study was to perform an updated systematic analysis of the genetic variability across all RVA genes. Our investigation involved assessing the established cutoff values for each RVA genome segment and determining the need for any updates. To achieve this objective, multiple sequence alignments were constructed for all 11 genes and one for each genotype with discrepancies. Also, pairwise distances along with their cutoff values were evaluated. The analyses provided insights into the current relevance of cutoff values, which remain applicable for the majority of genotypes. In conclusion, this study fortifies the current classification system by highlighting its robustness and accurate genotyping of Rotavirus alphagastroenteritidis.
Fil: Díaz Alarcón, Ricardo Gabriel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Salvatierra, Karina Alejandra. Administracion Nacional de Laboratorios E Institutos de Salud "dr. Carlos G. Malbran". Instituto Nacional de Medicina Tropical.; Argentina
Fil: Gomez Quintero, Emiliano Lautaro. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Liotta, Domingo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Miño, Samuel Orlando. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Materia
RVA strain classification
Srain reference
Pairwise distance comparison
Phylogeny
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/284509
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/284509
network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Complete Genome Classification System of Rotavirus alphagastroenteritidis: An Updated AnalysisDíaz Alarcón, Ricardo GabrielSalvatierra, Karina AlejandraGomez Quintero, Emiliano LautaroLiotta, Domingo JavierParreño, VivianaMiño, Samuel OrlandoRVA strain classificationSrain referencePairwise distance comparisonPhylogenyhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Rotavirus alphagastroenteritidis is the major causative agent of acute gastroenteritis in both children under the age of 5 and young mammals and birds globally. RVAs are non-enveloped viruses with a genome comprising 11 double-stranded RNA segments. In 2008, the Rotavirus Classification Working Group pioneered a comprehensive and complete RVA genome classification system, establishing a specific threshold, which measures the genetic distances between homologous genes. The aim of this study was to perform an updated systematic analysis of the genetic variability across all RVA genes. Our investigation involved assessing the established cutoff values for each RVA genome segment and determining the need for any updates. To achieve this objective, multiple sequence alignments were constructed for all 11 genes and one for each genotype with discrepancies. Also, pairwise distances along with their cutoff values were evaluated. The analyses provided insights into the current relevance of cutoff values, which remain applicable for the majority of genotypes. In conclusion, this study fortifies the current classification system by highlighting its robustness and accurate genotyping of Rotavirus alphagastroenteritidis.Fil: Díaz Alarcón, Ricardo Gabriel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Salvatierra, Karina Alejandra. Administracion Nacional de Laboratorios E Institutos de Salud "dr. Carlos G. Malbran". Instituto Nacional de Medicina Tropical.; ArgentinaFil: Gomez Quintero, Emiliano Lautaro. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; ArgentinaFil: Liotta, Domingo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; ArgentinaFil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Miño, Samuel Orlando. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; ArgentinaMDPI2025-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/284509Díaz Alarcón, Ricardo Gabriel; Salvatierra, Karina Alejandra; Gomez Quintero, Emiliano Lautaro; Liotta, Domingo Javier; Parreño, Viviana; et al.; Complete Genome Classification System of Rotavirus alphagastroenteritidis: An Updated Analysis; MDPI; Viruses; 17; 2; 1-2025; 211-2361999-4915CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4915/17/2/211info:eu-repo/semantics/altIdentifier/doi/10.3390/v17020211info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:35:05Zoai:ri.conicet.gov.ar:11336/284509instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:35:05.378CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Complete Genome Classification System of Rotavirus alphagastroenteritidis: An Updated Analysis
title Complete Genome Classification System of Rotavirus alphagastroenteritidis: An Updated Analysis
spellingShingle Complete Genome Classification System of Rotavirus alphagastroenteritidis: An Updated Analysis
Díaz Alarcón, Ricardo Gabriel
RVA strain classification
Srain reference
Pairwise distance comparison
Phylogeny
title_short Complete Genome Classification System of Rotavirus alphagastroenteritidis: An Updated Analysis
title_full Complete Genome Classification System of Rotavirus alphagastroenteritidis: An Updated Analysis
title_fullStr Complete Genome Classification System of Rotavirus alphagastroenteritidis: An Updated Analysis
title_full_unstemmed Complete Genome Classification System of Rotavirus alphagastroenteritidis: An Updated Analysis
title_sort Complete Genome Classification System of Rotavirus alphagastroenteritidis: An Updated Analysis
dc.creator.none.fl_str_mv Díaz Alarcón, Ricardo Gabriel
Salvatierra, Karina Alejandra
Gomez Quintero, Emiliano Lautaro
Liotta, Domingo Javier
Parreño, Viviana
Miño, Samuel Orlando
author Díaz Alarcón, Ricardo Gabriel
author_facet Díaz Alarcón, Ricardo Gabriel
Salvatierra, Karina Alejandra
Gomez Quintero, Emiliano Lautaro
Liotta, Domingo Javier
Parreño, Viviana
Miño, Samuel Orlando
author_role author
author2 Salvatierra, Karina Alejandra
Gomez Quintero, Emiliano Lautaro
Liotta, Domingo Javier
Parreño, Viviana
Miño, Samuel Orlando
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv RVA strain classification
Srain reference
Pairwise distance comparison
Phylogeny
topic RVA strain classification
Srain reference
Pairwise distance comparison
Phylogeny
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Rotavirus alphagastroenteritidis is the major causative agent of acute gastroenteritis in both children under the age of 5 and young mammals and birds globally. RVAs are non-enveloped viruses with a genome comprising 11 double-stranded RNA segments. In 2008, the Rotavirus Classification Working Group pioneered a comprehensive and complete RVA genome classification system, establishing a specific threshold, which measures the genetic distances between homologous genes. The aim of this study was to perform an updated systematic analysis of the genetic variability across all RVA genes. Our investigation involved assessing the established cutoff values for each RVA genome segment and determining the need for any updates. To achieve this objective, multiple sequence alignments were constructed for all 11 genes and one for each genotype with discrepancies. Also, pairwise distances along with their cutoff values were evaluated. The analyses provided insights into the current relevance of cutoff values, which remain applicable for the majority of genotypes. In conclusion, this study fortifies the current classification system by highlighting its robustness and accurate genotyping of Rotavirus alphagastroenteritidis.
Fil: Díaz Alarcón, Ricardo Gabriel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Salvatierra, Karina Alejandra. Administracion Nacional de Laboratorios E Institutos de Salud "dr. Carlos G. Malbran". Instituto Nacional de Medicina Tropical.; Argentina
Fil: Gomez Quintero, Emiliano Lautaro. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Liotta, Domingo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Miño, Samuel Orlando. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentina
description Rotavirus alphagastroenteritidis is the major causative agent of acute gastroenteritis in both children under the age of 5 and young mammals and birds globally. RVAs are non-enveloped viruses with a genome comprising 11 double-stranded RNA segments. In 2008, the Rotavirus Classification Working Group pioneered a comprehensive and complete RVA genome classification system, establishing a specific threshold, which measures the genetic distances between homologous genes. The aim of this study was to perform an updated systematic analysis of the genetic variability across all RVA genes. Our investigation involved assessing the established cutoff values for each RVA genome segment and determining the need for any updates. To achieve this objective, multiple sequence alignments were constructed for all 11 genes and one for each genotype with discrepancies. Also, pairwise distances along with their cutoff values were evaluated. The analyses provided insights into the current relevance of cutoff values, which remain applicable for the majority of genotypes. In conclusion, this study fortifies the current classification system by highlighting its robustness and accurate genotyping of Rotavirus alphagastroenteritidis.
publishDate 2025
dc.date.none.fl_str_mv 2025-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/284509
Díaz Alarcón, Ricardo Gabriel; Salvatierra, Karina Alejandra; Gomez Quintero, Emiliano Lautaro; Liotta, Domingo Javier; Parreño, Viviana; et al.; Complete Genome Classification System of Rotavirus alphagastroenteritidis: An Updated Analysis; MDPI; Viruses; 17; 2; 1-2025; 211-236
1999-4915
CONICET Digital
CONICET
url http://hdl.handle.net/11336/284509
identifier_str_mv Díaz Alarcón, Ricardo Gabriel; Salvatierra, Karina Alejandra; Gomez Quintero, Emiliano Lautaro; Liotta, Domingo Javier; Parreño, Viviana; et al.; Complete Genome Classification System of Rotavirus alphagastroenteritidis: An Updated Analysis; MDPI; Viruses; 17; 2; 1-2025; 211-236
1999-4915
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4915/17/2/211
info:eu-repo/semantics/altIdentifier/doi/10.3390/v17020211
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.265058

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