Shigatoxigenic and Enteropathogenic Escherichia coli Biofilm Formation: Effects of Different Stress Conditions on Polystyrene and Stainless Steel Surfaces

Autores
Cáceres, María Emilia; Etcheverría, Analía Inés; Padola, Nora Lía
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Biofilms constitutes one of the main problems for food industry and publichealth due to the survival and the ability of pathogenic microorganisms to formthese communities on different surfaces and culture composition. Shigatoxigenic(STEC) and Enteropathogenic (EPEC) Escherichia coli are some of themicroorganisms that could form biofilms and cause serious diseases in humans.The aim of this study was to determine the capability to form biofilms of nativeSTEC and atypical EPEC (aEPEC) strains on polystyrene and stainless steel andto evaluate the effects produced by different stress conditions. The expressionof the fimbria curli was also studied. All strains were strong biofilm formers at37°C on polystyrene, except STEC O145:NM and aEPEC O130:H11 which weremoderated biofilm formers. No one strain formed biofilm at heat stress (54°C)or refrigeration (5°C) temperatures. Stainless steel surface reduced significantlythe capability to form biofilms regardless the culture composition. Most of theSTEC and aEPEC strains were curli-negative at 37°C while they showed curlipositivephenotypes at 20°C. Both curli expression and biofilm formation weresignificantly influenced by temperature and incubation time. STEC and aEPECstrains demonstrated to be able to form biofilm and produce fimbria curli underdifferent conditions of medium and temperature, which is important due the riskof survival and transmission of these pathogens from reservoirs and food tohumans.
Fil: Cáceres, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Etcheverría, Analía Inés. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Padola, Nora Lía. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Shigatoxigenic Escherichia coli
atypical enteropathogenic E. coli
BIOFILM
CULTURE
SURFACE
CURLI FIAMBRIAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/151742
Acceder
id CONICETDig_7acbf165de31ecd0e2a76ab3c8895907
oai_identifier_str oai:ri.conicet.gov.ar:11336/151742
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Shigatoxigenic and Enteropathogenic Escherichia coli Biofilm Formation: Effects of Different Stress Conditions on Polystyrene and Stainless Steel SurfacesCáceres, María EmiliaEtcheverría, Analía InésPadola, Nora LíaShigatoxigenic Escherichia coliatypical enteropathogenic E. coliBIOFILMCULTURESURFACECURLI FIAMBRIAEhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Biofilms constitutes one of the main problems for food industry and publichealth due to the survival and the ability of pathogenic microorganisms to formthese communities on different surfaces and culture composition. Shigatoxigenic(STEC) and Enteropathogenic (EPEC) Escherichia coli are some of themicroorganisms that could form biofilms and cause serious diseases in humans.The aim of this study was to determine the capability to form biofilms of nativeSTEC and atypical EPEC (aEPEC) strains on polystyrene and stainless steel andto evaluate the effects produced by different stress conditions. The expressionof the fimbria curli was also studied. All strains were strong biofilm formers at37°C on polystyrene, except STEC O145:NM and aEPEC O130:H11 which weremoderated biofilm formers. No one strain formed biofilm at heat stress (54°C)or refrigeration (5°C) temperatures. Stainless steel surface reduced significantlythe capability to form biofilms regardless the culture composition. Most of theSTEC and aEPEC strains were curli-negative at 37°C while they showed curlipositivephenotypes at 20°C. Both curli expression and biofilm formation weresignificantly influenced by temperature and incubation time. STEC and aEPECstrains demonstrated to be able to form biofilm and produce fimbria curli underdifferent conditions of medium and temperature, which is important due the riskof survival and transmission of these pathogens from reservoirs and food tohumans.Fil: Cáceres, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; ArgentinaFil: Etcheverría, Analía Inés. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Padola, Nora Lía. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAustin Publishing Group2020-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/151742Cáceres, María Emilia; Etcheverría, Analía Inés; Padola, Nora Lía; Shigatoxigenic and Enteropathogenic Escherichia coli Biofilm Formation: Effects of Different Stress Conditions on Polystyrene and Stainless Steel Surfaces; Austin Publishing Group; Journal of Bacteriology and Micology; 7; 8-2020; 1-82471-0172CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://austinpublishinggroup.com/bacteriology/download.php?file=fulltext/bacteriology-v7-id1151.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:44:00Zoai:ri.conicet.gov.ar:11336/151742instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:44:01.238CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Shigatoxigenic and Enteropathogenic Escherichia coli Biofilm Formation: Effects of Different Stress Conditions on Polystyrene and Stainless Steel Surfaces
title Shigatoxigenic and Enteropathogenic Escherichia coli Biofilm Formation: Effects of Different Stress Conditions on Polystyrene and Stainless Steel Surfaces
spellingShingle Shigatoxigenic and Enteropathogenic Escherichia coli Biofilm Formation: Effects of Different Stress Conditions on Polystyrene and Stainless Steel Surfaces
Cáceres, María Emilia
Shigatoxigenic Escherichia coli
atypical enteropathogenic E. coli
BIOFILM
CULTURE
SURFACE
CURLI FIAMBRIAE
title_short Shigatoxigenic and Enteropathogenic Escherichia coli Biofilm Formation: Effects of Different Stress Conditions on Polystyrene and Stainless Steel Surfaces
title_full Shigatoxigenic and Enteropathogenic Escherichia coli Biofilm Formation: Effects of Different Stress Conditions on Polystyrene and Stainless Steel Surfaces
title_fullStr Shigatoxigenic and Enteropathogenic Escherichia coli Biofilm Formation: Effects of Different Stress Conditions on Polystyrene and Stainless Steel Surfaces
title_full_unstemmed Shigatoxigenic and Enteropathogenic Escherichia coli Biofilm Formation: Effects of Different Stress Conditions on Polystyrene and Stainless Steel Surfaces
title_sort Shigatoxigenic and Enteropathogenic Escherichia coli Biofilm Formation: Effects of Different Stress Conditions on Polystyrene and Stainless Steel Surfaces
dc.creator.none.fl_str_mv Cáceres, María Emilia
Etcheverría, Analía Inés
Padola, Nora Lía
author Cáceres, María Emilia
author_facet Cáceres, María Emilia
Etcheverría, Analía Inés
Padola, Nora Lía
author_role author
author2 Etcheverría, Analía Inés
Padola, Nora Lía
author2_role author
author
dc.subject.none.fl_str_mv Shigatoxigenic Escherichia coli
atypical enteropathogenic E. coli
BIOFILM
CULTURE
SURFACE
CURLI FIAMBRIAE
topic Shigatoxigenic Escherichia coli
atypical enteropathogenic E. coli
BIOFILM
CULTURE
SURFACE
CURLI FIAMBRIAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Biofilms constitutes one of the main problems for food industry and publichealth due to the survival and the ability of pathogenic microorganisms to formthese communities on different surfaces and culture composition. Shigatoxigenic(STEC) and Enteropathogenic (EPEC) Escherichia coli are some of themicroorganisms that could form biofilms and cause serious diseases in humans.The aim of this study was to determine the capability to form biofilms of nativeSTEC and atypical EPEC (aEPEC) strains on polystyrene and stainless steel andto evaluate the effects produced by different stress conditions. The expressionof the fimbria curli was also studied. All strains were strong biofilm formers at37°C on polystyrene, except STEC O145:NM and aEPEC O130:H11 which weremoderated biofilm formers. No one strain formed biofilm at heat stress (54°C)or refrigeration (5°C) temperatures. Stainless steel surface reduced significantlythe capability to form biofilms regardless the culture composition. Most of theSTEC and aEPEC strains were curli-negative at 37°C while they showed curlipositivephenotypes at 20°C. Both curli expression and biofilm formation weresignificantly influenced by temperature and incubation time. STEC and aEPECstrains demonstrated to be able to form biofilm and produce fimbria curli underdifferent conditions of medium and temperature, which is important due the riskof survival and transmission of these pathogens from reservoirs and food tohumans.
Fil: Cáceres, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
Fil: Etcheverría, Analía Inés. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Padola, Nora Lía. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Biofilms constitutes one of the main problems for food industry and publichealth due to the survival and the ability of pathogenic microorganisms to formthese communities on different surfaces and culture composition. Shigatoxigenic(STEC) and Enteropathogenic (EPEC) Escherichia coli are some of themicroorganisms that could form biofilms and cause serious diseases in humans.The aim of this study was to determine the capability to form biofilms of nativeSTEC and atypical EPEC (aEPEC) strains on polystyrene and stainless steel andto evaluate the effects produced by different stress conditions. The expressionof the fimbria curli was also studied. All strains were strong biofilm formers at37°C on polystyrene, except STEC O145:NM and aEPEC O130:H11 which weremoderated biofilm formers. No one strain formed biofilm at heat stress (54°C)or refrigeration (5°C) temperatures. Stainless steel surface reduced significantlythe capability to form biofilms regardless the culture composition. Most of theSTEC and aEPEC strains were curli-negative at 37°C while they showed curlipositivephenotypes at 20°C. Both curli expression and biofilm formation weresignificantly influenced by temperature and incubation time. STEC and aEPECstrains demonstrated to be able to form biofilm and produce fimbria curli underdifferent conditions of medium and temperature, which is important due the riskof survival and transmission of these pathogens from reservoirs and food tohumans.
publishDate 2020
dc.date.none.fl_str_mv 2020-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/151742
Cáceres, María Emilia; Etcheverría, Analía Inés; Padola, Nora Lía; Shigatoxigenic and Enteropathogenic Escherichia coli Biofilm Formation: Effects of Different Stress Conditions on Polystyrene and Stainless Steel Surfaces; Austin Publishing Group; Journal of Bacteriology and Micology; 7; 8-2020; 1-8
2471-0172
CONICET Digital
CONICET
url http://hdl.handle.net/11336/151742
identifier_str_mv Cáceres, María Emilia; Etcheverría, Analía Inés; Padola, Nora Lía; Shigatoxigenic and Enteropathogenic Escherichia coli Biofilm Formation: Effects of Different Stress Conditions on Polystyrene and Stainless Steel Surfaces; Austin Publishing Group; Journal of Bacteriology and Micology; 7; 8-2020; 1-8
2471-0172
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://austinpublishinggroup.com/bacteriology/download.php?file=fulltext/bacteriology-v7-id1151.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Austin Publishing Group
publisher.none.fl_str_mv Austin Publishing Group
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.265058

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