Influence of DNA methylation and chromatin accessibility on regulation of gene expression during Trichomonas vaginalis- host cell interaction

Autores
Muñoz, Daniela; Lizarraga, Ayelen; Johnson, Patricia J.; Strobl Mazzulla, Pablo H.; de Miguel, Natalia
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trichomonas vaginalis is a common sexually transmitted parasite that colonizes the human urogenital tract, causing infections that range from asymptomatic to highly inflammatory. As an extracellular pathogen, adherence to host epithelial cells is an important step to colonize the human host. Hence, understanding how the process of attachment to host cells is regulated remains an important goal in parasitology research and human health. T. vaginalis-host interaction is regulated by changes in gene expression, but it is still largely unknown how these changes in transcriptional profilesprofilesprofilesare controlled, as very few transcriptional regulatory elements have been described. Our recent work highlighted the importance of epigenetics in the regulation of transcription, and a specific role for N6-methyladenine (6mA) in modulating three-dimensional chromatin structure has been suggested. Building on these findings, we analyzed here the role of 6mA and chromatin accessibility during the process of host-parasite interaction by integrating MeDIP-seq and assay for transposase-accessible chromatin sequencing data with RNA-seq in free vs host cell-attached parasites. Consistent with our previous results, we identified transcriptionally active and repressive regions flanked by 6mA modifications, observed both in the presence and absence of host cells. Importantly, we detected differentially accessible chromatin regions that influence the gene expression of key pathogenesis-related genes during T. vaginalis host cell interaction. These findings highlight the importance of chromatin architecture in regulating gene expression during parasitic infection.
Fil: Muñoz, Daniela. Universidad Nacional de San Martin. Instituto Tecnologico de Chascomus. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - la Plata. Instituto Tecnologico de Chascomus.; Argentina
Fil: Lizarraga, Ayelen. Universidad Nacional de San Martin. Instituto Tecnologico de Chascomus. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - la Plata. Instituto Tecnologico de Chascomus.; Argentina
Fil: Johnson, Patricia J.. University of California at Los Angeles; Estados Unidos
Fil: Strobl Mazzulla, Pablo H.. Universidad Nacional de San Martin. Instituto Tecnologico de Chascomus. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - la Plata. Instituto Tecnologico de Chascomus.; Argentina
Fil: de Miguel, Natalia. Universidad Nacional de San Martin. Instituto Tecnologico de Chascomus. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - la Plata. Instituto Tecnologico de Chascomus.; Argentina
Materia
Trichomonas vaginalis
DNA methylation
epigenetics
chromatin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/290015
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/290015
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Influence of DNA methylation and chromatin accessibility on regulation of gene expression during Trichomonas vaginalis- host cell interactionMuñoz, DanielaLizarraga, AyelenJohnson, Patricia J.Strobl Mazzulla, Pablo H.de Miguel, NataliaTrichomonas vaginalisDNA methylationepigeneticschromatinhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trichomonas vaginalis is a common sexually transmitted parasite that colonizes the human urogenital tract, causing infections that range from asymptomatic to highly inflammatory. As an extracellular pathogen, adherence to host epithelial cells is an important step to colonize the human host. Hence, understanding how the process of attachment to host cells is regulated remains an important goal in parasitology research and human health. T. vaginalis-host interaction is regulated by changes in gene expression, but it is still largely unknown how these changes in transcriptional profilesprofilesprofilesare controlled, as very few transcriptional regulatory elements have been described. Our recent work highlighted the importance of epigenetics in the regulation of transcription, and a specific role for N6-methyladenine (6mA) in modulating three-dimensional chromatin structure has been suggested. Building on these findings, we analyzed here the role of 6mA and chromatin accessibility during the process of host-parasite interaction by integrating MeDIP-seq and assay for transposase-accessible chromatin sequencing data with RNA-seq in free vs host cell-attached parasites. Consistent with our previous results, we identified transcriptionally active and repressive regions flanked by 6mA modifications, observed both in the presence and absence of host cells. Importantly, we detected differentially accessible chromatin regions that influence the gene expression of key pathogenesis-related genes during T. vaginalis host cell interaction. These findings highlight the importance of chromatin architecture in regulating gene expression during parasitic infection.Fil: Muñoz, Daniela. Universidad Nacional de San Martin. Instituto Tecnologico de Chascomus. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - la Plata. Instituto Tecnologico de Chascomus.; ArgentinaFil: Lizarraga, Ayelen. Universidad Nacional de San Martin. Instituto Tecnologico de Chascomus. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - la Plata. Instituto Tecnologico de Chascomus.; ArgentinaFil: Johnson, Patricia J.. University of California at Los Angeles; Estados UnidosFil: Strobl Mazzulla, Pablo H.. Universidad Nacional de San Martin. Instituto Tecnologico de Chascomus. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - la Plata. Instituto Tecnologico de Chascomus.; ArgentinaFil: de Miguel, Natalia. Universidad Nacional de San Martin. Instituto Tecnologico de Chascomus. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - la Plata. Instituto Tecnologico de Chascomus.; ArgentinaAmerican Society for Microbiology2026-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/290015Muñoz, Daniela; Lizarraga, Ayelen; Johnson, Patricia J.; Strobl Mazzulla, Pablo H.; de Miguel, Natalia; Influence of DNA methylation and chromatin accessibility on regulation of gene expression during Trichomonas vaginalis- host cell interaction; American Society for Microbiology; mBio; 17; 1; 1-2026; 1-212150-7511CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/mbio.03175-25info:eu-repo/semantics/altIdentifier/doi/10.1128/mbio.03175-25info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:32:20Zoai:ri.conicet.gov.ar:11336/290015instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:32:20.418CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Influence of DNA methylation and chromatin accessibility on regulation of gene expression during Trichomonas vaginalis- host cell interaction
title Influence of DNA methylation and chromatin accessibility on regulation of gene expression during Trichomonas vaginalis- host cell interaction
spellingShingle Influence of DNA methylation and chromatin accessibility on regulation of gene expression during Trichomonas vaginalis- host cell interaction
Muñoz, Daniela
Trichomonas vaginalis
DNA methylation
epigenetics
chromatin
title_short Influence of DNA methylation and chromatin accessibility on regulation of gene expression during Trichomonas vaginalis- host cell interaction
title_full Influence of DNA methylation and chromatin accessibility on regulation of gene expression during Trichomonas vaginalis- host cell interaction
title_fullStr Influence of DNA methylation and chromatin accessibility on regulation of gene expression during Trichomonas vaginalis- host cell interaction
title_full_unstemmed Influence of DNA methylation and chromatin accessibility on regulation of gene expression during Trichomonas vaginalis- host cell interaction
title_sort Influence of DNA methylation and chromatin accessibility on regulation of gene expression during Trichomonas vaginalis- host cell interaction
dc.creator.none.fl_str_mv Muñoz, Daniela
Lizarraga, Ayelen
Johnson, Patricia J.
Strobl Mazzulla, Pablo H.
de Miguel, Natalia
author Muñoz, Daniela
author_facet Muñoz, Daniela
Lizarraga, Ayelen
Johnson, Patricia J.
Strobl Mazzulla, Pablo H.
de Miguel, Natalia
author_role author
author2 Lizarraga, Ayelen
Johnson, Patricia J.
Strobl Mazzulla, Pablo H.
de Miguel, Natalia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Trichomonas vaginalis
DNA methylation
epigenetics
chromatin
topic Trichomonas vaginalis
DNA methylation
epigenetics
chromatin
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Trichomonas vaginalis is a common sexually transmitted parasite that colonizes the human urogenital tract, causing infections that range from asymptomatic to highly inflammatory. As an extracellular pathogen, adherence to host epithelial cells is an important step to colonize the human host. Hence, understanding how the process of attachment to host cells is regulated remains an important goal in parasitology research and human health. T. vaginalis-host interaction is regulated by changes in gene expression, but it is still largely unknown how these changes in transcriptional profilesprofilesprofilesare controlled, as very few transcriptional regulatory elements have been described. Our recent work highlighted the importance of epigenetics in the regulation of transcription, and a specific role for N6-methyladenine (6mA) in modulating three-dimensional chromatin structure has been suggested. Building on these findings, we analyzed here the role of 6mA and chromatin accessibility during the process of host-parasite interaction by integrating MeDIP-seq and assay for transposase-accessible chromatin sequencing data with RNA-seq in free vs host cell-attached parasites. Consistent with our previous results, we identified transcriptionally active and repressive regions flanked by 6mA modifications, observed both in the presence and absence of host cells. Importantly, we detected differentially accessible chromatin regions that influence the gene expression of key pathogenesis-related genes during T. vaginalis host cell interaction. These findings highlight the importance of chromatin architecture in regulating gene expression during parasitic infection.
Fil: Muñoz, Daniela. Universidad Nacional de San Martin. Instituto Tecnologico de Chascomus. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - la Plata. Instituto Tecnologico de Chascomus.; Argentina
Fil: Lizarraga, Ayelen. Universidad Nacional de San Martin. Instituto Tecnologico de Chascomus. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - la Plata. Instituto Tecnologico de Chascomus.; Argentina
Fil: Johnson, Patricia J.. University of California at Los Angeles; Estados Unidos
Fil: Strobl Mazzulla, Pablo H.. Universidad Nacional de San Martin. Instituto Tecnologico de Chascomus. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - la Plata. Instituto Tecnologico de Chascomus.; Argentina
Fil: de Miguel, Natalia. Universidad Nacional de San Martin. Instituto Tecnologico de Chascomus. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - la Plata. Instituto Tecnologico de Chascomus.; Argentina
description Trichomonas vaginalis is a common sexually transmitted parasite that colonizes the human urogenital tract, causing infections that range from asymptomatic to highly inflammatory. As an extracellular pathogen, adherence to host epithelial cells is an important step to colonize the human host. Hence, understanding how the process of attachment to host cells is regulated remains an important goal in parasitology research and human health. T. vaginalis-host interaction is regulated by changes in gene expression, but it is still largely unknown how these changes in transcriptional profilesprofilesprofilesare controlled, as very few transcriptional regulatory elements have been described. Our recent work highlighted the importance of epigenetics in the regulation of transcription, and a specific role for N6-methyladenine (6mA) in modulating three-dimensional chromatin structure has been suggested. Building on these findings, we analyzed here the role of 6mA and chromatin accessibility during the process of host-parasite interaction by integrating MeDIP-seq and assay for transposase-accessible chromatin sequencing data with RNA-seq in free vs host cell-attached parasites. Consistent with our previous results, we identified transcriptionally active and repressive regions flanked by 6mA modifications, observed both in the presence and absence of host cells. Importantly, we detected differentially accessible chromatin regions that influence the gene expression of key pathogenesis-related genes during T. vaginalis host cell interaction. These findings highlight the importance of chromatin architecture in regulating gene expression during parasitic infection.
publishDate 2026
dc.date.none.fl_str_mv 2026-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/290015
Muñoz, Daniela; Lizarraga, Ayelen; Johnson, Patricia J.; Strobl Mazzulla, Pablo H.; de Miguel, Natalia; Influence of DNA methylation and chromatin accessibility on regulation of gene expression during Trichomonas vaginalis- host cell interaction; American Society for Microbiology; mBio; 17; 1; 1-2026; 1-21
2150-7511
CONICET Digital
CONICET
url http://hdl.handle.net/11336/290015
identifier_str_mv Muñoz, Daniela; Lizarraga, Ayelen; Johnson, Patricia J.; Strobl Mazzulla, Pablo H.; de Miguel, Natalia; Influence of DNA methylation and chromatin accessibility on regulation of gene expression during Trichomonas vaginalis- host cell interaction; American Society for Microbiology; mBio; 17; 1; 1-2026; 1-21
2150-7511
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.asm.org/doi/10.1128/mbio.03175-25
info:eu-repo/semantics/altIdentifier/doi/10.1128/mbio.03175-25
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.265058

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