Crotalus Durissus Terrificus venom ad- sorbed onto silica nanoparticles for potential use in serotherapy

Autores
Conti, Florencia Silvina; Giorgi, Exequiel David; Rodríguez, Juan Pablo; Baudou, Federico Gastón; de Marzi, Mauricio Cesar
Año de publicación
2025
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Ophidism is a globally neglected health problem. In Argentina, Cro-talus durissus terrificus (C.d.t.) is one of the species of greater med-ical importance since its venom (V) contains mainly crotoxin (CTX),a potent toxin responsible for its high lethality. The generation of an-tivenoms (AVs) is a costly and difficult task, and the possibility of us-ing Silica nanoparticles (SiNPs) transporting snake toxins (complexcalled nanovenom-NV-) appears as a new tool to produce them. Inthis work, we study some physicochemical properties of NV complexformed by C.d.t. V and SiNPs with diameters of 150 nm and 330 nm.Proper C.d.t V adsorption to both sizes of Stber SiNPs was con-firmed by TEM micrographs. The concentration of adsorbed V wasdetermined using UV-visible spectrometry by measuring absor-bance at 280 nm. As a result, more than 50% of different concen-trations of V (0.025 – 2 mg/ml) were adsorbed to the SiNPs. Ad-sorption isotherms were analyzed using Langmuir and Freundlichmodels to explain different forms of adsorption-NPs interaction. Itwas observed that the 150 nm NVs fit both models well (R2 0.98)whether 330 nm NVs presented a lower R2 (0.88 for Freundlich and0.87 for Langmuir). These results suggest that the proteins of theV assume the formation of multiple layers due to a heterogeneous surface with regions of high and low affinity. These findings demonstrate that SiNPs can effectively adsorb C.d.t. venom and may actas promising platforms for controlled antigen delivery. This approachopens the possibility of improving antivenom production strategies.
Fil: Conti, Florencia Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Giorgi, Exequiel David. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Rodríguez, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Baudou, Federico Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: de Marzi, Mauricio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; Argentina
XX Reunión Anual de La Sociedad Argentina de Investigación Clínica y LVII Reunión Anual de La Asociación Argentina de Farmacología Experimental
Buenos Aires
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
Materia
Ofidismo
Encapsulados
PLA2
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/291192
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/291192
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Crotalus Durissus Terrificus venom ad- sorbed onto silica nanoparticles for potential use in serotherapyConti, Florencia SilvinaGiorgi, Exequiel DavidRodríguez, Juan PabloBaudou, Federico Gastónde Marzi, Mauricio CesarOfidismoEncapsuladosPLA2https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Ophidism is a globally neglected health problem. In Argentina, Cro-talus durissus terrificus (C.d.t.) is one of the species of greater med-ical importance since its venom (V) contains mainly crotoxin (CTX),a potent toxin responsible for its high lethality. The generation of an-tivenoms (AVs) is a costly and difficult task, and the possibility of us-ing Silica nanoparticles (SiNPs) transporting snake toxins (complexcalled nanovenom-NV-) appears as a new tool to produce them. Inthis work, we study some physicochemical properties of NV complexformed by C.d.t. V and SiNPs with diameters of 150 nm and 330 nm.Proper C.d.t V adsorption to both sizes of Stber SiNPs was con-firmed by TEM micrographs. The concentration of adsorbed V wasdetermined using UV-visible spectrometry by measuring absor-bance at 280 nm. As a result, more than 50% of different concen-trations of V (0.025 – 2 mg/ml) were adsorbed to the SiNPs. Ad-sorption isotherms were analyzed using Langmuir and Freundlichmodels to explain different forms of adsorption-NPs interaction. Itwas observed that the 150 nm NVs fit both models well (R2 0.98)whether 330 nm NVs presented a lower R2 (0.88 for Freundlich and0.87 for Langmuir). These results suggest that the proteins of theV assume the formation of multiple layers due to a heterogeneous surface with regions of high and low affinity. These findings demonstrate that SiNPs can effectively adsorb C.d.t. venom and may actas promising platforms for controlled antigen delivery. This approachopens the possibility of improving antivenom production strategies.Fil: Conti, Florencia Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; ArgentinaFil: Giorgi, Exequiel David. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; ArgentinaFil: Rodríguez, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Baudou, Federico Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; ArgentinaFil: de Marzi, Mauricio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; ArgentinaXX Reunión Anual de La Sociedad Argentina de Investigación Clínica y LVII Reunión Anual de La Asociación Argentina de Farmacología ExperimentalBuenos AiresArgentinaSociedad Argentina de Investigación ClínicaAsociación Argentina de Farmacología ExperimentalFundación Revista Medicina2025info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/291192Crotalus Durissus Terrificus venom ad- sorbed onto silica nanoparticles for potential use in serotherapy; XX Reunión Anual de La Sociedad Argentina de Investigación Clínica y LVII Reunión Anual de La Asociación Argentina de Farmacología Experimental; Buenos Aires; Argentina; 2025; 66-671669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saic.org.ar/revista-medicina/medicina-2025/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T15:02:36Zoai:ri.conicet.gov.ar:11336/291192instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 15:02:36.321CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Crotalus Durissus Terrificus venom ad- sorbed onto silica nanoparticles for potential use in serotherapy
title Crotalus Durissus Terrificus venom ad- sorbed onto silica nanoparticles for potential use in serotherapy
spellingShingle Crotalus Durissus Terrificus venom ad- sorbed onto silica nanoparticles for potential use in serotherapy
Conti, Florencia Silvina
Ofidismo
Encapsulados
PLA2
title_short Crotalus Durissus Terrificus venom ad- sorbed onto silica nanoparticles for potential use in serotherapy
title_full Crotalus Durissus Terrificus venom ad- sorbed onto silica nanoparticles for potential use in serotherapy
title_fullStr Crotalus Durissus Terrificus venom ad- sorbed onto silica nanoparticles for potential use in serotherapy
title_full_unstemmed Crotalus Durissus Terrificus venom ad- sorbed onto silica nanoparticles for potential use in serotherapy
title_sort Crotalus Durissus Terrificus venom ad- sorbed onto silica nanoparticles for potential use in serotherapy
dc.creator.none.fl_str_mv Conti, Florencia Silvina
Giorgi, Exequiel David
Rodríguez, Juan Pablo
Baudou, Federico Gastón
de Marzi, Mauricio Cesar
author Conti, Florencia Silvina
author_facet Conti, Florencia Silvina
Giorgi, Exequiel David
Rodríguez, Juan Pablo
Baudou, Federico Gastón
de Marzi, Mauricio Cesar
author_role author
author2 Giorgi, Exequiel David
Rodríguez, Juan Pablo
Baudou, Federico Gastón
de Marzi, Mauricio Cesar
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ofidismo
Encapsulados
PLA2
topic Ofidismo
Encapsulados
PLA2
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Ophidism is a globally neglected health problem. In Argentina, Cro-talus durissus terrificus (C.d.t.) is one of the species of greater med-ical importance since its venom (V) contains mainly crotoxin (CTX),a potent toxin responsible for its high lethality. The generation of an-tivenoms (AVs) is a costly and difficult task, and the possibility of us-ing Silica nanoparticles (SiNPs) transporting snake toxins (complexcalled nanovenom-NV-) appears as a new tool to produce them. Inthis work, we study some physicochemical properties of NV complexformed by C.d.t. V and SiNPs with diameters of 150 nm and 330 nm.Proper C.d.t V adsorption to both sizes of Stber SiNPs was con-firmed by TEM micrographs. The concentration of adsorbed V wasdetermined using UV-visible spectrometry by measuring absor-bance at 280 nm. As a result, more than 50% of different concen-trations of V (0.025 – 2 mg/ml) were adsorbed to the SiNPs. Ad-sorption isotherms were analyzed using Langmuir and Freundlichmodels to explain different forms of adsorption-NPs interaction. Itwas observed that the 150 nm NVs fit both models well (R2 0.98)whether 330 nm NVs presented a lower R2 (0.88 for Freundlich and0.87 for Langmuir). These results suggest that the proteins of theV assume the formation of multiple layers due to a heterogeneous surface with regions of high and low affinity. These findings demonstrate that SiNPs can effectively adsorb C.d.t. venom and may actas promising platforms for controlled antigen delivery. This approachopens the possibility of improving antivenom production strategies.
Fil: Conti, Florencia Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Giorgi, Exequiel David. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Rodríguez, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Baudou, Federico Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: de Marzi, Mauricio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; Argentina
XX Reunión Anual de La Sociedad Argentina de Investigación Clínica y LVII Reunión Anual de La Asociación Argentina de Farmacología Experimental
Buenos Aires
Argentina
Sociedad Argentina de Investigación Clínica
Asociación Argentina de Farmacología Experimental
description Ophidism is a globally neglected health problem. In Argentina, Cro-talus durissus terrificus (C.d.t.) is one of the species of greater med-ical importance since its venom (V) contains mainly crotoxin (CTX),a potent toxin responsible for its high lethality. The generation of an-tivenoms (AVs) is a costly and difficult task, and the possibility of us-ing Silica nanoparticles (SiNPs) transporting snake toxins (complexcalled nanovenom-NV-) appears as a new tool to produce them. Inthis work, we study some physicochemical properties of NV complexformed by C.d.t. V and SiNPs with diameters of 150 nm and 330 nm.Proper C.d.t V adsorption to both sizes of Stber SiNPs was con-firmed by TEM micrographs. The concentration of adsorbed V wasdetermined using UV-visible spectrometry by measuring absor-bance at 280 nm. As a result, more than 50% of different concen-trations of V (0.025 – 2 mg/ml) were adsorbed to the SiNPs. Ad-sorption isotherms were analyzed using Langmuir and Freundlichmodels to explain different forms of adsorption-NPs interaction. Itwas observed that the 150 nm NVs fit both models well (R2 0.98)whether 330 nm NVs presented a lower R2 (0.88 for Freundlich and0.87 for Langmuir). These results suggest that the proteins of theV assume the formation of multiple layers due to a heterogeneous surface with regions of high and low affinity. These findings demonstrate that SiNPs can effectively adsorb C.d.t. venom and may actas promising platforms for controlled antigen delivery. This approachopens the possibility of improving antivenom production strategies.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/291192
Crotalus Durissus Terrificus venom ad- sorbed onto silica nanoparticles for potential use in serotherapy; XX Reunión Anual de La Sociedad Argentina de Investigación Clínica y LVII Reunión Anual de La Asociación Argentina de Farmacología Experimental; Buenos Aires; Argentina; 2025; 66-67
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/291192
identifier_str_mv Crotalus Durissus Terrificus venom ad- sorbed onto silica nanoparticles for potential use in serotherapy; XX Reunión Anual de La Sociedad Argentina de Investigación Clínica y LVII Reunión Anual de La Asociación Argentina de Farmacología Experimental; Buenos Aires; Argentina; 2025; 66-67
1669-9106
CONICET Digital
CONICET
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language eng
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rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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