Electrochemical monitoring of ethinylestradiol oxidation by the horseradish peroxidase enzyme in water

Autores
Scala Benuzzi, María Luz; Martinez, Noelia Anabel; Pereira, Sirley Vanesa; Raba, Julio; Messina, Germán Alejandro
Año de publicación
2014
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Ethinylestradiol (EE2) is a synthetic estrogen used in contraceptive therapy. The presence of this compound in the environment has been attributed to their incomplete removal in wastewater treatment plant processes. The purpose of this research was to optimize the Horseradish peroxidase (HRP) enzyme-catalyzed process for removal of EE2 from synthetic water (purified deionized water) and the monitoring of the enzymatic process by square-wave voltammetry (SWV). In this study, HRP catalyzes the oxidation of EE2 in presence of H2O2 and the products formed are polymerized through a non-enzymatic process which leads to the formation of high molecular weight polymers. These low-solubility polymers can be removed from wastewater by co-precipitation, sorption to solids, sedimentation or filtration. The reaction mixture was prepared by diluting stock solution of a EE2 to a concentration of 0.5 µM, in 10 mL of 0.01 M citrate buffer pH 5.0 containing HRP 0.02 U mL-1. The reaction was initiated by adding 1 mM of H2O2. Then, after stirring for 10 min, the sample was centrifugated and EE2 was quantified by SWV. The enzymatic process was monitored by SWV using a three electrodes system: glassy carbon working electrode, Ag|AgCl|3M NaCl reference electrode and Pt counter electrode. For the measurement of EE2 by SWV a calibration equation was ΔI (µA) = 1.436 + 2.382 CEE2 with a correlation coefficient of 0.998, over the concentration range of 0.3?50 µM with a limit of detection of 0.11µM. Our analysis demonstrated that EE2 disappeared almost completely in the reaction mixture after 10 min treatment. These results strongly suggest that HRP is effective in removing the estrogenic activities of EE2 in water samples.
Fil: Scala Benuzzi, María Luz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Martinez, Noelia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
3 rd International Meeting on Pharmaceutical Sciences
Córdoba
Argentina
Society of Pharmaceutical Sciences and Research
Materia
ELECTROCHEMISTRY
HORSERADISH PEROXIDASE ENZIME
ETHINYLESTRADIOL
ENVIRONMENT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/290206
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/290206
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Electrochemical monitoring of ethinylestradiol oxidation by the horseradish peroxidase enzyme in waterScala Benuzzi, María LuzMartinez, Noelia AnabelPereira, Sirley VanesaRaba, JulioMessina, Germán AlejandroELECTROCHEMISTRYHORSERADISH PEROXIDASE ENZIMEETHINYLESTRADIOLENVIRONMENThttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Ethinylestradiol (EE2) is a synthetic estrogen used in contraceptive therapy. The presence of this compound in the environment has been attributed to their incomplete removal in wastewater treatment plant processes. The purpose of this research was to optimize the Horseradish peroxidase (HRP) enzyme-catalyzed process for removal of EE2 from synthetic water (purified deionized water) and the monitoring of the enzymatic process by square-wave voltammetry (SWV). In this study, HRP catalyzes the oxidation of EE2 in presence of H2O2 and the products formed are polymerized through a non-enzymatic process which leads to the formation of high molecular weight polymers. These low-solubility polymers can be removed from wastewater by co-precipitation, sorption to solids, sedimentation or filtration. The reaction mixture was prepared by diluting stock solution of a EE2 to a concentration of 0.5 µM, in 10 mL of 0.01 M citrate buffer pH 5.0 containing HRP 0.02 U mL-1. The reaction was initiated by adding 1 mM of H2O2. Then, after stirring for 10 min, the sample was centrifugated and EE2 was quantified by SWV. The enzymatic process was monitored by SWV using a three electrodes system: glassy carbon working electrode, Ag|AgCl|3M NaCl reference electrode and Pt counter electrode. For the measurement of EE2 by SWV a calibration equation was ΔI (µA) = 1.436 + 2.382 CEE2 with a correlation coefficient of 0.998, over the concentration range of 0.3?50 µM with a limit of detection of 0.11µM. Our analysis demonstrated that EE2 disappeared almost completely in the reaction mixture after 10 min treatment. These results strongly suggest that HRP is effective in removing the estrogenic activities of EE2 in water samples.Fil: Scala Benuzzi, María Luz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Martinez, Noelia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina3 rd International Meeting on Pharmaceutical SciencesCórdobaArgentinaSociety of Pharmaceutical Sciences and ResearchSociety of Pharmaceutical Sciences and Research2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/290206Electrochemical monitoring of ethinylestradiol oxidation by the horseradish peroxidase enzyme in water; 3 rd International Meeting on Pharmaceutical Sciences; Córdoba; Argentina; 2014; 1-80975-8232CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://ricifa.com.ar/wp-content/uploads/2021/09/Resumenes-2010.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:32:43Zoai:ri.conicet.gov.ar:11336/290206instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:32:44.218CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Electrochemical monitoring of ethinylestradiol oxidation by the horseradish peroxidase enzyme in water
title Electrochemical monitoring of ethinylestradiol oxidation by the horseradish peroxidase enzyme in water
spellingShingle Electrochemical monitoring of ethinylestradiol oxidation by the horseradish peroxidase enzyme in water
Scala Benuzzi, María Luz
ELECTROCHEMISTRY
HORSERADISH PEROXIDASE ENZIME
ETHINYLESTRADIOL
ENVIRONMENT
title_short Electrochemical monitoring of ethinylestradiol oxidation by the horseradish peroxidase enzyme in water
title_full Electrochemical monitoring of ethinylestradiol oxidation by the horseradish peroxidase enzyme in water
title_fullStr Electrochemical monitoring of ethinylestradiol oxidation by the horseradish peroxidase enzyme in water
title_full_unstemmed Electrochemical monitoring of ethinylestradiol oxidation by the horseradish peroxidase enzyme in water
title_sort Electrochemical monitoring of ethinylestradiol oxidation by the horseradish peroxidase enzyme in water
dc.creator.none.fl_str_mv Scala Benuzzi, María Luz
Martinez, Noelia Anabel
Pereira, Sirley Vanesa
Raba, Julio
Messina, Germán Alejandro
author Scala Benuzzi, María Luz
author_facet Scala Benuzzi, María Luz
Martinez, Noelia Anabel
Pereira, Sirley Vanesa
Raba, Julio
Messina, Germán Alejandro
author_role author
author2 Martinez, Noelia Anabel
Pereira, Sirley Vanesa
Raba, Julio
Messina, Germán Alejandro
author2_role author
author
author
author
dc.subject.none.fl_str_mv ELECTROCHEMISTRY
HORSERADISH PEROXIDASE ENZIME
ETHINYLESTRADIOL
ENVIRONMENT
topic ELECTROCHEMISTRY
HORSERADISH PEROXIDASE ENZIME
ETHINYLESTRADIOL
ENVIRONMENT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Ethinylestradiol (EE2) is a synthetic estrogen used in contraceptive therapy. The presence of this compound in the environment has been attributed to their incomplete removal in wastewater treatment plant processes. The purpose of this research was to optimize the Horseradish peroxidase (HRP) enzyme-catalyzed process for removal of EE2 from synthetic water (purified deionized water) and the monitoring of the enzymatic process by square-wave voltammetry (SWV). In this study, HRP catalyzes the oxidation of EE2 in presence of H2O2 and the products formed are polymerized through a non-enzymatic process which leads to the formation of high molecular weight polymers. These low-solubility polymers can be removed from wastewater by co-precipitation, sorption to solids, sedimentation or filtration. The reaction mixture was prepared by diluting stock solution of a EE2 to a concentration of 0.5 µM, in 10 mL of 0.01 M citrate buffer pH 5.0 containing HRP 0.02 U mL-1. The reaction was initiated by adding 1 mM of H2O2. Then, after stirring for 10 min, the sample was centrifugated and EE2 was quantified by SWV. The enzymatic process was monitored by SWV using a three electrodes system: glassy carbon working electrode, Ag|AgCl|3M NaCl reference electrode and Pt counter electrode. For the measurement of EE2 by SWV a calibration equation was ΔI (µA) = 1.436 + 2.382 CEE2 with a correlation coefficient of 0.998, over the concentration range of 0.3?50 µM with a limit of detection of 0.11µM. Our analysis demonstrated that EE2 disappeared almost completely in the reaction mixture after 10 min treatment. These results strongly suggest that HRP is effective in removing the estrogenic activities of EE2 in water samples.
Fil: Scala Benuzzi, María Luz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Martinez, Noelia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Pereira, Sirley Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
3 rd International Meeting on Pharmaceutical Sciences
Córdoba
Argentina
Society of Pharmaceutical Sciences and Research
description Ethinylestradiol (EE2) is a synthetic estrogen used in contraceptive therapy. The presence of this compound in the environment has been attributed to their incomplete removal in wastewater treatment plant processes. The purpose of this research was to optimize the Horseradish peroxidase (HRP) enzyme-catalyzed process for removal of EE2 from synthetic water (purified deionized water) and the monitoring of the enzymatic process by square-wave voltammetry (SWV). In this study, HRP catalyzes the oxidation of EE2 in presence of H2O2 and the products formed are polymerized through a non-enzymatic process which leads to the formation of high molecular weight polymers. These low-solubility polymers can be removed from wastewater by co-precipitation, sorption to solids, sedimentation or filtration. The reaction mixture was prepared by diluting stock solution of a EE2 to a concentration of 0.5 µM, in 10 mL of 0.01 M citrate buffer pH 5.0 containing HRP 0.02 U mL-1. The reaction was initiated by adding 1 mM of H2O2. Then, after stirring for 10 min, the sample was centrifugated and EE2 was quantified by SWV. The enzymatic process was monitored by SWV using a three electrodes system: glassy carbon working electrode, Ag|AgCl|3M NaCl reference electrode and Pt counter electrode. For the measurement of EE2 by SWV a calibration equation was ΔI (µA) = 1.436 + 2.382 CEE2 with a correlation coefficient of 0.998, over the concentration range of 0.3?50 µM with a limit of detection of 0.11µM. Our analysis demonstrated that EE2 disappeared almost completely in the reaction mixture after 10 min treatment. These results strongly suggest that HRP is effective in removing the estrogenic activities of EE2 in water samples.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/290206
Electrochemical monitoring of ethinylestradiol oxidation by the horseradish peroxidase enzyme in water; 3 rd International Meeting on Pharmaceutical Sciences; Córdoba; Argentina; 2014; 1-8
0975-8232
CONICET Digital
CONICET
url http://hdl.handle.net/11336/290206
identifier_str_mv Electrochemical monitoring of ethinylestradiol oxidation by the horseradish peroxidase enzyme in water; 3 rd International Meeting on Pharmaceutical Sciences; Córdoba; Argentina; 2014; 1-8
0975-8232
CONICET Digital
CONICET
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language eng
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Society of Pharmaceutical Sciences and Research
publisher.none.fl_str_mv Society of Pharmaceutical Sciences and Research
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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