BIL7 enhances plant growth by regulating the transcription factor BIL1/BZR1 during brassinosteroid signaling ‡

Autores
Miyaji, Tomoko; Yamagami, Ayumi; Nakamura, Yusuke; Nishida, Kaisei; Tachibana, Ryo; Surina, Surina; Fujioka, Shozo; Garcia Hourquet, Mariano; Mora Garcia, Santiago; Nosaki, Shohei; Miyakawa, Takuya; Tanokura, Masaru; Matsui, Minami; Osada, Hiroyuki; Shinozaki, Kazuo; Asami, Tadao; Nakano, Takeshi
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Brassinosteroids (BRs) are plant steroid hormones that regulate plant development and environmental responses. BIL1/BZR1, a master transcription factor that regulates approximately 3000 genes in the BR signaling pathway, is transported to the nucleus from the cytosol in response to BR signaling; however, the molecular mechanism underlying this process is unknown. Here, we identify a novel BR signaling factor, BIL7, that enhances plant growth and positively regulates the nuclear accumulation of BIL1/BZR1 in Arabidopsis thaliana. BIL7-overexpressing plants were resistant to the BR biosynthesis inhibitor Brz and taller than wild-type (WT) plants were due to increased cell division. BIL7 is mainly localized to the plasma membrane, but during the early stages of cell growth, it was also localized to the nucleus. BIL7 was directly phosphorylated by the kinase BIN2, and nuclear localization of BIL7 was enhanced by the BIN2 inhibitor bikinin. BIL7 was found to bind to BIL1/BZR1, and nuclear accumulation of BIL1/BZR1 was strongly enhanced by BIL7 overexpression. Finally, double overexpression of BIL1/BZR1 and BIL7 led to greatly elongated hypocotyls in the presence of Brz. These findings suggest that BIL7 mediates nuclear accumulation of BIL1/BZR1, which activates inflorescence elongation in plants via BR signaling.
Fil: Miyaji, Tomoko. Riken Center For Sustainable Resource Science; Japón
Fil: Yamagami, Ayumi. Riken Center For Sustainable Resource Science; Japón
Fil: Nakamura, Yusuke. Kyoto University; Japón
Fil: Nishida, Kaisei. Kyoto University; Japón
Fil: Tachibana, Ryo. Kyoto University; Japón
Fil: Surina, Surina. Kyoto University; Japón
Fil: Fujioka, Shozo. Riken Center For Sustainable Resource Science; Japón
Fil: Garcia Hourquet, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Mora Garcia, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Nosaki, Shohei. Kyoto University; Japón
Fil: Miyakawa, Takuya. Kyoto University; Japón
Fil: Tanokura, Masaru. Kyoto University; Japón
Fil: Matsui, Minami. Riken Center For Sustainable Resource Science; Japón
Fil: Osada, Hiroyuki. Riken Center For Sustainable Resource Science; Japón
Fil: Shinozaki, Kazuo. Riken Center For Sustainable Resource Science; Japón
Fil: Asami, Tadao. Kyoto University; Japón
Fil: Nakano, Takeshi. Kyoto University; Japón
Materia
BRASSINOSTEROIDS
BIL7
BIN2
BZR1
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/290631
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/290631
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling BIL7 enhances plant growth by regulating the transcription factor BIL1/BZR1 during brassinosteroid signaling ‡Miyaji, TomokoYamagami, AyumiNakamura, YusukeNishida, KaiseiTachibana, RyoSurina, SurinaFujioka, ShozoGarcia Hourquet, MarianoMora Garcia, SantiagoNosaki, ShoheiMiyakawa, TakuyaTanokura, MasaruMatsui, MinamiOsada, HiroyukiShinozaki, KazuoAsami, TadaoNakano, TakeshiBRASSINOSTEROIDSBIL7BIN2BZR1https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Brassinosteroids (BRs) are plant steroid hormones that regulate plant development and environmental responses. BIL1/BZR1, a master transcription factor that regulates approximately 3000 genes in the BR signaling pathway, is transported to the nucleus from the cytosol in response to BR signaling; however, the molecular mechanism underlying this process is unknown. Here, we identify a novel BR signaling factor, BIL7, that enhances plant growth and positively regulates the nuclear accumulation of BIL1/BZR1 in Arabidopsis thaliana. BIL7-overexpressing plants were resistant to the BR biosynthesis inhibitor Brz and taller than wild-type (WT) plants were due to increased cell division. BIL7 is mainly localized to the plasma membrane, but during the early stages of cell growth, it was also localized to the nucleus. BIL7 was directly phosphorylated by the kinase BIN2, and nuclear localization of BIL7 was enhanced by the BIN2 inhibitor bikinin. BIL7 was found to bind to BIL1/BZR1, and nuclear accumulation of BIL1/BZR1 was strongly enhanced by BIL7 overexpression. Finally, double overexpression of BIL1/BZR1 and BIL7 led to greatly elongated hypocotyls in the presence of Brz. These findings suggest that BIL7 mediates nuclear accumulation of BIL1/BZR1, which activates inflorescence elongation in plants via BR signaling.Fil: Miyaji, Tomoko. Riken Center For Sustainable Resource Science; JapónFil: Yamagami, Ayumi. Riken Center For Sustainable Resource Science; JapónFil: Nakamura, Yusuke. Kyoto University; JapónFil: Nishida, Kaisei. Kyoto University; JapónFil: Tachibana, Ryo. Kyoto University; JapónFil: Surina, Surina. Kyoto University; JapónFil: Fujioka, Shozo. Riken Center For Sustainable Resource Science; JapónFil: Garcia Hourquet, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Mora Garcia, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Nosaki, Shohei. Kyoto University; JapónFil: Miyakawa, Takuya. Kyoto University; JapónFil: Tanokura, Masaru. Kyoto University; JapónFil: Matsui, Minami. Riken Center For Sustainable Resource Science; JapónFil: Osada, Hiroyuki. Riken Center For Sustainable Resource Science; JapónFil: Shinozaki, Kazuo. Riken Center For Sustainable Resource Science; JapónFil: Asami, Tadao. Kyoto University; JapónFil: Nakano, Takeshi. Kyoto University; JapónWiley Blackwell Publishing, Inc2024-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/290631Miyaji, Tomoko; Yamagami, Ayumi; Nakamura, Yusuke; Nishida, Kaisei; Tachibana, Ryo; et al.; BIL7 enhances plant growth by regulating the transcription factor BIL1/BZR1 during brassinosteroid signaling ‡; Wiley Blackwell Publishing, Inc; Plant Journal; 121; 2; 12-2024; 1-180960-7412CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/tpj.17212info:eu-repo/semantics/altIdentifier/doi/10.1111/tpj.17212info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:32:32Zoai:ri.conicet.gov.ar:11336/290631instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:32:32.295CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv BIL7 enhances plant growth by regulating the transcription factor BIL1/BZR1 during brassinosteroid signaling ‡
title BIL7 enhances plant growth by regulating the transcription factor BIL1/BZR1 during brassinosteroid signaling ‡
spellingShingle BIL7 enhances plant growth by regulating the transcription factor BIL1/BZR1 during brassinosteroid signaling ‡
Miyaji, Tomoko
BRASSINOSTEROIDS
BIL7
BIN2
BZR1
title_short BIL7 enhances plant growth by regulating the transcription factor BIL1/BZR1 during brassinosteroid signaling ‡
title_full BIL7 enhances plant growth by regulating the transcription factor BIL1/BZR1 during brassinosteroid signaling ‡
title_fullStr BIL7 enhances plant growth by regulating the transcription factor BIL1/BZR1 during brassinosteroid signaling ‡
title_full_unstemmed BIL7 enhances plant growth by regulating the transcription factor BIL1/BZR1 during brassinosteroid signaling ‡
title_sort BIL7 enhances plant growth by regulating the transcription factor BIL1/BZR1 during brassinosteroid signaling ‡
dc.creator.none.fl_str_mv Miyaji, Tomoko
Yamagami, Ayumi
Nakamura, Yusuke
Nishida, Kaisei
Tachibana, Ryo
Surina, Surina
Fujioka, Shozo
Garcia Hourquet, Mariano
Mora Garcia, Santiago
Nosaki, Shohei
Miyakawa, Takuya
Tanokura, Masaru
Matsui, Minami
Osada, Hiroyuki
Shinozaki, Kazuo
Asami, Tadao
Nakano, Takeshi
author Miyaji, Tomoko
author_facet Miyaji, Tomoko
Yamagami, Ayumi
Nakamura, Yusuke
Nishida, Kaisei
Tachibana, Ryo
Surina, Surina
Fujioka, Shozo
Garcia Hourquet, Mariano
Mora Garcia, Santiago
Nosaki, Shohei
Miyakawa, Takuya
Tanokura, Masaru
Matsui, Minami
Osada, Hiroyuki
Shinozaki, Kazuo
Asami, Tadao
Nakano, Takeshi
author_role author
author2 Yamagami, Ayumi
Nakamura, Yusuke
Nishida, Kaisei
Tachibana, Ryo
Surina, Surina
Fujioka, Shozo
Garcia Hourquet, Mariano
Mora Garcia, Santiago
Nosaki, Shohei
Miyakawa, Takuya
Tanokura, Masaru
Matsui, Minami
Osada, Hiroyuki
Shinozaki, Kazuo
Asami, Tadao
Nakano, Takeshi
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BRASSINOSTEROIDS
BIL7
BIN2
BZR1
topic BRASSINOSTEROIDS
BIL7
BIN2
BZR1
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Brassinosteroids (BRs) are plant steroid hormones that regulate plant development and environmental responses. BIL1/BZR1, a master transcription factor that regulates approximately 3000 genes in the BR signaling pathway, is transported to the nucleus from the cytosol in response to BR signaling; however, the molecular mechanism underlying this process is unknown. Here, we identify a novel BR signaling factor, BIL7, that enhances plant growth and positively regulates the nuclear accumulation of BIL1/BZR1 in Arabidopsis thaliana. BIL7-overexpressing plants were resistant to the BR biosynthesis inhibitor Brz and taller than wild-type (WT) plants were due to increased cell division. BIL7 is mainly localized to the plasma membrane, but during the early stages of cell growth, it was also localized to the nucleus. BIL7 was directly phosphorylated by the kinase BIN2, and nuclear localization of BIL7 was enhanced by the BIN2 inhibitor bikinin. BIL7 was found to bind to BIL1/BZR1, and nuclear accumulation of BIL1/BZR1 was strongly enhanced by BIL7 overexpression. Finally, double overexpression of BIL1/BZR1 and BIL7 led to greatly elongated hypocotyls in the presence of Brz. These findings suggest that BIL7 mediates nuclear accumulation of BIL1/BZR1, which activates inflorescence elongation in plants via BR signaling.
Fil: Miyaji, Tomoko. Riken Center For Sustainable Resource Science; Japón
Fil: Yamagami, Ayumi. Riken Center For Sustainable Resource Science; Japón
Fil: Nakamura, Yusuke. Kyoto University; Japón
Fil: Nishida, Kaisei. Kyoto University; Japón
Fil: Tachibana, Ryo. Kyoto University; Japón
Fil: Surina, Surina. Kyoto University; Japón
Fil: Fujioka, Shozo. Riken Center For Sustainable Resource Science; Japón
Fil: Garcia Hourquet, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Mora Garcia, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Nosaki, Shohei. Kyoto University; Japón
Fil: Miyakawa, Takuya. Kyoto University; Japón
Fil: Tanokura, Masaru. Kyoto University; Japón
Fil: Matsui, Minami. Riken Center For Sustainable Resource Science; Japón
Fil: Osada, Hiroyuki. Riken Center For Sustainable Resource Science; Japón
Fil: Shinozaki, Kazuo. Riken Center For Sustainable Resource Science; Japón
Fil: Asami, Tadao. Kyoto University; Japón
Fil: Nakano, Takeshi. Kyoto University; Japón
description Brassinosteroids (BRs) are plant steroid hormones that regulate plant development and environmental responses. BIL1/BZR1, a master transcription factor that regulates approximately 3000 genes in the BR signaling pathway, is transported to the nucleus from the cytosol in response to BR signaling; however, the molecular mechanism underlying this process is unknown. Here, we identify a novel BR signaling factor, BIL7, that enhances plant growth and positively regulates the nuclear accumulation of BIL1/BZR1 in Arabidopsis thaliana. BIL7-overexpressing plants were resistant to the BR biosynthesis inhibitor Brz and taller than wild-type (WT) plants were due to increased cell division. BIL7 is mainly localized to the plasma membrane, but during the early stages of cell growth, it was also localized to the nucleus. BIL7 was directly phosphorylated by the kinase BIN2, and nuclear localization of BIL7 was enhanced by the BIN2 inhibitor bikinin. BIL7 was found to bind to BIL1/BZR1, and nuclear accumulation of BIL1/BZR1 was strongly enhanced by BIL7 overexpression. Finally, double overexpression of BIL1/BZR1 and BIL7 led to greatly elongated hypocotyls in the presence of Brz. These findings suggest that BIL7 mediates nuclear accumulation of BIL1/BZR1, which activates inflorescence elongation in plants via BR signaling.
publishDate 2024
dc.date.none.fl_str_mv 2024-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/290631
Miyaji, Tomoko; Yamagami, Ayumi; Nakamura, Yusuke; Nishida, Kaisei; Tachibana, Ryo; et al.; BIL7 enhances plant growth by regulating the transcription factor BIL1/BZR1 during brassinosteroid signaling ‡; Wiley Blackwell Publishing, Inc; Plant Journal; 121; 2; 12-2024; 1-18
0960-7412
CONICET Digital
CONICET
url http://hdl.handle.net/11336/290631
identifier_str_mv Miyaji, Tomoko; Yamagami, Ayumi; Nakamura, Yusuke; Nishida, Kaisei; Tachibana, Ryo; et al.; BIL7 enhances plant growth by regulating the transcription factor BIL1/BZR1 during brassinosteroid signaling ‡; Wiley Blackwell Publishing, Inc; Plant Journal; 121; 2; 12-2024; 1-18
0960-7412
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/tpj.17212
info:eu-repo/semantics/altIdentifier/doi/10.1111/tpj.17212
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24418

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