Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins

Autores
Kotsovolos, Nikolaos; Schnittger, Leonhard; Sibeko Matjila, Kgomotso
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
As an obligate intracellular parasite, Theileria parva is strictly dependent on its host for nutrient acquisition. Transport proteins are expected to play a crucial role in the influx of essential nutrients to sustain the parasite's rapid growth. Unfortunately, the T. parva transportome is still not comprehensively elucidated, and plagued by the presence of uncharacterized proteins. In this study, we employed a combination of approaches including sequence orthology and structural similarity to identify 188 proteins predicted to be involved in transport-related processes. Among these, 24 were uncharacterized proteins, and 17 of them could be assigned a tentative annotation. Furthermore, the localization of these 188 proteins was investigated, resulting in their assignment to seven cellular compartments. Screening of the proteomes of other Theileria species, T. annulata, T. orientalis, and T. equi revealed that all 188 proteins were present in both transforming and non-transforming Theileria parasites. Among the 188 potential transport-related proteins, 45 were associated with transmembrane transport and most of them (87 %) are conserved across phylum Apicomplexa.
Fil: Kotsovolos, Nikolaos. University of Pretoria; Sudáfrica
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Patobiología Veterinaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Patobiología Veterinaria; Argentina
Fil: Sibeko Matjila, Kgomotso. University of Pretoria; Sudáfrica
Materia
BIOINFORMATICS
PROTEIN ANNOTATION
PROTEIN SEQUENCE HOMOLOGY
PROTEIN STRUCTURE HOMOLOGY
THEILERIA PARVA
TRANSPORT PROTEIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/287360
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteinsKotsovolos, NikolaosSchnittger, LeonhardSibeko Matjila, KgomotsoBIOINFORMATICSPROTEIN ANNOTATIONPROTEIN SEQUENCE HOMOLOGYPROTEIN STRUCTURE HOMOLOGYTHEILERIA PARVATRANSPORT PROTEINhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4As an obligate intracellular parasite, Theileria parva is strictly dependent on its host for nutrient acquisition. Transport proteins are expected to play a crucial role in the influx of essential nutrients to sustain the parasite's rapid growth. Unfortunately, the T. parva transportome is still not comprehensively elucidated, and plagued by the presence of uncharacterized proteins. In this study, we employed a combination of approaches including sequence orthology and structural similarity to identify 188 proteins predicted to be involved in transport-related processes. Among these, 24 were uncharacterized proteins, and 17 of them could be assigned a tentative annotation. Furthermore, the localization of these 188 proteins was investigated, resulting in their assignment to seven cellular compartments. Screening of the proteomes of other Theileria species, T. annulata, T. orientalis, and T. equi revealed that all 188 proteins were present in both transforming and non-transforming Theileria parasites. Among the 188 potential transport-related proteins, 45 were associated with transmembrane transport and most of them (87 %) are conserved across phylum Apicomplexa.Fil: Kotsovolos, Nikolaos. University of Pretoria; SudáfricaFil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Patobiología Veterinaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Patobiología Veterinaria; ArgentinaFil: Sibeko Matjila, Kgomotso. University of Pretoria; SudáfricaElsevier2026-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/287360Kotsovolos, Nikolaos; Schnittger, Leonhard; Sibeko Matjila, Kgomotso; Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins; Elsevier; Computational Biology And Chemistry; 120; 2-2026; 1-141476-9271CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S1476927125003147info:eu-repo/semantics/altIdentifier/doi/10.1016/j.compbiolchem.2025.108653info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:32:36Zoai:ri.conicet.gov.ar:11336/287360instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:32:36.269CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins
title Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins
spellingShingle Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins
Kotsovolos, Nikolaos
BIOINFORMATICS
PROTEIN ANNOTATION
PROTEIN SEQUENCE HOMOLOGY
PROTEIN STRUCTURE HOMOLOGY
THEILERIA PARVA
TRANSPORT PROTEIN
title_short Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins
title_full Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins
title_fullStr Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins
title_full_unstemmed Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins
title_sort Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins
dc.creator.none.fl_str_mv Kotsovolos, Nikolaos
Schnittger, Leonhard
Sibeko Matjila, Kgomotso
author Kotsovolos, Nikolaos
author_facet Kotsovolos, Nikolaos
Schnittger, Leonhard
Sibeko Matjila, Kgomotso
author_role author
author2 Schnittger, Leonhard
Sibeko Matjila, Kgomotso
author2_role author
author
dc.subject.none.fl_str_mv BIOINFORMATICS
PROTEIN ANNOTATION
PROTEIN SEQUENCE HOMOLOGY
PROTEIN STRUCTURE HOMOLOGY
THEILERIA PARVA
TRANSPORT PROTEIN
topic BIOINFORMATICS
PROTEIN ANNOTATION
PROTEIN SEQUENCE HOMOLOGY
PROTEIN STRUCTURE HOMOLOGY
THEILERIA PARVA
TRANSPORT PROTEIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv As an obligate intracellular parasite, Theileria parva is strictly dependent on its host for nutrient acquisition. Transport proteins are expected to play a crucial role in the influx of essential nutrients to sustain the parasite's rapid growth. Unfortunately, the T. parva transportome is still not comprehensively elucidated, and plagued by the presence of uncharacterized proteins. In this study, we employed a combination of approaches including sequence orthology and structural similarity to identify 188 proteins predicted to be involved in transport-related processes. Among these, 24 were uncharacterized proteins, and 17 of them could be assigned a tentative annotation. Furthermore, the localization of these 188 proteins was investigated, resulting in their assignment to seven cellular compartments. Screening of the proteomes of other Theileria species, T. annulata, T. orientalis, and T. equi revealed that all 188 proteins were present in both transforming and non-transforming Theileria parasites. Among the 188 potential transport-related proteins, 45 were associated with transmembrane transport and most of them (87 %) are conserved across phylum Apicomplexa.
Fil: Kotsovolos, Nikolaos. University of Pretoria; Sudáfrica
Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Patobiología Veterinaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Patobiología Veterinaria; Argentina
Fil: Sibeko Matjila, Kgomotso. University of Pretoria; Sudáfrica
description As an obligate intracellular parasite, Theileria parva is strictly dependent on its host for nutrient acquisition. Transport proteins are expected to play a crucial role in the influx of essential nutrients to sustain the parasite's rapid growth. Unfortunately, the T. parva transportome is still not comprehensively elucidated, and plagued by the presence of uncharacterized proteins. In this study, we employed a combination of approaches including sequence orthology and structural similarity to identify 188 proteins predicted to be involved in transport-related processes. Among these, 24 were uncharacterized proteins, and 17 of them could be assigned a tentative annotation. Furthermore, the localization of these 188 proteins was investigated, resulting in their assignment to seven cellular compartments. Screening of the proteomes of other Theileria species, T. annulata, T. orientalis, and T. equi revealed that all 188 proteins were present in both transforming and non-transforming Theileria parasites. Among the 188 potential transport-related proteins, 45 were associated with transmembrane transport and most of them (87 %) are conserved across phylum Apicomplexa.
publishDate 2026
dc.date.none.fl_str_mv 2026-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/287360
Kotsovolos, Nikolaos; Schnittger, Leonhard; Sibeko Matjila, Kgomotso; Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins; Elsevier; Computational Biology And Chemistry; 120; 2-2026; 1-14
1476-9271
CONICET Digital
CONICET
url http://hdl.handle.net/11336/287360
identifier_str_mv Kotsovolos, Nikolaos; Schnittger, Leonhard; Sibeko Matjila, Kgomotso; Sequence- and structure-based bioinformatic screening for potential Theileria parva transport-related proteins; Elsevier; Computational Biology And Chemistry; 120; 2-2026; 1-14
1476-9271
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S1476927125003147
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.compbiolchem.2025.108653
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1874774122148397056
score 13.265058

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