Does nitrogen source influence cadmium distribution in Arabidopsis plants?

Autores
Vazquez, Analia; Recalde, Laura; Cabrera, Andrea; Groppa, María Daniela; Benavides, Maria Patricia
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The purpose of the present work was to study the effect of the nitrogen source (NO3 - vs NH4 +) on cadmium (Cd) uptake, translocation and partition and its associated toxicity in hydroponically-grown Arabidopsis plants. After a short growth period on a complete Hoagland nutrient solution, Arabidopsis seedlings continued in the same growth medium (NA) or were switched to NO3 – (N) or NH4 + (A) as sole N sources and supplied with 2.5 μM Cd. Unrelated to the nitrogen source, Cd reached higher levels in roots than in leaves. However, when ammonium was the source of nitrogen, Cd accumulation in roots was lower than in N or NA medium and the metal translocation to the aerial part was restricted, reaching values 25%–35% below the levels observed in plants grown with N or NA. Cadmium negatively affected chlorophyll content and PSII quantum yield, independently of the nitrogen source, with the highest decrease (35%) under NA treatment. Proline content increased, either with NA, N or A supplied in the presence of Cd, whereas a rise in total anthocyanin content was clearly favored when ammonium was the source of nitrogen, with or without Cd. In leaves, while NIA1 and NIA2 expression was markedly reduced by Cd in the presence of N or NA, ammonium source slightly reduced NIA1 expression but greatly upregulated NIA2 expression upon Cd exposure. The decay in NR activity was independent of the nitrogen source when Cd was applied and this decay was accompanied by a great increase in NH4 + levels either with nitrates or ammonium in the medium in the presence of Cd. Only NIA1 was detected in roots and its expression, together with NR activity and nitrates levels, was the highest in N medium devoid of Cd. The possibility of reducing Cd health risks through nitrogen fertilization practices is discussed.
Fil: Vazquez, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Recalde, Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Cabrera, Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Groppa, María Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Benavides, Maria Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Materia
ARABIDOPSIS
CADMIUM
NIA GENES
NITRATE REDUCTASE
NITROGEN
UPTAKE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/144253
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/144253
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Does nitrogen source influence cadmium distribution in Arabidopsis plants?Vazquez, AnaliaRecalde, LauraCabrera, AndreaGroppa, María DanielaBenavides, Maria PatriciaARABIDOPSISCADMIUMNIA GENESNITRATE REDUCTASENITROGENUPTAKEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The purpose of the present work was to study the effect of the nitrogen source (NO3 - vs NH4 +) on cadmium (Cd) uptake, translocation and partition and its associated toxicity in hydroponically-grown Arabidopsis plants. After a short growth period on a complete Hoagland nutrient solution, Arabidopsis seedlings continued in the same growth medium (NA) or were switched to NO3 – (N) or NH4 + (A) as sole N sources and supplied with 2.5 μM Cd. Unrelated to the nitrogen source, Cd reached higher levels in roots than in leaves. However, when ammonium was the source of nitrogen, Cd accumulation in roots was lower than in N or NA medium and the metal translocation to the aerial part was restricted, reaching values 25%–35% below the levels observed in plants grown with N or NA. Cadmium negatively affected chlorophyll content and PSII quantum yield, independently of the nitrogen source, with the highest decrease (35%) under NA treatment. Proline content increased, either with NA, N or A supplied in the presence of Cd, whereas a rise in total anthocyanin content was clearly favored when ammonium was the source of nitrogen, with or without Cd. In leaves, while NIA1 and NIA2 expression was markedly reduced by Cd in the presence of N or NA, ammonium source slightly reduced NIA1 expression but greatly upregulated NIA2 expression upon Cd exposure. The decay in NR activity was independent of the nitrogen source when Cd was applied and this decay was accompanied by a great increase in NH4 + levels either with nitrates or ammonium in the medium in the presence of Cd. Only NIA1 was detected in roots and its expression, together with NR activity and nitrates levels, was the highest in N medium devoid of Cd. The possibility of reducing Cd health risks through nitrogen fertilization practices is discussed.Fil: Vazquez, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Recalde, Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; ArgentinaFil: Cabrera, Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; ArgentinaFil: Groppa, María Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaFil: Benavides, Maria Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; ArgentinaAcademic Press Inc Elsevier Science2020-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/144253Vazquez, Analia; Recalde, Laura; Cabrera, Andrea; Groppa, María Daniela; Benavides, Maria Patricia; Does nitrogen source influence cadmium distribution in Arabidopsis plants?; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 191; 3-2020; 1-90147-6513CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0147651320300026info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2020.110163info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T15:32:30Zoai:ri.conicet.gov.ar:11336/144253instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 15:32:30.36CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Does nitrogen source influence cadmium distribution in Arabidopsis plants?
title Does nitrogen source influence cadmium distribution in Arabidopsis plants?
spellingShingle Does nitrogen source influence cadmium distribution in Arabidopsis plants?
Vazquez, Analia
ARABIDOPSIS
CADMIUM
NIA GENES
NITRATE REDUCTASE
NITROGEN
UPTAKE
title_short Does nitrogen source influence cadmium distribution in Arabidopsis plants?
title_full Does nitrogen source influence cadmium distribution in Arabidopsis plants?
title_fullStr Does nitrogen source influence cadmium distribution in Arabidopsis plants?
title_full_unstemmed Does nitrogen source influence cadmium distribution in Arabidopsis plants?
title_sort Does nitrogen source influence cadmium distribution in Arabidopsis plants?
dc.creator.none.fl_str_mv Vazquez, Analia
Recalde, Laura
Cabrera, Andrea
Groppa, María Daniela
Benavides, Maria Patricia
author Vazquez, Analia
author_facet Vazquez, Analia
Recalde, Laura
Cabrera, Andrea
Groppa, María Daniela
Benavides, Maria Patricia
author_role author
author2 Recalde, Laura
Cabrera, Andrea
Groppa, María Daniela
Benavides, Maria Patricia
author2_role author
author
author
author
dc.subject.none.fl_str_mv ARABIDOPSIS
CADMIUM
NIA GENES
NITRATE REDUCTASE
NITROGEN
UPTAKE
topic ARABIDOPSIS
CADMIUM
NIA GENES
NITRATE REDUCTASE
NITROGEN
UPTAKE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The purpose of the present work was to study the effect of the nitrogen source (NO3 - vs NH4 +) on cadmium (Cd) uptake, translocation and partition and its associated toxicity in hydroponically-grown Arabidopsis plants. After a short growth period on a complete Hoagland nutrient solution, Arabidopsis seedlings continued in the same growth medium (NA) or were switched to NO3 – (N) or NH4 + (A) as sole N sources and supplied with 2.5 μM Cd. Unrelated to the nitrogen source, Cd reached higher levels in roots than in leaves. However, when ammonium was the source of nitrogen, Cd accumulation in roots was lower than in N or NA medium and the metal translocation to the aerial part was restricted, reaching values 25%–35% below the levels observed in plants grown with N or NA. Cadmium negatively affected chlorophyll content and PSII quantum yield, independently of the nitrogen source, with the highest decrease (35%) under NA treatment. Proline content increased, either with NA, N or A supplied in the presence of Cd, whereas a rise in total anthocyanin content was clearly favored when ammonium was the source of nitrogen, with or without Cd. In leaves, while NIA1 and NIA2 expression was markedly reduced by Cd in the presence of N or NA, ammonium source slightly reduced NIA1 expression but greatly upregulated NIA2 expression upon Cd exposure. The decay in NR activity was independent of the nitrogen source when Cd was applied and this decay was accompanied by a great increase in NH4 + levels either with nitrates or ammonium in the medium in the presence of Cd. Only NIA1 was detected in roots and its expression, together with NR activity and nitrates levels, was the highest in N medium devoid of Cd. The possibility of reducing Cd health risks through nitrogen fertilization practices is discussed.
Fil: Vazquez, Analia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Recalde, Laura. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Cabrera, Andrea. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina
Fil: Groppa, María Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
Fil: Benavides, Maria Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina
description The purpose of the present work was to study the effect of the nitrogen source (NO3 - vs NH4 +) on cadmium (Cd) uptake, translocation and partition and its associated toxicity in hydroponically-grown Arabidopsis plants. After a short growth period on a complete Hoagland nutrient solution, Arabidopsis seedlings continued in the same growth medium (NA) or were switched to NO3 – (N) or NH4 + (A) as sole N sources and supplied with 2.5 μM Cd. Unrelated to the nitrogen source, Cd reached higher levels in roots than in leaves. However, when ammonium was the source of nitrogen, Cd accumulation in roots was lower than in N or NA medium and the metal translocation to the aerial part was restricted, reaching values 25%–35% below the levels observed in plants grown with N or NA. Cadmium negatively affected chlorophyll content and PSII quantum yield, independently of the nitrogen source, with the highest decrease (35%) under NA treatment. Proline content increased, either with NA, N or A supplied in the presence of Cd, whereas a rise in total anthocyanin content was clearly favored when ammonium was the source of nitrogen, with or without Cd. In leaves, while NIA1 and NIA2 expression was markedly reduced by Cd in the presence of N or NA, ammonium source slightly reduced NIA1 expression but greatly upregulated NIA2 expression upon Cd exposure. The decay in NR activity was independent of the nitrogen source when Cd was applied and this decay was accompanied by a great increase in NH4 + levels either with nitrates or ammonium in the medium in the presence of Cd. Only NIA1 was detected in roots and its expression, together with NR activity and nitrates levels, was the highest in N medium devoid of Cd. The possibility of reducing Cd health risks through nitrogen fertilization practices is discussed.
publishDate 2020
dc.date.none.fl_str_mv 2020-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/144253
Vazquez, Analia; Recalde, Laura; Cabrera, Andrea; Groppa, María Daniela; Benavides, Maria Patricia; Does nitrogen source influence cadmium distribution in Arabidopsis plants?; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 191; 3-2020; 1-9
0147-6513
CONICET Digital
CONICET
url http://hdl.handle.net/11336/144253
identifier_str_mv Vazquez, Analia; Recalde, Laura; Cabrera, Andrea; Groppa, María Daniela; Benavides, Maria Patricia; Does nitrogen source influence cadmium distribution in Arabidopsis plants?; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 191; 3-2020; 1-9
0147-6513
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0147651320300026
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecoenv.2020.110163
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.364332

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