Characterization of EPS from Ligilactobacillus salivarius CRL 2217 by transmission and scanning electron microscopy techniques

Autores
Emmert, German Ezequiel; Argañaraz Martínez, Fernando Eloy; Grande, Sonia María Mercedes; Babot, Jaime Daniel; Perez Chaia, Adriana Beatriz
Año de publicación
2024
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Bacterial exopolysaccharides (EPS) are macromolecules usually synthesized during growth under certain incubation conditions. Depending on their location, they may be of a capsular type (tightly bound to the cell surface) and/or secreted to the extracellular environment (with weak bonds or completely secreted). These compounds can exhibit diverse functional and technological properties based on their structure; e.g.: they may possess antioxidant, prebiotic, anti-inflammatory, immunostimulant, or antibacterial properties. The objective of this study was to characterize the EPS produced by Ligilactobacillus salivarius (Lg. salivarius) CRL 2217 in a modified MRS broth employing scanning electron microscopy (SEM) and transmission electron microscopy (TEM) techniques. Growth curves of Lg. salivarius CRL 2217 were performed in MRS broth (control) and MRS modified by replacing glucose with 5% sucrose as the energy source. Samples were taken at different times to determine O.D.560nm, CFU/mL, EPS concentration was determined using the DuBois et al. (1956) method with D-glucose as a standard, and residual glucose using the glucose oxidase method (Wiener Lab., Rosario, Argentina). The results obtained indicated that EPS synthesis was stimulated in the medium with sucrose. It occurred simultaneously with cell growth and reached a maximum peak concentration of 1.40 ± 0.04 mg/mL at 16 h. In MRS with glucose, a basal EPS production of approximately 0.2 mg/mL was determined along the curve. A higher electron density was observed around the bacteria in the medium with sucrose when the cells were evaluated in the late exponential phase in both media using TEM. In addition, morphological changes on the cells were observed in the different culture media. The extracted EPS was purified, lyophilized, and analyzed by SEM at different magnifications. A structure of meshes and superimposed layers, which could confer a good water retention capacity, was observed. Thus, the water retention capacity and the solubility in water of the EPS were evaluated according to the method of Du et al. (2018), obtaining values of 445.35 ± 12.23 and 98.24 ± 0.61 %, respectively. The results of this study suggest that the EPS of Lg. salivarius CRL 2217, synthesized in an MRS medium with sucrose as the energy source, was secreted around the bacterium in a discontinuous manner and may be weakly bound to the cell surface. It exhibits a lattice structure that confers excellent solubility and water-holding capacity. This EPS could be a potential additive for use as a thickener, stabilizer, and emulsifier in farm animal feed products. Also, its potential in drug delivery could be explored.
Fil: Emmert, German Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: Argañaraz Martínez, Fernando Eloy. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: Grande, Sonia María Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: Babot, Jaime Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Perez Chaia, Adriana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
VIII Congreso Argentino de Microscopía
Tafí Viejo
Argentina
Sociedad Argentina de Microscopía
Materia
TEM
SEM
EXOPOLYSACCHARIDE
LACTIC ACID BACTERIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/287767
Acceder
id CONICETDig_8be335ec026e68422e55e65485f47c9f
oai_identifier_str oai:ri.conicet.gov.ar:11336/287767
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of EPS from Ligilactobacillus salivarius CRL 2217 by transmission and scanning electron microscopy techniquesEmmert, German EzequielArgañaraz Martínez, Fernando EloyGrande, Sonia María MercedesBabot, Jaime DanielPerez Chaia, Adriana BeatrizTEMSEMEXOPOLYSACCHARIDELACTIC ACID BACTERIAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Bacterial exopolysaccharides (EPS) are macromolecules usually synthesized during growth under certain incubation conditions. Depending on their location, they may be of a capsular type (tightly bound to the cell surface) and/or secreted to the extracellular environment (with weak bonds or completely secreted). These compounds can exhibit diverse functional and technological properties based on their structure; e.g.: they may possess antioxidant, prebiotic, anti-inflammatory, immunostimulant, or antibacterial properties. The objective of this study was to characterize the EPS produced by Ligilactobacillus salivarius (Lg. salivarius) CRL 2217 in a modified MRS broth employing scanning electron microscopy (SEM) and transmission electron microscopy (TEM) techniques. Growth curves of Lg. salivarius CRL 2217 were performed in MRS broth (control) and MRS modified by replacing glucose with 5% sucrose as the energy source. Samples were taken at different times to determine O.D.560nm, CFU/mL, EPS concentration was determined using the DuBois et al. (1956) method with D-glucose as a standard, and residual glucose using the glucose oxidase method (Wiener Lab., Rosario, Argentina). The results obtained indicated that EPS synthesis was stimulated in the medium with sucrose. It occurred simultaneously with cell growth and reached a maximum peak concentration of 1.40 ± 0.04 mg/mL at 16 h. In MRS with glucose, a basal EPS production of approximately 0.2 mg/mL was determined along the curve. A higher electron density was observed around the bacteria in the medium with sucrose when the cells were evaluated in the late exponential phase in both media using TEM. In addition, morphological changes on the cells were observed in the different culture media. The extracted EPS was purified, lyophilized, and analyzed by SEM at different magnifications. A structure of meshes and superimposed layers, which could confer a good water retention capacity, was observed. Thus, the water retention capacity and the solubility in water of the EPS were evaluated according to the method of Du et al. (2018), obtaining values of 445.35 ± 12.23 and 98.24 ± 0.61 %, respectively. The results of this study suggest that the EPS of Lg. salivarius CRL 2217, synthesized in an MRS medium with sucrose as the energy source, was secreted around the bacterium in a discontinuous manner and may be weakly bound to the cell surface. It exhibits a lattice structure that confers excellent solubility and water-holding capacity. This EPS could be a potential additive for use as a thickener, stabilizer, and emulsifier in farm animal feed products. Also, its potential in drug delivery could be explored.Fil: Emmert, German Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaFil: Argañaraz Martínez, Fernando Eloy. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaFil: Grande, Sonia María Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; ArgentinaFil: Babot, Jaime Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Perez Chaia, Adriana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaVIII Congreso Argentino de MicroscopíaTafí ViejoArgentinaSociedad Argentina de MicroscopíaSociedad Argentina de Microscopía2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/287767Characterization of EPS from Ligilactobacillus salivarius CRL 2217 by transmission and scanning electron microscopy techniques; VIII Congreso Argentino de Microscopía; Tafí Viejo; Argentina; 2024; 138-139CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.samictucuman2024.com/certificadosNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:30:26Zoai:ri.conicet.gov.ar:11336/287767instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:30:26.987CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of EPS from Ligilactobacillus salivarius CRL 2217 by transmission and scanning electron microscopy techniques
title Characterization of EPS from Ligilactobacillus salivarius CRL 2217 by transmission and scanning electron microscopy techniques
spellingShingle Characterization of EPS from Ligilactobacillus salivarius CRL 2217 by transmission and scanning electron microscopy techniques
Emmert, German Ezequiel
TEM
SEM
EXOPOLYSACCHARIDE
LACTIC ACID BACTERIA
title_short Characterization of EPS from Ligilactobacillus salivarius CRL 2217 by transmission and scanning electron microscopy techniques
title_full Characterization of EPS from Ligilactobacillus salivarius CRL 2217 by transmission and scanning electron microscopy techniques
title_fullStr Characterization of EPS from Ligilactobacillus salivarius CRL 2217 by transmission and scanning electron microscopy techniques
title_full_unstemmed Characterization of EPS from Ligilactobacillus salivarius CRL 2217 by transmission and scanning electron microscopy techniques
title_sort Characterization of EPS from Ligilactobacillus salivarius CRL 2217 by transmission and scanning electron microscopy techniques
dc.creator.none.fl_str_mv Emmert, German Ezequiel
Argañaraz Martínez, Fernando Eloy
Grande, Sonia María Mercedes
Babot, Jaime Daniel
Perez Chaia, Adriana Beatriz
author Emmert, German Ezequiel
author_facet Emmert, German Ezequiel
Argañaraz Martínez, Fernando Eloy
Grande, Sonia María Mercedes
Babot, Jaime Daniel
Perez Chaia, Adriana Beatriz
author_role author
author2 Argañaraz Martínez, Fernando Eloy
Grande, Sonia María Mercedes
Babot, Jaime Daniel
Perez Chaia, Adriana Beatriz
author2_role author
author
author
author
dc.subject.none.fl_str_mv TEM
SEM
EXOPOLYSACCHARIDE
LACTIC ACID BACTERIA
topic TEM
SEM
EXOPOLYSACCHARIDE
LACTIC ACID BACTERIA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bacterial exopolysaccharides (EPS) are macromolecules usually synthesized during growth under certain incubation conditions. Depending on their location, they may be of a capsular type (tightly bound to the cell surface) and/or secreted to the extracellular environment (with weak bonds or completely secreted). These compounds can exhibit diverse functional and technological properties based on their structure; e.g.: they may possess antioxidant, prebiotic, anti-inflammatory, immunostimulant, or antibacterial properties. The objective of this study was to characterize the EPS produced by Ligilactobacillus salivarius (Lg. salivarius) CRL 2217 in a modified MRS broth employing scanning electron microscopy (SEM) and transmission electron microscopy (TEM) techniques. Growth curves of Lg. salivarius CRL 2217 were performed in MRS broth (control) and MRS modified by replacing glucose with 5% sucrose as the energy source. Samples were taken at different times to determine O.D.560nm, CFU/mL, EPS concentration was determined using the DuBois et al. (1956) method with D-glucose as a standard, and residual glucose using the glucose oxidase method (Wiener Lab., Rosario, Argentina). The results obtained indicated that EPS synthesis was stimulated in the medium with sucrose. It occurred simultaneously with cell growth and reached a maximum peak concentration of 1.40 ± 0.04 mg/mL at 16 h. In MRS with glucose, a basal EPS production of approximately 0.2 mg/mL was determined along the curve. A higher electron density was observed around the bacteria in the medium with sucrose when the cells were evaluated in the late exponential phase in both media using TEM. In addition, morphological changes on the cells were observed in the different culture media. The extracted EPS was purified, lyophilized, and analyzed by SEM at different magnifications. A structure of meshes and superimposed layers, which could confer a good water retention capacity, was observed. Thus, the water retention capacity and the solubility in water of the EPS were evaluated according to the method of Du et al. (2018), obtaining values of 445.35 ± 12.23 and 98.24 ± 0.61 %, respectively. The results of this study suggest that the EPS of Lg. salivarius CRL 2217, synthesized in an MRS medium with sucrose as the energy source, was secreted around the bacterium in a discontinuous manner and may be weakly bound to the cell surface. It exhibits a lattice structure that confers excellent solubility and water-holding capacity. This EPS could be a potential additive for use as a thickener, stabilizer, and emulsifier in farm animal feed products. Also, its potential in drug delivery could be explored.
Fil: Emmert, German Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: Argañaraz Martínez, Fernando Eloy. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: Grande, Sonia María Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
Fil: Babot, Jaime Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Perez Chaia, Adriana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
VIII Congreso Argentino de Microscopía
Tafí Viejo
Argentina
Sociedad Argentina de Microscopía
description Bacterial exopolysaccharides (EPS) are macromolecules usually synthesized during growth under certain incubation conditions. Depending on their location, they may be of a capsular type (tightly bound to the cell surface) and/or secreted to the extracellular environment (with weak bonds or completely secreted). These compounds can exhibit diverse functional and technological properties based on their structure; e.g.: they may possess antioxidant, prebiotic, anti-inflammatory, immunostimulant, or antibacterial properties. The objective of this study was to characterize the EPS produced by Ligilactobacillus salivarius (Lg. salivarius) CRL 2217 in a modified MRS broth employing scanning electron microscopy (SEM) and transmission electron microscopy (TEM) techniques. Growth curves of Lg. salivarius CRL 2217 were performed in MRS broth (control) and MRS modified by replacing glucose with 5% sucrose as the energy source. Samples were taken at different times to determine O.D.560nm, CFU/mL, EPS concentration was determined using the DuBois et al. (1956) method with D-glucose as a standard, and residual glucose using the glucose oxidase method (Wiener Lab., Rosario, Argentina). The results obtained indicated that EPS synthesis was stimulated in the medium with sucrose. It occurred simultaneously with cell growth and reached a maximum peak concentration of 1.40 ± 0.04 mg/mL at 16 h. In MRS with glucose, a basal EPS production of approximately 0.2 mg/mL was determined along the curve. A higher electron density was observed around the bacteria in the medium with sucrose when the cells were evaluated in the late exponential phase in both media using TEM. In addition, morphological changes on the cells were observed in the different culture media. The extracted EPS was purified, lyophilized, and analyzed by SEM at different magnifications. A structure of meshes and superimposed layers, which could confer a good water retention capacity, was observed. Thus, the water retention capacity and the solubility in water of the EPS were evaluated according to the method of Du et al. (2018), obtaining values of 445.35 ± 12.23 and 98.24 ± 0.61 %, respectively. The results of this study suggest that the EPS of Lg. salivarius CRL 2217, synthesized in an MRS medium with sucrose as the energy source, was secreted around the bacterium in a discontinuous manner and may be weakly bound to the cell surface. It exhibits a lattice structure that confers excellent solubility and water-holding capacity. This EPS could be a potential additive for use as a thickener, stabilizer, and emulsifier in farm animal feed products. Also, its potential in drug delivery could be explored.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/287767
Characterization of EPS from Ligilactobacillus salivarius CRL 2217 by transmission and scanning electron microscopy techniques; VIII Congreso Argentino de Microscopía; Tafí Viejo; Argentina; 2024; 138-139
CONICET Digital
CONICET
url http://hdl.handle.net/11336/287767
identifier_str_mv Characterization of EPS from Ligilactobacillus salivarius CRL 2217 by transmission and scanning electron microscopy techniques; VIII Congreso Argentino de Microscopía; Tafí Viejo; Argentina; 2024; 138-139
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.samictucuman2024.com/certificados
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Microscopía
publisher.none.fl_str_mv Sociedad Argentina de Microscopía
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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