Corynebacterium pseudodiphteriticum stimulate alveolar macrophages and improve the respiratory humoral immune response

Autores
Ortiz Moyano, Francisco Ramiro; Raya Tonetti, María Fernanda; Elean, Mariano Daniel; Dentice Maidana, Stefania; Albarracín, Leonardo Miguel; Alvarez, Gladis Susana; Villena, Julio Cesar
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Previously, we demonstrated that nasally administered Corynebacterium pseudodiphteriticum 090104 (Cp) or its bacterium-like particles (BLPCp) were able to increase the resistance of mice against bacterial and viral respiratory pathogens. In this work, we evaluated: a) the interaction of Cp and BLPCp with alveolar macrophages (AMs) and b) the ability of Cp and BLPCp to enhance the humoral immune response induced by a commercial vaccine against Streptococcus pneumoniae. In the first set of experiments, Cp or BLPCp were incubated with primary cultures of murine AMs and the phagocytic activity, and the production of cytokines was evaluated. Optical and electron microscopy analysis revealed that both Cp and BLPCp were efficiently phagocyted by AMs. Both treatments were also able to trigger the production of TNF-α, IFN-γ, IL-6 and IL-1β (p<0.05 vs non-stimulated controls) by AMs. In the second set of experiments, 3 weeks-old-Swiss mice were intranasally immunized at days 0, 14 and 28 with 1:100 PBS dilution of the pneumococcal vaccine Prevenar®13 (PCV), Cp+PCV or BLPCp+PCV, using doses of CP and BLPCp of 108 cells or particles/ml. On day 33, samples of bronco-alveolar lavages (BAL) and serum were collected for the study of specific antibodies. In addition, immunized mice were challenged with S. pneumoniae serotypes 6B or 19F (108 UFC/ml PBS) on day 33 and sacrificed on day 35 (day 2 post-infection) to evaluate the resistance to the infection. Both Cp+PCV and BLPCp+PCV groups had higher specific serum IgG and BAL IgA antibodies than PCV control mice (p<0.05). In addition, mice immunized with Cp+PCV or BLPCp+PCV had lower lung pneumococcal cell counts (p<0.05) as well as lower levels of BAL albumin and LDH indicating a reduced alteration of the alveolar-capillary barrier and lower cellular damage than control mice. The results demonstrated that C. pseudodiphteriticum 090104 and its bacterium-like particles are capable to stimulate the respiratory innate immune system serving as adjuvants to potentiate the adaptive humoral immune response. Our study is a step forward in the positioning of this respiratory commensal bacterium as a promising mucosal adjuvant for vaccine formulations aimed to combat respiratory infectious diseases.
Fil: Ortiz Moyano, Francisco Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Raya Tonetti, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Elean, Mariano Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Dentice Maidana, Stefania. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
LXVII Reunión anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología; III Congreso Franco-Argentino de Inmunología; Reunión Anual 2022 Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
Materia
CORYNEBACTERIUM PSEUDODIPHTERITICUM
BACTERIUM-LIKE PARTICLES
RESPIRATORY INFECTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/290146
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/290146
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Corynebacterium pseudodiphteriticum stimulate alveolar macrophages and improve the respiratory humoral immune responseOrtiz Moyano, Francisco RamiroRaya Tonetti, María FernandaElean, Mariano DanielDentice Maidana, StefaniaAlbarracín, Leonardo MiguelAlvarez, Gladis SusanaVillena, Julio CesarCORYNEBACTERIUM PSEUDODIPHTERITICUMBACTERIUM-LIKE PARTICLESRESPIRATORY INFECTIONhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Previously, we demonstrated that nasally administered Corynebacterium pseudodiphteriticum 090104 (Cp) or its bacterium-like particles (BLPCp) were able to increase the resistance of mice against bacterial and viral respiratory pathogens. In this work, we evaluated: a) the interaction of Cp and BLPCp with alveolar macrophages (AMs) and b) the ability of Cp and BLPCp to enhance the humoral immune response induced by a commercial vaccine against Streptococcus pneumoniae. In the first set of experiments, Cp or BLPCp were incubated with primary cultures of murine AMs and the phagocytic activity, and the production of cytokines was evaluated. Optical and electron microscopy analysis revealed that both Cp and BLPCp were efficiently phagocyted by AMs. Both treatments were also able to trigger the production of TNF-α, IFN-γ, IL-6 and IL-1β (p<0.05 vs non-stimulated controls) by AMs. In the second set of experiments, 3 weeks-old-Swiss mice were intranasally immunized at days 0, 14 and 28 with 1:100 PBS dilution of the pneumococcal vaccine Prevenar®13 (PCV), Cp+PCV or BLPCp+PCV, using doses of CP and BLPCp of 108 cells or particles/ml. On day 33, samples of bronco-alveolar lavages (BAL) and serum were collected for the study of specific antibodies. In addition, immunized mice were challenged with S. pneumoniae serotypes 6B or 19F (108 UFC/ml PBS) on day 33 and sacrificed on day 35 (day 2 post-infection) to evaluate the resistance to the infection. Both Cp+PCV and BLPCp+PCV groups had higher specific serum IgG and BAL IgA antibodies than PCV control mice (p<0.05). In addition, mice immunized with Cp+PCV or BLPCp+PCV had lower lung pneumococcal cell counts (p<0.05) as well as lower levels of BAL albumin and LDH indicating a reduced alteration of the alveolar-capillary barrier and lower cellular damage than control mice. The results demonstrated that C. pseudodiphteriticum 090104 and its bacterium-like particles are capable to stimulate the respiratory innate immune system serving as adjuvants to potentiate the adaptive humoral immune response. Our study is a step forward in the positioning of this respiratory commensal bacterium as a promising mucosal adjuvant for vaccine formulations aimed to combat respiratory infectious diseases.Fil: Ortiz Moyano, Francisco Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Raya Tonetti, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Elean, Mariano Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Dentice Maidana, Stefania. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaLXVII Reunión anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología; III Congreso Franco-Argentino de Inmunología; Reunión Anual 2022 Sociedad Argentina de FisiologíaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de InmunologíaSociedad Argentina de FisiologíaFundación Revista Medicina2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/290146Corynebacterium pseudodiphteriticum stimulate alveolar macrophages and improve the respiratory humoral immune response; LXVII Reunión anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología; III Congreso Franco-Argentino de Inmunología; Reunión Anual 2022 Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 205-2050025-76801669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol82-22/s5/1s5.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:33:02Zoai:ri.conicet.gov.ar:11336/290146instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:33:02.776CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Corynebacterium pseudodiphteriticum stimulate alveolar macrophages and improve the respiratory humoral immune response
title Corynebacterium pseudodiphteriticum stimulate alveolar macrophages and improve the respiratory humoral immune response
spellingShingle Corynebacterium pseudodiphteriticum stimulate alveolar macrophages and improve the respiratory humoral immune response
Ortiz Moyano, Francisco Ramiro
CORYNEBACTERIUM PSEUDODIPHTERITICUM
BACTERIUM-LIKE PARTICLES
RESPIRATORY INFECTION
title_short Corynebacterium pseudodiphteriticum stimulate alveolar macrophages and improve the respiratory humoral immune response
title_full Corynebacterium pseudodiphteriticum stimulate alveolar macrophages and improve the respiratory humoral immune response
title_fullStr Corynebacterium pseudodiphteriticum stimulate alveolar macrophages and improve the respiratory humoral immune response
title_full_unstemmed Corynebacterium pseudodiphteriticum stimulate alveolar macrophages and improve the respiratory humoral immune response
title_sort Corynebacterium pseudodiphteriticum stimulate alveolar macrophages and improve the respiratory humoral immune response
dc.creator.none.fl_str_mv Ortiz Moyano, Francisco Ramiro
Raya Tonetti, María Fernanda
Elean, Mariano Daniel
Dentice Maidana, Stefania
Albarracín, Leonardo Miguel
Alvarez, Gladis Susana
Villena, Julio Cesar
author Ortiz Moyano, Francisco Ramiro
author_facet Ortiz Moyano, Francisco Ramiro
Raya Tonetti, María Fernanda
Elean, Mariano Daniel
Dentice Maidana, Stefania
Albarracín, Leonardo Miguel
Alvarez, Gladis Susana
Villena, Julio Cesar
author_role author
author2 Raya Tonetti, María Fernanda
Elean, Mariano Daniel
Dentice Maidana, Stefania
Albarracín, Leonardo Miguel
Alvarez, Gladis Susana
Villena, Julio Cesar
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv CORYNEBACTERIUM PSEUDODIPHTERITICUM
BACTERIUM-LIKE PARTICLES
RESPIRATORY INFECTION
topic CORYNEBACTERIUM PSEUDODIPHTERITICUM
BACTERIUM-LIKE PARTICLES
RESPIRATORY INFECTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Previously, we demonstrated that nasally administered Corynebacterium pseudodiphteriticum 090104 (Cp) or its bacterium-like particles (BLPCp) were able to increase the resistance of mice against bacterial and viral respiratory pathogens. In this work, we evaluated: a) the interaction of Cp and BLPCp with alveolar macrophages (AMs) and b) the ability of Cp and BLPCp to enhance the humoral immune response induced by a commercial vaccine against Streptococcus pneumoniae. In the first set of experiments, Cp or BLPCp were incubated with primary cultures of murine AMs and the phagocytic activity, and the production of cytokines was evaluated. Optical and electron microscopy analysis revealed that both Cp and BLPCp were efficiently phagocyted by AMs. Both treatments were also able to trigger the production of TNF-α, IFN-γ, IL-6 and IL-1β (p<0.05 vs non-stimulated controls) by AMs. In the second set of experiments, 3 weeks-old-Swiss mice were intranasally immunized at days 0, 14 and 28 with 1:100 PBS dilution of the pneumococcal vaccine Prevenar®13 (PCV), Cp+PCV or BLPCp+PCV, using doses of CP and BLPCp of 108 cells or particles/ml. On day 33, samples of bronco-alveolar lavages (BAL) and serum were collected for the study of specific antibodies. In addition, immunized mice were challenged with S. pneumoniae serotypes 6B or 19F (108 UFC/ml PBS) on day 33 and sacrificed on day 35 (day 2 post-infection) to evaluate the resistance to the infection. Both Cp+PCV and BLPCp+PCV groups had higher specific serum IgG and BAL IgA antibodies than PCV control mice (p<0.05). In addition, mice immunized with Cp+PCV or BLPCp+PCV had lower lung pneumococcal cell counts (p<0.05) as well as lower levels of BAL albumin and LDH indicating a reduced alteration of the alveolar-capillary barrier and lower cellular damage than control mice. The results demonstrated that C. pseudodiphteriticum 090104 and its bacterium-like particles are capable to stimulate the respiratory innate immune system serving as adjuvants to potentiate the adaptive humoral immune response. Our study is a step forward in the positioning of this respiratory commensal bacterium as a promising mucosal adjuvant for vaccine formulations aimed to combat respiratory infectious diseases.
Fil: Ortiz Moyano, Francisco Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Raya Tonetti, María Fernanda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Elean, Mariano Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Dentice Maidana, Stefania. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Alvarez, Gladis Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
LXVII Reunión anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología; III Congreso Franco-Argentino de Inmunología; Reunión Anual 2022 Sociedad Argentina de Fisiología
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Inmunología
Sociedad Argentina de Fisiología
description Previously, we demonstrated that nasally administered Corynebacterium pseudodiphteriticum 090104 (Cp) or its bacterium-like particles (BLPCp) were able to increase the resistance of mice against bacterial and viral respiratory pathogens. In this work, we evaluated: a) the interaction of Cp and BLPCp with alveolar macrophages (AMs) and b) the ability of Cp and BLPCp to enhance the humoral immune response induced by a commercial vaccine against Streptococcus pneumoniae. In the first set of experiments, Cp or BLPCp were incubated with primary cultures of murine AMs and the phagocytic activity, and the production of cytokines was evaluated. Optical and electron microscopy analysis revealed that both Cp and BLPCp were efficiently phagocyted by AMs. Both treatments were also able to trigger the production of TNF-α, IFN-γ, IL-6 and IL-1β (p<0.05 vs non-stimulated controls) by AMs. In the second set of experiments, 3 weeks-old-Swiss mice were intranasally immunized at days 0, 14 and 28 with 1:100 PBS dilution of the pneumococcal vaccine Prevenar®13 (PCV), Cp+PCV or BLPCp+PCV, using doses of CP and BLPCp of 108 cells or particles/ml. On day 33, samples of bronco-alveolar lavages (BAL) and serum were collected for the study of specific antibodies. In addition, immunized mice were challenged with S. pneumoniae serotypes 6B or 19F (108 UFC/ml PBS) on day 33 and sacrificed on day 35 (day 2 post-infection) to evaluate the resistance to the infection. Both Cp+PCV and BLPCp+PCV groups had higher specific serum IgG and BAL IgA antibodies than PCV control mice (p<0.05). In addition, mice immunized with Cp+PCV or BLPCp+PCV had lower lung pneumococcal cell counts (p<0.05) as well as lower levels of BAL albumin and LDH indicating a reduced alteration of the alveolar-capillary barrier and lower cellular damage than control mice. The results demonstrated that C. pseudodiphteriticum 090104 and its bacterium-like particles are capable to stimulate the respiratory innate immune system serving as adjuvants to potentiate the adaptive humoral immune response. Our study is a step forward in the positioning of this respiratory commensal bacterium as a promising mucosal adjuvant for vaccine formulations aimed to combat respiratory infectious diseases.
publishDate 2022
dc.date.none.fl_str_mv 2022
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info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/290146
Corynebacterium pseudodiphteriticum stimulate alveolar macrophages and improve the respiratory humoral immune response; LXVII Reunión anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología; III Congreso Franco-Argentino de Inmunología; Reunión Anual 2022 Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 205-205
0025-7680
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/290146
identifier_str_mv Corynebacterium pseudodiphteriticum stimulate alveolar macrophages and improve the respiratory humoral immune response; LXVII Reunión anual de la Sociedad Argentina de Investigación Clínica; LXX Reunión Anual de la Sociedad Argentina de Inmunología; III Congreso Franco-Argentino de Inmunología; Reunión Anual 2022 Sociedad Argentina de Fisiología; Mar del Plata; Argentina; 2022; 205-205
0025-7680
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol82-22/s5/1s5.pdf
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dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
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repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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