Venomic and F(ab')₂ antivenomic studies of Bothrops alternatus venom proteins

Autores
Fusco, Luciano Sebastian; Beraldo Neto, Emidio; Calvete, Juan J.; Sanz, Libia; Lanari, Laura C.; Lorente, Carolina; Leiva, Laura C.; Pimenta, Daniel; de Roodt, Adolfo Rafael; Pla, Davinia
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, using a proteomic approach, we performed the venomic characterization of B. alternatus venomproteins. The venom comprises toxins from eleven protein families and the main identified included PIII-SVMP(27.32%), PII-SVMP (12.33%), Disintegrin-SVMP (10.26%), SVSP (8.80%), PLA2 (6.85%), BBP/C-NP (4.85%),CRVP (4.87%), CTL (6.80%), LAAO (4.14%), SVGF (1.90%), VPA (1.64%), PDE (0.07%), and an unidentifiedpercentage (11.64%). Antivenomic analysis showed that both F(ab’)₂ antivenoms recognized all venom proteinsbut had low affinity for small peptides like BPP. The BIOL antivenom demonstrated a binding capacity of 56.92mg/g F(ab’)2, while the INPB antivenom showed a significantly higher binding capacity of 124.78 mg/g F(ab’)2.These results align with in vivo studies, where the antivenom potency was 63.28 mg/g F(ab’)2 and 129.38 mg/gF(ab’)2 for BIOL and INPB, respectively. This study highlights the value of third-generation antivenomics incharacterizing antivenoms used against B. alternatus envenomation and confirms the effectiveness of these twoantivenoms in treating snakebites caused by B. alternatus. In conclusion, this study reports for the first time acomparative analysis of the venomics and antivenomics of two antivenoms used in Argentina for the treatment ofB. alternatus snake bites, integrating both in vitro and in vivo approaches.
Fil: Fusco, Luciano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Beraldo Neto, Emidio. No especifíca;
Fil: Calvete, Juan J.. Consejo Superior de Investigaciones Científicas; España
Fil: Sanz, Libia. Consejo Superior de Investigaciones Científicas; España
Fil: Lanari, Laura C.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina
Fil: Lorente, Carolina. Consejo Superior de Investigaciones Científicas; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Leiva, Laura C.. Universidad Nacional del Nordeste; Argentina
Fil: Pimenta, Daniel. No especifíca;
Fil: de Roodt, Adolfo Rafael. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina
Fil: Pla, Davinia. Consejo Superior de Investigaciones Científicas; España
Materia
Proteomic
Antivenomic
Ativenom
Snake venom
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/282791
Acceder
id CONICETDig_970820b6af62509dd88ad821ad412fc0
oai_identifier_str oai:ri.conicet.gov.ar:11336/282791
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Venomic and F(ab')₂ antivenomic studies of Bothrops alternatus venom proteinsFusco, Luciano SebastianBeraldo Neto, EmidioCalvete, Juan J.Sanz, LibiaLanari, Laura C.Lorente, CarolinaLeiva, Laura C.Pimenta, Danielde Roodt, Adolfo RafaelPla, DaviniaProteomicAntivenomicAtivenomSnake venomhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In this study, using a proteomic approach, we performed the venomic characterization of B. alternatus venomproteins. The venom comprises toxins from eleven protein families and the main identified included PIII-SVMP(27.32%), PII-SVMP (12.33%), Disintegrin-SVMP (10.26%), SVSP (8.80%), PLA2 (6.85%), BBP/C-NP (4.85%),CRVP (4.87%), CTL (6.80%), LAAO (4.14%), SVGF (1.90%), VPA (1.64%), PDE (0.07%), and an unidentifiedpercentage (11.64%). Antivenomic analysis showed that both F(ab’)₂ antivenoms recognized all venom proteinsbut had low affinity for small peptides like BPP. The BIOL antivenom demonstrated a binding capacity of 56.92mg/g F(ab’)2, while the INPB antivenom showed a significantly higher binding capacity of 124.78 mg/g F(ab’)2.These results align with in vivo studies, where the antivenom potency was 63.28 mg/g F(ab’)2 and 129.38 mg/gF(ab’)2 for BIOL and INPB, respectively. This study highlights the value of third-generation antivenomics incharacterizing antivenoms used against B. alternatus envenomation and confirms the effectiveness of these twoantivenoms in treating snakebites caused by B. alternatus. In conclusion, this study reports for the first time acomparative analysis of the venomics and antivenomics of two antivenoms used in Argentina for the treatment ofB. alternatus snake bites, integrating both in vitro and in vivo approaches.Fil: Fusco, Luciano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; ArgentinaFil: Beraldo Neto, Emidio. No especifíca;Fil: Calvete, Juan J.. Consejo Superior de Investigaciones Científicas; EspañaFil: Sanz, Libia. Consejo Superior de Investigaciones Científicas; EspañaFil: Lanari, Laura C.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; ArgentinaFil: Lorente, Carolina. Consejo Superior de Investigaciones Científicas; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Leiva, Laura C.. Universidad Nacional del Nordeste; ArgentinaFil: Pimenta, Daniel. No especifíca;Fil: de Roodt, Adolfo Rafael. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; ArgentinaFil: Pla, Davinia. Consejo Superior de Investigaciones Científicas; EspañaElsevier Science2025-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/282791Fusco, Luciano Sebastian; Beraldo Neto, Emidio; Calvete, Juan J.; Sanz, Libia; Lanari, Laura C.; et al.; Venomic and F(ab')₂ antivenomic studies of Bothrops alternatus venom proteins; Elsevier Science; International Journal of Biological Macromolecules; 318; 7-2025; 1-90141-8130CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0141813025053292info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2025.144777info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:39:56Zoai:ri.conicet.gov.ar:11336/282791instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:39:56.419CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Venomic and F(ab')₂ antivenomic studies of Bothrops alternatus venom proteins
title Venomic and F(ab')₂ antivenomic studies of Bothrops alternatus venom proteins
spellingShingle Venomic and F(ab')₂ antivenomic studies of Bothrops alternatus venom proteins
Fusco, Luciano Sebastian
Proteomic
Antivenomic
Ativenom
Snake venom
title_short Venomic and F(ab')₂ antivenomic studies of Bothrops alternatus venom proteins
title_full Venomic and F(ab')₂ antivenomic studies of Bothrops alternatus venom proteins
title_fullStr Venomic and F(ab')₂ antivenomic studies of Bothrops alternatus venom proteins
title_full_unstemmed Venomic and F(ab')₂ antivenomic studies of Bothrops alternatus venom proteins
title_sort Venomic and F(ab')₂ antivenomic studies of Bothrops alternatus venom proteins
dc.creator.none.fl_str_mv Fusco, Luciano Sebastian
Beraldo Neto, Emidio
Calvete, Juan J.
Sanz, Libia
Lanari, Laura C.
Lorente, Carolina
Leiva, Laura C.
Pimenta, Daniel
de Roodt, Adolfo Rafael
Pla, Davinia
author Fusco, Luciano Sebastian
author_facet Fusco, Luciano Sebastian
Beraldo Neto, Emidio
Calvete, Juan J.
Sanz, Libia
Lanari, Laura C.
Lorente, Carolina
Leiva, Laura C.
Pimenta, Daniel
de Roodt, Adolfo Rafael
Pla, Davinia
author_role author
author2 Beraldo Neto, Emidio
Calvete, Juan J.
Sanz, Libia
Lanari, Laura C.
Lorente, Carolina
Leiva, Laura C.
Pimenta, Daniel
de Roodt, Adolfo Rafael
Pla, Davinia
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Proteomic
Antivenomic
Ativenom
Snake venom
topic Proteomic
Antivenomic
Ativenom
Snake venom
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In this study, using a proteomic approach, we performed the venomic characterization of B. alternatus venomproteins. The venom comprises toxins from eleven protein families and the main identified included PIII-SVMP(27.32%), PII-SVMP (12.33%), Disintegrin-SVMP (10.26%), SVSP (8.80%), PLA2 (6.85%), BBP/C-NP (4.85%),CRVP (4.87%), CTL (6.80%), LAAO (4.14%), SVGF (1.90%), VPA (1.64%), PDE (0.07%), and an unidentifiedpercentage (11.64%). Antivenomic analysis showed that both F(ab’)₂ antivenoms recognized all venom proteinsbut had low affinity for small peptides like BPP. The BIOL antivenom demonstrated a binding capacity of 56.92mg/g F(ab’)2, while the INPB antivenom showed a significantly higher binding capacity of 124.78 mg/g F(ab’)2.These results align with in vivo studies, where the antivenom potency was 63.28 mg/g F(ab’)2 and 129.38 mg/gF(ab’)2 for BIOL and INPB, respectively. This study highlights the value of third-generation antivenomics incharacterizing antivenoms used against B. alternatus envenomation and confirms the effectiveness of these twoantivenoms in treating snakebites caused by B. alternatus. In conclusion, this study reports for the first time acomparative analysis of the venomics and antivenomics of two antivenoms used in Argentina for the treatment ofB. alternatus snake bites, integrating both in vitro and in vivo approaches.
Fil: Fusco, Luciano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
Fil: Beraldo Neto, Emidio. No especifíca;
Fil: Calvete, Juan J.. Consejo Superior de Investigaciones Científicas; España
Fil: Sanz, Libia. Consejo Superior de Investigaciones Científicas; España
Fil: Lanari, Laura C.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina
Fil: Lorente, Carolina. Consejo Superior de Investigaciones Científicas; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Leiva, Laura C.. Universidad Nacional del Nordeste; Argentina
Fil: Pimenta, Daniel. No especifíca;
Fil: de Roodt, Adolfo Rafael. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán"; Argentina
Fil: Pla, Davinia. Consejo Superior de Investigaciones Científicas; España
description In this study, using a proteomic approach, we performed the venomic characterization of B. alternatus venomproteins. The venom comprises toxins from eleven protein families and the main identified included PIII-SVMP(27.32%), PII-SVMP (12.33%), Disintegrin-SVMP (10.26%), SVSP (8.80%), PLA2 (6.85%), BBP/C-NP (4.85%),CRVP (4.87%), CTL (6.80%), LAAO (4.14%), SVGF (1.90%), VPA (1.64%), PDE (0.07%), and an unidentifiedpercentage (11.64%). Antivenomic analysis showed that both F(ab’)₂ antivenoms recognized all venom proteinsbut had low affinity for small peptides like BPP. The BIOL antivenom demonstrated a binding capacity of 56.92mg/g F(ab’)2, while the INPB antivenom showed a significantly higher binding capacity of 124.78 mg/g F(ab’)2.These results align with in vivo studies, where the antivenom potency was 63.28 mg/g F(ab’)2 and 129.38 mg/gF(ab’)2 for BIOL and INPB, respectively. This study highlights the value of third-generation antivenomics incharacterizing antivenoms used against B. alternatus envenomation and confirms the effectiveness of these twoantivenoms in treating snakebites caused by B. alternatus. In conclusion, this study reports for the first time acomparative analysis of the venomics and antivenomics of two antivenoms used in Argentina for the treatment ofB. alternatus snake bites, integrating both in vitro and in vivo approaches.
publishDate 2025
dc.date.none.fl_str_mv 2025-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/282791
Fusco, Luciano Sebastian; Beraldo Neto, Emidio; Calvete, Juan J.; Sanz, Libia; Lanari, Laura C.; et al.; Venomic and F(ab')₂ antivenomic studies of Bothrops alternatus venom proteins; Elsevier Science; International Journal of Biological Macromolecules; 318; 7-2025; 1-9
0141-8130
CONICET Digital
CONICET
url http://hdl.handle.net/11336/282791
identifier_str_mv Fusco, Luciano Sebastian; Beraldo Neto, Emidio; Calvete, Juan J.; Sanz, Libia; Lanari, Laura C.; et al.; Venomic and F(ab')₂ antivenomic studies of Bothrops alternatus venom proteins; Elsevier Science; International Journal of Biological Macromolecules; 318; 7-2025; 1-9
0141-8130
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0141813025053292
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ijbiomac.2025.144777
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.265058

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