Simultaneous ManNAc and Neu5Ac Quantification in Human Sera by LC-MS/MS

Autores
Guerrero Flores, Gerardo Nataniel; Pacheco, Fabio Juliano; Martinez Marignac, Veronica Lucrecia; Perry, Christopher; Zhang, Guangyu; Mayta, Martín Leonardo; Voglmeir, Josef; Liu, Li; Fraser, Gary E.; Miles Butler, Fayth L.; Boskovic, Danilo S.
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
N-Acetyl-D-mannosamine (ManNAc) and N-acetylneuraminic acid (Neu5Ac) are important components of glycosylation, affecting numerous physiologic processes. The effects of age, body mass index (BMI), race, or sex on serum levels of ManNAc and Neu5Ac are poorly understood. However, these associations are of substantial interest. Simultaneous quantification of ManNAc and Neu5Ac, using liquid chromatography tandem mass spectrometry (LC-MS/MS), was developed and validated for human serum samples. This method has high sensitivity, specificity, and reproducibility, with limits of detection as low as 1.02 ng/mL for ManNAc or 1.14 ng/mL for Neu5Ac. A set of 155 serum samples from the Adventist Health Study 2 (AHS-2) cohort was analyzed. Concentrations of conjugated Neu5Ac were 35.1 ± 9.4 µg/mL and 33.0 ± 9.5 µg/mL in black and white participants, respectively. Conjugated and total Neu5Ac levels were significantly higher in women, with p-values of 0.029 and 0.026, respectively. The free forms of Neu5Ac were 594 ± 421 ng/mL and 439 ± 168 ng/mL in black and white participants, respectively. Similarly, conjugated and total ManNAc levels were higher in black participants, at 1.81 ± 0.81 µg/mL and 1.90 ± 0.83 µg/mL, compared to 1.32 ± 0.52 µg/mL and 1.41 ± 0.53 µg/mL in white participants (both cases, p < 0.001). Free ManNAc was 93.1 ± 36.2 ng/mL in black and 89 ± 20.2 ng/mL in white participants. Subjects with higher BMI tend to have higher free ManNAc (p = 0.041). Furthermore, older subjects tend to have higher free (p ≤ 0.001) and total (p = 0.045) ManNAc. The improved LC-MS/MS quantification method should facilitate further investigations.
Fil: Guerrero Flores, Gerardo Nataniel. Universidad Adventista del Plata. Secretaría de Ciencia y Técnica. Centro Interdisciplinario de Investigaciones en Ciencias de la Salud y del Comportamiento; Argentina. Universidad Nacional de Rosario; Argentina
Fil: Pacheco, Fabio Juliano. Universidad Adventista del Plata. Secretaría de Ciencia y Técnica. Centro Interdisciplinario de Investigaciones en Ciencias de la Salud y del Comportamiento; Argentina
Fil: Martinez Marignac, Veronica Lucrecia. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina
Fil: Perry, Christopher. Loma Linda University (llu);
Fil: Zhang, Guangyu. Loma Linda University (llu);
Fil: Mayta, Martín Leonardo. Universidad Adventista del Plata. Secretaría de Ciencia y Técnica. Centro Interdisciplinario de Investigaciones en Ciencias de la Salud y del Comportamiento; Argentina. Universidad Nacional de Rosario; Argentina
Fil: Voglmeir, Josef. Nanjing Agricultural University; China
Fil: Liu, Li. Nanjing Agricultural University; China
Fil: Fraser, Gary E.. Loma Linda University (llu);
Fil: Miles Butler, Fayth L.. Loma Linda University (llu);
Fil: Boskovic, Danilo S.. Loma Linda University (llu);
Materia
Neu5Ac
ManNAC
Human sera
LC-MS/MS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/286257
Acceder
id CONICETDig_9d0c1e9a6eea177215b52430ccc4edba
oai_identifier_str oai:ri.conicet.gov.ar:11336/286257
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Simultaneous ManNAc and Neu5Ac Quantification in Human Sera by LC-MS/MSGuerrero Flores, Gerardo NatanielPacheco, Fabio JulianoMartinez Marignac, Veronica LucreciaPerry, ChristopherZhang, GuangyuMayta, Martín LeonardoVoglmeir, JosefLiu, LiFraser, Gary E.Miles Butler, Fayth L.Boskovic, Danilo S.Neu5AcManNACHuman seraLC-MS/MShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3N-Acetyl-D-mannosamine (ManNAc) and N-acetylneuraminic acid (Neu5Ac) are important components of glycosylation, affecting numerous physiologic processes. The effects of age, body mass index (BMI), race, or sex on serum levels of ManNAc and Neu5Ac are poorly understood. However, these associations are of substantial interest. Simultaneous quantification of ManNAc and Neu5Ac, using liquid chromatography tandem mass spectrometry (LC-MS/MS), was developed and validated for human serum samples. This method has high sensitivity, specificity, and reproducibility, with limits of detection as low as 1.02 ng/mL for ManNAc or 1.14 ng/mL for Neu5Ac. A set of 155 serum samples from the Adventist Health Study 2 (AHS-2) cohort was analyzed. Concentrations of conjugated Neu5Ac were 35.1 ± 9.4 µg/mL and 33.0 ± 9.5 µg/mL in black and white participants, respectively. Conjugated and total Neu5Ac levels were significantly higher in women, with p-values of 0.029 and 0.026, respectively. The free forms of Neu5Ac were 594 ± 421 ng/mL and 439 ± 168 ng/mL in black and white participants, respectively. Similarly, conjugated and total ManNAc levels were higher in black participants, at 1.81 ± 0.81 µg/mL and 1.90 ± 0.83 µg/mL, compared to 1.32 ± 0.52 µg/mL and 1.41 ± 0.53 µg/mL in white participants (both cases, p < 0.001). Free ManNAc was 93.1 ± 36.2 ng/mL in black and 89 ± 20.2 ng/mL in white participants. Subjects with higher BMI tend to have higher free ManNAc (p = 0.041). Furthermore, older subjects tend to have higher free (p ≤ 0.001) and total (p = 0.045) ManNAc. The improved LC-MS/MS quantification method should facilitate further investigations.Fil: Guerrero Flores, Gerardo Nataniel. Universidad Adventista del Plata. Secretaría de Ciencia y Técnica. Centro Interdisciplinario de Investigaciones en Ciencias de la Salud y del Comportamiento; Argentina. Universidad Nacional de Rosario; ArgentinaFil: Pacheco, Fabio Juliano. Universidad Adventista del Plata. Secretaría de Ciencia y Técnica. Centro Interdisciplinario de Investigaciones en Ciencias de la Salud y del Comportamiento; ArgentinaFil: Martinez Marignac, Veronica Lucrecia. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; ArgentinaFil: Perry, Christopher. Loma Linda University (llu);Fil: Zhang, Guangyu. Loma Linda University (llu);Fil: Mayta, Martín Leonardo. Universidad Adventista del Plata. Secretaría de Ciencia y Técnica. Centro Interdisciplinario de Investigaciones en Ciencias de la Salud y del Comportamiento; Argentina. Universidad Nacional de Rosario; ArgentinaFil: Voglmeir, Josef. Nanjing Agricultural University; ChinaFil: Liu, Li. Nanjing Agricultural University; ChinaFil: Fraser, Gary E.. Loma Linda University (llu);Fil: Miles Butler, Fayth L.. Loma Linda University (llu);Fil: Boskovic, Danilo S.. Loma Linda University (llu);Multidisciplinary Digital Publishing Institute2026-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/286257Guerrero Flores, Gerardo Nataniel; Pacheco, Fabio Juliano; Martinez Marignac, Veronica Lucrecia; Perry, Christopher; Zhang, Guangyu; et al.; Simultaneous ManNAc and Neu5Ac Quantification in Human Sera by LC-MS/MS; Multidisciplinary Digital Publishing Institute; International Journal of Molecular Sciences; 27; 894; 1-2026; 1-251422-0067CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/41596540/info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms27020894info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/27/2/894info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:32:06Zoai:ri.conicet.gov.ar:11336/286257instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:32:06.832CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Simultaneous ManNAc and Neu5Ac Quantification in Human Sera by LC-MS/MS
title Simultaneous ManNAc and Neu5Ac Quantification in Human Sera by LC-MS/MS
spellingShingle Simultaneous ManNAc and Neu5Ac Quantification in Human Sera by LC-MS/MS
Guerrero Flores, Gerardo Nataniel
Neu5Ac
ManNAC
Human sera
LC-MS/MS
title_short Simultaneous ManNAc and Neu5Ac Quantification in Human Sera by LC-MS/MS
title_full Simultaneous ManNAc and Neu5Ac Quantification in Human Sera by LC-MS/MS
title_fullStr Simultaneous ManNAc and Neu5Ac Quantification in Human Sera by LC-MS/MS
title_full_unstemmed Simultaneous ManNAc and Neu5Ac Quantification in Human Sera by LC-MS/MS
title_sort Simultaneous ManNAc and Neu5Ac Quantification in Human Sera by LC-MS/MS
dc.creator.none.fl_str_mv Guerrero Flores, Gerardo Nataniel
Pacheco, Fabio Juliano
Martinez Marignac, Veronica Lucrecia
Perry, Christopher
Zhang, Guangyu
Mayta, Martín Leonardo
Voglmeir, Josef
Liu, Li
Fraser, Gary E.
Miles Butler, Fayth L.
Boskovic, Danilo S.
author Guerrero Flores, Gerardo Nataniel
author_facet Guerrero Flores, Gerardo Nataniel
Pacheco, Fabio Juliano
Martinez Marignac, Veronica Lucrecia
Perry, Christopher
Zhang, Guangyu
Mayta, Martín Leonardo
Voglmeir, Josef
Liu, Li
Fraser, Gary E.
Miles Butler, Fayth L.
Boskovic, Danilo S.
author_role author
author2 Pacheco, Fabio Juliano
Martinez Marignac, Veronica Lucrecia
Perry, Christopher
Zhang, Guangyu
Mayta, Martín Leonardo
Voglmeir, Josef
Liu, Li
Fraser, Gary E.
Miles Butler, Fayth L.
Boskovic, Danilo S.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Neu5Ac
ManNAC
Human sera
LC-MS/MS
topic Neu5Ac
ManNAC
Human sera
LC-MS/MS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv N-Acetyl-D-mannosamine (ManNAc) and N-acetylneuraminic acid (Neu5Ac) are important components of glycosylation, affecting numerous physiologic processes. The effects of age, body mass index (BMI), race, or sex on serum levels of ManNAc and Neu5Ac are poorly understood. However, these associations are of substantial interest. Simultaneous quantification of ManNAc and Neu5Ac, using liquid chromatography tandem mass spectrometry (LC-MS/MS), was developed and validated for human serum samples. This method has high sensitivity, specificity, and reproducibility, with limits of detection as low as 1.02 ng/mL for ManNAc or 1.14 ng/mL for Neu5Ac. A set of 155 serum samples from the Adventist Health Study 2 (AHS-2) cohort was analyzed. Concentrations of conjugated Neu5Ac were 35.1 ± 9.4 µg/mL and 33.0 ± 9.5 µg/mL in black and white participants, respectively. Conjugated and total Neu5Ac levels were significantly higher in women, with p-values of 0.029 and 0.026, respectively. The free forms of Neu5Ac were 594 ± 421 ng/mL and 439 ± 168 ng/mL in black and white participants, respectively. Similarly, conjugated and total ManNAc levels were higher in black participants, at 1.81 ± 0.81 µg/mL and 1.90 ± 0.83 µg/mL, compared to 1.32 ± 0.52 µg/mL and 1.41 ± 0.53 µg/mL in white participants (both cases, p < 0.001). Free ManNAc was 93.1 ± 36.2 ng/mL in black and 89 ± 20.2 ng/mL in white participants. Subjects with higher BMI tend to have higher free ManNAc (p = 0.041). Furthermore, older subjects tend to have higher free (p ≤ 0.001) and total (p = 0.045) ManNAc. The improved LC-MS/MS quantification method should facilitate further investigations.
Fil: Guerrero Flores, Gerardo Nataniel. Universidad Adventista del Plata. Secretaría de Ciencia y Técnica. Centro Interdisciplinario de Investigaciones en Ciencias de la Salud y del Comportamiento; Argentina. Universidad Nacional de Rosario; Argentina
Fil: Pacheco, Fabio Juliano. Universidad Adventista del Plata. Secretaría de Ciencia y Técnica. Centro Interdisciplinario de Investigaciones en Ciencias de la Salud y del Comportamiento; Argentina
Fil: Martinez Marignac, Veronica Lucrecia. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina
Fil: Perry, Christopher. Loma Linda University (llu);
Fil: Zhang, Guangyu. Loma Linda University (llu);
Fil: Mayta, Martín Leonardo. Universidad Adventista del Plata. Secretaría de Ciencia y Técnica. Centro Interdisciplinario de Investigaciones en Ciencias de la Salud y del Comportamiento; Argentina. Universidad Nacional de Rosario; Argentina
Fil: Voglmeir, Josef. Nanjing Agricultural University; China
Fil: Liu, Li. Nanjing Agricultural University; China
Fil: Fraser, Gary E.. Loma Linda University (llu);
Fil: Miles Butler, Fayth L.. Loma Linda University (llu);
Fil: Boskovic, Danilo S.. Loma Linda University (llu);
description N-Acetyl-D-mannosamine (ManNAc) and N-acetylneuraminic acid (Neu5Ac) are important components of glycosylation, affecting numerous physiologic processes. The effects of age, body mass index (BMI), race, or sex on serum levels of ManNAc and Neu5Ac are poorly understood. However, these associations are of substantial interest. Simultaneous quantification of ManNAc and Neu5Ac, using liquid chromatography tandem mass spectrometry (LC-MS/MS), was developed and validated for human serum samples. This method has high sensitivity, specificity, and reproducibility, with limits of detection as low as 1.02 ng/mL for ManNAc or 1.14 ng/mL for Neu5Ac. A set of 155 serum samples from the Adventist Health Study 2 (AHS-2) cohort was analyzed. Concentrations of conjugated Neu5Ac were 35.1 ± 9.4 µg/mL and 33.0 ± 9.5 µg/mL in black and white participants, respectively. Conjugated and total Neu5Ac levels were significantly higher in women, with p-values of 0.029 and 0.026, respectively. The free forms of Neu5Ac were 594 ± 421 ng/mL and 439 ± 168 ng/mL in black and white participants, respectively. Similarly, conjugated and total ManNAc levels were higher in black participants, at 1.81 ± 0.81 µg/mL and 1.90 ± 0.83 µg/mL, compared to 1.32 ± 0.52 µg/mL and 1.41 ± 0.53 µg/mL in white participants (both cases, p < 0.001). Free ManNAc was 93.1 ± 36.2 ng/mL in black and 89 ± 20.2 ng/mL in white participants. Subjects with higher BMI tend to have higher free ManNAc (p = 0.041). Furthermore, older subjects tend to have higher free (p ≤ 0.001) and total (p = 0.045) ManNAc. The improved LC-MS/MS quantification method should facilitate further investigations.
publishDate 2026
dc.date.none.fl_str_mv 2026-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/286257
Guerrero Flores, Gerardo Nataniel; Pacheco, Fabio Juliano; Martinez Marignac, Veronica Lucrecia; Perry, Christopher; Zhang, Guangyu; et al.; Simultaneous ManNAc and Neu5Ac Quantification in Human Sera by LC-MS/MS; Multidisciplinary Digital Publishing Institute; International Journal of Molecular Sciences; 27; 894; 1-2026; 1-25
1422-0067
CONICET Digital
CONICET
url http://hdl.handle.net/11336/286257
identifier_str_mv Guerrero Flores, Gerardo Nataniel; Pacheco, Fabio Juliano; Martinez Marignac, Veronica Lucrecia; Perry, Christopher; Zhang, Guangyu; et al.; Simultaneous ManNAc and Neu5Ac Quantification in Human Sera by LC-MS/MS; Multidisciplinary Digital Publishing Institute; International Journal of Molecular Sciences; 27; 894; 1-2026; 1-25
1422-0067
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubmed.ncbi.nlm.nih.gov/41596540/
info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms27020894
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/27/2/894
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1874774099298877440
score 13.24418

Publicaciones similares