Self-assembled supramolecular nanogels based on poly(vinyl alcohol) and gallic acid as nanocarriers for breast cancer treatment

Autores
Rosso, Anabella Patricia; Macchione, Micaela Alejandra; Racca, Ana Cristina; Picchio, Matías Luis; Coronado, Eduardo A.; Martinelli, Marisa; García Schejtman, Sergio David
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A series of polyvinyl alcohol (PVA)-based materials cross-linked with varying amounts of gallic acid (GA) were synthesized using a simple one-pot methodology. Two protocols were developed to obtain microgels and nanogels, resulting in materials with sizes ranging from 800 to 1200 nm for microgels and from 180 to 400 nm for nanogels, the latter achieved through the incorporation of ultrasonication into the self-assembly process. The amount of GA was found to have a crucial role on the size of the micro/ nanogels, leading to particles of smaller size as the GA concentration increases for microgels, while quite the opposite trend was observed for nanogels. This behavior is attributed to the particle formation kinetics, leading to different structural arrangements. The different nano/microgels synthesized were characterized using DLS, TEM, FT-IR, and NMR. It was found that the synthesis performed under ultrasonication with 20 % GA gives rise to nanogels depicting the most favorable characteristics suitable for drug delivery applications, such as small sizes with a narrow distribution. Therefore, we used these nanogels as a prototype model for breast cancer treatment by encapsulating methylene blue (MB) and performing kinetics experiments of drug release at physiological (pH=7.4) and tumoral (pH=4.0) pH levels, along with cell viability and cytotoxicity assays. The selected NGs were found to be optimal nanocarriers for breast cancer treatment in vitro due to their suitable size [(173 ± 22) nm], excellent MB encapsulation efficiency(EE%) of up to 78 %, and controlled, pH-dependent release over time, with a higher release observed at pH 4. Furthermore, the MB-loaded system demonstrated enhanced drug efficacy and dark cytotoxicity against breast cancer cells in shorter periods.
Fil: Rosso, Anabella Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Macchione, Micaela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina
Fil: Racca, Ana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Picchio, Matías Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnologica Nacional. Facultad Regional Villa Maria; Argentina
Fil: Coronado, Eduardo A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Martinelli, Marisa. Universidad Nacional de Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada; Argentina
Fil: García Schejtman, Sergio David. Universidad Nacional de Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada; Argentina
Materia
Self-assembly
Supramolecular
Nanomedicine
Nanocarrier
PVA
Gallic acid
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/290707
Acceder
id CONICETDig_9d6dbf5ec112008766ebedc9b262f47f
oai_identifier_str oai:ri.conicet.gov.ar:11336/290707
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Self-assembled supramolecular nanogels based on poly(vinyl alcohol) and gallic acid as nanocarriers for breast cancer treatmentRosso, Anabella PatriciaMacchione, Micaela AlejandraRacca, Ana CristinaPicchio, Matías LuisCoronado, Eduardo A.Martinelli, MarisaGarcía Schejtman, Sergio DavidSelf-assemblySupramolecularNanomedicineNanocarrierPVAGallic acidhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3A series of polyvinyl alcohol (PVA)-based materials cross-linked with varying amounts of gallic acid (GA) were synthesized using a simple one-pot methodology. Two protocols were developed to obtain microgels and nanogels, resulting in materials with sizes ranging from 800 to 1200 nm for microgels and from 180 to 400 nm for nanogels, the latter achieved through the incorporation of ultrasonication into the self-assembly process. The amount of GA was found to have a crucial role on the size of the micro/ nanogels, leading to particles of smaller size as the GA concentration increases for microgels, while quite the opposite trend was observed for nanogels. This behavior is attributed to the particle formation kinetics, leading to different structural arrangements. The different nano/microgels synthesized were characterized using DLS, TEM, FT-IR, and NMR. It was found that the synthesis performed under ultrasonication with 20 % GA gives rise to nanogels depicting the most favorable characteristics suitable for drug delivery applications, such as small sizes with a narrow distribution. Therefore, we used these nanogels as a prototype model for breast cancer treatment by encapsulating methylene blue (MB) and performing kinetics experiments of drug release at physiological (pH=7.4) and tumoral (pH=4.0) pH levels, along with cell viability and cytotoxicity assays. The selected NGs were found to be optimal nanocarriers for breast cancer treatment in vitro due to their suitable size [(173 ± 22) nm], excellent MB encapsulation efficiency(EE%) of up to 78 %, and controlled, pH-dependent release over time, with a higher release observed at pH 4. Furthermore, the MB-loaded system demonstrated enhanced drug efficacy and dark cytotoxicity against breast cancer cells in shorter periods.Fil: Rosso, Anabella Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Macchione, Micaela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; ArgentinaFil: Racca, Ana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Picchio, Matías Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnologica Nacional. Facultad Regional Villa Maria; ArgentinaFil: Coronado, Eduardo A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Martinelli, Marisa. Universidad Nacional de Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada; ArgentinaFil: García Schejtman, Sergio David. Universidad Nacional de Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada; ArgentinaElsevier2025-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/290707Rosso, Anabella Patricia; Macchione, Micaela Alejandra; Racca, Ana Cristina; Picchio, Matías Luis; Coronado, Eduardo A.; et al.; Self-assembled supramolecular nanogels based on poly(vinyl alcohol) and gallic acid as nanocarriers for breast cancer treatment; Elsevier; Next Materials; 8; 7-2025; 100835-1008422949-8228CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2949822825003533info:eu-repo/semantics/altIdentifier/doi/10.1016/j.nxmate.2025.100835info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T15:27:52Zoai:ri.conicet.gov.ar:11336/290707instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 15:27:52.396CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Self-assembled supramolecular nanogels based on poly(vinyl alcohol) and gallic acid as nanocarriers for breast cancer treatment
title Self-assembled supramolecular nanogels based on poly(vinyl alcohol) and gallic acid as nanocarriers for breast cancer treatment
spellingShingle Self-assembled supramolecular nanogels based on poly(vinyl alcohol) and gallic acid as nanocarriers for breast cancer treatment
Rosso, Anabella Patricia
Self-assembly
Supramolecular
Nanomedicine
Nanocarrier
PVA
Gallic acid
title_short Self-assembled supramolecular nanogels based on poly(vinyl alcohol) and gallic acid as nanocarriers for breast cancer treatment
title_full Self-assembled supramolecular nanogels based on poly(vinyl alcohol) and gallic acid as nanocarriers for breast cancer treatment
title_fullStr Self-assembled supramolecular nanogels based on poly(vinyl alcohol) and gallic acid as nanocarriers for breast cancer treatment
title_full_unstemmed Self-assembled supramolecular nanogels based on poly(vinyl alcohol) and gallic acid as nanocarriers for breast cancer treatment
title_sort Self-assembled supramolecular nanogels based on poly(vinyl alcohol) and gallic acid as nanocarriers for breast cancer treatment
dc.creator.none.fl_str_mv Rosso, Anabella Patricia
Macchione, Micaela Alejandra
Racca, Ana Cristina
Picchio, Matías Luis
Coronado, Eduardo A.
Martinelli, Marisa
García Schejtman, Sergio David
author Rosso, Anabella Patricia
author_facet Rosso, Anabella Patricia
Macchione, Micaela Alejandra
Racca, Ana Cristina
Picchio, Matías Luis
Coronado, Eduardo A.
Martinelli, Marisa
García Schejtman, Sergio David
author_role author
author2 Macchione, Micaela Alejandra
Racca, Ana Cristina
Picchio, Matías Luis
Coronado, Eduardo A.
Martinelli, Marisa
García Schejtman, Sergio David
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Self-assembly
Supramolecular
Nanomedicine
Nanocarrier
PVA
Gallic acid
topic Self-assembly
Supramolecular
Nanomedicine
Nanocarrier
PVA
Gallic acid
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv A series of polyvinyl alcohol (PVA)-based materials cross-linked with varying amounts of gallic acid (GA) were synthesized using a simple one-pot methodology. Two protocols were developed to obtain microgels and nanogels, resulting in materials with sizes ranging from 800 to 1200 nm for microgels and from 180 to 400 nm for nanogels, the latter achieved through the incorporation of ultrasonication into the self-assembly process. The amount of GA was found to have a crucial role on the size of the micro/ nanogels, leading to particles of smaller size as the GA concentration increases for microgels, while quite the opposite trend was observed for nanogels. This behavior is attributed to the particle formation kinetics, leading to different structural arrangements. The different nano/microgels synthesized were characterized using DLS, TEM, FT-IR, and NMR. It was found that the synthesis performed under ultrasonication with 20 % GA gives rise to nanogels depicting the most favorable characteristics suitable for drug delivery applications, such as small sizes with a narrow distribution. Therefore, we used these nanogels as a prototype model for breast cancer treatment by encapsulating methylene blue (MB) and performing kinetics experiments of drug release at physiological (pH=7.4) and tumoral (pH=4.0) pH levels, along with cell viability and cytotoxicity assays. The selected NGs were found to be optimal nanocarriers for breast cancer treatment in vitro due to their suitable size [(173 ± 22) nm], excellent MB encapsulation efficiency(EE%) of up to 78 %, and controlled, pH-dependent release over time, with a higher release observed at pH 4. Furthermore, the MB-loaded system demonstrated enhanced drug efficacy and dark cytotoxicity against breast cancer cells in shorter periods.
Fil: Rosso, Anabella Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Macchione, Micaela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina
Fil: Racca, Ana Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Picchio, Matías Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnologica Nacional. Facultad Regional Villa Maria; Argentina
Fil: Coronado, Eduardo A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentina
Fil: Martinelli, Marisa. Universidad Nacional de Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada; Argentina
Fil: García Schejtman, Sergio David. Universidad Nacional de Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación y Desarrollo en Ingeniería de Procesos y Química Aplicada; Argentina
description A series of polyvinyl alcohol (PVA)-based materials cross-linked with varying amounts of gallic acid (GA) were synthesized using a simple one-pot methodology. Two protocols were developed to obtain microgels and nanogels, resulting in materials with sizes ranging from 800 to 1200 nm for microgels and from 180 to 400 nm for nanogels, the latter achieved through the incorporation of ultrasonication into the self-assembly process. The amount of GA was found to have a crucial role on the size of the micro/ nanogels, leading to particles of smaller size as the GA concentration increases for microgels, while quite the opposite trend was observed for nanogels. This behavior is attributed to the particle formation kinetics, leading to different structural arrangements. The different nano/microgels synthesized were characterized using DLS, TEM, FT-IR, and NMR. It was found that the synthesis performed under ultrasonication with 20 % GA gives rise to nanogels depicting the most favorable characteristics suitable for drug delivery applications, such as small sizes with a narrow distribution. Therefore, we used these nanogels as a prototype model for breast cancer treatment by encapsulating methylene blue (MB) and performing kinetics experiments of drug release at physiological (pH=7.4) and tumoral (pH=4.0) pH levels, along with cell viability and cytotoxicity assays. The selected NGs were found to be optimal nanocarriers for breast cancer treatment in vitro due to their suitable size [(173 ± 22) nm], excellent MB encapsulation efficiency(EE%) of up to 78 %, and controlled, pH-dependent release over time, with a higher release observed at pH 4. Furthermore, the MB-loaded system demonstrated enhanced drug efficacy and dark cytotoxicity against breast cancer cells in shorter periods.
publishDate 2025
dc.date.none.fl_str_mv 2025-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/290707
Rosso, Anabella Patricia; Macchione, Micaela Alejandra; Racca, Ana Cristina; Picchio, Matías Luis; Coronado, Eduardo A.; et al.; Self-assembled supramolecular nanogels based on poly(vinyl alcohol) and gallic acid as nanocarriers for breast cancer treatment; Elsevier; Next Materials; 8; 7-2025; 100835-100842
2949-8228
CONICET Digital
CONICET
url http://hdl.handle.net/11336/290707
identifier_str_mv Rosso, Anabella Patricia; Macchione, Micaela Alejandra; Racca, Ana Cristina; Picchio, Matías Luis; Coronado, Eduardo A.; et al.; Self-assembled supramolecular nanogels based on poly(vinyl alcohol) and gallic acid as nanocarriers for breast cancer treatment; Elsevier; Next Materials; 8; 7-2025; 100835-100842
2949-8228
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2949822825003533
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.nxmate.2025.100835
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24418

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