Growth hormone receptors in zebrafish (Danio rerio): Adult and embryonic expression patterns

Autores
Di Prinzio, Cecilia Mayra; Botta, Pablo Eduardo; Barriga, Elías H.; Ríos, Eduardo A.; Reyes, Ariel E.; Arranz, Silvia Eda
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Growth hormone receptor (GHR) is a critical regulator of growth and metabolism. Although two GHRs have been characterized in many fish species, their functional characteristics, mechanisms of regulation and roles in embryonic development remain unclear. The zebrafish (Danio rerio) is an excellent model organism to study both developmental and physiological processes. In the present work, we characterized the complete cDNA sequences of zebrafish GHRs, ghra and ghrb, and their gene structures. We studied the expression of both receptors in adult tissues, and during embryonic development and larval stages by means of RT-PCR and whole-mount in situ hybridization. We determined that both transcripts are maternal ones, with specific expression patterns during development. Both GHR transcripts are mainly expressed in the notochord, myotomes, anterior structures and in the yolk cell. Interestingly, their expression became undetectable at 96 h post- Fertilization. Unlike other reports in fish, ghrs expression could not be detected in brain when adult tissues were used, and we detected ghrb but not ghra transcripts in muscle. In addition, we determined alternative transcript sequences for ghra with specific domain deletions, and alternative transcripts for ghrb that generate a premature stop codon and codify for truncated isoforms. These isoforms lack intracellular regions necessary for the activation of signal transducers and activators of transcription (STAT) family transcription factors 5.
Fil: Di Prinzio, Cecilia Mayra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Botta, Pablo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Barriga, Elías H.. Universidad Andrés Bello; Chile
Fil: Ríos, Eduardo A.. Universidad Andrés Bello; Chile
Fil: Reyes, Ariel E.. Universidad Andrés Bello; Chile. Universidad Diego Portales; Chile
Fil: Arranz, Silvia Eda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Materia
ZEBRAFISH
GROWTH HORMONE RECEPTOR
GROWTH HORMONE
DEVELOPMENT
ALTERNATIVE TRANSCRIPTS
IN SITU HYBRIDIZATION
YOLK CELL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/284333
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/284333
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Growth hormone receptors in zebrafish (Danio rerio): Adult and embryonic expression patternsDi Prinzio, Cecilia MayraBotta, Pablo EduardoBarriga, Elías H.Ríos, Eduardo A.Reyes, Ariel E.Arranz, Silvia EdaZEBRAFISHGROWTH HORMONE RECEPTORGROWTH HORMONEDEVELOPMENTALTERNATIVE TRANSCRIPTSIN SITU HYBRIDIZATIONYOLK CELLhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Growth hormone receptor (GHR) is a critical regulator of growth and metabolism. Although two GHRs have been characterized in many fish species, their functional characteristics, mechanisms of regulation and roles in embryonic development remain unclear. The zebrafish (Danio rerio) is an excellent model organism to study both developmental and physiological processes. In the present work, we characterized the complete cDNA sequences of zebrafish GHRs, ghra and ghrb, and their gene structures. We studied the expression of both receptors in adult tissues, and during embryonic development and larval stages by means of RT-PCR and whole-mount in situ hybridization. We determined that both transcripts are maternal ones, with specific expression patterns during development. Both GHR transcripts are mainly expressed in the notochord, myotomes, anterior structures and in the yolk cell. Interestingly, their expression became undetectable at 96 h post- Fertilization. Unlike other reports in fish, ghrs expression could not be detected in brain when adult tissues were used, and we detected ghrb but not ghra transcripts in muscle. In addition, we determined alternative transcript sequences for ghra with specific domain deletions, and alternative transcripts for ghrb that generate a premature stop codon and codify for truncated isoforms. These isoforms lack intracellular regions necessary for the activation of signal transducers and activators of transcription (STAT) family transcription factors 5.Fil: Di Prinzio, Cecilia Mayra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Botta, Pablo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Barriga, Elías H.. Universidad Andrés Bello; ChileFil: Ríos, Eduardo A.. Universidad Andrés Bello; ChileFil: Reyes, Ariel E.. Universidad Andrés Bello; Chile. Universidad Diego Portales; ChileFil: Arranz, Silvia Eda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaElsevier Science2010-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/284333Di Prinzio, Cecilia Mayra; Botta, Pablo Eduardo; Barriga, Elías H.; Ríos, Eduardo A.; Reyes, Ariel E.; et al.; Growth hormone receptors in zebrafish (Danio rerio): Adult and embryonic expression patterns; Elsevier Science; Gene Expression Patterns; 10; 4-5; 6-2010; 214-2251567-133XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1567133X10000268info:eu-repo/semantics/altIdentifier/doi/10.1016/j.gep.2010.03.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:35:53Zoai:ri.conicet.gov.ar:11336/284333instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:35:54.049CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Growth hormone receptors in zebrafish (Danio rerio): Adult and embryonic expression patterns
title Growth hormone receptors in zebrafish (Danio rerio): Adult and embryonic expression patterns
spellingShingle Growth hormone receptors in zebrafish (Danio rerio): Adult and embryonic expression patterns
Di Prinzio, Cecilia Mayra
ZEBRAFISH
GROWTH HORMONE RECEPTOR
GROWTH HORMONE
DEVELOPMENT
ALTERNATIVE TRANSCRIPTS
IN SITU HYBRIDIZATION
YOLK CELL
title_short Growth hormone receptors in zebrafish (Danio rerio): Adult and embryonic expression patterns
title_full Growth hormone receptors in zebrafish (Danio rerio): Adult and embryonic expression patterns
title_fullStr Growth hormone receptors in zebrafish (Danio rerio): Adult and embryonic expression patterns
title_full_unstemmed Growth hormone receptors in zebrafish (Danio rerio): Adult and embryonic expression patterns
title_sort Growth hormone receptors in zebrafish (Danio rerio): Adult and embryonic expression patterns
dc.creator.none.fl_str_mv Di Prinzio, Cecilia Mayra
Botta, Pablo Eduardo
Barriga, Elías H.
Ríos, Eduardo A.
Reyes, Ariel E.
Arranz, Silvia Eda
author Di Prinzio, Cecilia Mayra
author_facet Di Prinzio, Cecilia Mayra
Botta, Pablo Eduardo
Barriga, Elías H.
Ríos, Eduardo A.
Reyes, Ariel E.
Arranz, Silvia Eda
author_role author
author2 Botta, Pablo Eduardo
Barriga, Elías H.
Ríos, Eduardo A.
Reyes, Ariel E.
Arranz, Silvia Eda
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv ZEBRAFISH
GROWTH HORMONE RECEPTOR
GROWTH HORMONE
DEVELOPMENT
ALTERNATIVE TRANSCRIPTS
IN SITU HYBRIDIZATION
YOLK CELL
topic ZEBRAFISH
GROWTH HORMONE RECEPTOR
GROWTH HORMONE
DEVELOPMENT
ALTERNATIVE TRANSCRIPTS
IN SITU HYBRIDIZATION
YOLK CELL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Growth hormone receptor (GHR) is a critical regulator of growth and metabolism. Although two GHRs have been characterized in many fish species, their functional characteristics, mechanisms of regulation and roles in embryonic development remain unclear. The zebrafish (Danio rerio) is an excellent model organism to study both developmental and physiological processes. In the present work, we characterized the complete cDNA sequences of zebrafish GHRs, ghra and ghrb, and their gene structures. We studied the expression of both receptors in adult tissues, and during embryonic development and larval stages by means of RT-PCR and whole-mount in situ hybridization. We determined that both transcripts are maternal ones, with specific expression patterns during development. Both GHR transcripts are mainly expressed in the notochord, myotomes, anterior structures and in the yolk cell. Interestingly, their expression became undetectable at 96 h post- Fertilization. Unlike other reports in fish, ghrs expression could not be detected in brain when adult tissues were used, and we detected ghrb but not ghra transcripts in muscle. In addition, we determined alternative transcript sequences for ghra with specific domain deletions, and alternative transcripts for ghrb that generate a premature stop codon and codify for truncated isoforms. These isoforms lack intracellular regions necessary for the activation of signal transducers and activators of transcription (STAT) family transcription factors 5.
Fil: Di Prinzio, Cecilia Mayra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Botta, Pablo Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Barriga, Elías H.. Universidad Andrés Bello; Chile
Fil: Ríos, Eduardo A.. Universidad Andrés Bello; Chile
Fil: Reyes, Ariel E.. Universidad Andrés Bello; Chile. Universidad Diego Portales; Chile
Fil: Arranz, Silvia Eda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
description Growth hormone receptor (GHR) is a critical regulator of growth and metabolism. Although two GHRs have been characterized in many fish species, their functional characteristics, mechanisms of regulation and roles in embryonic development remain unclear. The zebrafish (Danio rerio) is an excellent model organism to study both developmental and physiological processes. In the present work, we characterized the complete cDNA sequences of zebrafish GHRs, ghra and ghrb, and their gene structures. We studied the expression of both receptors in adult tissues, and during embryonic development and larval stages by means of RT-PCR and whole-mount in situ hybridization. We determined that both transcripts are maternal ones, with specific expression patterns during development. Both GHR transcripts are mainly expressed in the notochord, myotomes, anterior structures and in the yolk cell. Interestingly, their expression became undetectable at 96 h post- Fertilization. Unlike other reports in fish, ghrs expression could not be detected in brain when adult tissues were used, and we detected ghrb but not ghra transcripts in muscle. In addition, we determined alternative transcript sequences for ghra with specific domain deletions, and alternative transcripts for ghrb that generate a premature stop codon and codify for truncated isoforms. These isoforms lack intracellular regions necessary for the activation of signal transducers and activators of transcription (STAT) family transcription factors 5.
publishDate 2010
dc.date.none.fl_str_mv 2010-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/284333
Di Prinzio, Cecilia Mayra; Botta, Pablo Eduardo; Barriga, Elías H.; Ríos, Eduardo A.; Reyes, Ariel E.; et al.; Growth hormone receptors in zebrafish (Danio rerio): Adult and embryonic expression patterns; Elsevier Science; Gene Expression Patterns; 10; 4-5; 6-2010; 214-225
1567-133X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/284333
identifier_str_mv Di Prinzio, Cecilia Mayra; Botta, Pablo Eduardo; Barriga, Elías H.; Ríos, Eduardo A.; Reyes, Ariel E.; et al.; Growth hormone receptors in zebrafish (Danio rerio): Adult and embryonic expression patterns; Elsevier Science; Gene Expression Patterns; 10; 4-5; 6-2010; 214-225
1567-133X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1567133X10000268
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.gep.2010.03.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.265058

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