Galactofuranosidases: From the Initial Activity Detection to the First Crystalline Structure

Autores
Toro Melgarejo, Linda America; Marino, María Carla
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Elucidating the biosynthesis and metabolism of galactofuranosyl-containing glycoconjugates is crucial as this sugar is present in infectious microorganisms but not synthesized by mammals. This makes it a key target for understanding infection mechanisms and for the development of diagnostic methods and chemotherapeutic agents. The biosynthesis and metabolism of β-d-Galf-containing molecules involve several processing enzymes, such as UDP-Galp mutase (UGM), galactofuranosyl transferases, and galactofuranosidases. Structural elucidation of the latter has been delayed by several factors, although significant recent advances have been made. This review summarizes the discovery and functional characterization of the first galactofuranosidases using classical methods, leading up to the latest advancements, including the crystal structure of a bacterial galactofuranosidase, which will undoubtedly pave the way for the development of new tools for the control of related diseases.
Fil: Toro Melgarejo, Linda America. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Marino, María Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Materia
CARBOHYDRATES
CHEMICAL SPECIFICITY
GENETICS
PEPTIDES AND PROTEINS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/291370
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Galactofuranosidases: From the Initial Activity Detection to the First Crystalline StructureToro Melgarejo, Linda AmericaMarino, María CarlaCARBOHYDRATESCHEMICAL SPECIFICITYGENETICSPEPTIDES AND PROTEINShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Elucidating the biosynthesis and metabolism of galactofuranosyl-containing glycoconjugates is crucial as this sugar is present in infectious microorganisms but not synthesized by mammals. This makes it a key target for understanding infection mechanisms and for the development of diagnostic methods and chemotherapeutic agents. The biosynthesis and metabolism of β-d-Galf-containing molecules involve several processing enzymes, such as UDP-Galp mutase (UGM), galactofuranosyl transferases, and galactofuranosidases. Structural elucidation of the latter has been delayed by several factors, although significant recent advances have been made. This review summarizes the discovery and functional characterization of the first galactofuranosidases using classical methods, leading up to the latest advancements, including the crystal structure of a bacterial galactofuranosidase, which will undoubtedly pave the way for the development of new tools for the control of related diseases.Fil: Toro Melgarejo, Linda America. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Marino, María Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; ArgentinaAmerican Chemical Society2025-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/291370Toro Melgarejo, Linda America; Marino, María Carla; Galactofuranosidases: From the Initial Activity Detection to the First Crystalline Structure; American Chemical Society; ACS Omega; 10; 28; 7-2025; 29969-299792470-1343CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acsomega.5c04674info:eu-repo/semantics/altIdentifier/doi/10.1021/acsomega.5c04674info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:49:25Zoai:ri.conicet.gov.ar:11336/291370instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:49:25.946CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Galactofuranosidases: From the Initial Activity Detection to the First Crystalline Structure
title Galactofuranosidases: From the Initial Activity Detection to the First Crystalline Structure
spellingShingle Galactofuranosidases: From the Initial Activity Detection to the First Crystalline Structure
Toro Melgarejo, Linda America
CARBOHYDRATES
CHEMICAL SPECIFICITY
GENETICS
PEPTIDES AND PROTEINS
title_short Galactofuranosidases: From the Initial Activity Detection to the First Crystalline Structure
title_full Galactofuranosidases: From the Initial Activity Detection to the First Crystalline Structure
title_fullStr Galactofuranosidases: From the Initial Activity Detection to the First Crystalline Structure
title_full_unstemmed Galactofuranosidases: From the Initial Activity Detection to the First Crystalline Structure
title_sort Galactofuranosidases: From the Initial Activity Detection to the First Crystalline Structure
dc.creator.none.fl_str_mv Toro Melgarejo, Linda America
Marino, María Carla
author Toro Melgarejo, Linda America
author_facet Toro Melgarejo, Linda America
Marino, María Carla
author_role author
author2 Marino, María Carla
author2_role author
dc.subject.none.fl_str_mv CARBOHYDRATES
CHEMICAL SPECIFICITY
GENETICS
PEPTIDES AND PROTEINS
topic CARBOHYDRATES
CHEMICAL SPECIFICITY
GENETICS
PEPTIDES AND PROTEINS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Elucidating the biosynthesis and metabolism of galactofuranosyl-containing glycoconjugates is crucial as this sugar is present in infectious microorganisms but not synthesized by mammals. This makes it a key target for understanding infection mechanisms and for the development of diagnostic methods and chemotherapeutic agents. The biosynthesis and metabolism of β-d-Galf-containing molecules involve several processing enzymes, such as UDP-Galp mutase (UGM), galactofuranosyl transferases, and galactofuranosidases. Structural elucidation of the latter has been delayed by several factors, although significant recent advances have been made. This review summarizes the discovery and functional characterization of the first galactofuranosidases using classical methods, leading up to the latest advancements, including the crystal structure of a bacterial galactofuranosidase, which will undoubtedly pave the way for the development of new tools for the control of related diseases.
Fil: Toro Melgarejo, Linda America. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Marino, María Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
description Elucidating the biosynthesis and metabolism of galactofuranosyl-containing glycoconjugates is crucial as this sugar is present in infectious microorganisms but not synthesized by mammals. This makes it a key target for understanding infection mechanisms and for the development of diagnostic methods and chemotherapeutic agents. The biosynthesis and metabolism of β-d-Galf-containing molecules involve several processing enzymes, such as UDP-Galp mutase (UGM), galactofuranosyl transferases, and galactofuranosidases. Structural elucidation of the latter has been delayed by several factors, although significant recent advances have been made. This review summarizes the discovery and functional characterization of the first galactofuranosidases using classical methods, leading up to the latest advancements, including the crystal structure of a bacterial galactofuranosidase, which will undoubtedly pave the way for the development of new tools for the control of related diseases.
publishDate 2025
dc.date.none.fl_str_mv 2025-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/291370
Toro Melgarejo, Linda America; Marino, María Carla; Galactofuranosidases: From the Initial Activity Detection to the First Crystalline Structure; American Chemical Society; ACS Omega; 10; 28; 7-2025; 29969-29979
2470-1343
CONICET Digital
CONICET
url http://hdl.handle.net/11336/291370
identifier_str_mv Toro Melgarejo, Linda America; Marino, María Carla; Galactofuranosidases: From the Initial Activity Detection to the First Crystalline Structure; American Chemical Society; ACS Omega; 10; 28; 7-2025; 29969-29979
2470-1343
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acsomega.5c04674
info:eu-repo/semantics/altIdentifier/doi/10.1021/acsomega.5c04674
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.265058

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