Comparative maxicircle analysis in Trypanosoma species from the LSRM clade highlights patterns in an underexplored lineage

Autores
Rusman, Fanny; Aramayo, Valeria Abigail; Floridia Yapur, Noelia Aldana del Rosario; Díaz, Anahí Guadalupe; Ponce, Tatiana Macarena Evelin; Hodi Diaz, Ana Soledad; Aguirre, Juan José; Greif, Gonzalo; Berná, Luisa; Robello, Carlos; Diosque, Patricio; Tomasini, Nicolás
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Trypanosoma lainsoni, Trypanosoma platydactyli, and Trypanosoma scelopori are kinetoplastid flagellates belonging to the LSRM clade of the genus Trypanosoma. These parasites have a unique mitochondrial DNA structure, the kinetoplast, comprising catenated maxicircles and minicircles. However, genetic information on the kinetoplasts of these species remains unknown. In this study, we assembled and analyzed maxicircles from different isolates of T. lainsoni, T. platydactyli, and T. scelopori to address the current gap in genetic information about the LSRM clade and explore their phylogenetic relationships within the Trypanosoma genus. The maxicircle of T. lainsoni isolate Le29, including the coding and divergent regions, was de novo assembled combining Illumina and Oxford Nanopore Technologies, revealing a length of 49,306 bp. Additional isolates of T. lainsoni (Ca37 and Ca47), as well as T. platydactyli and T. scelopori, were sequenced with Illumina, yielding complete coding regions and partial divergent regions for all. As in other trypanosomes, coding regions exhibited conserved synteny in gene order and RNA editing patterns. We found that mRNA editing by U-insertion was higher in T. lainsoni than in T. cruzi, suggesting a partial loss of editing in the latter. Phylogenetic analyses based on the coding region positioned the LSRM clade alongside the Aquatic clade as one of the most ancestral groups within non-salivarian trypanosomes and supported the ancestral position of the Trypanosoma brucei clade, contrasting with previous reports. Finally, analysis of the maxicircle divergent region suggests a gradual transition from long to short repeat structures in non-salivarian trypanosomes. This study provides the first characterization of the T. lainsoni maxicircle and related LSRM clade species. These findings provide new insights into the ancestral relationships within the group, the evolution of the divergent region of the maxicircles and propose RNA editing has been evolving within the genus.
Fil: Rusman, Fanny. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Aramayo, Valeria Abigail. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Floridia Yapur, Noelia Aldana del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Díaz, Anahí Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Ponce, Tatiana Macarena Evelin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Hodi Diaz, Ana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Aguirre, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Greif, Gonzalo. Instituto Pasteur de Montevideo; Uruguay
Fil: Berná, Luisa. Instituto Pasteur de Montevideo; Uruguay
Fil: Robello, Carlos. Instituto Pasteur de Montevideo; Uruguay
Fil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Tomasini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Materia
Trypanosoma lainsoni
maxicírculos
Oxford Nanopore Technologies
Análisis filogenético
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/291548
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Comparative maxicircle analysis in Trypanosoma species from the LSRM clade highlights patterns in an underexplored lineageRusman, FannyAramayo, Valeria AbigailFloridia Yapur, Noelia Aldana del RosarioDíaz, Anahí GuadalupePonce, Tatiana Macarena EvelinHodi Diaz, Ana SoledadAguirre, Juan JoséGreif, GonzaloBerná, LuisaRobello, CarlosDiosque, PatricioTomasini, NicolásTrypanosoma lainsonimaxicírculosOxford Nanopore TechnologiesAnálisis filogenéticohttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Trypanosoma lainsoni, Trypanosoma platydactyli, and Trypanosoma scelopori are kinetoplastid flagellates belonging to the LSRM clade of the genus Trypanosoma. These parasites have a unique mitochondrial DNA structure, the kinetoplast, comprising catenated maxicircles and minicircles. However, genetic information on the kinetoplasts of these species remains unknown. In this study, we assembled and analyzed maxicircles from different isolates of T. lainsoni, T. platydactyli, and T. scelopori to address the current gap in genetic information about the LSRM clade and explore their phylogenetic relationships within the Trypanosoma genus. The maxicircle of T. lainsoni isolate Le29, including the coding and divergent regions, was de novo assembled combining Illumina and Oxford Nanopore Technologies, revealing a length of 49,306 bp. Additional isolates of T. lainsoni (Ca37 and Ca47), as well as T. platydactyli and T. scelopori, were sequenced with Illumina, yielding complete coding regions and partial divergent regions for all. As in other trypanosomes, coding regions exhibited conserved synteny in gene order and RNA editing patterns. We found that mRNA editing by U-insertion was higher in T. lainsoni than in T. cruzi, suggesting a partial loss of editing in the latter. Phylogenetic analyses based on the coding region positioned the LSRM clade alongside the Aquatic clade as one of the most ancestral groups within non-salivarian trypanosomes and supported the ancestral position of the Trypanosoma brucei clade, contrasting with previous reports. Finally, analysis of the maxicircle divergent region suggests a gradual transition from long to short repeat structures in non-salivarian trypanosomes. This study provides the first characterization of the T. lainsoni maxicircle and related LSRM clade species. These findings provide new insights into the ancestral relationships within the group, the evolution of the divergent region of the maxicircles and propose RNA editing has been evolving within the genus.Fil: Rusman, Fanny. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Aramayo, Valeria Abigail. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Floridia Yapur, Noelia Aldana del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Díaz, Anahí Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Ponce, Tatiana Macarena Evelin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Hodi Diaz, Ana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Aguirre, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Greif, Gonzalo. Instituto Pasteur de Montevideo; UruguayFil: Berná, Luisa. Instituto Pasteur de Montevideo; UruguayFil: Robello, Carlos. Instituto Pasteur de Montevideo; UruguayFil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Tomasini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaPublic Library of Science2025-09-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/291548Rusman, Fanny; Aramayo, Valeria Abigail; Floridia Yapur, Noelia Aldana del Rosario; Díaz, Anahí Guadalupe; Ponce, Tatiana Macarena Evelin; et al.; Comparative maxicircle analysis in Trypanosoma species from the LSRM clade highlights patterns in an underexplored lineage; Public Library of Science; Plos One; 20; 9; 4-9-2025; 1-191932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://dx.plos.org/10.1371/journal.pone.0332749info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0332749info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T15:33:32Zoai:ri.conicet.gov.ar:11336/291548instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 15:33:32.981CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Comparative maxicircle analysis in Trypanosoma species from the LSRM clade highlights patterns in an underexplored lineage
title Comparative maxicircle analysis in Trypanosoma species from the LSRM clade highlights patterns in an underexplored lineage
spellingShingle Comparative maxicircle analysis in Trypanosoma species from the LSRM clade highlights patterns in an underexplored lineage
Rusman, Fanny
Trypanosoma lainsoni
maxicírculos
Oxford Nanopore Technologies
Análisis filogenético
title_short Comparative maxicircle analysis in Trypanosoma species from the LSRM clade highlights patterns in an underexplored lineage
title_full Comparative maxicircle analysis in Trypanosoma species from the LSRM clade highlights patterns in an underexplored lineage
title_fullStr Comparative maxicircle analysis in Trypanosoma species from the LSRM clade highlights patterns in an underexplored lineage
title_full_unstemmed Comparative maxicircle analysis in Trypanosoma species from the LSRM clade highlights patterns in an underexplored lineage
title_sort Comparative maxicircle analysis in Trypanosoma species from the LSRM clade highlights patterns in an underexplored lineage
dc.creator.none.fl_str_mv Rusman, Fanny
Aramayo, Valeria Abigail
Floridia Yapur, Noelia Aldana del Rosario
Díaz, Anahí Guadalupe
Ponce, Tatiana Macarena Evelin
Hodi Diaz, Ana Soledad
Aguirre, Juan José
Greif, Gonzalo
Berná, Luisa
Robello, Carlos
Diosque, Patricio
Tomasini, Nicolás
author Rusman, Fanny
author_facet Rusman, Fanny
Aramayo, Valeria Abigail
Floridia Yapur, Noelia Aldana del Rosario
Díaz, Anahí Guadalupe
Ponce, Tatiana Macarena Evelin
Hodi Diaz, Ana Soledad
Aguirre, Juan José
Greif, Gonzalo
Berná, Luisa
Robello, Carlos
Diosque, Patricio
Tomasini, Nicolás
author_role author
author2 Aramayo, Valeria Abigail
Floridia Yapur, Noelia Aldana del Rosario
Díaz, Anahí Guadalupe
Ponce, Tatiana Macarena Evelin
Hodi Diaz, Ana Soledad
Aguirre, Juan José
Greif, Gonzalo
Berná, Luisa
Robello, Carlos
Diosque, Patricio
Tomasini, Nicolás
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Trypanosoma lainsoni
maxicírculos
Oxford Nanopore Technologies
Análisis filogenético
topic Trypanosoma lainsoni
maxicírculos
Oxford Nanopore Technologies
Análisis filogenético
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Trypanosoma lainsoni, Trypanosoma platydactyli, and Trypanosoma scelopori are kinetoplastid flagellates belonging to the LSRM clade of the genus Trypanosoma. These parasites have a unique mitochondrial DNA structure, the kinetoplast, comprising catenated maxicircles and minicircles. However, genetic information on the kinetoplasts of these species remains unknown. In this study, we assembled and analyzed maxicircles from different isolates of T. lainsoni, T. platydactyli, and T. scelopori to address the current gap in genetic information about the LSRM clade and explore their phylogenetic relationships within the Trypanosoma genus. The maxicircle of T. lainsoni isolate Le29, including the coding and divergent regions, was de novo assembled combining Illumina and Oxford Nanopore Technologies, revealing a length of 49,306 bp. Additional isolates of T. lainsoni (Ca37 and Ca47), as well as T. platydactyli and T. scelopori, were sequenced with Illumina, yielding complete coding regions and partial divergent regions for all. As in other trypanosomes, coding regions exhibited conserved synteny in gene order and RNA editing patterns. We found that mRNA editing by U-insertion was higher in T. lainsoni than in T. cruzi, suggesting a partial loss of editing in the latter. Phylogenetic analyses based on the coding region positioned the LSRM clade alongside the Aquatic clade as one of the most ancestral groups within non-salivarian trypanosomes and supported the ancestral position of the Trypanosoma brucei clade, contrasting with previous reports. Finally, analysis of the maxicircle divergent region suggests a gradual transition from long to short repeat structures in non-salivarian trypanosomes. This study provides the first characterization of the T. lainsoni maxicircle and related LSRM clade species. These findings provide new insights into the ancestral relationships within the group, the evolution of the divergent region of the maxicircles and propose RNA editing has been evolving within the genus.
Fil: Rusman, Fanny. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Aramayo, Valeria Abigail. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Floridia Yapur, Noelia Aldana del Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Díaz, Anahí Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Ponce, Tatiana Macarena Evelin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Hodi Diaz, Ana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Aguirre, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Greif, Gonzalo. Instituto Pasteur de Montevideo; Uruguay
Fil: Berná, Luisa. Instituto Pasteur de Montevideo; Uruguay
Fil: Robello, Carlos. Instituto Pasteur de Montevideo; Uruguay
Fil: Diosque, Patricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
Fil: Tomasini, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; Argentina
description Trypanosoma lainsoni, Trypanosoma platydactyli, and Trypanosoma scelopori are kinetoplastid flagellates belonging to the LSRM clade of the genus Trypanosoma. These parasites have a unique mitochondrial DNA structure, the kinetoplast, comprising catenated maxicircles and minicircles. However, genetic information on the kinetoplasts of these species remains unknown. In this study, we assembled and analyzed maxicircles from different isolates of T. lainsoni, T. platydactyli, and T. scelopori to address the current gap in genetic information about the LSRM clade and explore their phylogenetic relationships within the Trypanosoma genus. The maxicircle of T. lainsoni isolate Le29, including the coding and divergent regions, was de novo assembled combining Illumina and Oxford Nanopore Technologies, revealing a length of 49,306 bp. Additional isolates of T. lainsoni (Ca37 and Ca47), as well as T. platydactyli and T. scelopori, were sequenced with Illumina, yielding complete coding regions and partial divergent regions for all. As in other trypanosomes, coding regions exhibited conserved synteny in gene order and RNA editing patterns. We found that mRNA editing by U-insertion was higher in T. lainsoni than in T. cruzi, suggesting a partial loss of editing in the latter. Phylogenetic analyses based on the coding region positioned the LSRM clade alongside the Aquatic clade as one of the most ancestral groups within non-salivarian trypanosomes and supported the ancestral position of the Trypanosoma brucei clade, contrasting with previous reports. Finally, analysis of the maxicircle divergent region suggests a gradual transition from long to short repeat structures in non-salivarian trypanosomes. This study provides the first characterization of the T. lainsoni maxicircle and related LSRM clade species. These findings provide new insights into the ancestral relationships within the group, the evolution of the divergent region of the maxicircles and propose RNA editing has been evolving within the genus.
publishDate 2025
dc.date.none.fl_str_mv 2025-09-04
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/291548
Rusman, Fanny; Aramayo, Valeria Abigail; Floridia Yapur, Noelia Aldana del Rosario; Díaz, Anahí Guadalupe; Ponce, Tatiana Macarena Evelin; et al.; Comparative maxicircle analysis in Trypanosoma species from the LSRM clade highlights patterns in an underexplored lineage; Public Library of Science; Plos One; 20; 9; 4-9-2025; 1-19
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/291548
identifier_str_mv Rusman, Fanny; Aramayo, Valeria Abigail; Floridia Yapur, Noelia Aldana del Rosario; Díaz, Anahí Guadalupe; Ponce, Tatiana Macarena Evelin; et al.; Comparative maxicircle analysis in Trypanosoma species from the LSRM clade highlights patterns in an underexplored lineage; Public Library of Science; Plos One; 20; 9; 4-9-2025; 1-19
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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