Antibiofilm activity of lactose and κ-carrabiose chitosan derivates

Autores
Cingolani, Romina; Buzzola, Fernanda Roxana; Navarro, Diego Alberto
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The use of natural polysaccharides, particularly chitosan and its derivatives, as agents to prevent biofilm formationand attenuate virulence properties of pathogenic bacteria has received increasing attention in recentyears. In this study chitosan and some selected derivatives were tested for their antibacterial activity, with a Nalkylatedlactose chitosan derivative and a N-alkylated κ-carrabiose chitosan derivative showing promising resultsas inhibitors of in vitro biofilm formation in the bacterial strain Staphylococcus aureus ATCC25923. Incombination with the antibiotic ciprofloxacin, the selected lactose derivative showed a reduction in biofilmformation compared to the use of the antibiotic alone and was effective in alleviating the enhancement of biofilmformation that arrives as an unwanted effect of the antibiotic use. The novel κ-carrabiose-chitosan derivativeswere synthesized using response surface methodology (RSM) and products with different degrees of substitutionwere obtained in a controlled manner. The reaction conditions were selected based on the prior optimization byRSM of the influential variables (reagent concentrations, volume, time and temperature) in the synthesis oflactose-chitosan derivatives. The reductive amination reaction using 2-picoline borane as a reducing agent wasselected to achieve the synthesis of the chitosan derivatives, making the reaction and work-up greener and safer.
Fil: Cingolani, Romina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Subsede del Centro de Investigaciones en Hidratos de Carbono | Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono. Subsede del Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Buzzola, Fernanda Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Navarro, Diego Alberto. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Subsede del Centro de Investigaciones en Hidratos de Carbono | Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono. Subsede del Centro de Investigaciones en Hidratos de Carbono; Argentina
Materia
Chitosan
Reductive amination
Response surface methodology
Antibiofilm activity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/288557
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antibiofilm activity of lactose and κ-carrabiose chitosan derivatesCingolani, RominaBuzzola, Fernanda RoxanaNavarro, Diego AlbertoChitosanReductive aminationResponse surface methodologyAntibiofilm activityhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The use of natural polysaccharides, particularly chitosan and its derivatives, as agents to prevent biofilm formationand attenuate virulence properties of pathogenic bacteria has received increasing attention in recentyears. In this study chitosan and some selected derivatives were tested for their antibacterial activity, with a Nalkylatedlactose chitosan derivative and a N-alkylated κ-carrabiose chitosan derivative showing promising resultsas inhibitors of in vitro biofilm formation in the bacterial strain Staphylococcus aureus ATCC25923. Incombination with the antibiotic ciprofloxacin, the selected lactose derivative showed a reduction in biofilmformation compared to the use of the antibiotic alone and was effective in alleviating the enhancement of biofilmformation that arrives as an unwanted effect of the antibiotic use. The novel κ-carrabiose-chitosan derivativeswere synthesized using response surface methodology (RSM) and products with different degrees of substitutionwere obtained in a controlled manner. The reaction conditions were selected based on the prior optimization byRSM of the influential variables (reagent concentrations, volume, time and temperature) in the synthesis oflactose-chitosan derivatives. The reductive amination reaction using 2-picoline borane as a reducing agent wasselected to achieve the synthesis of the chitosan derivatives, making the reaction and work-up greener and safer.Fil: Cingolani, Romina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Subsede del Centro de Investigaciones en Hidratos de Carbono | Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono. Subsede del Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Buzzola, Fernanda Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Navarro, Diego Alberto. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Subsede del Centro de Investigaciones en Hidratos de Carbono | Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono. Subsede del Centro de Investigaciones en Hidratos de Carbono; ArgentinaElsevier2026-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/288557Cingolani, Romina; Buzzola, Fernanda Roxana; Navarro, Diego Alberto; Antibiofilm activity of lactose and κ-carrabiose chitosan derivates; Elsevier; Carbohydrate Polymer Technologies and Applications; 13; 3-2026; 1-132666-8939CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2666893925004220info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carpta.2025.101082info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:37:59Zoai:ri.conicet.gov.ar:11336/288557instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:38:00.086CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antibiofilm activity of lactose and κ-carrabiose chitosan derivates
title Antibiofilm activity of lactose and κ-carrabiose chitosan derivates
spellingShingle Antibiofilm activity of lactose and κ-carrabiose chitosan derivates
Cingolani, Romina
Chitosan
Reductive amination
Response surface methodology
Antibiofilm activity
title_short Antibiofilm activity of lactose and κ-carrabiose chitosan derivates
title_full Antibiofilm activity of lactose and κ-carrabiose chitosan derivates
title_fullStr Antibiofilm activity of lactose and κ-carrabiose chitosan derivates
title_full_unstemmed Antibiofilm activity of lactose and κ-carrabiose chitosan derivates
title_sort Antibiofilm activity of lactose and κ-carrabiose chitosan derivates
dc.creator.none.fl_str_mv Cingolani, Romina
Buzzola, Fernanda Roxana
Navarro, Diego Alberto
author Cingolani, Romina
author_facet Cingolani, Romina
Buzzola, Fernanda Roxana
Navarro, Diego Alberto
author_role author
author2 Buzzola, Fernanda Roxana
Navarro, Diego Alberto
author2_role author
author
dc.subject.none.fl_str_mv Chitosan
Reductive amination
Response surface methodology
Antibiofilm activity
topic Chitosan
Reductive amination
Response surface methodology
Antibiofilm activity
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The use of natural polysaccharides, particularly chitosan and its derivatives, as agents to prevent biofilm formationand attenuate virulence properties of pathogenic bacteria has received increasing attention in recentyears. In this study chitosan and some selected derivatives were tested for their antibacterial activity, with a Nalkylatedlactose chitosan derivative and a N-alkylated κ-carrabiose chitosan derivative showing promising resultsas inhibitors of in vitro biofilm formation in the bacterial strain Staphylococcus aureus ATCC25923. Incombination with the antibiotic ciprofloxacin, the selected lactose derivative showed a reduction in biofilmformation compared to the use of the antibiotic alone and was effective in alleviating the enhancement of biofilmformation that arrives as an unwanted effect of the antibiotic use. The novel κ-carrabiose-chitosan derivativeswere synthesized using response surface methodology (RSM) and products with different degrees of substitutionwere obtained in a controlled manner. The reaction conditions were selected based on the prior optimization byRSM of the influential variables (reagent concentrations, volume, time and temperature) in the synthesis oflactose-chitosan derivatives. The reductive amination reaction using 2-picoline borane as a reducing agent wasselected to achieve the synthesis of the chitosan derivatives, making the reaction and work-up greener and safer.
Fil: Cingolani, Romina. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Subsede del Centro de Investigaciones en Hidratos de Carbono | Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono. Subsede del Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Buzzola, Fernanda Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Navarro, Diego Alberto. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Subsede del Centro de Investigaciones en Hidratos de Carbono | Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono. Subsede del Centro de Investigaciones en Hidratos de Carbono; Argentina
description The use of natural polysaccharides, particularly chitosan and its derivatives, as agents to prevent biofilm formationand attenuate virulence properties of pathogenic bacteria has received increasing attention in recentyears. In this study chitosan and some selected derivatives were tested for their antibacterial activity, with a Nalkylatedlactose chitosan derivative and a N-alkylated κ-carrabiose chitosan derivative showing promising resultsas inhibitors of in vitro biofilm formation in the bacterial strain Staphylococcus aureus ATCC25923. Incombination with the antibiotic ciprofloxacin, the selected lactose derivative showed a reduction in biofilmformation compared to the use of the antibiotic alone and was effective in alleviating the enhancement of biofilmformation that arrives as an unwanted effect of the antibiotic use. The novel κ-carrabiose-chitosan derivativeswere synthesized using response surface methodology (RSM) and products with different degrees of substitutionwere obtained in a controlled manner. The reaction conditions were selected based on the prior optimization byRSM of the influential variables (reagent concentrations, volume, time and temperature) in the synthesis oflactose-chitosan derivatives. The reductive amination reaction using 2-picoline borane as a reducing agent wasselected to achieve the synthesis of the chitosan derivatives, making the reaction and work-up greener and safer.
publishDate 2026
dc.date.none.fl_str_mv 2026-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/288557
Cingolani, Romina; Buzzola, Fernanda Roxana; Navarro, Diego Alberto; Antibiofilm activity of lactose and κ-carrabiose chitosan derivates; Elsevier; Carbohydrate Polymer Technologies and Applications; 13; 3-2026; 1-13
2666-8939
CONICET Digital
CONICET
url http://hdl.handle.net/11336/288557
identifier_str_mv Cingolani, Romina; Buzzola, Fernanda Roxana; Navarro, Diego Alberto; Antibiofilm activity of lactose and κ-carrabiose chitosan derivates; Elsevier; Carbohydrate Polymer Technologies and Applications; 13; 3-2026; 1-13
2666-8939
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S2666893925004220
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carpta.2025.101082
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.265058

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