Monoterpenes As Modulators Of Cys-Loop Receptors: Insights Into Anthelmintic Activity In Caenorhabditis Elegans

Autores
Pulido Carrasquero, Alcibeth Yoselin; Bouzat, Cecilia Beatriz; Hernando, Guillermina Silvana
Año de publicación
2025
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Growing resistance to conventional anthelmintics, coupled with the limited development of new compounds, has intensified the search for alternative therapeutic strategies. Among these, combination therapy—incorporating commercial drugs, new agents, or natural products— emerges as a promising approach to broaden treatment efficacy. Monoterpenes from essential oils are particularly attractive due to their reported anthelmintic activity. Here, we evaluated the effects of geraniol and citronellol on Caenorhabditis elegans, both individually and in combination with commercial anthelmintics. C. elegans is a well-established model system for anthelmintic drug discovery. Combining bioactive phytochemicals with synthetic drugs has been proposed as a strategy to enhance nematode control in both human and veterinary medicine. Behavioral assays showed that both terpenes significantly inhibited pharyngeal pumping in wild-type worms. In glutamate-gated chloride channel (GluCl)-defi- cient mutants, inhibition was reduced, suggesting GluCl channels asrelevant targets. In contrast, ACR-23 (betaine-gated cation channel) and MOD-1 (serotonin-gated chloride channel) mutants did not differ from wild-type. Paralysis assays were performed by exposing syn- chronized adult’s worms to 0.600–0.750 mM geraniol or citronellol, respectively. Both compounds exhibited clear anthelmintic activity in a concentration-dependent manner. Combinations with anthelmint- ics were evaluated using the Chou–Talalay combination index. In addition, body length was measured and Cys-loop receptor mutant strains were employed to identify potential molecular targets. The results position Cys-loop receptors, particularly GluCl, as relevant molecular targets and highlight geraniol and citronellol as modula- tors of these channels, supporting their potential use in combined therapeutic strategies for helminth control in human and veterinary health
Fil: Pulido Carrasquero, Alcibeth Yoselin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Reunión Anual De Sociedades De Biociencias 2025; LXX Reunión Anual De La Sociedad Argentina De Investigación Clínica; LVII Reunión Anual De La Asociación Argentina De Farmacología Experimental
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina De Investigación Clínica
Reunión Anual De La Asociación Argentina De Farmacología Experimental
Materia
Essential Oils
Caenorhabditis elegans
AChR
anthelmintics
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/290072
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Monoterpenes As Modulators Of Cys-Loop Receptors: Insights Into Anthelmintic Activity In Caenorhabditis ElegansPulido Carrasquero, Alcibeth YoselinBouzat, Cecilia BeatrizHernando, Guillermina SilvanaEssential OilsCaenorhabditis elegansAChRanthelminticshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Growing resistance to conventional anthelmintics, coupled with the limited development of new compounds, has intensified the search for alternative therapeutic strategies. Among these, combination therapy—incorporating commercial drugs, new agents, or natural products— emerges as a promising approach to broaden treatment efficacy. Monoterpenes from essential oils are particularly attractive due to their reported anthelmintic activity. Here, we evaluated the effects of geraniol and citronellol on Caenorhabditis elegans, both individually and in combination with commercial anthelmintics. C. elegans is a well-established model system for anthelmintic drug discovery. Combining bioactive phytochemicals with synthetic drugs has been proposed as a strategy to enhance nematode control in both human and veterinary medicine. Behavioral assays showed that both terpenes significantly inhibited pharyngeal pumping in wild-type worms. In glutamate-gated chloride channel (GluCl)-defi- cient mutants, inhibition was reduced, suggesting GluCl channels asrelevant targets. In contrast, ACR-23 (betaine-gated cation channel) and MOD-1 (serotonin-gated chloride channel) mutants did not differ from wild-type. Paralysis assays were performed by exposing syn- chronized adult’s worms to 0.600–0.750 mM geraniol or citronellol, respectively. Both compounds exhibited clear anthelmintic activity in a concentration-dependent manner. Combinations with anthelmint- ics were evaluated using the Chou–Talalay combination index. In addition, body length was measured and Cys-loop receptor mutant strains were employed to identify potential molecular targets. The results position Cys-loop receptors, particularly GluCl, as relevant molecular targets and highlight geraniol and citronellol as modula- tors of these channels, supporting their potential use in combined therapeutic strategies for helminth control in human and veterinary healthFil: Pulido Carrasquero, Alcibeth Yoselin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaReunión Anual De Sociedades De Biociencias 2025; LXX Reunión Anual De La Sociedad Argentina De Investigación Clínica; LVII Reunión Anual De La Asociación Argentina De Farmacología ExperimentalCiudad Autónoma de Buenos AiresArgentinaSociedad Argentina De Investigación ClínicaReunión Anual De La Asociación Argentina De Farmacología ExperimentalFundación Revista MedicinaCotignola, Javier HernanSimonovich, Ventura2025info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/290072Monoterpenes As Modulators Of Cys-Loop Receptors: Insights Into Anthelmintic Activity In Caenorhabditis Elegans; Reunión Anual De Sociedades De Biociencias 2025; LXX Reunión Anual De La Sociedad Argentina De Investigación Clínica; LVII Reunión Anual De La Asociación Argentina De Farmacología Experimental; Ciudad Autónoma de Buenos Aires; Argentina; 2025; 68-691669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol85-25/s5/1s5.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:47:15Zoai:ri.conicet.gov.ar:11336/290072instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:47:15.525CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Monoterpenes As Modulators Of Cys-Loop Receptors: Insights Into Anthelmintic Activity In Caenorhabditis Elegans
title Monoterpenes As Modulators Of Cys-Loop Receptors: Insights Into Anthelmintic Activity In Caenorhabditis Elegans
spellingShingle Monoterpenes As Modulators Of Cys-Loop Receptors: Insights Into Anthelmintic Activity In Caenorhabditis Elegans
Pulido Carrasquero, Alcibeth Yoselin
Essential Oils
Caenorhabditis elegans
AChR
anthelmintics
title_short Monoterpenes As Modulators Of Cys-Loop Receptors: Insights Into Anthelmintic Activity In Caenorhabditis Elegans
title_full Monoterpenes As Modulators Of Cys-Loop Receptors: Insights Into Anthelmintic Activity In Caenorhabditis Elegans
title_fullStr Monoterpenes As Modulators Of Cys-Loop Receptors: Insights Into Anthelmintic Activity In Caenorhabditis Elegans
title_full_unstemmed Monoterpenes As Modulators Of Cys-Loop Receptors: Insights Into Anthelmintic Activity In Caenorhabditis Elegans
title_sort Monoterpenes As Modulators Of Cys-Loop Receptors: Insights Into Anthelmintic Activity In Caenorhabditis Elegans
dc.creator.none.fl_str_mv Pulido Carrasquero, Alcibeth Yoselin
Bouzat, Cecilia Beatriz
Hernando, Guillermina Silvana
author Pulido Carrasquero, Alcibeth Yoselin
author_facet Pulido Carrasquero, Alcibeth Yoselin
Bouzat, Cecilia Beatriz
Hernando, Guillermina Silvana
author_role author
author2 Bouzat, Cecilia Beatriz
Hernando, Guillermina Silvana
author2_role author
author
dc.contributor.none.fl_str_mv Cotignola, Javier Hernan
Simonovich, Ventura
dc.subject.none.fl_str_mv Essential Oils
Caenorhabditis elegans
AChR
anthelmintics
topic Essential Oils
Caenorhabditis elegans
AChR
anthelmintics
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Growing resistance to conventional anthelmintics, coupled with the limited development of new compounds, has intensified the search for alternative therapeutic strategies. Among these, combination therapy—incorporating commercial drugs, new agents, or natural products— emerges as a promising approach to broaden treatment efficacy. Monoterpenes from essential oils are particularly attractive due to their reported anthelmintic activity. Here, we evaluated the effects of geraniol and citronellol on Caenorhabditis elegans, both individually and in combination with commercial anthelmintics. C. elegans is a well-established model system for anthelmintic drug discovery. Combining bioactive phytochemicals with synthetic drugs has been proposed as a strategy to enhance nematode control in both human and veterinary medicine. Behavioral assays showed that both terpenes significantly inhibited pharyngeal pumping in wild-type worms. In glutamate-gated chloride channel (GluCl)-defi- cient mutants, inhibition was reduced, suggesting GluCl channels asrelevant targets. In contrast, ACR-23 (betaine-gated cation channel) and MOD-1 (serotonin-gated chloride channel) mutants did not differ from wild-type. Paralysis assays were performed by exposing syn- chronized adult’s worms to 0.600–0.750 mM geraniol or citronellol, respectively. Both compounds exhibited clear anthelmintic activity in a concentration-dependent manner. Combinations with anthelmint- ics were evaluated using the Chou–Talalay combination index. In addition, body length was measured and Cys-loop receptor mutant strains were employed to identify potential molecular targets. The results position Cys-loop receptors, particularly GluCl, as relevant molecular targets and highlight geraniol and citronellol as modula- tors of these channels, supporting their potential use in combined therapeutic strategies for helminth control in human and veterinary health
Fil: Pulido Carrasquero, Alcibeth Yoselin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Bouzat, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Reunión Anual De Sociedades De Biociencias 2025; LXX Reunión Anual De La Sociedad Argentina De Investigación Clínica; LVII Reunión Anual De La Asociación Argentina De Farmacología Experimental
Ciudad Autónoma de Buenos Aires
Argentina
Sociedad Argentina De Investigación Clínica
Reunión Anual De La Asociación Argentina De Farmacología Experimental
description Growing resistance to conventional anthelmintics, coupled with the limited development of new compounds, has intensified the search for alternative therapeutic strategies. Among these, combination therapy—incorporating commercial drugs, new agents, or natural products— emerges as a promising approach to broaden treatment efficacy. Monoterpenes from essential oils are particularly attractive due to their reported anthelmintic activity. Here, we evaluated the effects of geraniol and citronellol on Caenorhabditis elegans, both individually and in combination with commercial anthelmintics. C. elegans is a well-established model system for anthelmintic drug discovery. Combining bioactive phytochemicals with synthetic drugs has been proposed as a strategy to enhance nematode control in both human and veterinary medicine. Behavioral assays showed that both terpenes significantly inhibited pharyngeal pumping in wild-type worms. In glutamate-gated chloride channel (GluCl)-defi- cient mutants, inhibition was reduced, suggesting GluCl channels asrelevant targets. In contrast, ACR-23 (betaine-gated cation channel) and MOD-1 (serotonin-gated chloride channel) mutants did not differ from wild-type. Paralysis assays were performed by exposing syn- chronized adult’s worms to 0.600–0.750 mM geraniol or citronellol, respectively. Both compounds exhibited clear anthelmintic activity in a concentration-dependent manner. Combinations with anthelmint- ics were evaluated using the Chou–Talalay combination index. In addition, body length was measured and Cys-loop receptor mutant strains were employed to identify potential molecular targets. The results position Cys-loop receptors, particularly GluCl, as relevant molecular targets and highlight geraniol and citronellol as modula- tors of these channels, supporting their potential use in combined therapeutic strategies for helminth control in human and veterinary health
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/290072
Monoterpenes As Modulators Of Cys-Loop Receptors: Insights Into Anthelmintic Activity In Caenorhabditis Elegans; Reunión Anual De Sociedades De Biociencias 2025; LXX Reunión Anual De La Sociedad Argentina De Investigación Clínica; LVII Reunión Anual De La Asociación Argentina De Farmacología Experimental; Ciudad Autónoma de Buenos Aires; Argentina; 2025; 68-69
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/290072
identifier_str_mv Monoterpenes As Modulators Of Cys-Loop Receptors: Insights Into Anthelmintic Activity In Caenorhabditis Elegans; Reunión Anual De Sociedades De Biociencias 2025; LXX Reunión Anual De La Sociedad Argentina De Investigación Clínica; LVII Reunión Anual De La Asociación Argentina De Farmacología Experimental; Ciudad Autónoma de Buenos Aires; Argentina; 2025; 68-69
1669-9106
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol85-25/s5/1s5.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Fundación Revista Medicina
publisher.none.fl_str_mv Fundación Revista Medicina
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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