Au@mSiO 2 nanocomposites with large pores for protein transport

Autores
Montero Oleas, Andrea Cristina; Roupioz, Yoann; Trens, Philippe; Kodjikian, Stéphanie; Ludueña, Silvio Juan; Pietrasanta, Lia; Bilmes, Sara A.; Cattoen, Xavier
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Gold-mesoporous silica (Au@mSiO2) core–shell nanoparticles (NPs) have shown interesting potential for the loading of molecules to be delivered by plasmonic heating. In this study, we describe the unprecedented synthesis of Au@mSiO2 NPs with large pores (lp-Au@mSiO2), aiming at encapsulating large biomolecules such as proteins. Starting from recently reported Au@mSiO2 seeds with a diameter of 45 nm, two strategies are presented to grow a mesoporous shell with large pores. The most interesting NPs (lpAu@mSiO2) were obtained with the biphasic stratification approach, yielding NPs with large conical pores (10–20 nm openings), characterized in depth by N2-sorption and electron microscopies. Atomic force microscopy (AFM) with a sharp tip was used for the first time with these mesoporous materials to probe the accessibility of the pore openings. Biphasic stratification provides NPs with good colloidal and hydrolytic stabilities in aqueous saline medium (PBS) allowing the incubation of these NPs with two model proteins: horse radish peroxidase (HRP) and red fluorescent protein (RFP). lp-Au@mSiO2 exhibit a significantly larger loading capacity with respect to NPs with similar diameter, either non-porous or with narrower pores, providing evidence that the proteins can indeed be encapsulated within the pores.
Fil: Montero Oleas, Andrea Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Roupioz, Yoann. Universite Grenoble Alpes.; Francia
Fil: Trens, Philippe. Universidad San Francisco de Quito; Ecuador
Fil: Kodjikian, Stéphanie. Universite Grenoble Alpes.; Francia
Fil: Ludueña, Silvio Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Pietrasanta, Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina
Fil: Bilmes, Sara A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Cattoen, Xavier. Universite Grenoble Alpes.; Francia
Materia
NANOCOMPOSITES
CORE-SHELL NANOPARTICLES
PROTEIN-TRANSPORT
RED FLUORESCENT PROTEIN
HORSE RADISH PEROXIDASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/295257
Acceder
id CONICETDig_b8ab49986ca893e83e74a1d808cc554c
oai_identifier_str oai:ri.conicet.gov.ar:11336/295257
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Au@mSiO 2 nanocomposites with large pores for protein transportMontero Oleas, Andrea CristinaRoupioz, YoannTrens, PhilippeKodjikian, StéphanieLudueña, Silvio JuanPietrasanta, LiaBilmes, Sara A.Cattoen, XavierNANOCOMPOSITESCORE-SHELL NANOPARTICLESPROTEIN-TRANSPORTRED FLUORESCENT PROTEINHORSE RADISH PEROXIDASEhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Gold-mesoporous silica (Au@mSiO2) core–shell nanoparticles (NPs) have shown interesting potential for the loading of molecules to be delivered by plasmonic heating. In this study, we describe the unprecedented synthesis of Au@mSiO2 NPs with large pores (lp-Au@mSiO2), aiming at encapsulating large biomolecules such as proteins. Starting from recently reported Au@mSiO2 seeds with a diameter of 45 nm, two strategies are presented to grow a mesoporous shell with large pores. The most interesting NPs (lpAu@mSiO2) were obtained with the biphasic stratification approach, yielding NPs with large conical pores (10–20 nm openings), characterized in depth by N2-sorption and electron microscopies. Atomic force microscopy (AFM) with a sharp tip was used for the first time with these mesoporous materials to probe the accessibility of the pore openings. Biphasic stratification provides NPs with good colloidal and hydrolytic stabilities in aqueous saline medium (PBS) allowing the incubation of these NPs with two model proteins: horse radish peroxidase (HRP) and red fluorescent protein (RFP). lp-Au@mSiO2 exhibit a significantly larger loading capacity with respect to NPs with similar diameter, either non-porous or with narrower pores, providing evidence that the proteins can indeed be encapsulated within the pores.Fil: Montero Oleas, Andrea Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Roupioz, Yoann. Universite Grenoble Alpes.; FranciaFil: Trens, Philippe. Universidad San Francisco de Quito; EcuadorFil: Kodjikian, Stéphanie. Universite Grenoble Alpes.; FranciaFil: Ludueña, Silvio Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Pietrasanta, Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; ArgentinaFil: Bilmes, Sara A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; ArgentinaFil: Cattoen, Xavier. Universite Grenoble Alpes.; FranciaRoyal Society of Chemistry2025-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/295257Montero Oleas, Andrea Cristina; Roupioz, Yoann; Trens, Philippe; Kodjikian, Stéphanie; Ludueña, Silvio Juan; et al.; Au@mSiO 2 nanocomposites with large pores for protein transport; Royal Society of Chemistry; Journal of Materials Chemistry B; 13; 36; 8-2025; 11342-113522050-750X2050-7518CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://xlink.rsc.org/?DOI=D5TB01290Binfo:eu-repo/semantics/altIdentifier/doi/10.1039/D5TB01290Binfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T15:38:29Zoai:ri.conicet.gov.ar:11336/295257instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 15:38:29.402CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Au@mSiO 2 nanocomposites with large pores for protein transport
title Au@mSiO 2 nanocomposites with large pores for protein transport
spellingShingle Au@mSiO 2 nanocomposites with large pores for protein transport
Montero Oleas, Andrea Cristina
NANOCOMPOSITES
CORE-SHELL NANOPARTICLES
PROTEIN-TRANSPORT
RED FLUORESCENT PROTEIN
HORSE RADISH PEROXIDASE
title_short Au@mSiO 2 nanocomposites with large pores for protein transport
title_full Au@mSiO 2 nanocomposites with large pores for protein transport
title_fullStr Au@mSiO 2 nanocomposites with large pores for protein transport
title_full_unstemmed Au@mSiO 2 nanocomposites with large pores for protein transport
title_sort Au@mSiO 2 nanocomposites with large pores for protein transport
dc.creator.none.fl_str_mv Montero Oleas, Andrea Cristina
Roupioz, Yoann
Trens, Philippe
Kodjikian, Stéphanie
Ludueña, Silvio Juan
Pietrasanta, Lia
Bilmes, Sara A.
Cattoen, Xavier
author Montero Oleas, Andrea Cristina
author_facet Montero Oleas, Andrea Cristina
Roupioz, Yoann
Trens, Philippe
Kodjikian, Stéphanie
Ludueña, Silvio Juan
Pietrasanta, Lia
Bilmes, Sara A.
Cattoen, Xavier
author_role author
author2 Roupioz, Yoann
Trens, Philippe
Kodjikian, Stéphanie
Ludueña, Silvio Juan
Pietrasanta, Lia
Bilmes, Sara A.
Cattoen, Xavier
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv NANOCOMPOSITES
CORE-SHELL NANOPARTICLES
PROTEIN-TRANSPORT
RED FLUORESCENT PROTEIN
HORSE RADISH PEROXIDASE
topic NANOCOMPOSITES
CORE-SHELL NANOPARTICLES
PROTEIN-TRANSPORT
RED FLUORESCENT PROTEIN
HORSE RADISH PEROXIDASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Gold-mesoporous silica (Au@mSiO2) core–shell nanoparticles (NPs) have shown interesting potential for the loading of molecules to be delivered by plasmonic heating. In this study, we describe the unprecedented synthesis of Au@mSiO2 NPs with large pores (lp-Au@mSiO2), aiming at encapsulating large biomolecules such as proteins. Starting from recently reported Au@mSiO2 seeds with a diameter of 45 nm, two strategies are presented to grow a mesoporous shell with large pores. The most interesting NPs (lpAu@mSiO2) were obtained with the biphasic stratification approach, yielding NPs with large conical pores (10–20 nm openings), characterized in depth by N2-sorption and electron microscopies. Atomic force microscopy (AFM) with a sharp tip was used for the first time with these mesoporous materials to probe the accessibility of the pore openings. Biphasic stratification provides NPs with good colloidal and hydrolytic stabilities in aqueous saline medium (PBS) allowing the incubation of these NPs with two model proteins: horse radish peroxidase (HRP) and red fluorescent protein (RFP). lp-Au@mSiO2 exhibit a significantly larger loading capacity with respect to NPs with similar diameter, either non-porous or with narrower pores, providing evidence that the proteins can indeed be encapsulated within the pores.
Fil: Montero Oleas, Andrea Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Roupioz, Yoann. Universite Grenoble Alpes.; Francia
Fil: Trens, Philippe. Universidad San Francisco de Quito; Ecuador
Fil: Kodjikian, Stéphanie. Universite Grenoble Alpes.; Francia
Fil: Ludueña, Silvio Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina
Fil: Pietrasanta, Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina
Fil: Bilmes, Sara A.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química, Física de los Materiales, Medioambiente y Energía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química, Física de los Materiales, Medioambiente y Energía; Argentina
Fil: Cattoen, Xavier. Universite Grenoble Alpes.; Francia
description Gold-mesoporous silica (Au@mSiO2) core–shell nanoparticles (NPs) have shown interesting potential for the loading of molecules to be delivered by plasmonic heating. In this study, we describe the unprecedented synthesis of Au@mSiO2 NPs with large pores (lp-Au@mSiO2), aiming at encapsulating large biomolecules such as proteins. Starting from recently reported Au@mSiO2 seeds with a diameter of 45 nm, two strategies are presented to grow a mesoporous shell with large pores. The most interesting NPs (lpAu@mSiO2) were obtained with the biphasic stratification approach, yielding NPs with large conical pores (10–20 nm openings), characterized in depth by N2-sorption and electron microscopies. Atomic force microscopy (AFM) with a sharp tip was used for the first time with these mesoporous materials to probe the accessibility of the pore openings. Biphasic stratification provides NPs with good colloidal and hydrolytic stabilities in aqueous saline medium (PBS) allowing the incubation of these NPs with two model proteins: horse radish peroxidase (HRP) and red fluorescent protein (RFP). lp-Au@mSiO2 exhibit a significantly larger loading capacity with respect to NPs with similar diameter, either non-porous or with narrower pores, providing evidence that the proteins can indeed be encapsulated within the pores.
publishDate 2025
dc.date.none.fl_str_mv 2025-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/295257
Montero Oleas, Andrea Cristina; Roupioz, Yoann; Trens, Philippe; Kodjikian, Stéphanie; Ludueña, Silvio Juan; et al.; Au@mSiO 2 nanocomposites with large pores for protein transport; Royal Society of Chemistry; Journal of Materials Chemistry B; 13; 36; 8-2025; 11342-11352
2050-750X
2050-7518
CONICET Digital
CONICET
url http://hdl.handle.net/11336/295257
identifier_str_mv Montero Oleas, Andrea Cristina; Roupioz, Yoann; Trens, Philippe; Kodjikian, Stéphanie; Ludueña, Silvio Juan; et al.; Au@mSiO 2 nanocomposites with large pores for protein transport; Royal Society of Chemistry; Journal of Materials Chemistry B; 13; 36; 8-2025; 11342-11352
2050-750X
2050-7518
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://xlink.rsc.org/?DOI=D5TB01290B
info:eu-repo/semantics/altIdentifier/doi/10.1039/D5TB01290B
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24418

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