A refined dose metric for nanotoxicology based on surface site reactivity for oxidative potential of engineered nanomaterials

Autores
Alcolea Rodriguez, Victor; Simeone, Felice C.; Dumit, Verónica I.; Faccani, Lara; Toledo, Victoria; Haase, Andrea; Coca-López, Nicolas; Portela, Raquel; Bañares, Miguel A.
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The increasing production of engineered nanomaterials (ENMs) raises significant concerns about human and environmental exposure, making it essential to understand the mechanisms of their interaction with biological systems to manage the associated risks. To address this, we propose categorizing ENM reactivity using in chemico methodologies. Surface analysis through methanol chemisorption and temperature-programmed surface reaction allows for the determination of reactive surface sites, providing accurate estimates of effective ENM doses in toxicity studies. Additionally, antioxidant consumption assays (dithiothreitol, cysteine, and glutathione) and reactive oxygen species (ROS) generation assays (RNO and DCFH2-DA) are employed to rank the oxidative potential of ENM surface sites in a cell-free environment. Our study confirms the classification of ZnO NM-110, ZnO NM-111, CuO, and carbon black as highly oxidant ENMs, while TiO2 NM-101 and NM-105 exhibit low oxidative potential due to their acidic surface sites. In contrast, CeO2 NM-211 and NM-212 demonstrate redox surface sites. SiO2 nanomaterials (NM-200 and NM-201) are shown to be inert, with low oxidation rates and minimal reactive surface density, despite their high surface area. Quantifying reactive surface sites offers a refined dose metric for assessing ENM toxicity, advancing safe-by-design nanomaterial development.
Fil: Alcolea Rodriguez, Victor. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; España
Fil: Simeone, Felice C.. Consiglio Nazionale delle Ricerche; Italia
Fil: Dumit, Verónica I.. German Federal Institute For Risk Assessment (b.fur Risikobwertung). Departament Of Chemical And Product Safety.; Alemania
Fil: Faccani, Lara. Consiglio Nazionale delle Ricerche; Italia
Fil: Toledo, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; Argentina
Fil: Haase, Andrea. German Federal Institute For Risk Assessment (b.fur Risikobwertung). Departament Of Chemical And Product Safety.; Alemania
Fil: Coca-López, Nicolas. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; España
Fil: Portela, Raquel. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; España
Fil: Bañares, Miguel A.. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; España
Materia
NANOMATERIALS
TOXICITY
TPSR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/284803
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/284803
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A refined dose metric for nanotoxicology based on surface site reactivity for oxidative potential of engineered nanomaterialsAlcolea Rodriguez, VictorSimeone, Felice C.Dumit, Verónica I.Faccani, LaraToledo, VictoriaHaase, AndreaCoca-López, NicolasPortela, RaquelBañares, Miguel A.NANOMATERIALSTOXICITYTPSRhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2The increasing production of engineered nanomaterials (ENMs) raises significant concerns about human and environmental exposure, making it essential to understand the mechanisms of their interaction with biological systems to manage the associated risks. To address this, we propose categorizing ENM reactivity using in chemico methodologies. Surface analysis through methanol chemisorption and temperature-programmed surface reaction allows for the determination of reactive surface sites, providing accurate estimates of effective ENM doses in toxicity studies. Additionally, antioxidant consumption assays (dithiothreitol, cysteine, and glutathione) and reactive oxygen species (ROS) generation assays (RNO and DCFH2-DA) are employed to rank the oxidative potential of ENM surface sites in a cell-free environment. Our study confirms the classification of ZnO NM-110, ZnO NM-111, CuO, and carbon black as highly oxidant ENMs, while TiO2 NM-101 and NM-105 exhibit low oxidative potential due to their acidic surface sites. In contrast, CeO2 NM-211 and NM-212 demonstrate redox surface sites. SiO2 nanomaterials (NM-200 and NM-201) are shown to be inert, with low oxidation rates and minimal reactive surface density, despite their high surface area. Quantifying reactive surface sites offers a refined dose metric for assessing ENM toxicity, advancing safe-by-design nanomaterial development.Fil: Alcolea Rodriguez, Victor. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; EspañaFil: Simeone, Felice C.. Consiglio Nazionale delle Ricerche; ItaliaFil: Dumit, Verónica I.. German Federal Institute For Risk Assessment (b.fur Risikobwertung). Departament Of Chemical And Product Safety.; AlemaniaFil: Faccani, Lara. Consiglio Nazionale delle Ricerche; ItaliaFil: Toledo, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; ArgentinaFil: Haase, Andrea. German Federal Institute For Risk Assessment (b.fur Risikobwertung). Departament Of Chemical And Product Safety.; AlemaniaFil: Coca-López, Nicolas. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; EspañaFil: Portela, Raquel. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; EspañaFil: Bañares, Miguel A.. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; EspañaRoyal Society of Chemistry2025-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/284803Alcolea Rodriguez, Victor; Simeone, Felice C.; Dumit, Verónica I.; Faccani, Lara; Toledo, Victoria; et al.; A refined dose metric for nanotoxicology based on surface site reactivity for oxidative potential of engineered nanomaterials; Royal Society of Chemistry; Nanoscale Advances; 7; 10; 2-2025; 2929-29412516-0230CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://xlink.rsc.org/?DOI=D5NA00104Hinfo:eu-repo/semantics/altIdentifier/doi/10.1039/D5NA00104Hinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:36:28Zoai:ri.conicet.gov.ar:11336/284803instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:36:28.36CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A refined dose metric for nanotoxicology based on surface site reactivity for oxidative potential of engineered nanomaterials
title A refined dose metric for nanotoxicology based on surface site reactivity for oxidative potential of engineered nanomaterials
spellingShingle A refined dose metric for nanotoxicology based on surface site reactivity for oxidative potential of engineered nanomaterials
Alcolea Rodriguez, Victor
NANOMATERIALS
TOXICITY
TPSR
title_short A refined dose metric for nanotoxicology based on surface site reactivity for oxidative potential of engineered nanomaterials
title_full A refined dose metric for nanotoxicology based on surface site reactivity for oxidative potential of engineered nanomaterials
title_fullStr A refined dose metric for nanotoxicology based on surface site reactivity for oxidative potential of engineered nanomaterials
title_full_unstemmed A refined dose metric for nanotoxicology based on surface site reactivity for oxidative potential of engineered nanomaterials
title_sort A refined dose metric for nanotoxicology based on surface site reactivity for oxidative potential of engineered nanomaterials
dc.creator.none.fl_str_mv Alcolea Rodriguez, Victor
Simeone, Felice C.
Dumit, Verónica I.
Faccani, Lara
Toledo, Victoria
Haase, Andrea
Coca-López, Nicolas
Portela, Raquel
Bañares, Miguel A.
author Alcolea Rodriguez, Victor
author_facet Alcolea Rodriguez, Victor
Simeone, Felice C.
Dumit, Verónica I.
Faccani, Lara
Toledo, Victoria
Haase, Andrea
Coca-López, Nicolas
Portela, Raquel
Bañares, Miguel A.
author_role author
author2 Simeone, Felice C.
Dumit, Verónica I.
Faccani, Lara
Toledo, Victoria
Haase, Andrea
Coca-López, Nicolas
Portela, Raquel
Bañares, Miguel A.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv NANOMATERIALS
TOXICITY
TPSR
topic NANOMATERIALS
TOXICITY
TPSR
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The increasing production of engineered nanomaterials (ENMs) raises significant concerns about human and environmental exposure, making it essential to understand the mechanisms of their interaction with biological systems to manage the associated risks. To address this, we propose categorizing ENM reactivity using in chemico methodologies. Surface analysis through methanol chemisorption and temperature-programmed surface reaction allows for the determination of reactive surface sites, providing accurate estimates of effective ENM doses in toxicity studies. Additionally, antioxidant consumption assays (dithiothreitol, cysteine, and glutathione) and reactive oxygen species (ROS) generation assays (RNO and DCFH2-DA) are employed to rank the oxidative potential of ENM surface sites in a cell-free environment. Our study confirms the classification of ZnO NM-110, ZnO NM-111, CuO, and carbon black as highly oxidant ENMs, while TiO2 NM-101 and NM-105 exhibit low oxidative potential due to their acidic surface sites. In contrast, CeO2 NM-211 and NM-212 demonstrate redox surface sites. SiO2 nanomaterials (NM-200 and NM-201) are shown to be inert, with low oxidation rates and minimal reactive surface density, despite their high surface area. Quantifying reactive surface sites offers a refined dose metric for assessing ENM toxicity, advancing safe-by-design nanomaterial development.
Fil: Alcolea Rodriguez, Victor. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; España
Fil: Simeone, Felice C.. Consiglio Nazionale delle Ricerche; Italia
Fil: Dumit, Verónica I.. German Federal Institute For Risk Assessment (b.fur Risikobwertung). Departament Of Chemical And Product Safety.; Alemania
Fil: Faccani, Lara. Consiglio Nazionale delle Ricerche; Italia
Fil: Toledo, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Ciencias Aplicadas "Dr. Jorge J. Ronco". Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Ciencias Aplicadas; Argentina
Fil: Haase, Andrea. German Federal Institute For Risk Assessment (b.fur Risikobwertung). Departament Of Chemical And Product Safety.; Alemania
Fil: Coca-López, Nicolas. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; España
Fil: Portela, Raquel. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; España
Fil: Bañares, Miguel A.. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; España
description The increasing production of engineered nanomaterials (ENMs) raises significant concerns about human and environmental exposure, making it essential to understand the mechanisms of their interaction with biological systems to manage the associated risks. To address this, we propose categorizing ENM reactivity using in chemico methodologies. Surface analysis through methanol chemisorption and temperature-programmed surface reaction allows for the determination of reactive surface sites, providing accurate estimates of effective ENM doses in toxicity studies. Additionally, antioxidant consumption assays (dithiothreitol, cysteine, and glutathione) and reactive oxygen species (ROS) generation assays (RNO and DCFH2-DA) are employed to rank the oxidative potential of ENM surface sites in a cell-free environment. Our study confirms the classification of ZnO NM-110, ZnO NM-111, CuO, and carbon black as highly oxidant ENMs, while TiO2 NM-101 and NM-105 exhibit low oxidative potential due to their acidic surface sites. In contrast, CeO2 NM-211 and NM-212 demonstrate redox surface sites. SiO2 nanomaterials (NM-200 and NM-201) are shown to be inert, with low oxidation rates and minimal reactive surface density, despite their high surface area. Quantifying reactive surface sites offers a refined dose metric for assessing ENM toxicity, advancing safe-by-design nanomaterial development.
publishDate 2025
dc.date.none.fl_str_mv 2025-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/284803
Alcolea Rodriguez, Victor; Simeone, Felice C.; Dumit, Verónica I.; Faccani, Lara; Toledo, Victoria; et al.; A refined dose metric for nanotoxicology based on surface site reactivity for oxidative potential of engineered nanomaterials; Royal Society of Chemistry; Nanoscale Advances; 7; 10; 2-2025; 2929-2941
2516-0230
CONICET Digital
CONICET
url http://hdl.handle.net/11336/284803
identifier_str_mv Alcolea Rodriguez, Victor; Simeone, Felice C.; Dumit, Verónica I.; Faccani, Lara; Toledo, Victoria; et al.; A refined dose metric for nanotoxicology based on surface site reactivity for oxidative potential of engineered nanomaterials; Royal Society of Chemistry; Nanoscale Advances; 7; 10; 2-2025; 2929-2941
2516-0230
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://xlink.rsc.org/?DOI=D5NA00104H
info:eu-repo/semantics/altIdentifier/doi/10.1039/D5NA00104H
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24418

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