Holistic fractionation of spent yerba mate via a mild, two-stage flow-through ethanosolv process

Autores
Bertran-Llorens, Salvador; Zhou, Wen; Jiménez Llanos, Jorge; Euverink, Gert Jan W.; Rencoret, Jorge; Deuss, Peter J.; Palazzolo, Martín Alejandro
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Biorefineries capitalize the potential of lignocellulosic biomass to substitute fossil carbon for the chemical industry. In this study, the polyphenol-rich (27.4 wt%) lignocellulosic waste derived from the consumption of yerba mate was used as feedstock for fractionation towards valuable chemical products. HSQC NMR characterization of the polyphenol-rich fraction revealed that even after industrial processing and consumption, the lignin polymer of yerba mate still conserves a high β-O-4 content of 51 linkages per 100 C9 units and a S/G ratio of 1.4. The removal of extractives with ethanol and subsequent mild acid-catalyzed ethanosolv lignin extraction were performed on the spent yerba mate in a single flow-through reactor. This process yielded 19.3 wt% of extractives and 68% of the initial polyphenol fraction. The recovered phenolic-rich fraction showed high preservation of the lignin aryl ether structure, evidenced by the retention of 51 β-O-4 linkages per 100 C9 units. The extracted aqueous carbohydrate fraction containing a mixture of monomeric ethyl glycoside structures was quantified with a dedicated method achieving 94% mass-balance closure. Finally, the saccharified glucan-rich residue from the ethanosolv process was used to showcase the production of polyhydroxybutyrate, achieving a yield of 37 mg PHB/g monosaccharides by the thermophilic bacterium Tepidimonas taiwanensis LMG 22826.
Fil: Bertran-Llorens, Salvador. University of Groningen; Países Bajos
Fil: Zhou, Wen. University of Groningen; Países Bajos
Fil: Jiménez Llanos, Jorge. University of Groningen; Países Bajos
Fil: Euverink, Gert Jan W.. University of Groningen; Países Bajos
Fil: Rencoret, Jorge. Consejo Superior de Investigaciones Cientificas. Instituto de Recursos Naturales y Agrobiología de Sevilla.; España
Fil: Deuss, Peter J.. University of Groningen; Países Bajos
Fil: Palazzolo, Martín Alejandro. University of Groningen; Países Bajos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Materia
Yerba mate
Lignin
Biorefineries
Bioplastics
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/289867
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/289867
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Holistic fractionation of spent yerba mate via a mild, two-stage flow-through ethanosolv processBertran-Llorens, SalvadorZhou, WenJiménez Llanos, JorgeEuverink, Gert Jan W.Rencoret, JorgeDeuss, Peter J.Palazzolo, Martín AlejandroYerba mateLigninBiorefineriesBioplasticshttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2https://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Biorefineries capitalize the potential of lignocellulosic biomass to substitute fossil carbon for the chemical industry. In this study, the polyphenol-rich (27.4 wt%) lignocellulosic waste derived from the consumption of yerba mate was used as feedstock for fractionation towards valuable chemical products. HSQC NMR characterization of the polyphenol-rich fraction revealed that even after industrial processing and consumption, the lignin polymer of yerba mate still conserves a high β-O-4 content of 51 linkages per 100 C9 units and a S/G ratio of 1.4. The removal of extractives with ethanol and subsequent mild acid-catalyzed ethanosolv lignin extraction were performed on the spent yerba mate in a single flow-through reactor. This process yielded 19.3 wt% of extractives and 68% of the initial polyphenol fraction. The recovered phenolic-rich fraction showed high preservation of the lignin aryl ether structure, evidenced by the retention of 51 β-O-4 linkages per 100 C9 units. The extracted aqueous carbohydrate fraction containing a mixture of monomeric ethyl glycoside structures was quantified with a dedicated method achieving 94% mass-balance closure. Finally, the saccharified glucan-rich residue from the ethanosolv process was used to showcase the production of polyhydroxybutyrate, achieving a yield of 37 mg PHB/g monosaccharides by the thermophilic bacterium Tepidimonas taiwanensis LMG 22826.Fil: Bertran-Llorens, Salvador. University of Groningen; Países BajosFil: Zhou, Wen. University of Groningen; Países BajosFil: Jiménez Llanos, Jorge. University of Groningen; Países BajosFil: Euverink, Gert Jan W.. University of Groningen; Países BajosFil: Rencoret, Jorge. Consejo Superior de Investigaciones Cientificas. Instituto de Recursos Naturales y Agrobiología de Sevilla.; EspañaFil: Deuss, Peter J.. University of Groningen; Países BajosFil: Palazzolo, Martín Alejandro. University of Groningen; Países Bajos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; ArgentinaElsevier Science2026-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/289867Bertran-Llorens, Salvador; Zhou, Wen; Jiménez Llanos, Jorge; Euverink, Gert Jan W.; Rencoret, Jorge; et al.; Holistic fractionation of spent yerba mate via a mild, two-stage flow-through ethanosolv process; Elsevier Science; Industrial Crops and Products; 245; 5-2026; 1-120926-6690CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0926669026006722info:eu-repo/semantics/altIdentifier/doi/10.1016/j.indcrop.2026.123285info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T15:26:37Zoai:ri.conicet.gov.ar:11336/289867instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 15:26:37.399CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Holistic fractionation of spent yerba mate via a mild, two-stage flow-through ethanosolv process
title Holistic fractionation of spent yerba mate via a mild, two-stage flow-through ethanosolv process
spellingShingle Holistic fractionation of spent yerba mate via a mild, two-stage flow-through ethanosolv process
Bertran-Llorens, Salvador
Yerba mate
Lignin
Biorefineries
Bioplastics
title_short Holistic fractionation of spent yerba mate via a mild, two-stage flow-through ethanosolv process
title_full Holistic fractionation of spent yerba mate via a mild, two-stage flow-through ethanosolv process
title_fullStr Holistic fractionation of spent yerba mate via a mild, two-stage flow-through ethanosolv process
title_full_unstemmed Holistic fractionation of spent yerba mate via a mild, two-stage flow-through ethanosolv process
title_sort Holistic fractionation of spent yerba mate via a mild, two-stage flow-through ethanosolv process
dc.creator.none.fl_str_mv Bertran-Llorens, Salvador
Zhou, Wen
Jiménez Llanos, Jorge
Euverink, Gert Jan W.
Rencoret, Jorge
Deuss, Peter J.
Palazzolo, Martín Alejandro
author Bertran-Llorens, Salvador
author_facet Bertran-Llorens, Salvador
Zhou, Wen
Jiménez Llanos, Jorge
Euverink, Gert Jan W.
Rencoret, Jorge
Deuss, Peter J.
Palazzolo, Martín Alejandro
author_role author
author2 Zhou, Wen
Jiménez Llanos, Jorge
Euverink, Gert Jan W.
Rencoret, Jorge
Deuss, Peter J.
Palazzolo, Martín Alejandro
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Yerba mate
Lignin
Biorefineries
Bioplastics
topic Yerba mate
Lignin
Biorefineries
Bioplastics
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Biorefineries capitalize the potential of lignocellulosic biomass to substitute fossil carbon for the chemical industry. In this study, the polyphenol-rich (27.4 wt%) lignocellulosic waste derived from the consumption of yerba mate was used as feedstock for fractionation towards valuable chemical products. HSQC NMR characterization of the polyphenol-rich fraction revealed that even after industrial processing and consumption, the lignin polymer of yerba mate still conserves a high β-O-4 content of 51 linkages per 100 C9 units and a S/G ratio of 1.4. The removal of extractives with ethanol and subsequent mild acid-catalyzed ethanosolv lignin extraction were performed on the spent yerba mate in a single flow-through reactor. This process yielded 19.3 wt% of extractives and 68% of the initial polyphenol fraction. The recovered phenolic-rich fraction showed high preservation of the lignin aryl ether structure, evidenced by the retention of 51 β-O-4 linkages per 100 C9 units. The extracted aqueous carbohydrate fraction containing a mixture of monomeric ethyl glycoside structures was quantified with a dedicated method achieving 94% mass-balance closure. Finally, the saccharified glucan-rich residue from the ethanosolv process was used to showcase the production of polyhydroxybutyrate, achieving a yield of 37 mg PHB/g monosaccharides by the thermophilic bacterium Tepidimonas taiwanensis LMG 22826.
Fil: Bertran-Llorens, Salvador. University of Groningen; Países Bajos
Fil: Zhou, Wen. University of Groningen; Países Bajos
Fil: Jiménez Llanos, Jorge. University of Groningen; Países Bajos
Fil: Euverink, Gert Jan W.. University of Groningen; Países Bajos
Fil: Rencoret, Jorge. Consejo Superior de Investigaciones Cientificas. Instituto de Recursos Naturales y Agrobiología de Sevilla.; España
Fil: Deuss, Peter J.. University of Groningen; Países Bajos
Fil: Palazzolo, Martín Alejandro. University of Groningen; Países Bajos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
description Biorefineries capitalize the potential of lignocellulosic biomass to substitute fossil carbon for the chemical industry. In this study, the polyphenol-rich (27.4 wt%) lignocellulosic waste derived from the consumption of yerba mate was used as feedstock for fractionation towards valuable chemical products. HSQC NMR characterization of the polyphenol-rich fraction revealed that even after industrial processing and consumption, the lignin polymer of yerba mate still conserves a high β-O-4 content of 51 linkages per 100 C9 units and a S/G ratio of 1.4. The removal of extractives with ethanol and subsequent mild acid-catalyzed ethanosolv lignin extraction were performed on the spent yerba mate in a single flow-through reactor. This process yielded 19.3 wt% of extractives and 68% of the initial polyphenol fraction. The recovered phenolic-rich fraction showed high preservation of the lignin aryl ether structure, evidenced by the retention of 51 β-O-4 linkages per 100 C9 units. The extracted aqueous carbohydrate fraction containing a mixture of monomeric ethyl glycoside structures was quantified with a dedicated method achieving 94% mass-balance closure. Finally, the saccharified glucan-rich residue from the ethanosolv process was used to showcase the production of polyhydroxybutyrate, achieving a yield of 37 mg PHB/g monosaccharides by the thermophilic bacterium Tepidimonas taiwanensis LMG 22826.
publishDate 2026
dc.date.none.fl_str_mv 2026-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/289867
Bertran-Llorens, Salvador; Zhou, Wen; Jiménez Llanos, Jorge; Euverink, Gert Jan W.; Rencoret, Jorge; et al.; Holistic fractionation of spent yerba mate via a mild, two-stage flow-through ethanosolv process; Elsevier Science; Industrial Crops and Products; 245; 5-2026; 1-12
0926-6690
CONICET Digital
CONICET
url http://hdl.handle.net/11336/289867
identifier_str_mv Bertran-Llorens, Salvador; Zhou, Wen; Jiménez Llanos, Jorge; Euverink, Gert Jan W.; Rencoret, Jorge; et al.; Holistic fractionation of spent yerba mate via a mild, two-stage flow-through ethanosolv process; Elsevier Science; Industrial Crops and Products; 245; 5-2026; 1-12
0926-6690
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0926669026006722
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.indcrop.2026.123285
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.265058

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