Characterisation of the cell envelope and adhesive properties of Lactobacillus johnsonii CRL1647, a probiotic from the honeybee gut

Autores
Novicov Fanciotti, Mariana; Dentice Maidana, Stefania; Elean, Mariano Daniel; Villena, Julio Cesar; Audisio, Marcela Carina
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lactobacillus johnsonii CRL1647 has been studied due to its beneficial effects on Apis mellifera L. bee colonies. In this work, we analyzed the characteristics of its cell envelope and the relationship of this bacterial structure with adhesion. The study revealed that CRL1647 cells did not harbor S-layer proteins, whereas L. acidophilus ATCC4356, used as a positive control, showed a typical S-layer protein band. L. johnsonii CRL1647 hemagglutinated with sheep erythrocytes. Interestingly, the hemagglutination abilities of L. johnsonii CRL1647 were affected by the treatments with proteinase K and sodium metaperiodate. Transmission electron microscopy (TEM) analysis confirmed that L. acidophilus ATCC4356 has S-layer and revealed that the L. johnsonii CRL1647 cell surface was full of prolongations like ‘hairs’. These ultra-structures completely disappeared after the treatment with proteinase K. The CRL1647 strain was able to efficiently adhere to intestinal epithelial cells and was phagocyted by macrophages. Both, adhesion and phagocytosis were significantly diminished when CRL1647 cells were pretreated with proteinase K. From these results, it can be inferred that the principal molecules involved in the adherence of L. johnsonii CRL1647 have a glycoprotein structure, differing them from S-layer proteins.
Fil: Novicov Fanciotti, Mariana. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina
Fil: Dentice Maidana, Stefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Elean, Mariano Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
Materia
Lactobacillus johnsonii
cell envelope
S-layer
honey bee probiotic
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/291863
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/291863
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterisation of the cell envelope and adhesive properties of Lactobacillus johnsonii CRL1647, a probiotic from the honeybee gutNovicov Fanciotti, MarianaDentice Maidana, StefaniaElean, Mariano DanielVillena, Julio CesarAudisio, Marcela CarinaLactobacillus johnsoniicell envelopeS-layerhoney bee probiotichttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Lactobacillus johnsonii CRL1647 has been studied due to its beneficial effects on Apis mellifera L. bee colonies. In this work, we analyzed the characteristics of its cell envelope and the relationship of this bacterial structure with adhesion. The study revealed that CRL1647 cells did not harbor S-layer proteins, whereas L. acidophilus ATCC4356, used as a positive control, showed a typical S-layer protein band. L. johnsonii CRL1647 hemagglutinated with sheep erythrocytes. Interestingly, the hemagglutination abilities of L. johnsonii CRL1647 were affected by the treatments with proteinase K and sodium metaperiodate. Transmission electron microscopy (TEM) analysis confirmed that L. acidophilus ATCC4356 has S-layer and revealed that the L. johnsonii CRL1647 cell surface was full of prolongations like ‘hairs’. These ultra-structures completely disappeared after the treatment with proteinase K. The CRL1647 strain was able to efficiently adhere to intestinal epithelial cells and was phagocyted by macrophages. Both, adhesion and phagocytosis were significantly diminished when CRL1647 cells were pretreated with proteinase K. From these results, it can be inferred that the principal molecules involved in the adherence of L. johnsonii CRL1647 have a glycoprotein structure, differing them from S-layer proteins.Fil: Novicov Fanciotti, Mariana. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; ArgentinaFil: Dentice Maidana, Stefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Elean, Mariano Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; ArgentinaWageningen Academic2025-07-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/291863Novicov Fanciotti, Mariana; Dentice Maidana, Stefania; Elean, Mariano Daniel; Villena, Julio Cesar; Audisio, Marcela Carina; Characterisation of the cell envelope and adhesive properties of Lactobacillus johnsonii CRL1647, a probiotic from the honeybee gut; Wageningen Academic; Beneficial Microbes; 31-7-2025; 1-111876-2891CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://brill.com/view/journals/bm/aop/article-10.1163-18762891-bja00087/article-10.1163-18762891-bja00087.xmlinfo:eu-repo/semantics/altIdentifier/doi/10.1163/18762891-bja00087info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:31:44Zoai:ri.conicet.gov.ar:11336/291863instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:31:44.568CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterisation of the cell envelope and adhesive properties of Lactobacillus johnsonii CRL1647, a probiotic from the honeybee gut
title Characterisation of the cell envelope and adhesive properties of Lactobacillus johnsonii CRL1647, a probiotic from the honeybee gut
spellingShingle Characterisation of the cell envelope and adhesive properties of Lactobacillus johnsonii CRL1647, a probiotic from the honeybee gut
Novicov Fanciotti, Mariana
Lactobacillus johnsonii
cell envelope
S-layer
honey bee probiotic
title_short Characterisation of the cell envelope and adhesive properties of Lactobacillus johnsonii CRL1647, a probiotic from the honeybee gut
title_full Characterisation of the cell envelope and adhesive properties of Lactobacillus johnsonii CRL1647, a probiotic from the honeybee gut
title_fullStr Characterisation of the cell envelope and adhesive properties of Lactobacillus johnsonii CRL1647, a probiotic from the honeybee gut
title_full_unstemmed Characterisation of the cell envelope and adhesive properties of Lactobacillus johnsonii CRL1647, a probiotic from the honeybee gut
title_sort Characterisation of the cell envelope and adhesive properties of Lactobacillus johnsonii CRL1647, a probiotic from the honeybee gut
dc.creator.none.fl_str_mv Novicov Fanciotti, Mariana
Dentice Maidana, Stefania
Elean, Mariano Daniel
Villena, Julio Cesar
Audisio, Marcela Carina
author Novicov Fanciotti, Mariana
author_facet Novicov Fanciotti, Mariana
Dentice Maidana, Stefania
Elean, Mariano Daniel
Villena, Julio Cesar
Audisio, Marcela Carina
author_role author
author2 Dentice Maidana, Stefania
Elean, Mariano Daniel
Villena, Julio Cesar
Audisio, Marcela Carina
author2_role author
author
author
author
dc.subject.none.fl_str_mv Lactobacillus johnsonii
cell envelope
S-layer
honey bee probiotic
topic Lactobacillus johnsonii
cell envelope
S-layer
honey bee probiotic
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Lactobacillus johnsonii CRL1647 has been studied due to its beneficial effects on Apis mellifera L. bee colonies. In this work, we analyzed the characteristics of its cell envelope and the relationship of this bacterial structure with adhesion. The study revealed that CRL1647 cells did not harbor S-layer proteins, whereas L. acidophilus ATCC4356, used as a positive control, showed a typical S-layer protein band. L. johnsonii CRL1647 hemagglutinated with sheep erythrocytes. Interestingly, the hemagglutination abilities of L. johnsonii CRL1647 were affected by the treatments with proteinase K and sodium metaperiodate. Transmission electron microscopy (TEM) analysis confirmed that L. acidophilus ATCC4356 has S-layer and revealed that the L. johnsonii CRL1647 cell surface was full of prolongations like ‘hairs’. These ultra-structures completely disappeared after the treatment with proteinase K. The CRL1647 strain was able to efficiently adhere to intestinal epithelial cells and was phagocyted by macrophages. Both, adhesion and phagocytosis were significantly diminished when CRL1647 cells were pretreated with proteinase K. From these results, it can be inferred that the principal molecules involved in the adherence of L. johnsonii CRL1647 have a glycoprotein structure, differing them from S-layer proteins.
Fil: Novicov Fanciotti, Mariana. Universidad Nacional de Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina
Fil: Dentice Maidana, Stefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Elean, Mariano Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Audisio, Marcela Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
description Lactobacillus johnsonii CRL1647 has been studied due to its beneficial effects on Apis mellifera L. bee colonies. In this work, we analyzed the characteristics of its cell envelope and the relationship of this bacterial structure with adhesion. The study revealed that CRL1647 cells did not harbor S-layer proteins, whereas L. acidophilus ATCC4356, used as a positive control, showed a typical S-layer protein band. L. johnsonii CRL1647 hemagglutinated with sheep erythrocytes. Interestingly, the hemagglutination abilities of L. johnsonii CRL1647 were affected by the treatments with proteinase K and sodium metaperiodate. Transmission electron microscopy (TEM) analysis confirmed that L. acidophilus ATCC4356 has S-layer and revealed that the L. johnsonii CRL1647 cell surface was full of prolongations like ‘hairs’. These ultra-structures completely disappeared after the treatment with proteinase K. The CRL1647 strain was able to efficiently adhere to intestinal epithelial cells and was phagocyted by macrophages. Both, adhesion and phagocytosis were significantly diminished when CRL1647 cells were pretreated with proteinase K. From these results, it can be inferred that the principal molecules involved in the adherence of L. johnsonii CRL1647 have a glycoprotein structure, differing them from S-layer proteins.
publishDate 2025
dc.date.none.fl_str_mv 2025-07-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/291863
Novicov Fanciotti, Mariana; Dentice Maidana, Stefania; Elean, Mariano Daniel; Villena, Julio Cesar; Audisio, Marcela Carina; Characterisation of the cell envelope and adhesive properties of Lactobacillus johnsonii CRL1647, a probiotic from the honeybee gut; Wageningen Academic; Beneficial Microbes; 31-7-2025; 1-11
1876-2891
CONICET Digital
CONICET
url http://hdl.handle.net/11336/291863
identifier_str_mv Novicov Fanciotti, Mariana; Dentice Maidana, Stefania; Elean, Mariano Daniel; Villena, Julio Cesar; Audisio, Marcela Carina; Characterisation of the cell envelope and adhesive properties of Lactobacillus johnsonii CRL1647, a probiotic from the honeybee gut; Wageningen Academic; Beneficial Microbes; 31-7-2025; 1-11
1876-2891
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://brill.com/view/journals/bm/aop/article-10.1163-18762891-bja00087/article-10.1163-18762891-bja00087.xml
info:eu-repo/semantics/altIdentifier/doi/10.1163/18762891-bja00087
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wageningen Academic
publisher.none.fl_str_mv Wageningen Academic
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.265058

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