Global diversity of soil-transmitted helminths reveals population-biased genetic variation that impacts diagnostic targets

Autores: Papaiakovou, Marina; Waeschenbach, Andrea; Ajibola, Olumide; Ajjampur, Sitara S. R.; Anderson, Roy M.; Bailey, Robin; Benjamin Chung, Jade; Cambra Pellejà, Maria; Caro, Nicolas R.; Chaima, David; Cimino, Rubén Oscar; Cools, Piet; Cossa, Anélsio; Dunn, Julia; Galagan, Sean; Gandasegui, Javier; Grau Pujol, Berta; Houlder, Emma L.; Ibikounlé, Moudachirou; Jenkins, Timothy P.; Kalua, Khumbo; Kjetland, Eyrun F.; Krolewiecki, Alejandro Javier; Levecke, Bruno; Luty, Adrian J. F.; Williams, Steven A.; Witek McManus, Stefan; Littlewood, D. Timothy J.; Cantacessi, Cinzia; Doyle, Stephen R.
Año de publicación: 2025
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Soil-transmitted helminths (STHs) are intestinal parasites that affect over a billion people worldwide. STH control relies on microscopy-based diagnostics to monitor parasite prevalence and enable post-treatment surveillance; however, molecular diagnostics are rapidly being developed due to increased sensitivity, particularly in low-STH-prevalencesettings. Thegeneticdiversityof helminths and its potential impact on molecular diagnostics remain unclear. Usinglow-coveragegenomesequencing,weassessthegeneticsofSTHswithin worm, faecal, and purified egg samples from 27 countries, identifying differences in the genetic connectivity and diversity of STH-positive samples across regions and cryptic diversity between closely related human- and pig-infective species. We define substantial copy number and sequence variants in current diagnostic target regions and validate the impact of genetic variation on qPCR diagnostics using in vitro assays. Our study provides insights into the diversity and genomic epidemiology of STHs, highlighting both the challenges and opportunities for developing molecular diagnostics needed to support STH control efforts.
Fil: Papaiakovou, Marina. Natural History Museum; Reino Unido. University of Cambridge; Reino Unido. Wellcome Sanger Institute; Reino Unido
Fil: Waeschenbach, Andrea. Natural History Museum; Reino Unido
Fil: Ajibola, Olumide. Nigerian Institute of Medical Research; Nigeria
Fil: Ajjampur, Sitara S. R.. University of Washington; Estados Unidos. Christian Medical College Vellore; India
Fil: Anderson, Roy M.. Imperial College London; Reino Unido
Fil: Bailey, Robin. The Deworm3 Project; Estados Unidos. London School of Hygiene and Tropical Medicine; Reino Unido
Fil: Benjamin Chung, Jade. University of Stanford; Estados Unidos. Chan Zuckerberg Biohub; Estados Unidos
Fil: Cambra Pellejà, Maria. Instituto de Salud Global; España
Fil: Caro, Nicolas R.. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Chaima, David. The Deworm3 Project; Estados Unidos
Fil: Cimino, Rubén Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Cools, Piet. University of Ghent; Bélgica
Fil: Cossa, Anélsio. Centro de Investigación En Salud; Mozambique
Fil: Dunn, Julia. Imperial College. London Institute Of Medical Sciences.;
Fil: Galagan, Sean. The Deworm3 Project; Estados Unidos
Fil: Gandasegui, Javier. Instituto de Salud Global; España
Fil: Grau Pujol, Berta. Instituto de Salud Global; España. Fundación Mundo Sano; Argentina
Fil: Houlder, Emma L.. Leider University Of Medical Center; Países Bajos
Fil: Ibikounlé, Moudachirou. The Deworm3 Project; Estados Unidos
Fil: Jenkins, Timothy P.. Technical University of Denmark; Dinamarca
Fil: Kalua, Khumbo. The Deworm3 Project; Estados Unidos
Fil: Kjetland, Eyrun F.. Oslo University Hospital; Noruega
Fil: Krolewiecki, Alejandro Javier. Fundación Mundo Sano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Levecke, Bruno. University of Ghent; Bélgica
Fil: Luty, Adrian J. F.. University of Washington; Estados Unidos
Fil: Williams, Steven A.. Smith College; Estados Unidos
Fil: Witek McManus, Stefan. University of Washington; Estados Unidos
Fil: Littlewood, D. Timothy J.. Cromwell Road; Reino Unido
Fil: Cantacessi, Cinzia. University of Cambridge; Reino Unido
Fil: Doyle, Stephen R.. European Molecular Biology Laboratory. European Bioinformatics Institute.; Reino Unido
Materia: Soil transmitted helminths
genetic diversity
molecular targets
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/284842

Acceder

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spelling	Global diversity of soil-transmitted helminths reveals population-biased genetic variation that impacts diagnostic targetsPapaiakovou, MarinaWaeschenbach, AndreaAjibola, OlumideAjjampur, Sitara S. R.Anderson, Roy M.Bailey, RobinBenjamin Chung, JadeCambra Pellejà, MariaCaro, Nicolas R.Chaima, DavidCimino, Rubén OscarCools, PietCossa, AnélsioDunn, JuliaGalagan, SeanGandasegui, JavierGrau Pujol, BertaHoulder, Emma L.Ibikounlé, MoudachirouJenkins, Timothy P.Kalua, KhumboKjetland, Eyrun F.Krolewiecki, Alejandro JavierLevecke, BrunoLuty, Adrian J. F.Williams, Steven A.Witek McManus, StefanLittlewood, D. Timothy J.Cantacessi, CinziaDoyle, Stephen R.Soil transmitted helminthsgenetic diversitymolecular targetshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Soil-transmitted helminths (STHs) are intestinal parasites that affect over a billion people worldwide. STH control relies on microscopy-based diagnostics to monitor parasite prevalence and enable post-treatment surveillance; however, molecular diagnostics are rapidly being developed due to increased sensitivity, particularly in low-STH-prevalencesettings. Thegeneticdiversityof helminths and its potential impact on molecular diagnostics remain unclear. Usinglow-coveragegenomesequencing,weassessthegeneticsofSTHswithin worm, faecal, and purified egg samples from 27 countries, identifying differences in the genetic connectivity and diversity of STH-positive samples across regions and cryptic diversity between closely related human- and pig-infective species. We define substantial copy number and sequence variants in current diagnostic target regions and validate the impact of genetic variation on qPCR diagnostics using in vitro assays. Our study provides insights into the diversity and genomic epidemiology of STHs, highlighting both the challenges and opportunities for developing molecular diagnostics needed to support STH control efforts.Fil: Papaiakovou, Marina. Natural History Museum; Reino Unido. University of Cambridge; Reino Unido. Wellcome Sanger Institute; Reino UnidoFil: Waeschenbach, Andrea. Natural History Museum; Reino UnidoFil: Ajibola, Olumide. Nigerian Institute of Medical Research; NigeriaFil: Ajjampur, Sitara S. R.. University of Washington; Estados Unidos. Christian Medical College Vellore; IndiaFil: Anderson, Roy M.. Imperial College London; Reino UnidoFil: Bailey, Robin. The Deworm3 Project; Estados Unidos. London School of Hygiene and Tropical Medicine; Reino UnidoFil: Benjamin Chung, Jade. University of Stanford; Estados Unidos. Chan Zuckerberg Biohub; Estados UnidosFil: Cambra Pellejà, Maria. Instituto de Salud Global; EspañaFil: Caro, Nicolas R.. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Chaima, David. The Deworm3 Project; Estados UnidosFil: Cimino, Rubén Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Cools, Piet. University of Ghent; BélgicaFil: Cossa, Anélsio. Centro de Investigación En Salud; MozambiqueFil: Dunn, Julia. Imperial College. London Institute Of Medical Sciences.;Fil: Galagan, Sean. The Deworm3 Project; Estados UnidosFil: Gandasegui, Javier. Instituto de Salud Global; EspañaFil: Grau Pujol, Berta. Instituto de Salud Global; España. Fundación Mundo Sano; ArgentinaFil: Houlder, Emma L.. Leider University Of Medical Center; Países BajosFil: Ibikounlé, Moudachirou. The Deworm3 Project; Estados UnidosFil: Jenkins, Timothy P.. Technical University of Denmark; DinamarcaFil: Kalua, Khumbo. The Deworm3 Project; Estados UnidosFil: Kjetland, Eyrun F.. Oslo University Hospital; NoruegaFil: Krolewiecki, Alejandro Javier. Fundación Mundo Sano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Levecke, Bruno. University of Ghent; BélgicaFil: Luty, Adrian J. F.. University of Washington; Estados UnidosFil: Williams, Steven A.. Smith College; Estados UnidosFil: Witek McManus, Stefan. University of Washington; Estados UnidosFil: Littlewood, D. Timothy J.. Cromwell Road; Reino UnidoFil: Cantacessi, Cinzia. University of Cambridge; Reino UnidoFil: Doyle, Stephen R.. European Molecular Biology Laboratory. European Bioinformatics Institute.; Reino UnidoSpringer2025-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/284842Papaiakovou, Marina; Waeschenbach, Andrea; Ajibola, Olumide; Ajjampur, Sitara S. R.; Anderson, Roy M.; et al.; Global diversity of soil-transmitted helminths reveals population-biased genetic variation that impacts diagnostic targets; Springer; Nature Communications; 16; 1; 7-2025; 1-132041-1723CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41467-025-61687-0info:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-025-61687-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:32:30Zoai:ri.conicet.gov.ar:11336/284842instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:32:30.434CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	Global diversity of soil-transmitted helminths reveals population-biased genetic variation that impacts diagnostic targets
title	Global diversity of soil-transmitted helminths reveals population-biased genetic variation that impacts diagnostic targets
spellingShingle	Global diversity of soil-transmitted helminths reveals population-biased genetic variation that impacts diagnostic targets Papaiakovou, Marina Soil transmitted helminths genetic diversity molecular targets
title_short	Global diversity of soil-transmitted helminths reveals population-biased genetic variation that impacts diagnostic targets
title_full	Global diversity of soil-transmitted helminths reveals population-biased genetic variation that impacts diagnostic targets
title_fullStr	Global diversity of soil-transmitted helminths reveals population-biased genetic variation that impacts diagnostic targets
title_full_unstemmed	Global diversity of soil-transmitted helminths reveals population-biased genetic variation that impacts diagnostic targets
title_sort	Global diversity of soil-transmitted helminths reveals population-biased genetic variation that impacts diagnostic targets
dc.creator.none.fl_str_mv	Papaiakovou, Marina Waeschenbach, Andrea Ajibola, Olumide Ajjampur, Sitara S. R. Anderson, Roy M. Bailey, Robin Benjamin Chung, Jade Cambra Pellejà, Maria Caro, Nicolas R. Chaima, David Cimino, Rubén Oscar Cools, Piet Cossa, Anélsio Dunn, Julia Galagan, Sean Gandasegui, Javier Grau Pujol, Berta Houlder, Emma L. Ibikounlé, Moudachirou Jenkins, Timothy P. Kalua, Khumbo Kjetland, Eyrun F. Krolewiecki, Alejandro Javier Levecke, Bruno Luty, Adrian J. F. Williams, Steven A. Witek McManus, Stefan Littlewood, D. Timothy J. Cantacessi, Cinzia Doyle, Stephen R.
author	Papaiakovou, Marina
author_facet	Papaiakovou, Marina Waeschenbach, Andrea Ajibola, Olumide Ajjampur, Sitara S. R. Anderson, Roy M. Bailey, Robin Benjamin Chung, Jade Cambra Pellejà, Maria Caro, Nicolas R. Chaima, David Cimino, Rubén Oscar Cools, Piet Cossa, Anélsio Dunn, Julia Galagan, Sean Gandasegui, Javier Grau Pujol, Berta Houlder, Emma L. Ibikounlé, Moudachirou Jenkins, Timothy P. Kalua, Khumbo Kjetland, Eyrun F. Krolewiecki, Alejandro Javier Levecke, Bruno Luty, Adrian J. F. Williams, Steven A. Witek McManus, Stefan Littlewood, D. Timothy J. Cantacessi, Cinzia Doyle, Stephen R.
author_role	author
author2	Waeschenbach, Andrea Ajibola, Olumide Ajjampur, Sitara S. R. Anderson, Roy M. Bailey, Robin Benjamin Chung, Jade Cambra Pellejà, Maria Caro, Nicolas R. Chaima, David Cimino, Rubén Oscar Cools, Piet Cossa, Anélsio Dunn, Julia Galagan, Sean Gandasegui, Javier Grau Pujol, Berta Houlder, Emma L. Ibikounlé, Moudachirou Jenkins, Timothy P. Kalua, Khumbo Kjetland, Eyrun F. Krolewiecki, Alejandro Javier Levecke, Bruno Luty, Adrian J. F. Williams, Steven A. Witek McManus, Stefan Littlewood, D. Timothy J. Cantacessi, Cinzia Doyle, Stephen R.
author2_role	author author author author author author author author author author author author author author author author author author author author author author author author author author author author author
dc.subject.none.fl_str_mv	Soil transmitted helminths genetic diversity molecular targets
topic	Soil transmitted helminths genetic diversity molecular targets
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	Soil-transmitted helminths (STHs) are intestinal parasites that affect over a billion people worldwide. STH control relies on microscopy-based diagnostics to monitor parasite prevalence and enable post-treatment surveillance; however, molecular diagnostics are rapidly being developed due to increased sensitivity, particularly in low-STH-prevalencesettings. Thegeneticdiversityof helminths and its potential impact on molecular diagnostics remain unclear. Usinglow-coveragegenomesequencing,weassessthegeneticsofSTHswithin worm, faecal, and purified egg samples from 27 countries, identifying differences in the genetic connectivity and diversity of STH-positive samples across regions and cryptic diversity between closely related human- and pig-infective species. We define substantial copy number and sequence variants in current diagnostic target regions and validate the impact of genetic variation on qPCR diagnostics using in vitro assays. Our study provides insights into the diversity and genomic epidemiology of STHs, highlighting both the challenges and opportunities for developing molecular diagnostics needed to support STH control efforts. Fil: Papaiakovou, Marina. Natural History Museum; Reino Unido. University of Cambridge; Reino Unido. Wellcome Sanger Institute; Reino Unido Fil: Waeschenbach, Andrea. Natural History Museum; Reino Unido Fil: Ajibola, Olumide. Nigerian Institute of Medical Research; Nigeria Fil: Ajjampur, Sitara S. R.. University of Washington; Estados Unidos. Christian Medical College Vellore; India Fil: Anderson, Roy M.. Imperial College London; Reino Unido Fil: Bailey, Robin. The Deworm3 Project; Estados Unidos. London School of Hygiene and Tropical Medicine; Reino Unido Fil: Benjamin Chung, Jade. University of Stanford; Estados Unidos. Chan Zuckerberg Biohub; Estados Unidos Fil: Cambra Pellejà, Maria. Instituto de Salud Global; España Fil: Caro, Nicolas R.. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina Fil: Chaima, David. The Deworm3 Project; Estados Unidos Fil: Cimino, Rubén Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina Fil: Cools, Piet. University of Ghent; Bélgica Fil: Cossa, Anélsio. Centro de Investigación En Salud; Mozambique Fil: Dunn, Julia. Imperial College. London Institute Of Medical Sciences.; Fil: Galagan, Sean. The Deworm3 Project; Estados Unidos Fil: Gandasegui, Javier. Instituto de Salud Global; España Fil: Grau Pujol, Berta. Instituto de Salud Global; España. Fundación Mundo Sano; Argentina Fil: Houlder, Emma L.. Leider University Of Medical Center; Países Bajos Fil: Ibikounlé, Moudachirou. The Deworm3 Project; Estados Unidos Fil: Jenkins, Timothy P.. Technical University of Denmark; Dinamarca Fil: Kalua, Khumbo. The Deworm3 Project; Estados Unidos Fil: Kjetland, Eyrun F.. Oslo University Hospital; Noruega Fil: Krolewiecki, Alejandro Javier. Fundación Mundo Sano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina Fil: Levecke, Bruno. University of Ghent; Bélgica Fil: Luty, Adrian J. F.. University of Washington; Estados Unidos Fil: Williams, Steven A.. Smith College; Estados Unidos Fil: Witek McManus, Stefan. University of Washington; Estados Unidos Fil: Littlewood, D. Timothy J.. Cromwell Road; Reino Unido Fil: Cantacessi, Cinzia. University of Cambridge; Reino Unido Fil: Doyle, Stephen R.. European Molecular Biology Laboratory. European Bioinformatics Institute.; Reino Unido
description	Soil-transmitted helminths (STHs) are intestinal parasites that affect over a billion people worldwide. STH control relies on microscopy-based diagnostics to monitor parasite prevalence and enable post-treatment surveillance; however, molecular diagnostics are rapidly being developed due to increased sensitivity, particularly in low-STH-prevalencesettings. Thegeneticdiversityof helminths and its potential impact on molecular diagnostics remain unclear. Usinglow-coveragegenomesequencing,weassessthegeneticsofSTHswithin worm, faecal, and purified egg samples from 27 countries, identifying differences in the genetic connectivity and diversity of STH-positive samples across regions and cryptic diversity between closely related human- and pig-infective species. We define substantial copy number and sequence variants in current diagnostic target regions and validate the impact of genetic variation on qPCR diagnostics using in vitro assays. Our study provides insights into the diversity and genomic epidemiology of STHs, highlighting both the challenges and opportunities for developing molecular diagnostics needed to support STH control efforts.
publishDate	2025
dc.date.none.fl_str_mv	2025-07
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/284842 Papaiakovou, Marina; Waeschenbach, Andrea; Ajibola, Olumide; Ajjampur, Sitara S. R.; Anderson, Roy M.; et al.; Global diversity of soil-transmitted helminths reveals population-biased genetic variation that impacts diagnostic targets; Springer; Nature Communications; 16; 1; 7-2025; 1-13 2041-1723 CONICET Digital CONICET
url	http://hdl.handle.net/11336/284842
identifier_str_mv	Papaiakovou, Marina; Waeschenbach, Andrea; Ajibola, Olumide; Ajjampur, Sitara S. R.; Anderson, Roy M.; et al.; Global diversity of soil-transmitted helminths reveals population-biased genetic variation that impacts diagnostic targets; Springer; Nature Communications; 16; 1; 7-2025; 1-13 2041-1723 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41467-025-61687-0 info:eu-repo/semantics/altIdentifier/doi/10.1038/s41467-025-61687-0
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dc.publisher.none.fl_str_mv	Springer
publisher.none.fl_str_mv	Springer
dc.source.none.fl_str_mv	reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas
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instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
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repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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Global diversity of soil-transmitted helminths reveals population-biased genetic variation that impacts diagnostic targets

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