Genomic insights of two Acinetobacter non-baumannii strains with uncommon mechanisms of resistance leading to cefiderocol resistance

Autores
Akhtar, Usman; Moheb, Samyar; Davies Sala, Carol; Gutierrez, Joshua; Pasteran, Fernando; Tuttobene, Marisel Romina; Subils, Tomás; Cheng, Jerry; Valle, Quentin; Sharma, Rajnikant; Tolmasky, Marcelo E.; Rao, Gauri; Bonomo, Robert A.; Traglia, German M.; Ramírez, María Soledad
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The emergence of antimicrobial resistance in Acinetobacter species poses a significant clinical challenge, particularly in non-baumannii species, which are often overlooked in healthcare settings. In this study, we characterized two Acinetobacter clinical isolates, AMA204 and AMA207—identified as A. junii and A. haemolyticus, respectively—which exhibit uncommon resistance mechanisms that enable survival in the presence of cefiderocol, regardless of their initial minimum inhibitory concentration values. Whole-genome sequencing and comparative genomic analyses were performed to investigate the genetic determinants associated with their resistance profiles. Antimicrobial susceptibility testing confirmed multidrug resistance, with both isolates harboring key β-lactamase genes, including blaOXA-58, and blaNDM-1 in AMA204, and blaOXA-58 and blaPER-2in AMA207. Phylogenomic analyses revealed genetic relatedness to geographically diverse isolates, suggesting possible evolutionary trends and transmission dynamics. Additionally, iron uptake systems were analysed, highlighting potential mechanisms contributing to cefiderocol resistance together with the presence of listed β-lactamase. This study underscores the clinical relevance of non-baumannii Acinetobacter species in antimicrobial resistance and emphasizes the need for continued surveillance and novel therapeutic strategies to combat these emerging threats.
Fil: Akhtar, Usman. University of California; Estados Unidos
Fil: Moheb, Samyar. University of California; Estados Unidos
Fil: Davies Sala, Carol. No especifíca;
Fil: Gutierrez, Joshua. University of California; Estados Unidos
Fil: Pasteran, Fernando. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Cheng, Jerry. University of Southern California; Estados Unidos
Fil: Valle, Quentin. University of Southern California; Estados Unidos
Fil: Sharma, Rajnikant. University of Southern California; Estados Unidos
Fil: Tolmasky, Marcelo E.. University of California; Estados Unidos
Fil: Rao, Gauri. University of Southern California; Estados Unidos
Fil: Bonomo, Robert A.. No especifíca;
Fil: Traglia, German M.. Universidad de la República; Uruguay
Fil: Ramírez, María Soledad. University of California; Estados Unidos
Materia
ACINETOBACTER
CEFIDEROCOL
NON-BAUMANNII
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/289628
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/289628
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genomic insights of two Acinetobacter non-baumannii strains with uncommon mechanisms of resistance leading to cefiderocol resistanceAkhtar, UsmanMoheb, SamyarDavies Sala, CarolGutierrez, JoshuaPasteran, FernandoTuttobene, Marisel RominaSubils, TomásCheng, JerryValle, QuentinSharma, RajnikantTolmasky, Marcelo E.Rao, GauriBonomo, Robert A.Traglia, German M.Ramírez, María SoledadACINETOBACTERCEFIDEROCOLNON-BAUMANNIIhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The emergence of antimicrobial resistance in Acinetobacter species poses a significant clinical challenge, particularly in non-baumannii species, which are often overlooked in healthcare settings. In this study, we characterized two Acinetobacter clinical isolates, AMA204 and AMA207—identified as A. junii and A. haemolyticus, respectively—which exhibit uncommon resistance mechanisms that enable survival in the presence of cefiderocol, regardless of their initial minimum inhibitory concentration values. Whole-genome sequencing and comparative genomic analyses were performed to investigate the genetic determinants associated with their resistance profiles. Antimicrobial susceptibility testing confirmed multidrug resistance, with both isolates harboring key β-lactamase genes, including blaOXA-58, and blaNDM-1 in AMA204, and blaOXA-58 and blaPER-2in AMA207. Phylogenomic analyses revealed genetic relatedness to geographically diverse isolates, suggesting possible evolutionary trends and transmission dynamics. Additionally, iron uptake systems were analysed, highlighting potential mechanisms contributing to cefiderocol resistance together with the presence of listed β-lactamase. This study underscores the clinical relevance of non-baumannii Acinetobacter species in antimicrobial resistance and emphasizes the need for continued surveillance and novel therapeutic strategies to combat these emerging threats.Fil: Akhtar, Usman. University of California; Estados UnidosFil: Moheb, Samyar. University of California; Estados UnidosFil: Davies Sala, Carol. No especifíca;Fil: Gutierrez, Joshua. University of California; Estados UnidosFil: Pasteran, Fernando. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; ArgentinaFil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Cheng, Jerry. University of Southern California; Estados UnidosFil: Valle, Quentin. University of Southern California; Estados UnidosFil: Sharma, Rajnikant. University of Southern California; Estados UnidosFil: Tolmasky, Marcelo E.. University of California; Estados UnidosFil: Rao, Gauri. University of Southern California; Estados UnidosFil: Bonomo, Robert A.. No especifíca;Fil: Traglia, German M.. Universidad de la República; UruguayFil: Ramírez, María Soledad. University of California; Estados UnidosElsevier Science2025-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/289628Akhtar, Usman; Moheb, Samyar; Davies Sala, Carol; Gutierrez, Joshua; Pasteran, Fernando; et al.; Genomic insights of two Acinetobacter non-baumannii strains with uncommon mechanisms of resistance leading to cefiderocol resistance; Elsevier Science; Infection, Genetics and Evolution; 135; 9-2025; 1-111567-1348CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S1567134825001091info:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2025.105820info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T15:30:55Zoai:ri.conicet.gov.ar:11336/289628instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 15:30:56.032CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genomic insights of two Acinetobacter non-baumannii strains with uncommon mechanisms of resistance leading to cefiderocol resistance
title Genomic insights of two Acinetobacter non-baumannii strains with uncommon mechanisms of resistance leading to cefiderocol resistance
spellingShingle Genomic insights of two Acinetobacter non-baumannii strains with uncommon mechanisms of resistance leading to cefiderocol resistance
Akhtar, Usman
ACINETOBACTER
CEFIDEROCOL
NON-BAUMANNII
title_short Genomic insights of two Acinetobacter non-baumannii strains with uncommon mechanisms of resistance leading to cefiderocol resistance
title_full Genomic insights of two Acinetobacter non-baumannii strains with uncommon mechanisms of resistance leading to cefiderocol resistance
title_fullStr Genomic insights of two Acinetobacter non-baumannii strains with uncommon mechanisms of resistance leading to cefiderocol resistance
title_full_unstemmed Genomic insights of two Acinetobacter non-baumannii strains with uncommon mechanisms of resistance leading to cefiderocol resistance
title_sort Genomic insights of two Acinetobacter non-baumannii strains with uncommon mechanisms of resistance leading to cefiderocol resistance
dc.creator.none.fl_str_mv Akhtar, Usman
Moheb, Samyar
Davies Sala, Carol
Gutierrez, Joshua
Pasteran, Fernando
Tuttobene, Marisel Romina
Subils, Tomás
Cheng, Jerry
Valle, Quentin
Sharma, Rajnikant
Tolmasky, Marcelo E.
Rao, Gauri
Bonomo, Robert A.
Traglia, German M.
Ramírez, María Soledad
author Akhtar, Usman
author_facet Akhtar, Usman
Moheb, Samyar
Davies Sala, Carol
Gutierrez, Joshua
Pasteran, Fernando
Tuttobene, Marisel Romina
Subils, Tomás
Cheng, Jerry
Valle, Quentin
Sharma, Rajnikant
Tolmasky, Marcelo E.
Rao, Gauri
Bonomo, Robert A.
Traglia, German M.
Ramírez, María Soledad
author_role author
author2 Moheb, Samyar
Davies Sala, Carol
Gutierrez, Joshua
Pasteran, Fernando
Tuttobene, Marisel Romina
Subils, Tomás
Cheng, Jerry
Valle, Quentin
Sharma, Rajnikant
Tolmasky, Marcelo E.
Rao, Gauri
Bonomo, Robert A.
Traglia, German M.
Ramírez, María Soledad
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ACINETOBACTER
CEFIDEROCOL
NON-BAUMANNII
topic ACINETOBACTER
CEFIDEROCOL
NON-BAUMANNII
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The emergence of antimicrobial resistance in Acinetobacter species poses a significant clinical challenge, particularly in non-baumannii species, which are often overlooked in healthcare settings. In this study, we characterized two Acinetobacter clinical isolates, AMA204 and AMA207—identified as A. junii and A. haemolyticus, respectively—which exhibit uncommon resistance mechanisms that enable survival in the presence of cefiderocol, regardless of their initial minimum inhibitory concentration values. Whole-genome sequencing and comparative genomic analyses were performed to investigate the genetic determinants associated with their resistance profiles. Antimicrobial susceptibility testing confirmed multidrug resistance, with both isolates harboring key β-lactamase genes, including blaOXA-58, and blaNDM-1 in AMA204, and blaOXA-58 and blaPER-2in AMA207. Phylogenomic analyses revealed genetic relatedness to geographically diverse isolates, suggesting possible evolutionary trends and transmission dynamics. Additionally, iron uptake systems were analysed, highlighting potential mechanisms contributing to cefiderocol resistance together with the presence of listed β-lactamase. This study underscores the clinical relevance of non-baumannii Acinetobacter species in antimicrobial resistance and emphasizes the need for continued surveillance and novel therapeutic strategies to combat these emerging threats.
Fil: Akhtar, Usman. University of California; Estados Unidos
Fil: Moheb, Samyar. University of California; Estados Unidos
Fil: Davies Sala, Carol. No especifíca;
Fil: Gutierrez, Joshua. University of California; Estados Unidos
Fil: Pasteran, Fernando. Dirección Nacional de Institutos de Investigación. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas. Área de Antimicrobianos; Argentina
Fil: Tuttobene, Marisel Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Subils, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Cheng, Jerry. University of Southern California; Estados Unidos
Fil: Valle, Quentin. University of Southern California; Estados Unidos
Fil: Sharma, Rajnikant. University of Southern California; Estados Unidos
Fil: Tolmasky, Marcelo E.. University of California; Estados Unidos
Fil: Rao, Gauri. University of Southern California; Estados Unidos
Fil: Bonomo, Robert A.. No especifíca;
Fil: Traglia, German M.. Universidad de la República; Uruguay
Fil: Ramírez, María Soledad. University of California; Estados Unidos
description The emergence of antimicrobial resistance in Acinetobacter species poses a significant clinical challenge, particularly in non-baumannii species, which are often overlooked in healthcare settings. In this study, we characterized two Acinetobacter clinical isolates, AMA204 and AMA207—identified as A. junii and A. haemolyticus, respectively—which exhibit uncommon resistance mechanisms that enable survival in the presence of cefiderocol, regardless of their initial minimum inhibitory concentration values. Whole-genome sequencing and comparative genomic analyses were performed to investigate the genetic determinants associated with their resistance profiles. Antimicrobial susceptibility testing confirmed multidrug resistance, with both isolates harboring key β-lactamase genes, including blaOXA-58, and blaNDM-1 in AMA204, and blaOXA-58 and blaPER-2in AMA207. Phylogenomic analyses revealed genetic relatedness to geographically diverse isolates, suggesting possible evolutionary trends and transmission dynamics. Additionally, iron uptake systems were analysed, highlighting potential mechanisms contributing to cefiderocol resistance together with the presence of listed β-lactamase. This study underscores the clinical relevance of non-baumannii Acinetobacter species in antimicrobial resistance and emphasizes the need for continued surveillance and novel therapeutic strategies to combat these emerging threats.
publishDate 2025
dc.date.none.fl_str_mv 2025-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/289628
Akhtar, Usman; Moheb, Samyar; Davies Sala, Carol; Gutierrez, Joshua; Pasteran, Fernando; et al.; Genomic insights of two Acinetobacter non-baumannii strains with uncommon mechanisms of resistance leading to cefiderocol resistance; Elsevier Science; Infection, Genetics and Evolution; 135; 9-2025; 1-11
1567-1348
CONICET Digital
CONICET
url http://hdl.handle.net/11336/289628
identifier_str_mv Akhtar, Usman; Moheb, Samyar; Davies Sala, Carol; Gutierrez, Joshua; Pasteran, Fernando; et al.; Genomic insights of two Acinetobacter non-baumannii strains with uncommon mechanisms of resistance leading to cefiderocol resistance; Elsevier Science; Infection, Genetics and Evolution; 135; 9-2025; 1-11
1567-1348
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S1567134825001091
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2025.105820
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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