Alkaloid Profile of Fifteen Different Species of Narcissus L. (Amaryllidoideae) Collected in Spain

Autores
Rodríguez Escobar, María Lenny; Martínez Francés, Vanessa; Ríos, Segundo; Feresin, Gabriela Egly; de Souza Borges, Warley; Bastida, Jaume; Torras Claveria, Laura; Tallini, Luciana R.
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Molecular diversity is a key component of overall biodiversity, playing a vital role in evolution. It results from the adaptation of organisms to various habitats, which impacts their survival. The Amaryllidoideae subfamily is a significant group of monocotyledonous plants known for producing an exclusive and still-expanding group of molecules with diverse biological activities. Galanthamine (Gal), the most renowned metabolite from Amaryllidoideae subfamily, has been marketed for the palliative treatment of Alzheimer’s disease since 2001 due to its ability to inhibit the acetylcholinesterase enzyme. Due to the high cost and low yield of its synthesis, pharmaceutical companies extract this drug from Amaryllidoideae plants, such as Narcissus pseudonarcissus cv. Carlton in Europe and Lycoris radiata in China. The aim of this study was to describe the alkaloid profile of fifteen different species of Narcissus L. (commonly known as daffodils) collected in Spain using gas chromatography coupled with mass spectrometry. Fifty-one alkaloids were identified and quantified within these species through our private library of Amaryllidaceae alkaloids (AA) built over the last four decades, while thirty structures remained not identified in thirteen of these species. The highest concentration of these nitrogenate metabolites was quantified in N. confusus, 541 μg Gal·100 mg−1 DW, which also exhibited a notably high concentration of Gal, 301 μg Gal·100 mg−1 DW, which represents about 55% of the alkaloids identified in this species. The species N. bujei was also found to contain a significant quantity of this compound, amounting to 103.2 μg Gal·100 mg−1 DW. The plant N. assoanus harbored a total of seven unidentified compounds, indicating that this species could be a potentially important source of novel alkaloids. In conclusion, this study facilitates a direct comparison of alkaloid profiles for fifteen Narcissus plant species. This serves as a valuable tool for identifying possible new sources of galanthamine, as well as other novel medicinal alkaloids. Finally, this work presents the first alkaloid profile of the species N. minor and N. nevadensis.
Fil: Rodríguez Escobar, María Lenny. Universidad de Barcelona; España
Fil: Martínez Francés, Vanessa. Universidad de Miguel Hernández; España
Fil: Ríos, Segundo. Universidad de Alicante; España
Fil: Feresin, Gabriela Egly. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: de Souza Borges, Warley. Universidade Federal do Espírito Santo; Brasil
Fil: Bastida, Jaume. Universidad de Barcelona; España
Fil: Torras Claveria, Laura. Universidad de Barcelona; España
Fil: Tallini, Luciana R.. Universidad de Barcelona; España
Materia
AMARYLLIDOIDEAE
DAFFODILS
GAS CHROMATOGRAPHY
MOLECULAR BIODIVERSITY
NITROGENATE METABOLITES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/274521
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/274521
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Alkaloid Profile of Fifteen Different Species of Narcissus L. (Amaryllidoideae) Collected in SpainRodríguez Escobar, María LennyMartínez Francés, VanessaRíos, SegundoFeresin, Gabriela Eglyde Souza Borges, WarleyBastida, JaumeTorras Claveria, LauraTallini, Luciana R.AMARYLLIDOIDEAEDAFFODILSGAS CHROMATOGRAPHYMOLECULAR BIODIVERSITYNITROGENATE METABOLITEShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Molecular diversity is a key component of overall biodiversity, playing a vital role in evolution. It results from the adaptation of organisms to various habitats, which impacts their survival. The Amaryllidoideae subfamily is a significant group of monocotyledonous plants known for producing an exclusive and still-expanding group of molecules with diverse biological activities. Galanthamine (Gal), the most renowned metabolite from Amaryllidoideae subfamily, has been marketed for the palliative treatment of Alzheimer’s disease since 2001 due to its ability to inhibit the acetylcholinesterase enzyme. Due to the high cost and low yield of its synthesis, pharmaceutical companies extract this drug from Amaryllidoideae plants, such as Narcissus pseudonarcissus cv. Carlton in Europe and Lycoris radiata in China. The aim of this study was to describe the alkaloid profile of fifteen different species of Narcissus L. (commonly known as daffodils) collected in Spain using gas chromatography coupled with mass spectrometry. Fifty-one alkaloids were identified and quantified within these species through our private library of Amaryllidaceae alkaloids (AA) built over the last four decades, while thirty structures remained not identified in thirteen of these species. The highest concentration of these nitrogenate metabolites was quantified in N. confusus, 541 μg Gal·100 mg−1 DW, which also exhibited a notably high concentration of Gal, 301 μg Gal·100 mg−1 DW, which represents about 55% of the alkaloids identified in this species. The species N. bujei was also found to contain a significant quantity of this compound, amounting to 103.2 μg Gal·100 mg−1 DW. The plant N. assoanus harbored a total of seven unidentified compounds, indicating that this species could be a potentially important source of novel alkaloids. In conclusion, this study facilitates a direct comparison of alkaloid profiles for fifteen Narcissus plant species. This serves as a valuable tool for identifying possible new sources of galanthamine, as well as other novel medicinal alkaloids. Finally, this work presents the first alkaloid profile of the species N. minor and N. nevadensis.Fil: Rodríguez Escobar, María Lenny. Universidad de Barcelona; EspañaFil: Martínez Francés, Vanessa. Universidad de Miguel Hernández; EspañaFil: Ríos, Segundo. Universidad de Alicante; EspañaFil: Feresin, Gabriela Egly. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: de Souza Borges, Warley. Universidade Federal do Espírito Santo; BrasilFil: Bastida, Jaume. Universidad de Barcelona; EspañaFil: Torras Claveria, Laura. Universidad de Barcelona; EspañaFil: Tallini, Luciana R.. Universidad de Barcelona; EspañaMDPI2025-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/274521Rodríguez Escobar, María Lenny; Martínez Francés, Vanessa; Ríos, Segundo; Feresin, Gabriela Egly; de Souza Borges, Warley; et al.; Alkaloid Profile of Fifteen Different Species of Narcissus L. (Amaryllidoideae) Collected in Spain; MDPI; Plants; 14; 17; 9-2025; 1-122223-7747CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/14/17/2793info:eu-repo/semantics/altIdentifier/doi/10.3390/plants14172793info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:32:58Zoai:ri.conicet.gov.ar:11336/274521instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:32:58.918CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Alkaloid Profile of Fifteen Different Species of Narcissus L. (Amaryllidoideae) Collected in Spain
title Alkaloid Profile of Fifteen Different Species of Narcissus L. (Amaryllidoideae) Collected in Spain
spellingShingle Alkaloid Profile of Fifteen Different Species of Narcissus L. (Amaryllidoideae) Collected in Spain
Rodríguez Escobar, María Lenny
AMARYLLIDOIDEAE
DAFFODILS
GAS CHROMATOGRAPHY
MOLECULAR BIODIVERSITY
NITROGENATE METABOLITES
title_short Alkaloid Profile of Fifteen Different Species of Narcissus L. (Amaryllidoideae) Collected in Spain
title_full Alkaloid Profile of Fifteen Different Species of Narcissus L. (Amaryllidoideae) Collected in Spain
title_fullStr Alkaloid Profile of Fifteen Different Species of Narcissus L. (Amaryllidoideae) Collected in Spain
title_full_unstemmed Alkaloid Profile of Fifteen Different Species of Narcissus L. (Amaryllidoideae) Collected in Spain
title_sort Alkaloid Profile of Fifteen Different Species of Narcissus L. (Amaryllidoideae) Collected in Spain
dc.creator.none.fl_str_mv Rodríguez Escobar, María Lenny
Martínez Francés, Vanessa
Ríos, Segundo
Feresin, Gabriela Egly
de Souza Borges, Warley
Bastida, Jaume
Torras Claveria, Laura
Tallini, Luciana R.
author Rodríguez Escobar, María Lenny
author_facet Rodríguez Escobar, María Lenny
Martínez Francés, Vanessa
Ríos, Segundo
Feresin, Gabriela Egly
de Souza Borges, Warley
Bastida, Jaume
Torras Claveria, Laura
Tallini, Luciana R.
author_role author
author2 Martínez Francés, Vanessa
Ríos, Segundo
Feresin, Gabriela Egly
de Souza Borges, Warley
Bastida, Jaume
Torras Claveria, Laura
Tallini, Luciana R.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AMARYLLIDOIDEAE
DAFFODILS
GAS CHROMATOGRAPHY
MOLECULAR BIODIVERSITY
NITROGENATE METABOLITES
topic AMARYLLIDOIDEAE
DAFFODILS
GAS CHROMATOGRAPHY
MOLECULAR BIODIVERSITY
NITROGENATE METABOLITES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Molecular diversity is a key component of overall biodiversity, playing a vital role in evolution. It results from the adaptation of organisms to various habitats, which impacts their survival. The Amaryllidoideae subfamily is a significant group of monocotyledonous plants known for producing an exclusive and still-expanding group of molecules with diverse biological activities. Galanthamine (Gal), the most renowned metabolite from Amaryllidoideae subfamily, has been marketed for the palliative treatment of Alzheimer’s disease since 2001 due to its ability to inhibit the acetylcholinesterase enzyme. Due to the high cost and low yield of its synthesis, pharmaceutical companies extract this drug from Amaryllidoideae plants, such as Narcissus pseudonarcissus cv. Carlton in Europe and Lycoris radiata in China. The aim of this study was to describe the alkaloid profile of fifteen different species of Narcissus L. (commonly known as daffodils) collected in Spain using gas chromatography coupled with mass spectrometry. Fifty-one alkaloids were identified and quantified within these species through our private library of Amaryllidaceae alkaloids (AA) built over the last four decades, while thirty structures remained not identified in thirteen of these species. The highest concentration of these nitrogenate metabolites was quantified in N. confusus, 541 μg Gal·100 mg−1 DW, which also exhibited a notably high concentration of Gal, 301 μg Gal·100 mg−1 DW, which represents about 55% of the alkaloids identified in this species. The species N. bujei was also found to contain a significant quantity of this compound, amounting to 103.2 μg Gal·100 mg−1 DW. The plant N. assoanus harbored a total of seven unidentified compounds, indicating that this species could be a potentially important source of novel alkaloids. In conclusion, this study facilitates a direct comparison of alkaloid profiles for fifteen Narcissus plant species. This serves as a valuable tool for identifying possible new sources of galanthamine, as well as other novel medicinal alkaloids. Finally, this work presents the first alkaloid profile of the species N. minor and N. nevadensis.
Fil: Rodríguez Escobar, María Lenny. Universidad de Barcelona; España
Fil: Martínez Francés, Vanessa. Universidad de Miguel Hernández; España
Fil: Ríos, Segundo. Universidad de Alicante; España
Fil: Feresin, Gabriela Egly. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; Argentina
Fil: de Souza Borges, Warley. Universidade Federal do Espírito Santo; Brasil
Fil: Bastida, Jaume. Universidad de Barcelona; España
Fil: Torras Claveria, Laura. Universidad de Barcelona; España
Fil: Tallini, Luciana R.. Universidad de Barcelona; España
description Molecular diversity is a key component of overall biodiversity, playing a vital role in evolution. It results from the adaptation of organisms to various habitats, which impacts their survival. The Amaryllidoideae subfamily is a significant group of monocotyledonous plants known for producing an exclusive and still-expanding group of molecules with diverse biological activities. Galanthamine (Gal), the most renowned metabolite from Amaryllidoideae subfamily, has been marketed for the palliative treatment of Alzheimer’s disease since 2001 due to its ability to inhibit the acetylcholinesterase enzyme. Due to the high cost and low yield of its synthesis, pharmaceutical companies extract this drug from Amaryllidoideae plants, such as Narcissus pseudonarcissus cv. Carlton in Europe and Lycoris radiata in China. The aim of this study was to describe the alkaloid profile of fifteen different species of Narcissus L. (commonly known as daffodils) collected in Spain using gas chromatography coupled with mass spectrometry. Fifty-one alkaloids were identified and quantified within these species through our private library of Amaryllidaceae alkaloids (AA) built over the last four decades, while thirty structures remained not identified in thirteen of these species. The highest concentration of these nitrogenate metabolites was quantified in N. confusus, 541 μg Gal·100 mg−1 DW, which also exhibited a notably high concentration of Gal, 301 μg Gal·100 mg−1 DW, which represents about 55% of the alkaloids identified in this species. The species N. bujei was also found to contain a significant quantity of this compound, amounting to 103.2 μg Gal·100 mg−1 DW. The plant N. assoanus harbored a total of seven unidentified compounds, indicating that this species could be a potentially important source of novel alkaloids. In conclusion, this study facilitates a direct comparison of alkaloid profiles for fifteen Narcissus plant species. This serves as a valuable tool for identifying possible new sources of galanthamine, as well as other novel medicinal alkaloids. Finally, this work presents the first alkaloid profile of the species N. minor and N. nevadensis.
publishDate 2025
dc.date.none.fl_str_mv 2025-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/274521
Rodríguez Escobar, María Lenny; Martínez Francés, Vanessa; Ríos, Segundo; Feresin, Gabriela Egly; de Souza Borges, Warley; et al.; Alkaloid Profile of Fifteen Different Species of Narcissus L. (Amaryllidoideae) Collected in Spain; MDPI; Plants; 14; 17; 9-2025; 1-12
2223-7747
CONICET Digital
CONICET
url http://hdl.handle.net/11336/274521
identifier_str_mv Rodríguez Escobar, María Lenny; Martínez Francés, Vanessa; Ríos, Segundo; Feresin, Gabriela Egly; de Souza Borges, Warley; et al.; Alkaloid Profile of Fifteen Different Species of Narcissus L. (Amaryllidoideae) Collected in Spain; MDPI; Plants; 14; 17; 9-2025; 1-12
2223-7747
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/14/17/2793
info:eu-repo/semantics/altIdentifier/doi/10.3390/plants14172793
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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