In vitro and in vivo evidence of the antineoplastic activity of quercetin against Kaposi’s sarcoma

Autores
Principe, Gabriel; Tiburzi, Silvina Mabel; García, Betina Noemi; Gumilar, Fernanda Andrea; González Pardo, María Verónica
Año de publicación
2023
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Quercetin (QUE) is a natural flavonoid known to exhibit anticancer properties. Kaposi’s sarcoma is a viral cancer caused by the human herpesvirus-8, which expresses a constitutively activated G protein-coupled receptor (vGPCR) during its lytic phase, able to induce oncogenic modifications that lead to tumor development. The goal of this work was to explore the antineoplastic effect of QUE in an in vitro and in vivo model of Kaposi’s sarcoma. Firstly, the antiproliferative effect of QUE was determined by crystal violet technique at different concentrations of QUE (1 - 100 µM) or vehicle (DMSO) for 48 h in endothelial cells stably expressing the vGPCR (vGPCR cells), with an IC50 of 30.078 µM. In addition, a significant decrease in cell viability was observed by neutral red staining (QUE 30 - 100 µM, p < 0.05). At a molecular level, F-actin stained with phalloidin showed disorganized actin filaments in the presence of QUE (30 µM), resembling an apoptotic event. Then, apoptosis induced by QUE (30 µM, 24 h) was revealed by annexin V/PI analysis (p < 0.01) and caspase-3 activity (p < 0.001). Secondly, tumors from vGPCR cells were induced in nude mice. After 15 days of tumor development, mice were treated with QUE (50 or 100 mg/kg/d) or PBS (as control) administered by IP injection three times a week for 30 days. The results showed that tumor progression was retarded in mice treated with QUE (100 mg/kg/d) compared to control (p < 0.001); whereas tumor weight was reduced by QUE (50 or 100 mg/kg/d) at the end of the test (p < 0.05 and p < 0.01, respectively). Neither kidney nor liver damage was observed by analyzing biochemical parameters in serum. In conclusion, this study suggests that QUE exhibits antineoplastic activity in both, an in vitro and in vivo model of Kaposi’s sarcoma.
Fil: Principe, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Tiburzi, Silvina Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: García, Betina Noemi. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Gumilar, Fernanda Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: González Pardo, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Materia
QUERCETINA
SARCOMA DE KAPOSI
ACTIVIDAD ANTINEOPLÁSICA
RATONES NUDE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/290057
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In vitro and in vivo evidence of the antineoplastic activity of quercetin against Kaposi’s sarcomaPrincipe, GabrielTiburzi, Silvina MabelGarcía, Betina NoemiGumilar, Fernanda AndreaGonzález Pardo, María VerónicaQUERCETINASARCOMA DE KAPOSIACTIVIDAD ANTINEOPLÁSICARATONES NUDEhttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Quercetin (QUE) is a natural flavonoid known to exhibit anticancer properties. Kaposi’s sarcoma is a viral cancer caused by the human herpesvirus-8, which expresses a constitutively activated G protein-coupled receptor (vGPCR) during its lytic phase, able to induce oncogenic modifications that lead to tumor development. The goal of this work was to explore the antineoplastic effect of QUE in an in vitro and in vivo model of Kaposi’s sarcoma. Firstly, the antiproliferative effect of QUE was determined by crystal violet technique at different concentrations of QUE (1 - 100 µM) or vehicle (DMSO) for 48 h in endothelial cells stably expressing the vGPCR (vGPCR cells), with an IC50 of 30.078 µM. In addition, a significant decrease in cell viability was observed by neutral red staining (QUE 30 - 100 µM, p < 0.05). At a molecular level, F-actin stained with phalloidin showed disorganized actin filaments in the presence of QUE (30 µM), resembling an apoptotic event. Then, apoptosis induced by QUE (30 µM, 24 h) was revealed by annexin V/PI analysis (p < 0.01) and caspase-3 activity (p < 0.001). Secondly, tumors from vGPCR cells were induced in nude mice. After 15 days of tumor development, mice were treated with QUE (50 or 100 mg/kg/d) or PBS (as control) administered by IP injection three times a week for 30 days. The results showed that tumor progression was retarded in mice treated with QUE (100 mg/kg/d) compared to control (p < 0.001); whereas tumor weight was reduced by QUE (50 or 100 mg/kg/d) at the end of the test (p < 0.05 and p < 0.01, respectively). Neither kidney nor liver damage was observed by analyzing biochemical parameters in serum. In conclusion, this study suggests that QUE exhibits antineoplastic activity in both, an in vitro and in vivo model of Kaposi’s sarcoma.Fil: Principe, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: Tiburzi, Silvina Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: García, Betina Noemi. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Gumilar, Fernanda Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaFil: González Pardo, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; ArgentinaLXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de LaboratorioMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de BiologíaAsociación Argentina de Farmacología ExperimentalAsociación Argentina de Ciencia y Tecnología de Animales de LaboratorioFundación Revista MedicinaDi Vita, Ethel2023info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/290057In vitro and in vivo evidence of the antineoplastic activity of quercetin against Kaposi’s sarcoma; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 91-911669-9106CONICET DigitalCONICETenghttps://saic.org.ar/evento/reunion-anual-2023/info:eu-repo/semantics/altIdentifier/url/https://saic.org.ar/revista-medicina/medicina-2023/Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T15:30:57Zoai:ri.conicet.gov.ar:11336/290057instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 15:30:57.669CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In vitro and in vivo evidence of the antineoplastic activity of quercetin against Kaposi’s sarcoma
title In vitro and in vivo evidence of the antineoplastic activity of quercetin against Kaposi’s sarcoma
spellingShingle In vitro and in vivo evidence of the antineoplastic activity of quercetin against Kaposi’s sarcoma
Principe, Gabriel
QUERCETINA
SARCOMA DE KAPOSI
ACTIVIDAD ANTINEOPLÁSICA
RATONES NUDE
title_short In vitro and in vivo evidence of the antineoplastic activity of quercetin against Kaposi’s sarcoma
title_full In vitro and in vivo evidence of the antineoplastic activity of quercetin against Kaposi’s sarcoma
title_fullStr In vitro and in vivo evidence of the antineoplastic activity of quercetin against Kaposi’s sarcoma
title_full_unstemmed In vitro and in vivo evidence of the antineoplastic activity of quercetin against Kaposi’s sarcoma
title_sort In vitro and in vivo evidence of the antineoplastic activity of quercetin against Kaposi’s sarcoma
dc.creator.none.fl_str_mv Principe, Gabriel
Tiburzi, Silvina Mabel
García, Betina Noemi
Gumilar, Fernanda Andrea
González Pardo, María Verónica
author Principe, Gabriel
author_facet Principe, Gabriel
Tiburzi, Silvina Mabel
García, Betina Noemi
Gumilar, Fernanda Andrea
González Pardo, María Verónica
author_role author
author2 Tiburzi, Silvina Mabel
García, Betina Noemi
Gumilar, Fernanda Andrea
González Pardo, María Verónica
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Di Vita, Ethel
dc.subject.none.fl_str_mv QUERCETINA
SARCOMA DE KAPOSI
ACTIVIDAD ANTINEOPLÁSICA
RATONES NUDE
topic QUERCETINA
SARCOMA DE KAPOSI
ACTIVIDAD ANTINEOPLÁSICA
RATONES NUDE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Quercetin (QUE) is a natural flavonoid known to exhibit anticancer properties. Kaposi’s sarcoma is a viral cancer caused by the human herpesvirus-8, which expresses a constitutively activated G protein-coupled receptor (vGPCR) during its lytic phase, able to induce oncogenic modifications that lead to tumor development. The goal of this work was to explore the antineoplastic effect of QUE in an in vitro and in vivo model of Kaposi’s sarcoma. Firstly, the antiproliferative effect of QUE was determined by crystal violet technique at different concentrations of QUE (1 - 100 µM) or vehicle (DMSO) for 48 h in endothelial cells stably expressing the vGPCR (vGPCR cells), with an IC50 of 30.078 µM. In addition, a significant decrease in cell viability was observed by neutral red staining (QUE 30 - 100 µM, p < 0.05). At a molecular level, F-actin stained with phalloidin showed disorganized actin filaments in the presence of QUE (30 µM), resembling an apoptotic event. Then, apoptosis induced by QUE (30 µM, 24 h) was revealed by annexin V/PI analysis (p < 0.01) and caspase-3 activity (p < 0.001). Secondly, tumors from vGPCR cells were induced in nude mice. After 15 days of tumor development, mice were treated with QUE (50 or 100 mg/kg/d) or PBS (as control) administered by IP injection three times a week for 30 days. The results showed that tumor progression was retarded in mice treated with QUE (100 mg/kg/d) compared to control (p < 0.001); whereas tumor weight was reduced by QUE (50 or 100 mg/kg/d) at the end of the test (p < 0.05 and p < 0.01, respectively). Neither kidney nor liver damage was observed by analyzing biochemical parameters in serum. In conclusion, this study suggests that QUE exhibits antineoplastic activity in both, an in vitro and in vivo model of Kaposi’s sarcoma.
Fil: Principe, Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: Tiburzi, Silvina Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: García, Betina Noemi. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Gumilar, Fernanda Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
Fil: González Pardo, María Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Ciencias Biológicas y Biomédicas del Sur. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Instituto de Ciencias Biológicas y Biomédicas del Sur; Argentina
LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
Mar del Plata
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina de Biología
Asociación Argentina de Farmacología Experimental
Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio
description Quercetin (QUE) is a natural flavonoid known to exhibit anticancer properties. Kaposi’s sarcoma is a viral cancer caused by the human herpesvirus-8, which expresses a constitutively activated G protein-coupled receptor (vGPCR) during its lytic phase, able to induce oncogenic modifications that lead to tumor development. The goal of this work was to explore the antineoplastic effect of QUE in an in vitro and in vivo model of Kaposi’s sarcoma. Firstly, the antiproliferative effect of QUE was determined by crystal violet technique at different concentrations of QUE (1 - 100 µM) or vehicle (DMSO) for 48 h in endothelial cells stably expressing the vGPCR (vGPCR cells), with an IC50 of 30.078 µM. In addition, a significant decrease in cell viability was observed by neutral red staining (QUE 30 - 100 µM, p < 0.05). At a molecular level, F-actin stained with phalloidin showed disorganized actin filaments in the presence of QUE (30 µM), resembling an apoptotic event. Then, apoptosis induced by QUE (30 µM, 24 h) was revealed by annexin V/PI analysis (p < 0.01) and caspase-3 activity (p < 0.001). Secondly, tumors from vGPCR cells were induced in nude mice. After 15 days of tumor development, mice were treated with QUE (50 or 100 mg/kg/d) or PBS (as control) administered by IP injection three times a week for 30 days. The results showed that tumor progression was retarded in mice treated with QUE (100 mg/kg/d) compared to control (p < 0.001); whereas tumor weight was reduced by QUE (50 or 100 mg/kg/d) at the end of the test (p < 0.05 and p < 0.01, respectively). Neither kidney nor liver damage was observed by analyzing biochemical parameters in serum. In conclusion, this study suggests that QUE exhibits antineoplastic activity in both, an in vitro and in vivo model of Kaposi’s sarcoma.
publishDate 2023
dc.date.none.fl_str_mv 2023
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info:eu-repo/semantics/conferenceObject
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Journal
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status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/290057
In vitro and in vivo evidence of the antineoplastic activity of quercetin against Kaposi’s sarcoma; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 91-91
1669-9106
CONICET Digital
CONICET
url http://hdl.handle.net/11336/290057
identifier_str_mv In vitro and in vivo evidence of the antineoplastic activity of quercetin against Kaposi’s sarcoma; LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica; XXV Jornadas Anuales de la Sociedad Argentina de Biología; LV Reunión Anual de la Asociación Argentina de Farmacología Experimental y VIII Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2023; 91-91
1669-9106
CONICET Digital
CONICET
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language eng
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publisher.none.fl_str_mv Fundación Revista Medicina
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