Leaf transcriptome differences between diploid and tetraploid bahiagrass

Autores
Podio, Maricel; Santoro, Danilo Fabrizio; Colono, Carolina Marta; Ortiz, Juan Pablo Amelio; Albertini, Emidio; Pessino, Silvina Claudia
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Polyploid individuals of the subtropical forage grass Paspalum notatum Flüggé (bahiagrass) exhibit distinct phenotypes, including apomixis, enhanced vigor, gigas effects, and increased stress tolerance. While apomixis-based breeding programs supported by molecular tools have improved agronomic traits such as growth habit, forage dry matter, and lipid profile, a genome-wide understanding of ploidy-induced transcriptomic changes is still lacking. In this study, we aimed to generate a comprehensive reference catalog of transcripts differentially expressed in the leaves of diploid and tetraploid individuals, characterize genome responses to polyploidy, and identify candidate genes for breeding. Our results reveal distinct transcriptomic profiles in polyploids, with significant impacts on development, redox homeostasis, and photosynthesis—patterns consistent with those observed in other species. Gene ontology enrichment analyses highlighted key categories related to stress responses and signaling pathways. We also identified critical breeding targets, including transcription factors and hormone-related genes. Co-expression network analysis uncovered 532 master regulators affected by genome doubling, with non-random distribution across the genome and hotspot clustering in specific chromosomes. Overall, our findings provide novel insights into the molecular consequences of polyploidy in P. notatum, offering a valuable resource for molecular breeding programs aimed at improving stress tolerance, vigor, and other desirable traits.
Fil: Podio, Maricel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Santoro, Danilo Fabrizio. Università di Perugia; Italia
Fil: Colono, Carolina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Albertini, Emidio. Università di Perugia; Italia
Fil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Materia
Paspalum notatum
Ploidy
Transcriptome
RNA-seq
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/286479
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/286479
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Leaf transcriptome differences between diploid and tetraploid bahiagrassPodio, MaricelSantoro, Danilo FabrizioColono, Carolina MartaOrtiz, Juan Pablo AmelioAlbertini, EmidioPessino, Silvina ClaudiaPaspalum notatumPloidyTranscriptomeRNA-seqhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Polyploid individuals of the subtropical forage grass Paspalum notatum Flüggé (bahiagrass) exhibit distinct phenotypes, including apomixis, enhanced vigor, gigas effects, and increased stress tolerance. While apomixis-based breeding programs supported by molecular tools have improved agronomic traits such as growth habit, forage dry matter, and lipid profile, a genome-wide understanding of ploidy-induced transcriptomic changes is still lacking. In this study, we aimed to generate a comprehensive reference catalog of transcripts differentially expressed in the leaves of diploid and tetraploid individuals, characterize genome responses to polyploidy, and identify candidate genes for breeding. Our results reveal distinct transcriptomic profiles in polyploids, with significant impacts on development, redox homeostasis, and photosynthesis—patterns consistent with those observed in other species. Gene ontology enrichment analyses highlighted key categories related to stress responses and signaling pathways. We also identified critical breeding targets, including transcription factors and hormone-related genes. Co-expression network analysis uncovered 532 master regulators affected by genome doubling, with non-random distribution across the genome and hotspot clustering in specific chromosomes. Overall, our findings provide novel insights into the molecular consequences of polyploidy in P. notatum, offering a valuable resource for molecular breeding programs aimed at improving stress tolerance, vigor, and other desirable traits.Fil: Podio, Maricel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Santoro, Danilo Fabrizio. Università di Perugia; ItaliaFil: Colono, Carolina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Albertini, Emidio. Università di Perugia; ItaliaFil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaJohn Wiley & Sons2026-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/286479Podio, Maricel; Santoro, Danilo Fabrizio; Colono, Carolina Marta; Ortiz, Juan Pablo Amelio; Albertini, Emidio; et al.; Leaf transcriptome differences between diploid and tetraploid bahiagrass; John Wiley & Sons; Plant Genome; 19; 1; 4-2026; 1-141940-3372CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/tpg2.70212info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:36:19Zoai:ri.conicet.gov.ar:11336/286479instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:36:20.74CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Leaf transcriptome differences between diploid and tetraploid bahiagrass
title Leaf transcriptome differences between diploid and tetraploid bahiagrass
spellingShingle Leaf transcriptome differences between diploid and tetraploid bahiagrass
Podio, Maricel
Paspalum notatum
Ploidy
Transcriptome
RNA-seq
title_short Leaf transcriptome differences between diploid and tetraploid bahiagrass
title_full Leaf transcriptome differences between diploid and tetraploid bahiagrass
title_fullStr Leaf transcriptome differences between diploid and tetraploid bahiagrass
title_full_unstemmed Leaf transcriptome differences between diploid and tetraploid bahiagrass
title_sort Leaf transcriptome differences between diploid and tetraploid bahiagrass
dc.creator.none.fl_str_mv Podio, Maricel
Santoro, Danilo Fabrizio
Colono, Carolina Marta
Ortiz, Juan Pablo Amelio
Albertini, Emidio
Pessino, Silvina Claudia
author Podio, Maricel
author_facet Podio, Maricel
Santoro, Danilo Fabrizio
Colono, Carolina Marta
Ortiz, Juan Pablo Amelio
Albertini, Emidio
Pessino, Silvina Claudia
author_role author
author2 Santoro, Danilo Fabrizio
Colono, Carolina Marta
Ortiz, Juan Pablo Amelio
Albertini, Emidio
Pessino, Silvina Claudia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Paspalum notatum
Ploidy
Transcriptome
RNA-seq
topic Paspalum notatum
Ploidy
Transcriptome
RNA-seq
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Polyploid individuals of the subtropical forage grass Paspalum notatum Flüggé (bahiagrass) exhibit distinct phenotypes, including apomixis, enhanced vigor, gigas effects, and increased stress tolerance. While apomixis-based breeding programs supported by molecular tools have improved agronomic traits such as growth habit, forage dry matter, and lipid profile, a genome-wide understanding of ploidy-induced transcriptomic changes is still lacking. In this study, we aimed to generate a comprehensive reference catalog of transcripts differentially expressed in the leaves of diploid and tetraploid individuals, characterize genome responses to polyploidy, and identify candidate genes for breeding. Our results reveal distinct transcriptomic profiles in polyploids, with significant impacts on development, redox homeostasis, and photosynthesis—patterns consistent with those observed in other species. Gene ontology enrichment analyses highlighted key categories related to stress responses and signaling pathways. We also identified critical breeding targets, including transcription factors and hormone-related genes. Co-expression network analysis uncovered 532 master regulators affected by genome doubling, with non-random distribution across the genome and hotspot clustering in specific chromosomes. Overall, our findings provide novel insights into the molecular consequences of polyploidy in P. notatum, offering a valuable resource for molecular breeding programs aimed at improving stress tolerance, vigor, and other desirable traits.
Fil: Podio, Maricel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Santoro, Danilo Fabrizio. Università di Perugia; Italia
Fil: Colono, Carolina Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Albertini, Emidio. Università di Perugia; Italia
Fil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
description Polyploid individuals of the subtropical forage grass Paspalum notatum Flüggé (bahiagrass) exhibit distinct phenotypes, including apomixis, enhanced vigor, gigas effects, and increased stress tolerance. While apomixis-based breeding programs supported by molecular tools have improved agronomic traits such as growth habit, forage dry matter, and lipid profile, a genome-wide understanding of ploidy-induced transcriptomic changes is still lacking. In this study, we aimed to generate a comprehensive reference catalog of transcripts differentially expressed in the leaves of diploid and tetraploid individuals, characterize genome responses to polyploidy, and identify candidate genes for breeding. Our results reveal distinct transcriptomic profiles in polyploids, with significant impacts on development, redox homeostasis, and photosynthesis—patterns consistent with those observed in other species. Gene ontology enrichment analyses highlighted key categories related to stress responses and signaling pathways. We also identified critical breeding targets, including transcription factors and hormone-related genes. Co-expression network analysis uncovered 532 master regulators affected by genome doubling, with non-random distribution across the genome and hotspot clustering in specific chromosomes. Overall, our findings provide novel insights into the molecular consequences of polyploidy in P. notatum, offering a valuable resource for molecular breeding programs aimed at improving stress tolerance, vigor, and other desirable traits.
publishDate 2026
dc.date.none.fl_str_mv 2026-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/286479
Podio, Maricel; Santoro, Danilo Fabrizio; Colono, Carolina Marta; Ortiz, Juan Pablo Amelio; Albertini, Emidio; et al.; Leaf transcriptome differences between diploid and tetraploid bahiagrass; John Wiley & Sons; Plant Genome; 19; 1; 4-2026; 1-14
1940-3372
CONICET Digital
CONICET
url http://hdl.handle.net/11336/286479
identifier_str_mv Podio, Maricel; Santoro, Danilo Fabrizio; Colono, Carolina Marta; Ortiz, Juan Pablo Amelio; Albertini, Emidio; et al.; Leaf transcriptome differences between diploid and tetraploid bahiagrass; John Wiley & Sons; Plant Genome; 19; 1; 4-2026; 1-14
1940-3372
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/tpg2.70212
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1874774291220791296
score 13.24418

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