Eutectozymes as Soft Hybrid Materials for Advanced Biocatalysis

Autores
Martinez Cartagena, Manuel Eduardo; Suarez, Lucia; Ontoria, Aitor; Benitez, Francisca J.; Rezabal, Elixabete; Orellano, María Soledad; Calderón, Marcelo; Huck Iriart, Cristián; Picco, Agustin Silvio; Picchio, Matías Luis; Beloqui, Ana
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The development of soft hybrid materials that combine structural integrity, biocompatibility, and catalytic function is a centralchallenge in advanced biocatalysis. Here, we introduce eutectozymes, enzyme-loaded eutectogels built from natural hydrophobicdeep eutectic solvents (HES) and stabilized through a dual supramolecular polymeric network. This unique architecture not onlypreserves the conformational integrity of enzymes but also creates confined microcavities acting as protective microreactors,thereby enhancing their stability and substrate affinity. Eutectozymes exhibit remarkable resilience under harsh operationalconditions, including high temperatures, extreme pH, and organic solvents, while maintaining high catalytic efficiency andreusability. Their versatility is further demonstrated in environmental remediation, achieving over 90% degradation of recalcitrantdyes, and in antimicrobial applications against resistant bacterial strains. By synergistically merging the stabilizing properties ofHES with robust gel matrices, eutectozymes establish a transformative platform for heterogeneous biocatalysis, opening newavenues in biotechnology, bioelectronics, and environmental technologies.
Fil: Martinez Cartagena, Manuel Eduardo. Universidad del Pais Vasco. Polymat.; España
Fil: Suarez, Lucia. Universidad del Pais Vasco. Polymat.; España
Fil: Ontoria, Aitor. Universidad del Pais Vasco. Polymat.; España
Fil: Benitez, Francisca J.. Donostia International Physic Center (dipc);
Fil: Rezabal, Elixabete. Donostia International Physic Center (dipc);
Fil: Orellano, María Soledad. Universidad del Pais Vasco. Polymat.; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Calderón, Marcelo. Universidad del Pais Vasco. Polymat.; España
Fil: Huck Iriart, Cristián. Alba - Synchrotron Light Source.; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Picco, Agustin Silvio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Picchio, Matías Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Pais Vasco. Polymat.; España
Fil: Beloqui, Ana. Universidad del Pais Vasco. Polymat.; España
Materia
BIOCATALYSIS
DEEP EUTECTIC SOLVENTS
ENZYME IMMOBILIZATION
EUTECTOGELS
EUTECTOZYMES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/291630
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/291630
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Eutectozymes as Soft Hybrid Materials for Advanced BiocatalysisMartinez Cartagena, Manuel EduardoSuarez, LuciaOntoria, AitorBenitez, Francisca J.Rezabal, ElixabeteOrellano, María SoledadCalderón, MarceloHuck Iriart, CristiánPicco, Agustin SilvioPicchio, Matías LuisBeloqui, AnaBIOCATALYSISDEEP EUTECTIC SOLVENTSENZYME IMMOBILIZATIONEUTECTOGELSEUTECTOZYMEShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The development of soft hybrid materials that combine structural integrity, biocompatibility, and catalytic function is a centralchallenge in advanced biocatalysis. Here, we introduce eutectozymes, enzyme-loaded eutectogels built from natural hydrophobicdeep eutectic solvents (HES) and stabilized through a dual supramolecular polymeric network. This unique architecture not onlypreserves the conformational integrity of enzymes but also creates confined microcavities acting as protective microreactors,thereby enhancing their stability and substrate affinity. Eutectozymes exhibit remarkable resilience under harsh operationalconditions, including high temperatures, extreme pH, and organic solvents, while maintaining high catalytic efficiency andreusability. Their versatility is further demonstrated in environmental remediation, achieving over 90% degradation of recalcitrantdyes, and in antimicrobial applications against resistant bacterial strains. By synergistically merging the stabilizing properties ofHES with robust gel matrices, eutectozymes establish a transformative platform for heterogeneous biocatalysis, opening newavenues in biotechnology, bioelectronics, and environmental technologies.Fil: Martinez Cartagena, Manuel Eduardo. Universidad del Pais Vasco. Polymat.; EspañaFil: Suarez, Lucia. Universidad del Pais Vasco. Polymat.; EspañaFil: Ontoria, Aitor. Universidad del Pais Vasco. Polymat.; EspañaFil: Benitez, Francisca J.. Donostia International Physic Center (dipc);Fil: Rezabal, Elixabete. Donostia International Physic Center (dipc);Fil: Orellano, María Soledad. Universidad del Pais Vasco. Polymat.; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Calderón, Marcelo. Universidad del Pais Vasco. Polymat.; EspañaFil: Huck Iriart, Cristián. Alba - Synchrotron Light Source.; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Picco, Agustin Silvio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; ArgentinaFil: Picchio, Matías Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Pais Vasco. Polymat.; EspañaFil: Beloqui, Ana. Universidad del Pais Vasco. Polymat.; EspañaWiley VCH Verlag2025-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/291630Martinez Cartagena, Manuel Eduardo; Suarez, Lucia; Ontoria, Aitor; Benitez, Francisca J.; Rezabal, Elixabete; et al.; Eutectozymes as Soft Hybrid Materials for Advanced Biocatalysis; Wiley VCH Verlag; Advanced Materials; 12-2025; 1-150935-9648CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://advanced.onlinelibrary.wiley.com/doi/10.1002/adma.202517014info:eu-repo/semantics/altIdentifier/doi/10.1002/adma.202517014info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T15:09:59Zoai:ri.conicet.gov.ar:11336/291630instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 15:10:00.175CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Eutectozymes as Soft Hybrid Materials for Advanced Biocatalysis
title Eutectozymes as Soft Hybrid Materials for Advanced Biocatalysis
spellingShingle Eutectozymes as Soft Hybrid Materials for Advanced Biocatalysis
Martinez Cartagena, Manuel Eduardo
BIOCATALYSIS
DEEP EUTECTIC SOLVENTS
ENZYME IMMOBILIZATION
EUTECTOGELS
EUTECTOZYMES
title_short Eutectozymes as Soft Hybrid Materials for Advanced Biocatalysis
title_full Eutectozymes as Soft Hybrid Materials for Advanced Biocatalysis
title_fullStr Eutectozymes as Soft Hybrid Materials for Advanced Biocatalysis
title_full_unstemmed Eutectozymes as Soft Hybrid Materials for Advanced Biocatalysis
title_sort Eutectozymes as Soft Hybrid Materials for Advanced Biocatalysis
dc.creator.none.fl_str_mv Martinez Cartagena, Manuel Eduardo
Suarez, Lucia
Ontoria, Aitor
Benitez, Francisca J.
Rezabal, Elixabete
Orellano, María Soledad
Calderón, Marcelo
Huck Iriart, Cristián
Picco, Agustin Silvio
Picchio, Matías Luis
Beloqui, Ana
author Martinez Cartagena, Manuel Eduardo
author_facet Martinez Cartagena, Manuel Eduardo
Suarez, Lucia
Ontoria, Aitor
Benitez, Francisca J.
Rezabal, Elixabete
Orellano, María Soledad
Calderón, Marcelo
Huck Iriart, Cristián
Picco, Agustin Silvio
Picchio, Matías Luis
Beloqui, Ana
author_role author
author2 Suarez, Lucia
Ontoria, Aitor
Benitez, Francisca J.
Rezabal, Elixabete
Orellano, María Soledad
Calderón, Marcelo
Huck Iriart, Cristián
Picco, Agustin Silvio
Picchio, Matías Luis
Beloqui, Ana
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BIOCATALYSIS
DEEP EUTECTIC SOLVENTS
ENZYME IMMOBILIZATION
EUTECTOGELS
EUTECTOZYMES
topic BIOCATALYSIS
DEEP EUTECTIC SOLVENTS
ENZYME IMMOBILIZATION
EUTECTOGELS
EUTECTOZYMES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The development of soft hybrid materials that combine structural integrity, biocompatibility, and catalytic function is a centralchallenge in advanced biocatalysis. Here, we introduce eutectozymes, enzyme-loaded eutectogels built from natural hydrophobicdeep eutectic solvents (HES) and stabilized through a dual supramolecular polymeric network. This unique architecture not onlypreserves the conformational integrity of enzymes but also creates confined microcavities acting as protective microreactors,thereby enhancing their stability and substrate affinity. Eutectozymes exhibit remarkable resilience under harsh operationalconditions, including high temperatures, extreme pH, and organic solvents, while maintaining high catalytic efficiency andreusability. Their versatility is further demonstrated in environmental remediation, achieving over 90% degradation of recalcitrantdyes, and in antimicrobial applications against resistant bacterial strains. By synergistically merging the stabilizing properties ofHES with robust gel matrices, eutectozymes establish a transformative platform for heterogeneous biocatalysis, opening newavenues in biotechnology, bioelectronics, and environmental technologies.
Fil: Martinez Cartagena, Manuel Eduardo. Universidad del Pais Vasco. Polymat.; España
Fil: Suarez, Lucia. Universidad del Pais Vasco. Polymat.; España
Fil: Ontoria, Aitor. Universidad del Pais Vasco. Polymat.; España
Fil: Benitez, Francisca J.. Donostia International Physic Center (dipc);
Fil: Rezabal, Elixabete. Donostia International Physic Center (dipc);
Fil: Orellano, María Soledad. Universidad del Pais Vasco. Polymat.; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Calderón, Marcelo. Universidad del Pais Vasco. Polymat.; España
Fil: Huck Iriart, Cristián. Alba - Synchrotron Light Source.; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Picco, Agustin Silvio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina
Fil: Picchio, Matías Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Pais Vasco. Polymat.; España
Fil: Beloqui, Ana. Universidad del Pais Vasco. Polymat.; España
description The development of soft hybrid materials that combine structural integrity, biocompatibility, and catalytic function is a centralchallenge in advanced biocatalysis. Here, we introduce eutectozymes, enzyme-loaded eutectogels built from natural hydrophobicdeep eutectic solvents (HES) and stabilized through a dual supramolecular polymeric network. This unique architecture not onlypreserves the conformational integrity of enzymes but also creates confined microcavities acting as protective microreactors,thereby enhancing their stability and substrate affinity. Eutectozymes exhibit remarkable resilience under harsh operationalconditions, including high temperatures, extreme pH, and organic solvents, while maintaining high catalytic efficiency andreusability. Their versatility is further demonstrated in environmental remediation, achieving over 90% degradation of recalcitrantdyes, and in antimicrobial applications against resistant bacterial strains. By synergistically merging the stabilizing properties ofHES with robust gel matrices, eutectozymes establish a transformative platform for heterogeneous biocatalysis, opening newavenues in biotechnology, bioelectronics, and environmental technologies.
publishDate 2025
dc.date.none.fl_str_mv 2025-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/291630
Martinez Cartagena, Manuel Eduardo; Suarez, Lucia; Ontoria, Aitor; Benitez, Francisca J.; Rezabal, Elixabete; et al.; Eutectozymes as Soft Hybrid Materials for Advanced Biocatalysis; Wiley VCH Verlag; Advanced Materials; 12-2025; 1-15
0935-9648
CONICET Digital
CONICET
url http://hdl.handle.net/11336/291630
identifier_str_mv Martinez Cartagena, Manuel Eduardo; Suarez, Lucia; Ontoria, Aitor; Benitez, Francisca J.; Rezabal, Elixabete; et al.; Eutectozymes as Soft Hybrid Materials for Advanced Biocatalysis; Wiley VCH Verlag; Advanced Materials; 12-2025; 1-15
0935-9648
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://advanced.onlinelibrary.wiley.com/doi/10.1002/adma.202517014
info:eu-repo/semantics/altIdentifier/doi/10.1002/adma.202517014
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.265058

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