Study of SRB adherence on carbon steel surfaces by electrochemical and microscopic techniques

Autores
Viera, Marisa Rosana; Rastelli, Silvia Elena; Gómez de Saravia, Sandra Gabriela
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
It is well known that microbiologically influenced corrosion (MIC) is a serious issue in the oil and gas industry. This process is caused by a mixture of microorganisms, including aerobic and anaerobic bacteria. Among the later, the Sulphate Reducing Bacteria (SRB) are often designated as the main responsible of the MIC in anaerobic environments such as those found in the oil extraction industry. Extracellular polymeric substances (EPS) produced by SRB have the ability to accelerate corrosion by binding with metal ions. The aim of this study was to evaluate the formation of biofilms of SRB on carbon steel surfaces by electrochemical and microscopic techniques. Anaerobic bacterial cultures used in the experiments were isolated from different systems that presented problems of MIC. Carbon steel coupons of SAE 1010 were placed in the cultures of different species of SRB to allow biofilm development. At various times, coupons were extracted and bacterial adherence was measured by viable SRB counts, epifluorescence microscopy and by crystal violet assay. The surface attack and biofilm morphology were analyzed by scanning electron microscopy (SEM). The corrosion of the carbon steel surface was monitored using electrochemical techniques: electrochemical impedance spectroscopy and corrosion potential measurements. Studies carried out allowed correlating the adherence and the EPS of the tested SRB strains with the different degree of attack suffered by the SAE 1010 carbon steel coupons.
Fil: Viera, Marisa Rosana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
Fil: Rastelli, Silvia Elena. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
Fil: Gómez de Saravia, Sandra Gabriela. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
17th International Biodegradation & Biodeterioration Symposium
Manchester
Reino Unido
Manchester Metropolitan University
Materia
MICROBIAL INFLUENCED CORROSION
CARBON STEEL
SULFATE REDUCING BACTERIA
SURFACE ATTACK
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/287095
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Study of SRB adherence on carbon steel surfaces by electrochemical and microscopic techniquesViera, Marisa RosanaRastelli, Silvia ElenaGómez de Saravia, Sandra GabrielaMICROBIAL INFLUENCED CORROSIONCARBON STEELSULFATE REDUCING BACTERIASURFACE ATTACKhttps://purl.org/becyt/ford/1.7https://purl.org/becyt/ford/1It is well known that microbiologically influenced corrosion (MIC) is a serious issue in the oil and gas industry. This process is caused by a mixture of microorganisms, including aerobic and anaerobic bacteria. Among the later, the Sulphate Reducing Bacteria (SRB) are often designated as the main responsible of the MIC in anaerobic environments such as those found in the oil extraction industry. Extracellular polymeric substances (EPS) produced by SRB have the ability to accelerate corrosion by binding with metal ions. The aim of this study was to evaluate the formation of biofilms of SRB on carbon steel surfaces by electrochemical and microscopic techniques. Anaerobic bacterial cultures used in the experiments were isolated from different systems that presented problems of MIC. Carbon steel coupons of SAE 1010 were placed in the cultures of different species of SRB to allow biofilm development. At various times, coupons were extracted and bacterial adherence was measured by viable SRB counts, epifluorescence microscopy and by crystal violet assay. The surface attack and biofilm morphology were analyzed by scanning electron microscopy (SEM). The corrosion of the carbon steel surface was monitored using electrochemical techniques: electrochemical impedance spectroscopy and corrosion potential measurements. Studies carried out allowed correlating the adherence and the EPS of the tested SRB strains with the different degree of attack suffered by the SAE 1010 carbon steel coupons.Fil: Viera, Marisa Rosana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; ArgentinaFil: Rastelli, Silvia Elena. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; ArgentinaFil: Gómez de Saravia, Sandra Gabriela. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina17th International Biodegradation & Biodeterioration SymposiumManchesterReino UnidoManchester Metropolitan UniversityManchester Metropolitan University2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectSimposioBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/287095Study of SRB adherence on carbon steel surfaces by electrochemical and microscopic techniques; 17th International Biodegradation & Biodeterioration Symposium; Manchester; Reino Unido; 2017; 94-94978-0-9926498-1-4CONICET DigitalCONICETengInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:30:36Zoai:ri.conicet.gov.ar:11336/287095instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:30:36.483CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Study of SRB adherence on carbon steel surfaces by electrochemical and microscopic techniques
title Study of SRB adherence on carbon steel surfaces by electrochemical and microscopic techniques
spellingShingle Study of SRB adherence on carbon steel surfaces by electrochemical and microscopic techniques
Viera, Marisa Rosana
MICROBIAL INFLUENCED CORROSION
CARBON STEEL
SULFATE REDUCING BACTERIA
SURFACE ATTACK
title_short Study of SRB adherence on carbon steel surfaces by electrochemical and microscopic techniques
title_full Study of SRB adherence on carbon steel surfaces by electrochemical and microscopic techniques
title_fullStr Study of SRB adherence on carbon steel surfaces by electrochemical and microscopic techniques
title_full_unstemmed Study of SRB adherence on carbon steel surfaces by electrochemical and microscopic techniques
title_sort Study of SRB adherence on carbon steel surfaces by electrochemical and microscopic techniques
dc.creator.none.fl_str_mv Viera, Marisa Rosana
Rastelli, Silvia Elena
Gómez de Saravia, Sandra Gabriela
author Viera, Marisa Rosana
author_facet Viera, Marisa Rosana
Rastelli, Silvia Elena
Gómez de Saravia, Sandra Gabriela
author_role author
author2 Rastelli, Silvia Elena
Gómez de Saravia, Sandra Gabriela
author2_role author
author
dc.subject.none.fl_str_mv MICROBIAL INFLUENCED CORROSION
CARBON STEEL
SULFATE REDUCING BACTERIA
SURFACE ATTACK
topic MICROBIAL INFLUENCED CORROSION
CARBON STEEL
SULFATE REDUCING BACTERIA
SURFACE ATTACK
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.7
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv It is well known that microbiologically influenced corrosion (MIC) is a serious issue in the oil and gas industry. This process is caused by a mixture of microorganisms, including aerobic and anaerobic bacteria. Among the later, the Sulphate Reducing Bacteria (SRB) are often designated as the main responsible of the MIC in anaerobic environments such as those found in the oil extraction industry. Extracellular polymeric substances (EPS) produced by SRB have the ability to accelerate corrosion by binding with metal ions. The aim of this study was to evaluate the formation of biofilms of SRB on carbon steel surfaces by electrochemical and microscopic techniques. Anaerobic bacterial cultures used in the experiments were isolated from different systems that presented problems of MIC. Carbon steel coupons of SAE 1010 were placed in the cultures of different species of SRB to allow biofilm development. At various times, coupons were extracted and bacterial adherence was measured by viable SRB counts, epifluorescence microscopy and by crystal violet assay. The surface attack and biofilm morphology were analyzed by scanning electron microscopy (SEM). The corrosion of the carbon steel surface was monitored using electrochemical techniques: electrochemical impedance spectroscopy and corrosion potential measurements. Studies carried out allowed correlating the adherence and the EPS of the tested SRB strains with the different degree of attack suffered by the SAE 1010 carbon steel coupons.
Fil: Viera, Marisa Rosana. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
Fil: Rastelli, Silvia Elena. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
Fil: Gómez de Saravia, Sandra Gabriela. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Tecnología de Pinturas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigaciones en Tecnología de Pinturas; Argentina
17th International Biodegradation & Biodeterioration Symposium
Manchester
Reino Unido
Manchester Metropolitan University
description It is well known that microbiologically influenced corrosion (MIC) is a serious issue in the oil and gas industry. This process is caused by a mixture of microorganisms, including aerobic and anaerobic bacteria. Among the later, the Sulphate Reducing Bacteria (SRB) are often designated as the main responsible of the MIC in anaerobic environments such as those found in the oil extraction industry. Extracellular polymeric substances (EPS) produced by SRB have the ability to accelerate corrosion by binding with metal ions. The aim of this study was to evaluate the formation of biofilms of SRB on carbon steel surfaces by electrochemical and microscopic techniques. Anaerobic bacterial cultures used in the experiments were isolated from different systems that presented problems of MIC. Carbon steel coupons of SAE 1010 were placed in the cultures of different species of SRB to allow biofilm development. At various times, coupons were extracted and bacterial adherence was measured by viable SRB counts, epifluorescence microscopy and by crystal violet assay. The surface attack and biofilm morphology were analyzed by scanning electron microscopy (SEM). The corrosion of the carbon steel surface was monitored using electrochemical techniques: electrochemical impedance spectroscopy and corrosion potential measurements. Studies carried out allowed correlating the adherence and the EPS of the tested SRB strains with the different degree of attack suffered by the SAE 1010 carbon steel coupons.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Simposio
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/287095
Study of SRB adherence on carbon steel surfaces by electrochemical and microscopic techniques; 17th International Biodegradation & Biodeterioration Symposium; Manchester; Reino Unido; 2017; 94-94
978-0-9926498-1-4
CONICET Digital
CONICET
url http://hdl.handle.net/11336/287095
identifier_str_mv Study of SRB adherence on carbon steel surfaces by electrochemical and microscopic techniques; 17th International Biodegradation & Biodeterioration Symposium; Manchester; Reino Unido; 2017; 94-94
978-0-9926498-1-4
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Manchester Metropolitan University
publisher.none.fl_str_mv Manchester Metropolitan University
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24418

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