Electrophysiological Insights Into Natural Phenylpropanoids: Effects of Trans-Cinnamaldehyde and Eugenol on Nicotinic Acetylcholine Receptors

Autores
Chrestia, Juan Facundo; Bouzat, Cecilia Beatriz; Hernando, Guillermina Silvana
Año de publicación
2024
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Natural extracts and essential oils, abundant in bioactive compounds, have long been used in traditional medicine and are increasingly recognized as a source of new drug candidates. Recent research has renewed interest in compounds from sources like Cinnamomum verum oil, particularly trans-cinnamaldehyde (TCA) and eugenol (EGN), due to their potential therapeutic effects. As multitarget compounds, natural compounds are especially intriguing for their interactions with neurotransmitter receptors, including nicotinic acetylcholine receptors (nAChRs), which are part of the Cys-loop receptor family. nAChRs are ion channels activated by acetylcholine, present in both the central and peripheral nervous systems, and are key players in muscle contraction, memory, and attention. This study aimed to examine the molecular effects of TCA and EGN on two types of mammalians nAChRs, both implicated in various pathological conditions. Using single- channel electrophysiological recordings, we observed that TCA exerts a negative modulatory effect on both α7 and muscular nAChRs. Specifically, TCA significantly reduces α7 receptor activity by lowering the frequency of activation episodes, without altering amplitude or open duration. In muscle-type nAChRs, both TCA and EGN lead to a concentration-dependent reduction in open channel duration in the micromolar range, accompanied by a shift to shorter durations in the main closed state. The modulatory effects observed underscore the pharmacological importance of nAChRs as therapeutic targets and highlight the potential therapeutic as well as adverse effects of TCA and EGN. Our findings contribute valuable insights into how these natural compounds affect Cys-loop receptors, an area that remains underexplored but could play a critical role in therapeutic strategies.
Fil: Chrestia, Juan Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Bouzat, Cecilia Beatriz. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LII Reunión Anual de la Sociedad Argentina de Biofísica
Bahía Blanca
Argentina
Sociedad Argentina de Biofísica
Universidad Nacional del Sur
Materia
NICOTINIC RECEPTOR
EUGENOL
TRANS-CINNAMALDEHYDE
PATCH-CLAMP
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/289827
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/289827
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Electrophysiological Insights Into Natural Phenylpropanoids: Effects of Trans-Cinnamaldehyde and Eugenol on Nicotinic Acetylcholine ReceptorsChrestia, Juan FacundoBouzat, Cecilia BeatrizHernando, Guillermina SilvanaNICOTINIC RECEPTOREUGENOLTRANS-CINNAMALDEHYDEPATCH-CLAMPhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Natural extracts and essential oils, abundant in bioactive compounds, have long been used in traditional medicine and are increasingly recognized as a source of new drug candidates. Recent research has renewed interest in compounds from sources like Cinnamomum verum oil, particularly trans-cinnamaldehyde (TCA) and eugenol (EGN), due to their potential therapeutic effects. As multitarget compounds, natural compounds are especially intriguing for their interactions with neurotransmitter receptors, including nicotinic acetylcholine receptors (nAChRs), which are part of the Cys-loop receptor family. nAChRs are ion channels activated by acetylcholine, present in both the central and peripheral nervous systems, and are key players in muscle contraction, memory, and attention. This study aimed to examine the molecular effects of TCA and EGN on two types of mammalians nAChRs, both implicated in various pathological conditions. Using single- channel electrophysiological recordings, we observed that TCA exerts a negative modulatory effect on both α7 and muscular nAChRs. Specifically, TCA significantly reduces α7 receptor activity by lowering the frequency of activation episodes, without altering amplitude or open duration. In muscle-type nAChRs, both TCA and EGN lead to a concentration-dependent reduction in open channel duration in the micromolar range, accompanied by a shift to shorter durations in the main closed state. The modulatory effects observed underscore the pharmacological importance of nAChRs as therapeutic targets and highlight the potential therapeutic as well as adverse effects of TCA and EGN. Our findings contribute valuable insights into how these natural compounds affect Cys-loop receptors, an area that remains underexplored but could play a critical role in therapeutic strategies.Fil: Chrestia, Juan Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Bouzat, Cecilia Beatriz. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaLII Reunión Anual de la Sociedad Argentina de BiofísicaBahía BlancaArgentinaSociedad Argentina de BiofísicaUniversidad Nacional del SurSociedad Argentina de BiofísicaAcierno, Juan PabloCelej, Maria SoledadVázquez, Diego EduardoRivas, Gabriela Leonor2024info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/289827Electrophysiological Insights Into Natural Phenylpropanoids: Effects of Trans-Cinnamaldehyde and Eugenol on Nicotinic Acetylcholine Receptors; LII Reunión Anual de la Sociedad Argentina de Biofísica; Bahía Blanca; Argentina; 2024; 49-49CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/congreso-2024/#resumenesInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:43:18Zoai:ri.conicet.gov.ar:11336/289827instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:43:19.242CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Electrophysiological Insights Into Natural Phenylpropanoids: Effects of Trans-Cinnamaldehyde and Eugenol on Nicotinic Acetylcholine Receptors
title Electrophysiological Insights Into Natural Phenylpropanoids: Effects of Trans-Cinnamaldehyde and Eugenol on Nicotinic Acetylcholine Receptors
spellingShingle Electrophysiological Insights Into Natural Phenylpropanoids: Effects of Trans-Cinnamaldehyde and Eugenol on Nicotinic Acetylcholine Receptors
Chrestia, Juan Facundo
NICOTINIC RECEPTOR
EUGENOL
TRANS-CINNAMALDEHYDE
PATCH-CLAMP
title_short Electrophysiological Insights Into Natural Phenylpropanoids: Effects of Trans-Cinnamaldehyde and Eugenol on Nicotinic Acetylcholine Receptors
title_full Electrophysiological Insights Into Natural Phenylpropanoids: Effects of Trans-Cinnamaldehyde and Eugenol on Nicotinic Acetylcholine Receptors
title_fullStr Electrophysiological Insights Into Natural Phenylpropanoids: Effects of Trans-Cinnamaldehyde and Eugenol on Nicotinic Acetylcholine Receptors
title_full_unstemmed Electrophysiological Insights Into Natural Phenylpropanoids: Effects of Trans-Cinnamaldehyde and Eugenol on Nicotinic Acetylcholine Receptors
title_sort Electrophysiological Insights Into Natural Phenylpropanoids: Effects of Trans-Cinnamaldehyde and Eugenol on Nicotinic Acetylcholine Receptors
dc.creator.none.fl_str_mv Chrestia, Juan Facundo
Bouzat, Cecilia Beatriz
Hernando, Guillermina Silvana
author Chrestia, Juan Facundo
author_facet Chrestia, Juan Facundo
Bouzat, Cecilia Beatriz
Hernando, Guillermina Silvana
author_role author
author2 Bouzat, Cecilia Beatriz
Hernando, Guillermina Silvana
author2_role author
author
dc.contributor.none.fl_str_mv Acierno, Juan Pablo
Celej, Maria Soledad
Vázquez, Diego Eduardo
Rivas, Gabriela Leonor
dc.subject.none.fl_str_mv NICOTINIC RECEPTOR
EUGENOL
TRANS-CINNAMALDEHYDE
PATCH-CLAMP
topic NICOTINIC RECEPTOR
EUGENOL
TRANS-CINNAMALDEHYDE
PATCH-CLAMP
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Natural extracts and essential oils, abundant in bioactive compounds, have long been used in traditional medicine and are increasingly recognized as a source of new drug candidates. Recent research has renewed interest in compounds from sources like Cinnamomum verum oil, particularly trans-cinnamaldehyde (TCA) and eugenol (EGN), due to their potential therapeutic effects. As multitarget compounds, natural compounds are especially intriguing for their interactions with neurotransmitter receptors, including nicotinic acetylcholine receptors (nAChRs), which are part of the Cys-loop receptor family. nAChRs are ion channels activated by acetylcholine, present in both the central and peripheral nervous systems, and are key players in muscle contraction, memory, and attention. This study aimed to examine the molecular effects of TCA and EGN on two types of mammalians nAChRs, both implicated in various pathological conditions. Using single- channel electrophysiological recordings, we observed that TCA exerts a negative modulatory effect on both α7 and muscular nAChRs. Specifically, TCA significantly reduces α7 receptor activity by lowering the frequency of activation episodes, without altering amplitude or open duration. In muscle-type nAChRs, both TCA and EGN lead to a concentration-dependent reduction in open channel duration in the micromolar range, accompanied by a shift to shorter durations in the main closed state. The modulatory effects observed underscore the pharmacological importance of nAChRs as therapeutic targets and highlight the potential therapeutic as well as adverse effects of TCA and EGN. Our findings contribute valuable insights into how these natural compounds affect Cys-loop receptors, an area that remains underexplored but could play a critical role in therapeutic strategies.
Fil: Chrestia, Juan Facundo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Bouzat, Cecilia Beatriz. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Hernando, Guillermina Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
LII Reunión Anual de la Sociedad Argentina de Biofísica
Bahía Blanca
Argentina
Sociedad Argentina de Biofísica
Universidad Nacional del Sur
description Natural extracts and essential oils, abundant in bioactive compounds, have long been used in traditional medicine and are increasingly recognized as a source of new drug candidates. Recent research has renewed interest in compounds from sources like Cinnamomum verum oil, particularly trans-cinnamaldehyde (TCA) and eugenol (EGN), due to their potential therapeutic effects. As multitarget compounds, natural compounds are especially intriguing for their interactions with neurotransmitter receptors, including nicotinic acetylcholine receptors (nAChRs), which are part of the Cys-loop receptor family. nAChRs are ion channels activated by acetylcholine, present in both the central and peripheral nervous systems, and are key players in muscle contraction, memory, and attention. This study aimed to examine the molecular effects of TCA and EGN on two types of mammalians nAChRs, both implicated in various pathological conditions. Using single- channel electrophysiological recordings, we observed that TCA exerts a negative modulatory effect on both α7 and muscular nAChRs. Specifically, TCA significantly reduces α7 receptor activity by lowering the frequency of activation episodes, without altering amplitude or open duration. In muscle-type nAChRs, both TCA and EGN lead to a concentration-dependent reduction in open channel duration in the micromolar range, accompanied by a shift to shorter durations in the main closed state. The modulatory effects observed underscore the pharmacological importance of nAChRs as therapeutic targets and highlight the potential therapeutic as well as adverse effects of TCA and EGN. Our findings contribute valuable insights into how these natural compounds affect Cys-loop receptors, an area that remains underexplored but could play a critical role in therapeutic strategies.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/289827
Electrophysiological Insights Into Natural Phenylpropanoids: Effects of Trans-Cinnamaldehyde and Eugenol on Nicotinic Acetylcholine Receptors; LII Reunión Anual de la Sociedad Argentina de Biofísica; Bahía Blanca; Argentina; 2024; 49-49
CONICET Digital
CONICET
url http://hdl.handle.net/11336/289827
identifier_str_mv Electrophysiological Insights Into Natural Phenylpropanoids: Effects of Trans-Cinnamaldehyde and Eugenol on Nicotinic Acetylcholine Receptors; LII Reunión Anual de la Sociedad Argentina de Biofísica; Bahía Blanca; Argentina; 2024; 49-49
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://biofisica.org.ar/congreso-2024/#resumenes
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Biofísica
publisher.none.fl_str_mv Sociedad Argentina de Biofísica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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