Analise fenotípica e genotípica da virulência de Staphylococcus spp. e de sua dispersão clonal como contribuição ao estudo da mastite bovina

Autores
Marques, Viviane F.; de Souza, Miliane M.S.; de Mendonça, Elaine C.I.; Abreu de Alencar, Tatiani; Rocha Pribul, Bruno; de O. Coelho, Shana de M.; Lasagno, Mirta Cristina; Reinoso, Elina Beatríz
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mastitis is an inflammation of one or more mammary glands caused mainly by bacte- ria, among which the genus Staphylococcus plays an important role. Bacteria belonging to this genus are known to express virulence factors which allow their persistence and spread in the host. This study aimed to evaluate the phenotypic and genotypic aspects of virulence factors in Staphylococci spp. isolates from bovine mastitis clinical cases. A total of 272 milk samples from 8 farms in the South-Fluminense region of Rio de Janei- ro were analyzed. The samples underwent conventional bacterial identification, yielding 250 Staphylococci spp. isolates. These were tested for the phenotypic detection of slime production by the microplate and Congo Red Agar methods. The hemolysins production, hemolytic synergism, caseinase and DNase production were also evaluated. The isolates were then assayed through the Polymerase Chain Reaction method to detect genes asso- ciated with virulence factors such as: capsule (cap5, cap8), fibronectin (fnbA, fnbB), slime (icaA, icaD) and hemolysins (hla e hlb). Regarding the number of isolates assessed, 58% (145/250) were identified as coagulase-negative Staphylococcus spp. and 42% (105/250) as coagulase-positive Staphylococcus spp. The latter comprised 36.2% (38/105) of isolates identified as S. aureus, 11.4% (12/105) as S. intermedius and 3.8% (4/105) belonging to the SIG group. The hemolisin production was not significant, whereas only 6,4% (16/250) produced alfa hemolysis, 4,8% (12/250) produced beta hemolysis and 1,6% (4/250) was able to produce both. Caseinase production was observed in 66.4% (166/250) and sli- me production assayed through the microplate method was positive in 76,8% (192/250). DNAse was detected in coagulase-negative Staphylococcus spp. (38/145) and in S. aureus (14/38). Low association between genetic detection of icaA (38/250) and icaD (54/250) and slime phenotypic expression (192/250) suggest that others genetic markers can be involved in this expression. Regarding gene amplification, the isolates did not show sig- nificant correlation between the genetic detection of icaA (38/250) and icaD (54/250) and slime production (192/250), indicating that other genetic markers may be involved in this trait expression. The frequency of the occurrence of the others studied genes was of 4% (10/250) for cap5 and cap8, 32,8% (82/250) for fnbA, 4,4% (11/250) for fnbB, 19,2% (48/250) for hla and 18% (45/250) for hlb. The major circulating strain profile on the farms encompassed slime and caseinase producer strains. The spaA gene was found in all of the S. aureus isolates, presenting varying amplicons sizes, with 300bp being the prevalent size. The amplification of the coa gene showed nine polymorphic variants, with 600bp being the prevalent amplicon. The agr gene was also detected in every S. aureus isolate, with an amplicon of 200 bp. It was noticed that the presence or absence of the virulence genes in this study were not correlated with the 6 distinct electrophoretic profiles obtained by PFGE.
Fil: Marques, Viviane F.. Universidade Federal Rural do Rio de Janeiro; Brasil;
Fil: de Souza, Miliane M.S.. Universidade Federal Rural do Rio de Janeiro; Brasil;
Fil: de Mendonça, Elaine C.I.. Universidade Federal Rural do Rio de Janeiro; Brasil;
Fil: Abreu de Alencar, Tatiani. Universidade Federal Rural do Rio de Janeiro; Brasil;
Fil: Rocha Pribul, Bruno. Universidade Federal Rural do Rio de Janeiro; Brasil;
Fil: de O. Coelho, Shana de M.. Universidade Federal Rural do Rio de Janeiro; Brasil;
Fil: Lasagno, Mirta Cristina. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia. Area de Bacteriologia; Argentina;
Fil: Reinoso, Elina Beatríz. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia; Argentina;
Materia
S aureus
MASTITIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/812
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/812
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Analise fenotípica e genotípica da virulência de Staphylococcus spp. e de sua dispersão clonal como contribuição ao estudo da mastite bovinaMarques, Viviane F.de Souza, Miliane M.S.de Mendonça, Elaine C.I.Abreu de Alencar, TatianiRocha Pribul, Brunode O. Coelho, Shana de M.Lasagno, Mirta CristinaReinoso, Elina BeatrízS aureusMASTITIShttps://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.6Mastitis is an inflammation of one or more mammary glands caused mainly by bacte- ria, among which the genus Staphylococcus plays an important role. Bacteria belonging to this genus are known to express virulence factors which allow their persistence and spread in the host. This study aimed to evaluate the phenotypic and genotypic aspects of virulence factors in Staphylococci spp. isolates from bovine mastitis clinical cases. A total of 272 milk samples from 8 farms in the South-Fluminense region of Rio de Janei- ro were analyzed. The samples underwent conventional bacterial identification, yielding 250 Staphylococci spp. isolates. These were tested for the phenotypic detection of slime production by the microplate and Congo Red Agar methods. The hemolysins production, hemolytic synergism, caseinase and DNase production were also evaluated. The isolates were then assayed through the Polymerase Chain Reaction method to detect genes asso- ciated with virulence factors such as: capsule (cap5, cap8), fibronectin (fnbA, fnbB), slime (icaA, icaD) and hemolysins (hla e hlb). Regarding the number of isolates assessed, 58% (145/250) were identified as coagulase-negative Staphylococcus spp. and 42% (105/250) as coagulase-positive Staphylococcus spp. The latter comprised 36.2% (38/105) of isolates identified as S. aureus, 11.4% (12/105) as S. intermedius and 3.8% (4/105) belonging to the SIG group. The hemolisin production was not significant, whereas only 6,4% (16/250) produced alfa hemolysis, 4,8% (12/250) produced beta hemolysis and 1,6% (4/250) was able to produce both. Caseinase production was observed in 66.4% (166/250) and sli- me production assayed through the microplate method was positive in 76,8% (192/250). DNAse was detected in coagulase-negative Staphylococcus spp. (38/145) and in S. aureus (14/38). Low association between genetic detection of icaA (38/250) and icaD (54/250) and slime phenotypic expression (192/250) suggest that others genetic markers can be involved in this expression. Regarding gene amplification, the isolates did not show sig- nificant correlation between the genetic detection of icaA (38/250) and icaD (54/250) and slime production (192/250), indicating that other genetic markers may be involved in this trait expression. The frequency of the occurrence of the others studied genes was of 4% (10/250) for cap5 and cap8, 32,8% (82/250) for fnbA, 4,4% (11/250) for fnbB, 19,2% (48/250) for hla and 18% (45/250) for hlb. The major circulating strain profile on the farms encompassed slime and caseinase producer strains. The spaA gene was found in all of the S. aureus isolates, presenting varying amplicons sizes, with 300bp being the prevalent size. The amplification of the coa gene showed nine polymorphic variants, with 600bp being the prevalent amplicon. The agr gene was also detected in every S. aureus isolate, with an amplicon of 200 bp. It was noticed that the presence or absence of the virulence genes in this study were not correlated with the 6 distinct electrophoretic profiles obtained by PFGE.Fil: Marques, Viviane F.. Universidade Federal Rural do Rio de Janeiro; Brasil;Fil: de Souza, Miliane M.S.. Universidade Federal Rural do Rio de Janeiro; Brasil;Fil: de Mendonça, Elaine C.I.. Universidade Federal Rural do Rio de Janeiro; Brasil;Fil: Abreu de Alencar, Tatiani. Universidade Federal Rural do Rio de Janeiro; Brasil;Fil: Rocha Pribul, Bruno. Universidade Federal Rural do Rio de Janeiro; Brasil;Fil: de O. Coelho, Shana de M.. Universidade Federal Rural do Rio de Janeiro; Brasil;Fil: Lasagno, Mirta Cristina. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia. Area de Bacteriologia; Argentina;Fil: Reinoso, Elina Beatríz. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia; Argentina;Revista Pesquisa Veterinaria Brasileira2013-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/812Marques, Viviane F.; de Souza, Miliane M.S.; de Mendonça, Elaine C.I.; Abreu de Alencar, Tatiani; Rocha Pribul, Bruno; et al.; Analise fenotípica e genotípica da virulência de Staphylococcus spp. e de sua dispersão clonal como contribuição ao estudo da mastite bovina; Revista Pesquisa Veterinaria Brasileira; Pesquisa Veterinária Brasileira; 33; 2; 4-2013; 161-1700100-736X1678-5150enginfo:eu-repo/semantics/altIdentifier/url/http://www.scielo.br/scielo.php?pid=S0100-736X2013000200005&script=sci_arttextinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T15:53:03Zoai:ri.conicet.gov.ar:11336/812instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 15:53:03.664CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Analise fenotípica e genotípica da virulência de Staphylococcus spp. e de sua dispersão clonal como contribuição ao estudo da mastite bovina
title Analise fenotípica e genotípica da virulência de Staphylococcus spp. e de sua dispersão clonal como contribuição ao estudo da mastite bovina
spellingShingle Analise fenotípica e genotípica da virulência de Staphylococcus spp. e de sua dispersão clonal como contribuição ao estudo da mastite bovina
Marques, Viviane F.
S aureus
MASTITIS
title_short Analise fenotípica e genotípica da virulência de Staphylococcus spp. e de sua dispersão clonal como contribuição ao estudo da mastite bovina
title_full Analise fenotípica e genotípica da virulência de Staphylococcus spp. e de sua dispersão clonal como contribuição ao estudo da mastite bovina
title_fullStr Analise fenotípica e genotípica da virulência de Staphylococcus spp. e de sua dispersão clonal como contribuição ao estudo da mastite bovina
title_full_unstemmed Analise fenotípica e genotípica da virulência de Staphylococcus spp. e de sua dispersão clonal como contribuição ao estudo da mastite bovina
title_sort Analise fenotípica e genotípica da virulência de Staphylococcus spp. e de sua dispersão clonal como contribuição ao estudo da mastite bovina
dc.creator.none.fl_str_mv Marques, Viviane F.
de Souza, Miliane M.S.
de Mendonça, Elaine C.I.
Abreu de Alencar, Tatiani
Rocha Pribul, Bruno
de O. Coelho, Shana de M.
Lasagno, Mirta Cristina
Reinoso, Elina Beatríz
author Marques, Viviane F.
author_facet Marques, Viviane F.
de Souza, Miliane M.S.
de Mendonça, Elaine C.I.
Abreu de Alencar, Tatiani
Rocha Pribul, Bruno
de O. Coelho, Shana de M.
Lasagno, Mirta Cristina
Reinoso, Elina Beatríz
author_role author
author2 de Souza, Miliane M.S.
de Mendonça, Elaine C.I.
Abreu de Alencar, Tatiani
Rocha Pribul, Bruno
de O. Coelho, Shana de M.
Lasagno, Mirta Cristina
Reinoso, Elina Beatríz
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv S aureus
MASTITIS
topic S aureus
MASTITIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.6
dc.description.none.fl_txt_mv Mastitis is an inflammation of one or more mammary glands caused mainly by bacte- ria, among which the genus Staphylococcus plays an important role. Bacteria belonging to this genus are known to express virulence factors which allow their persistence and spread in the host. This study aimed to evaluate the phenotypic and genotypic aspects of virulence factors in Staphylococci spp. isolates from bovine mastitis clinical cases. A total of 272 milk samples from 8 farms in the South-Fluminense region of Rio de Janei- ro were analyzed. The samples underwent conventional bacterial identification, yielding 250 Staphylococci spp. isolates. These were tested for the phenotypic detection of slime production by the microplate and Congo Red Agar methods. The hemolysins production, hemolytic synergism, caseinase and DNase production were also evaluated. The isolates were then assayed through the Polymerase Chain Reaction method to detect genes asso- ciated with virulence factors such as: capsule (cap5, cap8), fibronectin (fnbA, fnbB), slime (icaA, icaD) and hemolysins (hla e hlb). Regarding the number of isolates assessed, 58% (145/250) were identified as coagulase-negative Staphylococcus spp. and 42% (105/250) as coagulase-positive Staphylococcus spp. The latter comprised 36.2% (38/105) of isolates identified as S. aureus, 11.4% (12/105) as S. intermedius and 3.8% (4/105) belonging to the SIG group. The hemolisin production was not significant, whereas only 6,4% (16/250) produced alfa hemolysis, 4,8% (12/250) produced beta hemolysis and 1,6% (4/250) was able to produce both. Caseinase production was observed in 66.4% (166/250) and sli- me production assayed through the microplate method was positive in 76,8% (192/250). DNAse was detected in coagulase-negative Staphylococcus spp. (38/145) and in S. aureus (14/38). Low association between genetic detection of icaA (38/250) and icaD (54/250) and slime phenotypic expression (192/250) suggest that others genetic markers can be involved in this expression. Regarding gene amplification, the isolates did not show sig- nificant correlation between the genetic detection of icaA (38/250) and icaD (54/250) and slime production (192/250), indicating that other genetic markers may be involved in this trait expression. The frequency of the occurrence of the others studied genes was of 4% (10/250) for cap5 and cap8, 32,8% (82/250) for fnbA, 4,4% (11/250) for fnbB, 19,2% (48/250) for hla and 18% (45/250) for hlb. The major circulating strain profile on the farms encompassed slime and caseinase producer strains. The spaA gene was found in all of the S. aureus isolates, presenting varying amplicons sizes, with 300bp being the prevalent size. The amplification of the coa gene showed nine polymorphic variants, with 600bp being the prevalent amplicon. The agr gene was also detected in every S. aureus isolate, with an amplicon of 200 bp. It was noticed that the presence or absence of the virulence genes in this study were not correlated with the 6 distinct electrophoretic profiles obtained by PFGE.
Fil: Marques, Viviane F.. Universidade Federal Rural do Rio de Janeiro; Brasil;
Fil: de Souza, Miliane M.S.. Universidade Federal Rural do Rio de Janeiro; Brasil;
Fil: de Mendonça, Elaine C.I.. Universidade Federal Rural do Rio de Janeiro; Brasil;
Fil: Abreu de Alencar, Tatiani. Universidade Federal Rural do Rio de Janeiro; Brasil;
Fil: Rocha Pribul, Bruno. Universidade Federal Rural do Rio de Janeiro; Brasil;
Fil: de O. Coelho, Shana de M.. Universidade Federal Rural do Rio de Janeiro; Brasil;
Fil: Lasagno, Mirta Cristina. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia. Area de Bacteriologia; Argentina;
Fil: Reinoso, Elina Beatríz. Universidad Nacional de Rio Cuarto. Facultad de Cs.exactas Fisicoquimicas y Naturales. Departamento de Microbiologia E Inmunologia; Argentina;
description Mastitis is an inflammation of one or more mammary glands caused mainly by bacte- ria, among which the genus Staphylococcus plays an important role. Bacteria belonging to this genus are known to express virulence factors which allow their persistence and spread in the host. This study aimed to evaluate the phenotypic and genotypic aspects of virulence factors in Staphylococci spp. isolates from bovine mastitis clinical cases. A total of 272 milk samples from 8 farms in the South-Fluminense region of Rio de Janei- ro were analyzed. The samples underwent conventional bacterial identification, yielding 250 Staphylococci spp. isolates. These were tested for the phenotypic detection of slime production by the microplate and Congo Red Agar methods. The hemolysins production, hemolytic synergism, caseinase and DNase production were also evaluated. The isolates were then assayed through the Polymerase Chain Reaction method to detect genes asso- ciated with virulence factors such as: capsule (cap5, cap8), fibronectin (fnbA, fnbB), slime (icaA, icaD) and hemolysins (hla e hlb). Regarding the number of isolates assessed, 58% (145/250) were identified as coagulase-negative Staphylococcus spp. and 42% (105/250) as coagulase-positive Staphylococcus spp. The latter comprised 36.2% (38/105) of isolates identified as S. aureus, 11.4% (12/105) as S. intermedius and 3.8% (4/105) belonging to the SIG group. The hemolisin production was not significant, whereas only 6,4% (16/250) produced alfa hemolysis, 4,8% (12/250) produced beta hemolysis and 1,6% (4/250) was able to produce both. Caseinase production was observed in 66.4% (166/250) and sli- me production assayed through the microplate method was positive in 76,8% (192/250). DNAse was detected in coagulase-negative Staphylococcus spp. (38/145) and in S. aureus (14/38). Low association between genetic detection of icaA (38/250) and icaD (54/250) and slime phenotypic expression (192/250) suggest that others genetic markers can be involved in this expression. Regarding gene amplification, the isolates did not show sig- nificant correlation between the genetic detection of icaA (38/250) and icaD (54/250) and slime production (192/250), indicating that other genetic markers may be involved in this trait expression. The frequency of the occurrence of the others studied genes was of 4% (10/250) for cap5 and cap8, 32,8% (82/250) for fnbA, 4,4% (11/250) for fnbB, 19,2% (48/250) for hla and 18% (45/250) for hlb. The major circulating strain profile on the farms encompassed slime and caseinase producer strains. The spaA gene was found in all of the S. aureus isolates, presenting varying amplicons sizes, with 300bp being the prevalent size. The amplification of the coa gene showed nine polymorphic variants, with 600bp being the prevalent amplicon. The agr gene was also detected in every S. aureus isolate, with an amplicon of 200 bp. It was noticed that the presence or absence of the virulence genes in this study were not correlated with the 6 distinct electrophoretic profiles obtained by PFGE.
publishDate 2013
dc.date.none.fl_str_mv 2013-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/812
Marques, Viviane F.; de Souza, Miliane M.S.; de Mendonça, Elaine C.I.; Abreu de Alencar, Tatiani; Rocha Pribul, Bruno; et al.; Analise fenotípica e genotípica da virulência de Staphylococcus spp. e de sua dispersão clonal como contribuição ao estudo da mastite bovina; Revista Pesquisa Veterinaria Brasileira; Pesquisa Veterinária Brasileira; 33; 2; 4-2013; 161-170
0100-736X
1678-5150
url http://hdl.handle.net/11336/812
identifier_str_mv Marques, Viviane F.; de Souza, Miliane M.S.; de Mendonça, Elaine C.I.; Abreu de Alencar, Tatiani; Rocha Pribul, Bruno; et al.; Analise fenotípica e genotípica da virulência de Staphylococcus spp. e de sua dispersão clonal como contribuição ao estudo da mastite bovina; Revista Pesquisa Veterinaria Brasileira; Pesquisa Veterinária Brasileira; 33; 2; 4-2013; 161-170
0100-736X
1678-5150
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.scielo.br/scielo.php?pid=S0100-736X2013000200005&script=sci_arttext
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Revista Pesquisa Veterinaria Brasileira
publisher.none.fl_str_mv Revista Pesquisa Veterinaria Brasileira
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24418

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