Soluble and membrane associated adenylate cyclases participate in capacitation with hyaluronic acid in bovine spermatozoa

Autores
Fernandez, Silvina; Morado, Sergio Adrián; Cetica, Pablo Daniel; Cordoba, Mariana
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Hyaluronic acid (HA), a glycosaminoglycan present in the female reproductive tract, mediates sperm motility, maturation and capacitation. Two adenylate cyclases, soluble (sAC) and membrane-associated (mAC), isoenzymes are present in spermatozoa, participating in different signaling mechanisms. The aim of this study was to determine the effect of adenylate cyclase isoenzymes inhibition on bovine sperm processes, such as capacitation, motility, viability, mitochondrial activity and fertilizing capacity, during sperm capacitation with HA. HA was used as a capacitation inducer, while LRE-1 (a specific sAC inhibitor) and 2,5-dideoxyadenosine (2,5-D) were used as sAC and mAC inhibitors, respectively. We evaluated sperm capacitation (chlortetracycline [CTC] technique), viability (trypan blue stain and differential interference contrast), mitochondrial activity (fluorochrome 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide [JC-1]) and motility (computer-assisted sperm analysis). We carried out in vitro fertilization (IVF) in IVF- modified synthetic oviductal fluid (mSOF) with frozen-thawed semen. We then cultured presumptive zygotes for 48 hours and analysed the cleavage rate. The addition of sAC or mAC inhibitors, alone or combined, produced a significant decrease in capacitation, mitochondrial activity and motility with respect to sperm samples incubated only with HA and controls (P < 0.05). Cleavage rates decreased with the addition of adeylate cyclase (AC) inhibitors compared to HA alone. We observed a greater decrease in cleavage rate with the addition of LRE-1 than with 2,5-D (P < 0.05). The combination of both inhibitors decreased the cleavage rate with respect to each inhibitor alone (P < 0.05). In bovine spermatozoa, both soluble and membrane-associated adenylate cyclase isoenzymes could participate in the intracellular signaling mechanism and mitochondrial function, which modulate HA capacitation and sperm fertilizing ability.
Fil: Fernandez, Silvina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Fil: Morado, Sergio Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Cetica, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Cordoba, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Materia
HYALUONIC ACID
ADENYLATE CYCLASE
CAPACITATION
IN VITRO FERTILIZATION
CATTLE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/290124
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/290124
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Soluble and membrane associated adenylate cyclases participate in capacitation with hyaluronic acid in bovine spermatozoaFernandez, SilvinaMorado, Sergio AdriánCetica, Pablo DanielCordoba, MarianaHYALUONIC ACIDADENYLATE CYCLASECAPACITATIONIN VITRO FERTILIZATIONCATTLEhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Hyaluronic acid (HA), a glycosaminoglycan present in the female reproductive tract, mediates sperm motility, maturation and capacitation. Two adenylate cyclases, soluble (sAC) and membrane-associated (mAC), isoenzymes are present in spermatozoa, participating in different signaling mechanisms. The aim of this study was to determine the effect of adenylate cyclase isoenzymes inhibition on bovine sperm processes, such as capacitation, motility, viability, mitochondrial activity and fertilizing capacity, during sperm capacitation with HA. HA was used as a capacitation inducer, while LRE-1 (a specific sAC inhibitor) and 2,5-dideoxyadenosine (2,5-D) were used as sAC and mAC inhibitors, respectively. We evaluated sperm capacitation (chlortetracycline [CTC] technique), viability (trypan blue stain and differential interference contrast), mitochondrial activity (fluorochrome 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide [JC-1]) and motility (computer-assisted sperm analysis). We carried out in vitro fertilization (IVF) in IVF- modified synthetic oviductal fluid (mSOF) with frozen-thawed semen. We then cultured presumptive zygotes for 48 hours and analysed the cleavage rate. The addition of sAC or mAC inhibitors, alone or combined, produced a significant decrease in capacitation, mitochondrial activity and motility with respect to sperm samples incubated only with HA and controls (P < 0.05). Cleavage rates decreased with the addition of adeylate cyclase (AC) inhibitors compared to HA alone. We observed a greater decrease in cleavage rate with the addition of LRE-1 than with 2,5-D (P < 0.05). The combination of both inhibitors decreased the cleavage rate with respect to each inhibitor alone (P < 0.05). In bovine spermatozoa, both soluble and membrane-associated adenylate cyclase isoenzymes could participate in the intracellular signaling mechanism and mitochondrial function, which modulate HA capacitation and sperm fertilizing ability.Fil: Fernandez, Silvina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; ArgentinaFil: Morado, Sergio Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Cetica, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaFil: Cordoba, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; ArgentinaUniversidad Nacional Autónoma de México. Facultad de Medicina Veterinaria y Zootecnia2026-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/290124Fernandez, Silvina; Morado, Sergio Adrián; Cetica, Pablo Daniel; Cordoba, Mariana; Soluble and membrane associated adenylate cyclases participate in capacitation with hyaluronic acid in bovine spermatozoa; Universidad Nacional Autónoma de México. Facultad de Medicina Veterinaria y Zootecnia; Veterinaria México OA; 13; 3-2026; 1-272448-6760CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://veterinariamexico.fmvz.unam.mx/index.php/vet/article/view/1531info:eu-repo/semantics/altIdentifier/doi/10.22201/fmvz.24486760e.2026.1531info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T15:42:09Zoai:ri.conicet.gov.ar:11336/290124instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 15:42:10.297CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Soluble and membrane associated adenylate cyclases participate in capacitation with hyaluronic acid in bovine spermatozoa
title Soluble and membrane associated adenylate cyclases participate in capacitation with hyaluronic acid in bovine spermatozoa
spellingShingle Soluble and membrane associated adenylate cyclases participate in capacitation with hyaluronic acid in bovine spermatozoa
Fernandez, Silvina
HYALUONIC ACID
ADENYLATE CYCLASE
CAPACITATION
IN VITRO FERTILIZATION
CATTLE
title_short Soluble and membrane associated adenylate cyclases participate in capacitation with hyaluronic acid in bovine spermatozoa
title_full Soluble and membrane associated adenylate cyclases participate in capacitation with hyaluronic acid in bovine spermatozoa
title_fullStr Soluble and membrane associated adenylate cyclases participate in capacitation with hyaluronic acid in bovine spermatozoa
title_full_unstemmed Soluble and membrane associated adenylate cyclases participate in capacitation with hyaluronic acid in bovine spermatozoa
title_sort Soluble and membrane associated adenylate cyclases participate in capacitation with hyaluronic acid in bovine spermatozoa
dc.creator.none.fl_str_mv Fernandez, Silvina
Morado, Sergio Adrián
Cetica, Pablo Daniel
Cordoba, Mariana
author Fernandez, Silvina
author_facet Fernandez, Silvina
Morado, Sergio Adrián
Cetica, Pablo Daniel
Cordoba, Mariana
author_role author
author2 Morado, Sergio Adrián
Cetica, Pablo Daniel
Cordoba, Mariana
author2_role author
author
author
dc.subject.none.fl_str_mv HYALUONIC ACID
ADENYLATE CYCLASE
CAPACITATION
IN VITRO FERTILIZATION
CATTLE
topic HYALUONIC ACID
ADENYLATE CYCLASE
CAPACITATION
IN VITRO FERTILIZATION
CATTLE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Hyaluronic acid (HA), a glycosaminoglycan present in the female reproductive tract, mediates sperm motility, maturation and capacitation. Two adenylate cyclases, soluble (sAC) and membrane-associated (mAC), isoenzymes are present in spermatozoa, participating in different signaling mechanisms. The aim of this study was to determine the effect of adenylate cyclase isoenzymes inhibition on bovine sperm processes, such as capacitation, motility, viability, mitochondrial activity and fertilizing capacity, during sperm capacitation with HA. HA was used as a capacitation inducer, while LRE-1 (a specific sAC inhibitor) and 2,5-dideoxyadenosine (2,5-D) were used as sAC and mAC inhibitors, respectively. We evaluated sperm capacitation (chlortetracycline [CTC] technique), viability (trypan blue stain and differential interference contrast), mitochondrial activity (fluorochrome 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide [JC-1]) and motility (computer-assisted sperm analysis). We carried out in vitro fertilization (IVF) in IVF- modified synthetic oviductal fluid (mSOF) with frozen-thawed semen. We then cultured presumptive zygotes for 48 hours and analysed the cleavage rate. The addition of sAC or mAC inhibitors, alone or combined, produced a significant decrease in capacitation, mitochondrial activity and motility with respect to sperm samples incubated only with HA and controls (P < 0.05). Cleavage rates decreased with the addition of adeylate cyclase (AC) inhibitors compared to HA alone. We observed a greater decrease in cleavage rate with the addition of LRE-1 than with 2,5-D (P < 0.05). The combination of both inhibitors decreased the cleavage rate with respect to each inhibitor alone (P < 0.05). In bovine spermatozoa, both soluble and membrane-associated adenylate cyclase isoenzymes could participate in the intracellular signaling mechanism and mitochondrial function, which modulate HA capacitation and sperm fertilizing ability.
Fil: Fernandez, Silvina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Instituto de Investigacion y Tecnología en Reproducción Animal; Argentina
Fil: Morado, Sergio Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Cetica, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
Fil: Cordoba, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
description Hyaluronic acid (HA), a glycosaminoglycan present in the female reproductive tract, mediates sperm motility, maturation and capacitation. Two adenylate cyclases, soluble (sAC) and membrane-associated (mAC), isoenzymes are present in spermatozoa, participating in different signaling mechanisms. The aim of this study was to determine the effect of adenylate cyclase isoenzymes inhibition on bovine sperm processes, such as capacitation, motility, viability, mitochondrial activity and fertilizing capacity, during sperm capacitation with HA. HA was used as a capacitation inducer, while LRE-1 (a specific sAC inhibitor) and 2,5-dideoxyadenosine (2,5-D) were used as sAC and mAC inhibitors, respectively. We evaluated sperm capacitation (chlortetracycline [CTC] technique), viability (trypan blue stain and differential interference contrast), mitochondrial activity (fluorochrome 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide [JC-1]) and motility (computer-assisted sperm analysis). We carried out in vitro fertilization (IVF) in IVF- modified synthetic oviductal fluid (mSOF) with frozen-thawed semen. We then cultured presumptive zygotes for 48 hours and analysed the cleavage rate. The addition of sAC or mAC inhibitors, alone or combined, produced a significant decrease in capacitation, mitochondrial activity and motility with respect to sperm samples incubated only with HA and controls (P < 0.05). Cleavage rates decreased with the addition of adeylate cyclase (AC) inhibitors compared to HA alone. We observed a greater decrease in cleavage rate with the addition of LRE-1 than with 2,5-D (P < 0.05). The combination of both inhibitors decreased the cleavage rate with respect to each inhibitor alone (P < 0.05). In bovine spermatozoa, both soluble and membrane-associated adenylate cyclase isoenzymes could participate in the intracellular signaling mechanism and mitochondrial function, which modulate HA capacitation and sperm fertilizing ability.
publishDate 2026
dc.date.none.fl_str_mv 2026-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/290124
Fernandez, Silvina; Morado, Sergio Adrián; Cetica, Pablo Daniel; Cordoba, Mariana; Soluble and membrane associated adenylate cyclases participate in capacitation with hyaluronic acid in bovine spermatozoa; Universidad Nacional Autónoma de México. Facultad de Medicina Veterinaria y Zootecnia; Veterinaria México OA; 13; 3-2026; 1-27
2448-6760
CONICET Digital
CONICET
url http://hdl.handle.net/11336/290124
identifier_str_mv Fernandez, Silvina; Morado, Sergio Adrián; Cetica, Pablo Daniel; Cordoba, Mariana; Soluble and membrane associated adenylate cyclases participate in capacitation with hyaluronic acid in bovine spermatozoa; Universidad Nacional Autónoma de México. Facultad de Medicina Veterinaria y Zootecnia; Veterinaria México OA; 13; 3-2026; 1-27
2448-6760
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://veterinariamexico.fmvz.unam.mx/index.php/vet/article/view/1531
info:eu-repo/semantics/altIdentifier/doi/10.22201/fmvz.24486760e.2026.1531
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Autónoma de México. Facultad de Medicina Veterinaria y Zootecnia
publisher.none.fl_str_mv Universidad Nacional Autónoma de México. Facultad de Medicina Veterinaria y Zootecnia
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.265058

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