Binge-like ethanol induced anxiety- like behavior, cell degeneration and possible amelioration of Omega-3 (ω-3) Fatty Acids in adolescent rats

Autores
Cabrera, Valentin; Savino, Luciana Victoria; Marenchino Gallo, Luana; Heredia Di Natale, Tomás; Abate, Paula; Balaszczuk, Veronica; Macchione, Ana Fabiola
Año de publicación
2025
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Alcohol consumption contributes to ∼5% of the global disease burden, with adolescents being particularly vulnerable. High alcohol exposure induces anxiety-like behavior and neuronal death, potentially through neuroinflammation and oxidative stress–induced apoptosis. Omega-3 (ω-3) fatty acids may counteract these effects through their anti-inflammatory and antioxidant properties. However, little is known about the long-term effects of ethanol (EtOH) exposure and the potential protective ω-3 effects in adolescents. Here, we evaluated the impact of EtOH exposure and the neuroprotective role of ω-3 on anxiety-like behavior and pyknotic cell number, in adolescent rats. Animals received 2 or 0 g/kg of EtOH (ig) on postnatal days (PDs) 28, 30, and 32 and, fifteen min later, they were administered with ω-3 (720 or 0 mg/kg, ig). On PD 34, animals were tested in the elevated plus maze for 5 min and were sacrificed for brain tissue collection. Pyknotic cells were stained with toluidine blue and quantified in the central amygdala (CeA). EtOH-treated animals showed an anxiogenic profile, spending less time in open arms; a profile that was improved by ω-3 (EtOH+ω-3 animals increase the time in open arms). EtOH significantly increased the pyknotic cell number in the CeA. These findings highlight ethanol´s long-term neurotoxic effects on cell degeneration and anxiety and provide evidence of possible amelioration of ω-3 in adolescents.
Fil: Cabrera, Valentin. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina
Fil: Savino, Luciana Victoria. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina
Fil: Marenchino Gallo, Luana. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina
Fil: Heredia Di Natale, Tomás. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina
Fil: Abate, Paula. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina
Fil: Balaszczuk, Veronica. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina
Fil: Macchione, Ana Fabiola. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina
XL SAN Meeting
Buenos Aires
Argentina
Sociedad Argentina de Investigación en Neurociencias
Materia
Alcohol
Omega-3
Anxiety
Cell Degeneration
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/290721
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Binge-like ethanol induced anxiety- like behavior, cell degeneration and possible amelioration of Omega-3 (ω-3) Fatty Acids in adolescent ratsCabrera, ValentinSavino, Luciana VictoriaMarenchino Gallo, LuanaHeredia Di Natale, TomásAbate, PaulaBalaszczuk, VeronicaMacchione, Ana FabiolaAlcoholOmega-3AnxietyCell Degenerationhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Alcohol consumption contributes to ∼5% of the global disease burden, with adolescents being particularly vulnerable. High alcohol exposure induces anxiety-like behavior and neuronal death, potentially through neuroinflammation and oxidative stress–induced apoptosis. Omega-3 (ω-3) fatty acids may counteract these effects through their anti-inflammatory and antioxidant properties. However, little is known about the long-term effects of ethanol (EtOH) exposure and the potential protective ω-3 effects in adolescents. Here, we evaluated the impact of EtOH exposure and the neuroprotective role of ω-3 on anxiety-like behavior and pyknotic cell number, in adolescent rats. Animals received 2 or 0 g/kg of EtOH (ig) on postnatal days (PDs) 28, 30, and 32 and, fifteen min later, they were administered with ω-3 (720 or 0 mg/kg, ig). On PD 34, animals were tested in the elevated plus maze for 5 min and were sacrificed for brain tissue collection. Pyknotic cells were stained with toluidine blue and quantified in the central amygdala (CeA). EtOH-treated animals showed an anxiogenic profile, spending less time in open arms; a profile that was improved by ω-3 (EtOH+ω-3 animals increase the time in open arms). EtOH significantly increased the pyknotic cell number in the CeA. These findings highlight ethanol´s long-term neurotoxic effects on cell degeneration and anxiety and provide evidence of possible amelioration of ω-3 in adolescents.Fil: Cabrera, Valentin. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; ArgentinaFil: Savino, Luciana Victoria. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; ArgentinaFil: Marenchino Gallo, Luana. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; ArgentinaFil: Heredia Di Natale, Tomás. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; ArgentinaFil: Abate, Paula. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; ArgentinaFil: Balaszczuk, Veronica. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; ArgentinaFil: Macchione, Ana Fabiola. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; ArgentinaXL SAN MeetingBuenos AiresArgentinaSociedad Argentina de Investigación en NeurocienciasSociedad Argentina de Investigación en Neurociencias2025info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectEncuentroBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/290721Binge-like ethanol induced anxiety- like behavior, cell degeneration and possible amelioration of Omega-3 (ω-3) Fatty Acids in adolescent rats; XL SAN Meeting; Buenos Aires; Argentina; 2025; 67-67CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://csan2025.saneurociencias.org.ar/poster/binge-like-ethanol-induced-anxiety-like-behavior-cell-degeneration-and-possible-amelioration-of-omega-3-%CF%89-3-fatty-acids-in-adolescent-rats/info:eu-repo/semantics/altIdentifier/url/https://csan2025.saneurociencias.org.ar/wp-content/uploads/2025/10/SAN2025-Posters-EBOOK-Final-1.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:47:02Zoai:ri.conicet.gov.ar:11336/290721instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:47:02.893CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Binge-like ethanol induced anxiety- like behavior, cell degeneration and possible amelioration of Omega-3 (ω-3) Fatty Acids in adolescent rats
title Binge-like ethanol induced anxiety- like behavior, cell degeneration and possible amelioration of Omega-3 (ω-3) Fatty Acids in adolescent rats
spellingShingle Binge-like ethanol induced anxiety- like behavior, cell degeneration and possible amelioration of Omega-3 (ω-3) Fatty Acids in adolescent rats
Cabrera, Valentin
Alcohol
Omega-3
Anxiety
Cell Degeneration
title_short Binge-like ethanol induced anxiety- like behavior, cell degeneration and possible amelioration of Omega-3 (ω-3) Fatty Acids in adolescent rats
title_full Binge-like ethanol induced anxiety- like behavior, cell degeneration and possible amelioration of Omega-3 (ω-3) Fatty Acids in adolescent rats
title_fullStr Binge-like ethanol induced anxiety- like behavior, cell degeneration and possible amelioration of Omega-3 (ω-3) Fatty Acids in adolescent rats
title_full_unstemmed Binge-like ethanol induced anxiety- like behavior, cell degeneration and possible amelioration of Omega-3 (ω-3) Fatty Acids in adolescent rats
title_sort Binge-like ethanol induced anxiety- like behavior, cell degeneration and possible amelioration of Omega-3 (ω-3) Fatty Acids in adolescent rats
dc.creator.none.fl_str_mv Cabrera, Valentin
Savino, Luciana Victoria
Marenchino Gallo, Luana
Heredia Di Natale, Tomás
Abate, Paula
Balaszczuk, Veronica
Macchione, Ana Fabiola
author Cabrera, Valentin
author_facet Cabrera, Valentin
Savino, Luciana Victoria
Marenchino Gallo, Luana
Heredia Di Natale, Tomás
Abate, Paula
Balaszczuk, Veronica
Macchione, Ana Fabiola
author_role author
author2 Savino, Luciana Victoria
Marenchino Gallo, Luana
Heredia Di Natale, Tomás
Abate, Paula
Balaszczuk, Veronica
Macchione, Ana Fabiola
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Alcohol
Omega-3
Anxiety
Cell Degeneration
topic Alcohol
Omega-3
Anxiety
Cell Degeneration
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Alcohol consumption contributes to ∼5% of the global disease burden, with adolescents being particularly vulnerable. High alcohol exposure induces anxiety-like behavior and neuronal death, potentially through neuroinflammation and oxidative stress–induced apoptosis. Omega-3 (ω-3) fatty acids may counteract these effects through their anti-inflammatory and antioxidant properties. However, little is known about the long-term effects of ethanol (EtOH) exposure and the potential protective ω-3 effects in adolescents. Here, we evaluated the impact of EtOH exposure and the neuroprotective role of ω-3 on anxiety-like behavior and pyknotic cell number, in adolescent rats. Animals received 2 or 0 g/kg of EtOH (ig) on postnatal days (PDs) 28, 30, and 32 and, fifteen min later, they were administered with ω-3 (720 or 0 mg/kg, ig). On PD 34, animals were tested in the elevated plus maze for 5 min and were sacrificed for brain tissue collection. Pyknotic cells were stained with toluidine blue and quantified in the central amygdala (CeA). EtOH-treated animals showed an anxiogenic profile, spending less time in open arms; a profile that was improved by ω-3 (EtOH+ω-3 animals increase the time in open arms). EtOH significantly increased the pyknotic cell number in the CeA. These findings highlight ethanol´s long-term neurotoxic effects on cell degeneration and anxiety and provide evidence of possible amelioration of ω-3 in adolescents.
Fil: Cabrera, Valentin. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina
Fil: Savino, Luciana Victoria. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina
Fil: Marenchino Gallo, Luana. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina
Fil: Heredia Di Natale, Tomás. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina
Fil: Abate, Paula. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina
Fil: Balaszczuk, Veronica. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina
Fil: Macchione, Ana Fabiola. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina
XL SAN Meeting
Buenos Aires
Argentina
Sociedad Argentina de Investigación en Neurociencias
description Alcohol consumption contributes to ∼5% of the global disease burden, with adolescents being particularly vulnerable. High alcohol exposure induces anxiety-like behavior and neuronal death, potentially through neuroinflammation and oxidative stress–induced apoptosis. Omega-3 (ω-3) fatty acids may counteract these effects through their anti-inflammatory and antioxidant properties. However, little is known about the long-term effects of ethanol (EtOH) exposure and the potential protective ω-3 effects in adolescents. Here, we evaluated the impact of EtOH exposure and the neuroprotective role of ω-3 on anxiety-like behavior and pyknotic cell number, in adolescent rats. Animals received 2 or 0 g/kg of EtOH (ig) on postnatal days (PDs) 28, 30, and 32 and, fifteen min later, they were administered with ω-3 (720 or 0 mg/kg, ig). On PD 34, animals were tested in the elevated plus maze for 5 min and were sacrificed for brain tissue collection. Pyknotic cells were stained with toluidine blue and quantified in the central amygdala (CeA). EtOH-treated animals showed an anxiogenic profile, spending less time in open arms; a profile that was improved by ω-3 (EtOH+ω-3 animals increase the time in open arms). EtOH significantly increased the pyknotic cell number in the CeA. These findings highlight ethanol´s long-term neurotoxic effects on cell degeneration and anxiety and provide evidence of possible amelioration of ω-3 in adolescents.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Encuentro
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/290721
Binge-like ethanol induced anxiety- like behavior, cell degeneration and possible amelioration of Omega-3 (ω-3) Fatty Acids in adolescent rats; XL SAN Meeting; Buenos Aires; Argentina; 2025; 67-67
CONICET Digital
CONICET
url http://hdl.handle.net/11336/290721
identifier_str_mv Binge-like ethanol induced anxiety- like behavior, cell degeneration and possible amelioration of Omega-3 (ω-3) Fatty Acids in adolescent rats; XL SAN Meeting; Buenos Aires; Argentina; 2025; 67-67
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://csan2025.saneurociencias.org.ar/poster/binge-like-ethanol-induced-anxiety-like-behavior-cell-degeneration-and-possible-amelioration-of-omega-3-%CF%89-3-fatty-acids-in-adolescent-rats/
info:eu-repo/semantics/altIdentifier/url/https://csan2025.saneurociencias.org.ar/wp-content/uploads/2025/10/SAN2025-Posters-EBOOK-Final-1.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
publisher.none.fl_str_mv Sociedad Argentina de Investigación en Neurociencias
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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