Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells

Autores
Schaaf, Christian; Shan, Bing; Onofri, Chiara; Stalla, Günter K.; Arzt, Eduardo Simon; Schilling, Tobias; Perone, Marcelo Javier; Renner, Ulrich
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The polyphenol curcumin (diferuloylmethane) is the active componenet of the spice plant Curcuma longa and has been shown to exert multiple actions on mammalian cells. We have studied its effect on folliculostellate (FS) TtT/GF mouse pituitary cells, representative of a multifunctional, endocrine inactive cell type of the anterior pituitary. Proliferation of TtT/GF cells was inhibited by curcumin in a monolayer cell culture and in the colony formation assay in soft agar. Fluorescence-activated cell-sorting (FACS) analysis demonstrated curcumin-induced cell cycle arrest at G2/M accompanied by inhibition of cyclin D1 protein expression. Curcumin had a small effect on necrosis of TtT/GF cells, but it mainly stimulated apoptosis as demonstrated by FACS analysis (Annexin V-fluorescein isothiocyannate/7-aminoactinomycin D staining). Curcumin-induced apoptosis involved suppression of Bcl-2, stimulation of cleaved caspase-3 and induction of DNA fragmentation. Functional studies on FS cell-derived compounds showed that curcumin inhibited mRNA synthesis and release of angiogenic vascular endothelial growth factor-A (VEGF-A). Immune-like functions of FS cells were impaired since curcumin downregulated Toll-like receptor 4, reduced nuclear factor-κB expression and suppressed bacterial endotoxin-induced interleukin-6 (IL-6) secretion. The inhibitory action of curcumin on VEGF-A and IL-6 production was also found in primary rat pituitary cell cultures, in which FS cells are the only source of these proteins. The observed effects of curcumin on FS cell growth, apoptosis and functions may have therapeutic consequences for the intrapituitary regulation of hormone production and release as well as for pituitary tumor pathogenesis.
Fil: Schaaf, Christian. University of Heidelberg; Alemania. Max Planck Institute of Psychiatry; Alemania
Fil: Shan, Bing. Max Planck Institute of Psychiatry; Alemania
Fil: Onofri, Chiara. Max Planck Institute of Psychiatry; Alemania
Fil: Stalla, Günter K.. Max Planck Institute of Psychiatry; Alemania
Fil: Arzt, Eduardo Simon. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Schilling, Tobias. University of Heidelberg; Alemania
Fil: Perone, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Renner, Ulrich. Max Planck Institute of Psychiatry; Alemania
Materia
Apoptosis
Cell Cycle
Curcumin
Folliculostellate Pituitary Cells
Interleukin-6
Nuclear Factor-ΚB
Proliferation
Tlr4
Vascular Endothelial Growth Factor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/68918
Acceder
id CONICETDig_ff38cefb2d434967184859aa6d6f5377
oai_identifier_str oai:ri.conicet.gov.ar:11336/68918
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cellsSchaaf, ChristianShan, BingOnofri, ChiaraStalla, Günter K.Arzt, Eduardo SimonSchilling, TobiasPerone, Marcelo JavierRenner, UlrichApoptosisCell CycleCurcuminFolliculostellate Pituitary CellsInterleukin-6Nuclear Factor-ΚBProliferationTlr4Vascular Endothelial Growth Factorhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The polyphenol curcumin (diferuloylmethane) is the active componenet of the spice plant Curcuma longa and has been shown to exert multiple actions on mammalian cells. We have studied its effect on folliculostellate (FS) TtT/GF mouse pituitary cells, representative of a multifunctional, endocrine inactive cell type of the anterior pituitary. Proliferation of TtT/GF cells was inhibited by curcumin in a monolayer cell culture and in the colony formation assay in soft agar. Fluorescence-activated cell-sorting (FACS) analysis demonstrated curcumin-induced cell cycle arrest at G2/M accompanied by inhibition of cyclin D1 protein expression. Curcumin had a small effect on necrosis of TtT/GF cells, but it mainly stimulated apoptosis as demonstrated by FACS analysis (Annexin V-fluorescein isothiocyannate/7-aminoactinomycin D staining). Curcumin-induced apoptosis involved suppression of Bcl-2, stimulation of cleaved caspase-3 and induction of DNA fragmentation. Functional studies on FS cell-derived compounds showed that curcumin inhibited mRNA synthesis and release of angiogenic vascular endothelial growth factor-A (VEGF-A). Immune-like functions of FS cells were impaired since curcumin downregulated Toll-like receptor 4, reduced nuclear factor-κB expression and suppressed bacterial endotoxin-induced interleukin-6 (IL-6) secretion. The inhibitory action of curcumin on VEGF-A and IL-6 production was also found in primary rat pituitary cell cultures, in which FS cells are the only source of these proteins. The observed effects of curcumin on FS cell growth, apoptosis and functions may have therapeutic consequences for the intrapituitary regulation of hormone production and release as well as for pituitary tumor pathogenesis.Fil: Schaaf, Christian. University of Heidelberg; Alemania. Max Planck Institute of Psychiatry; AlemaniaFil: Shan, Bing. Max Planck Institute of Psychiatry; AlemaniaFil: Onofri, Chiara. Max Planck Institute of Psychiatry; AlemaniaFil: Stalla, Günter K.. Max Planck Institute of Psychiatry; AlemaniaFil: Arzt, Eduardo Simon. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Schilling, Tobias. University of Heidelberg; AlemaniaFil: Perone, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Renner, Ulrich. Max Planck Institute of Psychiatry; AlemaniaKarger2010-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/68918Schaaf, Christian; Shan, Bing; Onofri, Chiara; Stalla, Günter K.; Arzt, Eduardo Simon; et al.; Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells; Karger; Neuroendocrinology; 91; 2; 3-2010; 200-2100028-3835CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1159/000287236info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/287236info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T14:42:56Zoai:ri.conicet.gov.ar:11336/68918instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 14:42:56.603CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells
title Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells
spellingShingle Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells
Schaaf, Christian
Apoptosis
Cell Cycle
Curcumin
Folliculostellate Pituitary Cells
Interleukin-6
Nuclear Factor-ΚB
Proliferation
Tlr4
Vascular Endothelial Growth Factor
title_short Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells
title_full Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells
title_fullStr Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells
title_full_unstemmed Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells
title_sort Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells
dc.creator.none.fl_str_mv Schaaf, Christian
Shan, Bing
Onofri, Chiara
Stalla, Günter K.
Arzt, Eduardo Simon
Schilling, Tobias
Perone, Marcelo Javier
Renner, Ulrich
author Schaaf, Christian
author_facet Schaaf, Christian
Shan, Bing
Onofri, Chiara
Stalla, Günter K.
Arzt, Eduardo Simon
Schilling, Tobias
Perone, Marcelo Javier
Renner, Ulrich
author_role author
author2 Shan, Bing
Onofri, Chiara
Stalla, Günter K.
Arzt, Eduardo Simon
Schilling, Tobias
Perone, Marcelo Javier
Renner, Ulrich
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Apoptosis
Cell Cycle
Curcumin
Folliculostellate Pituitary Cells
Interleukin-6
Nuclear Factor-ΚB
Proliferation
Tlr4
Vascular Endothelial Growth Factor
topic Apoptosis
Cell Cycle
Curcumin
Folliculostellate Pituitary Cells
Interleukin-6
Nuclear Factor-ΚB
Proliferation
Tlr4
Vascular Endothelial Growth Factor
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The polyphenol curcumin (diferuloylmethane) is the active componenet of the spice plant Curcuma longa and has been shown to exert multiple actions on mammalian cells. We have studied its effect on folliculostellate (FS) TtT/GF mouse pituitary cells, representative of a multifunctional, endocrine inactive cell type of the anterior pituitary. Proliferation of TtT/GF cells was inhibited by curcumin in a monolayer cell culture and in the colony formation assay in soft agar. Fluorescence-activated cell-sorting (FACS) analysis demonstrated curcumin-induced cell cycle arrest at G2/M accompanied by inhibition of cyclin D1 protein expression. Curcumin had a small effect on necrosis of TtT/GF cells, but it mainly stimulated apoptosis as demonstrated by FACS analysis (Annexin V-fluorescein isothiocyannate/7-aminoactinomycin D staining). Curcumin-induced apoptosis involved suppression of Bcl-2, stimulation of cleaved caspase-3 and induction of DNA fragmentation. Functional studies on FS cell-derived compounds showed that curcumin inhibited mRNA synthesis and release of angiogenic vascular endothelial growth factor-A (VEGF-A). Immune-like functions of FS cells were impaired since curcumin downregulated Toll-like receptor 4, reduced nuclear factor-κB expression and suppressed bacterial endotoxin-induced interleukin-6 (IL-6) secretion. The inhibitory action of curcumin on VEGF-A and IL-6 production was also found in primary rat pituitary cell cultures, in which FS cells are the only source of these proteins. The observed effects of curcumin on FS cell growth, apoptosis and functions may have therapeutic consequences for the intrapituitary regulation of hormone production and release as well as for pituitary tumor pathogenesis.
Fil: Schaaf, Christian. University of Heidelberg; Alemania. Max Planck Institute of Psychiatry; Alemania
Fil: Shan, Bing. Max Planck Institute of Psychiatry; Alemania
Fil: Onofri, Chiara. Max Planck Institute of Psychiatry; Alemania
Fil: Stalla, Günter K.. Max Planck Institute of Psychiatry; Alemania
Fil: Arzt, Eduardo Simon. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Schilling, Tobias. University of Heidelberg; Alemania
Fil: Perone, Marcelo Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Renner, Ulrich. Max Planck Institute of Psychiatry; Alemania
description The polyphenol curcumin (diferuloylmethane) is the active componenet of the spice plant Curcuma longa and has been shown to exert multiple actions on mammalian cells. We have studied its effect on folliculostellate (FS) TtT/GF mouse pituitary cells, representative of a multifunctional, endocrine inactive cell type of the anterior pituitary. Proliferation of TtT/GF cells was inhibited by curcumin in a monolayer cell culture and in the colony formation assay in soft agar. Fluorescence-activated cell-sorting (FACS) analysis demonstrated curcumin-induced cell cycle arrest at G2/M accompanied by inhibition of cyclin D1 protein expression. Curcumin had a small effect on necrosis of TtT/GF cells, but it mainly stimulated apoptosis as demonstrated by FACS analysis (Annexin V-fluorescein isothiocyannate/7-aminoactinomycin D staining). Curcumin-induced apoptosis involved suppression of Bcl-2, stimulation of cleaved caspase-3 and induction of DNA fragmentation. Functional studies on FS cell-derived compounds showed that curcumin inhibited mRNA synthesis and release of angiogenic vascular endothelial growth factor-A (VEGF-A). Immune-like functions of FS cells were impaired since curcumin downregulated Toll-like receptor 4, reduced nuclear factor-κB expression and suppressed bacterial endotoxin-induced interleukin-6 (IL-6) secretion. The inhibitory action of curcumin on VEGF-A and IL-6 production was also found in primary rat pituitary cell cultures, in which FS cells are the only source of these proteins. The observed effects of curcumin on FS cell growth, apoptosis and functions may have therapeutic consequences for the intrapituitary regulation of hormone production and release as well as for pituitary tumor pathogenesis.
publishDate 2010
dc.date.none.fl_str_mv 2010-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/68918
Schaaf, Christian; Shan, Bing; Onofri, Chiara; Stalla, Günter K.; Arzt, Eduardo Simon; et al.; Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells; Karger; Neuroendocrinology; 91; 2; 3-2010; 200-210
0028-3835
CONICET Digital
CONICET
url http://hdl.handle.net/11336/68918
identifier_str_mv Schaaf, Christian; Shan, Bing; Onofri, Chiara; Stalla, Günter K.; Arzt, Eduardo Simon; et al.; Curcumin inhibits the growth, induces apoptosis and modulates the function of pituitary folliculostellate cells; Karger; Neuroendocrinology; 91; 2; 3-2010; 200-210
0028-3835
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1159/000287236
info:eu-repo/semantics/altIdentifier/url/https://www.karger.com/Article/Abstract/287236
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1874774531285975040
score 13.265058

Publicaciones similares