Genomic and Proteomic Insights into Arsenic Detoxification and Alternative Transformation Pathways in Microbacterium oxydans AE038-20

Autores
Spuches, Florencia Cecilia; Morales, Andrés Hernán; Hero, Johan Sebastian; Pisa, José Horacio; Galván, Adriana Emilce; Ferrero, Marcela Alejandra; Romero, Cintia Mariana
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Arsenic-contaminated groundwater is a major environmental concern, particularly in northern Argentina. Here, Microbacterium oxydans AE038-20, isolated from arsenic-rich groundwater, was investigated to elucidate its tolerance and transformation capacity. Growth assays showed that the strain tolerates inorganic arsenic [As(III), As(V)] and methylarsenite [MAs(III)] without significant inhibition. Speciation analyses revealed progressive oxidation of As(III) to As(V), reaching near-complete conversion after 10 days. Similarly, MAs(III) was fully oxidized to MAs(V). Genome sequencing identified ars-related determinants, including arsR, arsC, putative arsenite efflux systems, and arsP, supporting detoxification via arsenate reduction and arsenite efflux. Proteomic analyses confirmed the expression of proteins related to arsenic resistance, oxidative stress response, and metal transport. However, no canonical arsenite oxidases were detected at either the genomic or proteomic level. Despite this, M. oxydans AE038-20 exhibited clear arsenic oxidation activity. The detection of pigment-associated proteins and in vitro oxidation assays suggest an alternative mechanism potentially mediated by redox-active pigments. These findings highlight an alternative pathway for arsenic transformation in environmental bacteria.
Fil: Spuches, Florencia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Morales, Andrés Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Hero, Johan Sebastian. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pisa, José Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Galván, Adriana Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ferrero, Marcela Alejandra. Universidad Nacional Arturo Jauretche. Instituto de Ingeniería y Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romero, Cintia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Materia
arsenic biotransformation
Microbacterium
redox-active pigment
proteomic
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/290369
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/290369
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genomic and Proteomic Insights into Arsenic Detoxification and Alternative Transformation Pathways in Microbacterium oxydans AE038-20Spuches, Florencia CeciliaMorales, Andrés HernánHero, Johan SebastianPisa, José HoracioGalván, Adriana EmilceFerrero, Marcela AlejandraRomero, Cintia Marianaarsenic biotransformationMicrobacteriumredox-active pigmentproteomichttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Arsenic-contaminated groundwater is a major environmental concern, particularly in northern Argentina. Here, Microbacterium oxydans AE038-20, isolated from arsenic-rich groundwater, was investigated to elucidate its tolerance and transformation capacity. Growth assays showed that the strain tolerates inorganic arsenic [As(III), As(V)] and methylarsenite [MAs(III)] without significant inhibition. Speciation analyses revealed progressive oxidation of As(III) to As(V), reaching near-complete conversion after 10 days. Similarly, MAs(III) was fully oxidized to MAs(V). Genome sequencing identified ars-related determinants, including arsR, arsC, putative arsenite efflux systems, and arsP, supporting detoxification via arsenate reduction and arsenite efflux. Proteomic analyses confirmed the expression of proteins related to arsenic resistance, oxidative stress response, and metal transport. However, no canonical arsenite oxidases were detected at either the genomic or proteomic level. Despite this, M. oxydans AE038-20 exhibited clear arsenic oxidation activity. The detection of pigment-associated proteins and in vitro oxidation assays suggest an alternative mechanism potentially mediated by redox-active pigments. These findings highlight an alternative pathway for arsenic transformation in environmental bacteria.Fil: Spuches, Florencia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Morales, Andrés Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Hero, Johan Sebastian. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pisa, José Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Galván, Adriana Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Ferrero, Marcela Alejandra. Universidad Nacional Arturo Jauretche. Instituto de Ingeniería y Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romero, Cintia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaMDPI2026-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/290369Spuches, Florencia Cecilia; Morales, Andrés Hernán; Hero, Johan Sebastian; Pisa, José Horacio; Galván, Adriana Emilce; et al.; Genomic and Proteomic Insights into Arsenic Detoxification and Alternative Transformation Pathways in Microbacterium oxydans AE038-20; MDPI; Processes; 14; 9; 4-2026; 1-162227-9717CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2227-9717/14/9/1395info:eu-repo/semantics/altIdentifier/doi/10.3390/pr14091395info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-08-25T15:49:28Zoai:ri.conicet.gov.ar:11336/290369instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-08-25 15:49:28.734CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genomic and Proteomic Insights into Arsenic Detoxification and Alternative Transformation Pathways in Microbacterium oxydans AE038-20
title Genomic and Proteomic Insights into Arsenic Detoxification and Alternative Transformation Pathways in Microbacterium oxydans AE038-20
spellingShingle Genomic and Proteomic Insights into Arsenic Detoxification and Alternative Transformation Pathways in Microbacterium oxydans AE038-20
Spuches, Florencia Cecilia
arsenic biotransformation
Microbacterium
redox-active pigment
proteomic
title_short Genomic and Proteomic Insights into Arsenic Detoxification and Alternative Transformation Pathways in Microbacterium oxydans AE038-20
title_full Genomic and Proteomic Insights into Arsenic Detoxification and Alternative Transformation Pathways in Microbacterium oxydans AE038-20
title_fullStr Genomic and Proteomic Insights into Arsenic Detoxification and Alternative Transformation Pathways in Microbacterium oxydans AE038-20
title_full_unstemmed Genomic and Proteomic Insights into Arsenic Detoxification and Alternative Transformation Pathways in Microbacterium oxydans AE038-20
title_sort Genomic and Proteomic Insights into Arsenic Detoxification and Alternative Transformation Pathways in Microbacterium oxydans AE038-20
dc.creator.none.fl_str_mv Spuches, Florencia Cecilia
Morales, Andrés Hernán
Hero, Johan Sebastian
Pisa, José Horacio
Galván, Adriana Emilce
Ferrero, Marcela Alejandra
Romero, Cintia Mariana
author Spuches, Florencia Cecilia
author_facet Spuches, Florencia Cecilia
Morales, Andrés Hernán
Hero, Johan Sebastian
Pisa, José Horacio
Galván, Adriana Emilce
Ferrero, Marcela Alejandra
Romero, Cintia Mariana
author_role author
author2 Morales, Andrés Hernán
Hero, Johan Sebastian
Pisa, José Horacio
Galván, Adriana Emilce
Ferrero, Marcela Alejandra
Romero, Cintia Mariana
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv arsenic biotransformation
Microbacterium
redox-active pigment
proteomic
topic arsenic biotransformation
Microbacterium
redox-active pigment
proteomic
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Arsenic-contaminated groundwater is a major environmental concern, particularly in northern Argentina. Here, Microbacterium oxydans AE038-20, isolated from arsenic-rich groundwater, was investigated to elucidate its tolerance and transformation capacity. Growth assays showed that the strain tolerates inorganic arsenic [As(III), As(V)] and methylarsenite [MAs(III)] without significant inhibition. Speciation analyses revealed progressive oxidation of As(III) to As(V), reaching near-complete conversion after 10 days. Similarly, MAs(III) was fully oxidized to MAs(V). Genome sequencing identified ars-related determinants, including arsR, arsC, putative arsenite efflux systems, and arsP, supporting detoxification via arsenate reduction and arsenite efflux. Proteomic analyses confirmed the expression of proteins related to arsenic resistance, oxidative stress response, and metal transport. However, no canonical arsenite oxidases were detected at either the genomic or proteomic level. Despite this, M. oxydans AE038-20 exhibited clear arsenic oxidation activity. The detection of pigment-associated proteins and in vitro oxidation assays suggest an alternative mechanism potentially mediated by redox-active pigments. These findings highlight an alternative pathway for arsenic transformation in environmental bacteria.
Fil: Spuches, Florencia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Morales, Andrés Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Hero, Johan Sebastian. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pisa, José Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Galván, Adriana Emilce. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Ferrero, Marcela Alejandra. Universidad Nacional Arturo Jauretche. Instituto de Ingeniería y Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romero, Cintia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
description Arsenic-contaminated groundwater is a major environmental concern, particularly in northern Argentina. Here, Microbacterium oxydans AE038-20, isolated from arsenic-rich groundwater, was investigated to elucidate its tolerance and transformation capacity. Growth assays showed that the strain tolerates inorganic arsenic [As(III), As(V)] and methylarsenite [MAs(III)] without significant inhibition. Speciation analyses revealed progressive oxidation of As(III) to As(V), reaching near-complete conversion after 10 days. Similarly, MAs(III) was fully oxidized to MAs(V). Genome sequencing identified ars-related determinants, including arsR, arsC, putative arsenite efflux systems, and arsP, supporting detoxification via arsenate reduction and arsenite efflux. Proteomic analyses confirmed the expression of proteins related to arsenic resistance, oxidative stress response, and metal transport. However, no canonical arsenite oxidases were detected at either the genomic or proteomic level. Despite this, M. oxydans AE038-20 exhibited clear arsenic oxidation activity. The detection of pigment-associated proteins and in vitro oxidation assays suggest an alternative mechanism potentially mediated by redox-active pigments. These findings highlight an alternative pathway for arsenic transformation in environmental bacteria.
publishDate 2026
dc.date.none.fl_str_mv 2026-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/290369
Spuches, Florencia Cecilia; Morales, Andrés Hernán; Hero, Johan Sebastian; Pisa, José Horacio; Galván, Adriana Emilce; et al.; Genomic and Proteomic Insights into Arsenic Detoxification and Alternative Transformation Pathways in Microbacterium oxydans AE038-20; MDPI; Processes; 14; 9; 4-2026; 1-16
2227-9717
CONICET Digital
CONICET
url http://hdl.handle.net/11336/290369
identifier_str_mv Spuches, Florencia Cecilia; Morales, Andrés Hernán; Hero, Johan Sebastian; Pisa, José Horacio; Galván, Adriana Emilce; et al.; Genomic and Proteomic Insights into Arsenic Detoxification and Alternative Transformation Pathways in Microbacterium oxydans AE038-20; MDPI; Processes; 14; 9; 4-2026; 1-16
2227-9717
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2227-9717/14/9/1395
info:eu-repo/semantics/altIdentifier/doi/10.3390/pr14091395
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.24418

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