Cytokine gene transcription in the trachea, Harderian gland, and trigeminal ganglia of chickens inoculated with virulent infectious laryngotracheitis virus (ILTV) strain

Autores
Vagnozzi, Ariel Eduardo; Beltrán, Gabriela; Zavala, Guillermo; Read, Leah; Sharif, Shayan; García, Maricarmen
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of this study was to determine how cytokine transcription profiles correlate with patterns of infectious laryngotracheitis virus (ILTV) replication in the trachea, Harderian gland, and trigeminal ganglia during the early and late stages of infection after intratracheal inoculation. Viral genomes and transcripts were detected in the trachea and Harderian gland but not in trigeminal ganglia. The onset of viral replication in the trachea was detected at day one post-infection and peaked by day three post-infection. The peak of proinflammatory (CXCLi2, IL-1β, IFN-γ) and anti-inflammatory (IL-13, IL-10) cytokine gene transcription, 5 days post-infection, coincided with the increased recruitment of inflammatory cells, extensive tissue damage, and limiting of virus replication in the trachea. In contrast, transcription of the IFN-β gene in the trachea remained unaffected suggesting that ILTV infection blocks type I interferon responses. In the Harderian gland, the most evident transcription change was the early and transient upregulation of the IFN-γ gene at 1 day post-infection, which suggests that the Harderian gland is prepared to rapidly respond to ILTV infection. Overall, results from this study suggest that regulation of Th1 effector cells and macrophage activity by Th1/2 cytokines was pertinent to maintain a balanced immune response capable of providing an adequate Th1-mediated protective immunity, while sustaining some immune homeostasis in preparation for the regeneration of the tracheal mucosa.
Instituto de Virología
Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Beltrán, Gabriela. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos
Fil: Zavala, Guillermo. Avian Health International; Estados Unidos
Fil: Read, Leah. University of Guelph. Ontario Veterinary College. Department of Pathobiology; Canadá
Fil: Sharif, Shayan. University of Guelph. Ontario Veterinary College. Department of Pathobiology; Canadá
Fil: García, Maricarmen. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos
Fuente
Avian Pathology 47 (5) : 497-508. (October 2018)
Materia
Citoquina
Transcripción Génica
Tráquea
Pollo
Laringotraqueitis Infecciosa Aviar
Inoculación
Cytokines
Gene Transcription
Trachea
Chickens
Avian Infectious Laryngotracheitis
Inoculation
Harderian Gland
Trigeminal Ganglia
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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oai_identifier_str oai:localhost:20.500.12123/27692
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spelling Cytokine gene transcription in the trachea, Harderian gland, and trigeminal ganglia of chickens inoculated with virulent infectious laryngotracheitis virus (ILTV) strainVagnozzi, Ariel EduardoBeltrán, GabrielaZavala, GuillermoRead, LeahSharif, ShayanGarcía, MaricarmenCitoquinaTranscripción GénicaTráqueaPolloLaringotraqueitis Infecciosa AviarInoculaciónCytokinesGene TranscriptionTracheaChickensAvian Infectious LaryngotracheitisInoculationHarderian GlandTrigeminal GangliaThe objective of this study was to determine how cytokine transcription profiles correlate with patterns of infectious laryngotracheitis virus (ILTV) replication in the trachea, Harderian gland, and trigeminal ganglia during the early and late stages of infection after intratracheal inoculation. Viral genomes and transcripts were detected in the trachea and Harderian gland but not in trigeminal ganglia. The onset of viral replication in the trachea was detected at day one post-infection and peaked by day three post-infection. The peak of proinflammatory (CXCLi2, IL-1β, IFN-γ) and anti-inflammatory (IL-13, IL-10) cytokine gene transcription, 5 days post-infection, coincided with the increased recruitment of inflammatory cells, extensive tissue damage, and limiting of virus replication in the trachea. In contrast, transcription of the IFN-β gene in the trachea remained unaffected suggesting that ILTV infection blocks type I interferon responses. In the Harderian gland, the most evident transcription change was the early and transient upregulation of the IFN-γ gene at 1 day post-infection, which suggests that the Harderian gland is prepared to rapidly respond to ILTV infection. Overall, results from this study suggest that regulation of Th1 effector cells and macrophage activity by Th1/2 cytokines was pertinent to maintain a balanced immune response capable of providing an adequate Th1-mediated protective immunity, while sustaining some immune homeostasis in preparation for the regeneration of the tracheal mucosa.Instituto de VirologíaFil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Beltrán, Gabriela. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados UnidosFil: Zavala, Guillermo. Avian Health International; Estados UnidosFil: Read, Leah. University of Guelph. Ontario Veterinary College. Department of Pathobiology; CanadáFil: Sharif, Shayan. University of Guelph. Ontario Veterinary College. Department of Pathobiology; CanadáFil: García, Maricarmen. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados UnidosTaylor and Francis2026-09-08T14:09:11Z2026-09-08T14:09:11Z2018-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/27692https://www.tandfonline.com/doi/full/10.1080/03079457.2018.14920900307-9457 (impreso)1465-3338 (online)https://doi.org/10.1080/03079457.2018.1492090Avian Pathology 47 (5) : 497-508. (October 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:47Zoai:localhost:20.500.12123/27692instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:48.549INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Cytokine gene transcription in the trachea, Harderian gland, and trigeminal ganglia of chickens inoculated with virulent infectious laryngotracheitis virus (ILTV) strain
title Cytokine gene transcription in the trachea, Harderian gland, and trigeminal ganglia of chickens inoculated with virulent infectious laryngotracheitis virus (ILTV) strain
spellingShingle Cytokine gene transcription in the trachea, Harderian gland, and trigeminal ganglia of chickens inoculated with virulent infectious laryngotracheitis virus (ILTV) strain
Vagnozzi, Ariel Eduardo
Citoquina
Transcripción Génica
Tráquea
Pollo
Laringotraqueitis Infecciosa Aviar
Inoculación
Cytokines
Gene Transcription
Trachea
Chickens
Avian Infectious Laryngotracheitis
Inoculation
Harderian Gland
Trigeminal Ganglia
title_short Cytokine gene transcription in the trachea, Harderian gland, and trigeminal ganglia of chickens inoculated with virulent infectious laryngotracheitis virus (ILTV) strain
title_full Cytokine gene transcription in the trachea, Harderian gland, and trigeminal ganglia of chickens inoculated with virulent infectious laryngotracheitis virus (ILTV) strain
title_fullStr Cytokine gene transcription in the trachea, Harderian gland, and trigeminal ganglia of chickens inoculated with virulent infectious laryngotracheitis virus (ILTV) strain
title_full_unstemmed Cytokine gene transcription in the trachea, Harderian gland, and trigeminal ganglia of chickens inoculated with virulent infectious laryngotracheitis virus (ILTV) strain
title_sort Cytokine gene transcription in the trachea, Harderian gland, and trigeminal ganglia of chickens inoculated with virulent infectious laryngotracheitis virus (ILTV) strain
dc.creator.none.fl_str_mv Vagnozzi, Ariel Eduardo
Beltrán, Gabriela
Zavala, Guillermo
Read, Leah
Sharif, Shayan
García, Maricarmen
author Vagnozzi, Ariel Eduardo
author_facet Vagnozzi, Ariel Eduardo
Beltrán, Gabriela
Zavala, Guillermo
Read, Leah
Sharif, Shayan
García, Maricarmen
author_role author
author2 Beltrán, Gabriela
Zavala, Guillermo
Read, Leah
Sharif, Shayan
García, Maricarmen
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Citoquina
Transcripción Génica
Tráquea
Pollo
Laringotraqueitis Infecciosa Aviar
Inoculación
Cytokines
Gene Transcription
Trachea
Chickens
Avian Infectious Laryngotracheitis
Inoculation
Harderian Gland
Trigeminal Ganglia
topic Citoquina
Transcripción Génica
Tráquea
Pollo
Laringotraqueitis Infecciosa Aviar
Inoculación
Cytokines
Gene Transcription
Trachea
Chickens
Avian Infectious Laryngotracheitis
Inoculation
Harderian Gland
Trigeminal Ganglia
dc.description.none.fl_txt_mv The objective of this study was to determine how cytokine transcription profiles correlate with patterns of infectious laryngotracheitis virus (ILTV) replication in the trachea, Harderian gland, and trigeminal ganglia during the early and late stages of infection after intratracheal inoculation. Viral genomes and transcripts were detected in the trachea and Harderian gland but not in trigeminal ganglia. The onset of viral replication in the trachea was detected at day one post-infection and peaked by day three post-infection. The peak of proinflammatory (CXCLi2, IL-1β, IFN-γ) and anti-inflammatory (IL-13, IL-10) cytokine gene transcription, 5 days post-infection, coincided with the increased recruitment of inflammatory cells, extensive tissue damage, and limiting of virus replication in the trachea. In contrast, transcription of the IFN-β gene in the trachea remained unaffected suggesting that ILTV infection blocks type I interferon responses. In the Harderian gland, the most evident transcription change was the early and transient upregulation of the IFN-γ gene at 1 day post-infection, which suggests that the Harderian gland is prepared to rapidly respond to ILTV infection. Overall, results from this study suggest that regulation of Th1 effector cells and macrophage activity by Th1/2 cytokines was pertinent to maintain a balanced immune response capable of providing an adequate Th1-mediated protective immunity, while sustaining some immune homeostasis in preparation for the regeneration of the tracheal mucosa.
Instituto de Virología
Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Beltrán, Gabriela. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos
Fil: Zavala, Guillermo. Avian Health International; Estados Unidos
Fil: Read, Leah. University of Guelph. Ontario Veterinary College. Department of Pathobiology; Canadá
Fil: Sharif, Shayan. University of Guelph. Ontario Veterinary College. Department of Pathobiology; Canadá
Fil: García, Maricarmen. University of Georgia. College of Veterinary Medicine. Poultry Diagnostic and Research Center; Estados Unidos
description The objective of this study was to determine how cytokine transcription profiles correlate with patterns of infectious laryngotracheitis virus (ILTV) replication in the trachea, Harderian gland, and trigeminal ganglia during the early and late stages of infection after intratracheal inoculation. Viral genomes and transcripts were detected in the trachea and Harderian gland but not in trigeminal ganglia. The onset of viral replication in the trachea was detected at day one post-infection and peaked by day three post-infection. The peak of proinflammatory (CXCLi2, IL-1β, IFN-γ) and anti-inflammatory (IL-13, IL-10) cytokine gene transcription, 5 days post-infection, coincided with the increased recruitment of inflammatory cells, extensive tissue damage, and limiting of virus replication in the trachea. In contrast, transcription of the IFN-β gene in the trachea remained unaffected suggesting that ILTV infection blocks type I interferon responses. In the Harderian gland, the most evident transcription change was the early and transient upregulation of the IFN-γ gene at 1 day post-infection, which suggests that the Harderian gland is prepared to rapidly respond to ILTV infection. Overall, results from this study suggest that regulation of Th1 effector cells and macrophage activity by Th1/2 cytokines was pertinent to maintain a balanced immune response capable of providing an adequate Th1-mediated protective immunity, while sustaining some immune homeostasis in preparation for the regeneration of the tracheal mucosa.
publishDate 2018
dc.date.none.fl_str_mv 2018-10
2026-09-08T14:09:11Z
2026-09-08T14:09:11Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/27692
https://www.tandfonline.com/doi/full/10.1080/03079457.2018.1492090
0307-9457 (impreso)
1465-3338 (online)
https://doi.org/10.1080/03079457.2018.1492090
url http://hdl.handle.net/20.500.12123/27692
https://www.tandfonline.com/doi/full/10.1080/03079457.2018.1492090
https://doi.org/10.1080/03079457.2018.1492090
identifier_str_mv 0307-9457 (impreso)
1465-3338 (online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor and Francis
publisher.none.fl_str_mv Taylor and Francis
dc.source.none.fl_str_mv Avian Pathology 47 (5) : 497-508. (October 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.754232

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