Transcriptome derived microsatellite markers in Valeriana clarionifolia reveal transferability among Patagonian Valeriana species

Autores
Manifesto, Maria Marcela; Soria, Marcelo Abel; Galvan, Fatima Veronica; Rivero, Alexander Gabriel; Costa Tártara, Sabrina María; Arizio, Carla Marcela; Nagahama, Nicolás; Fortunato, Renée Hersilia
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Several species within the genus Valeriana are globally recognized for their medicinal properties. However, their increasing economic value has led to overharvesting of natural populations, posing a serious threat to their long-term sustainability. Effective strategies for conservation and rational use require accurate species identification and robust assessment of genetic diversity, yet molecular tools for these purposes remain scarce in genus Valeriana. In this study, we developed microsatellite markers based on transcriptomic data from Valeriana clarionifolia. A total of 2216 putative simple sequence repeat (SSR) loci were identified from a transcriptome deposited in the NCBI Sequence Read Archive (BioProject PRJNA950387), and primers were designed for 691 candidate markers. From 60 selected loci, twelve polymorphic SSR markers were isolated and evaluated in V. carnosa Sm., V. clarionifolia Phil., V. gaimanensis N. Nagh., V. moyanoi Speg., and V. philippiana Briq. Mean cross-species transferability and polymorphism were 91.67% and 83.34%, respectively. The number of alleles per locus ranged from four to fourteen, with an average polymorphism information content (PIC) of 0.67. Among the evaluated loci, primer valcla4800 showed the highest discriminatory capacity (PIC = 0.89). This study represents the first genomic characterization of native Valeriana species from Patagonia and demonstrates the high transferability and informativeness of transcriptome-derived SSR markers within the genus. The molecular tools generated here provide valuable resources for species identification, genetic diversity assessment, and selection of plant material, supporting conservation efforts and sustainable use of medicinal plants.
INTA, Instituto de Recursos Biológicos
Fil: Manifesto, María Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Soria, Marcelo Abel. Universidad de Buenos Aires (UBA). Facultad de Agronomía. Departamento de Microbiología; Argentina
Fil: Galván, Fátima Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Rivero, Alexander Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Rivero, Alexander Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Costa Tártara, Sabrina María. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Costa Tártara, Sabrina María. Universidad Nacional de Luján (UNLu). Departamento de Ciencias Básicas y Tecnología; Argentina
Fil: Arizio, Carla Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Nagahama, Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agroforestal Esquel (EEAf Esquel); Argentina
Fil: Nagahama, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Fortunato, Renée Hersilia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Botánica Darwinion; Argentina
Fil: Fortunato, Renée Hersilia. Academia Nacional de Ciencias Exactas, Físicas y Naturales; Argentina
Fuente
Discover Plants (2026) 3:337
Materia
Valeriana
Microsatélites
Transcriptoma
Plantas Medicinales
Variación Intraespecífica
Microsatellites
Transcriptome
Medicinal Plants
Intraspecific Variation
Región Patagónica
Simple Sequence Repeats
SSR
Valeriana clarionifolia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/27464
Acceder
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oai_identifier_str oai:localhost:20.500.12123/27464
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Transcriptome derived microsatellite markers in Valeriana clarionifolia reveal transferability among Patagonian Valeriana speciesManifesto, Maria MarcelaSoria, Marcelo AbelGalvan, Fatima VeronicaRivero, Alexander GabrielCosta Tártara, Sabrina MaríaArizio, Carla MarcelaNagahama, NicolásFortunato, Renée HersiliaValerianaMicrosatélitesTranscriptomaPlantas MedicinalesVariación IntraespecíficaMicrosatellitesTranscriptomeMedicinal PlantsIntraspecific VariationRegión PatagónicaSimple Sequence RepeatsSSRValeriana clarionifoliaSeveral species within the genus Valeriana are globally recognized for their medicinal properties. However, their increasing economic value has led to overharvesting of natural populations, posing a serious threat to their long-term sustainability. Effective strategies for conservation and rational use require accurate species identification and robust assessment of genetic diversity, yet molecular tools for these purposes remain scarce in genus Valeriana. In this study, we developed microsatellite markers based on transcriptomic data from Valeriana clarionifolia. A total of 2216 putative simple sequence repeat (SSR) loci were identified from a transcriptome deposited in the NCBI Sequence Read Archive (BioProject PRJNA950387), and primers were designed for 691 candidate markers. From 60 selected loci, twelve polymorphic SSR markers were isolated and evaluated in V. carnosa Sm., V. clarionifolia Phil., V. gaimanensis N. Nagh., V. moyanoi Speg., and V. philippiana Briq. Mean cross-species transferability and polymorphism were 91.67% and 83.34%, respectively. The number of alleles per locus ranged from four to fourteen, with an average polymorphism information content (PIC) of 0.67. Among the evaluated loci, primer valcla4800 showed the highest discriminatory capacity (PIC = 0.89). This study represents the first genomic characterization of native Valeriana species from Patagonia and demonstrates the high transferability and informativeness of transcriptome-derived SSR markers within the genus. The molecular tools generated here provide valuable resources for species identification, genetic diversity assessment, and selection of plant material, supporting conservation efforts and sustainable use of medicinal plants.INTA, Instituto de Recursos BiológicosFil: Manifesto, María Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Soria, Marcelo Abel. Universidad de Buenos Aires (UBA). Facultad de Agronomía. Departamento de Microbiología; ArgentinaFil: Galván, Fátima Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Rivero, Alexander Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Rivero, Alexander Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Costa Tártara, Sabrina María. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Costa Tártara, Sabrina María. Universidad Nacional de Luján (UNLu). Departamento de Ciencias Básicas y Tecnología; ArgentinaFil: Arizio, Carla Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Nagahama, Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agroforestal Esquel (EEAf Esquel); ArgentinaFil: Nagahama, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Fortunato, Renée Hersilia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Botánica Darwinion; ArgentinaFil: Fortunato, Renée Hersilia. Academia Nacional de Ciencias Exactas, Físicas y Naturales; ArgentinaSpringer Nature2026-08-19T11:58:25Z2026-08-19T11:58:25Z2026-08-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/27464https://link.springer.com/article/10.1007/s44372-026-00817-4Manifesto, M.M., Soria, M.A., Galván, F.V. et al. Transcriptome derived microsatellite markers in Valeriana clarionifolia reveal transferability among Patagonian Valeriana species. Discov. Plants 3, 337 (2026). https://doi.org/10.1007/s44372-026-00817-43005-1207https://doi.org/10.1007/s44372-026-00817-4Discover Plants (2026) 3:337reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNBIO-1131044/AR./Genómica aplicada a estudios de ecología molecular y diversidad genética.info:eu-repograntAgreement/INTA/PNHFA-1106094/AR./Plataformas tecnológicas y comerciales, para aromáticas cultivadas-nativas y medicinalesPatagonia .......... (región general) (Mundo, Sudamérica, Argentina)7016766info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:42Zoai:localhost:20.500.12123/27464instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:43.24INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Transcriptome derived microsatellite markers in Valeriana clarionifolia reveal transferability among Patagonian Valeriana species
title Transcriptome derived microsatellite markers in Valeriana clarionifolia reveal transferability among Patagonian Valeriana species
spellingShingle Transcriptome derived microsatellite markers in Valeriana clarionifolia reveal transferability among Patagonian Valeriana species
Manifesto, Maria Marcela
Valeriana
Microsatélites
Transcriptoma
Plantas Medicinales
Variación Intraespecífica
Microsatellites
Transcriptome
Medicinal Plants
Intraspecific Variation
Región Patagónica
Simple Sequence Repeats
SSR
Valeriana clarionifolia
title_short Transcriptome derived microsatellite markers in Valeriana clarionifolia reveal transferability among Patagonian Valeriana species
title_full Transcriptome derived microsatellite markers in Valeriana clarionifolia reveal transferability among Patagonian Valeriana species
title_fullStr Transcriptome derived microsatellite markers in Valeriana clarionifolia reveal transferability among Patagonian Valeriana species
title_full_unstemmed Transcriptome derived microsatellite markers in Valeriana clarionifolia reveal transferability among Patagonian Valeriana species
title_sort Transcriptome derived microsatellite markers in Valeriana clarionifolia reveal transferability among Patagonian Valeriana species
dc.creator.none.fl_str_mv Manifesto, Maria Marcela
Soria, Marcelo Abel
Galvan, Fatima Veronica
Rivero, Alexander Gabriel
Costa Tártara, Sabrina María
Arizio, Carla Marcela
Nagahama, Nicolás
Fortunato, Renée Hersilia
author Manifesto, Maria Marcela
author_facet Manifesto, Maria Marcela
Soria, Marcelo Abel
Galvan, Fatima Veronica
Rivero, Alexander Gabriel
Costa Tártara, Sabrina María
Arizio, Carla Marcela
Nagahama, Nicolás
Fortunato, Renée Hersilia
author_role author
author2 Soria, Marcelo Abel
Galvan, Fatima Veronica
Rivero, Alexander Gabriel
Costa Tártara, Sabrina María
Arizio, Carla Marcela
Nagahama, Nicolás
Fortunato, Renée Hersilia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Valeriana
Microsatélites
Transcriptoma
Plantas Medicinales
Variación Intraespecífica
Microsatellites
Transcriptome
Medicinal Plants
Intraspecific Variation
Región Patagónica
Simple Sequence Repeats
SSR
Valeriana clarionifolia
topic Valeriana
Microsatélites
Transcriptoma
Plantas Medicinales
Variación Intraespecífica
Microsatellites
Transcriptome
Medicinal Plants
Intraspecific Variation
Región Patagónica
Simple Sequence Repeats
SSR
Valeriana clarionifolia
dc.description.none.fl_txt_mv Several species within the genus Valeriana are globally recognized for their medicinal properties. However, their increasing economic value has led to overharvesting of natural populations, posing a serious threat to their long-term sustainability. Effective strategies for conservation and rational use require accurate species identification and robust assessment of genetic diversity, yet molecular tools for these purposes remain scarce in genus Valeriana. In this study, we developed microsatellite markers based on transcriptomic data from Valeriana clarionifolia. A total of 2216 putative simple sequence repeat (SSR) loci were identified from a transcriptome deposited in the NCBI Sequence Read Archive (BioProject PRJNA950387), and primers were designed for 691 candidate markers. From 60 selected loci, twelve polymorphic SSR markers were isolated and evaluated in V. carnosa Sm., V. clarionifolia Phil., V. gaimanensis N. Nagh., V. moyanoi Speg., and V. philippiana Briq. Mean cross-species transferability and polymorphism were 91.67% and 83.34%, respectively. The number of alleles per locus ranged from four to fourteen, with an average polymorphism information content (PIC) of 0.67. Among the evaluated loci, primer valcla4800 showed the highest discriminatory capacity (PIC = 0.89). This study represents the first genomic characterization of native Valeriana species from Patagonia and demonstrates the high transferability and informativeness of transcriptome-derived SSR markers within the genus. The molecular tools generated here provide valuable resources for species identification, genetic diversity assessment, and selection of plant material, supporting conservation efforts and sustainable use of medicinal plants.
INTA, Instituto de Recursos Biológicos
Fil: Manifesto, María Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Soria, Marcelo Abel. Universidad de Buenos Aires (UBA). Facultad de Agronomía. Departamento de Microbiología; Argentina
Fil: Galván, Fátima Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Rivero, Alexander Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Rivero, Alexander Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Costa Tártara, Sabrina María. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Costa Tártara, Sabrina María. Universidad Nacional de Luján (UNLu). Departamento de Ciencias Básicas y Tecnología; Argentina
Fil: Arizio, Carla Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Nagahama, Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agroforestal Esquel (EEAf Esquel); Argentina
Fil: Nagahama, Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Fortunato, Renée Hersilia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Botánica Darwinion; Argentina
Fil: Fortunato, Renée Hersilia. Academia Nacional de Ciencias Exactas, Físicas y Naturales; Argentina
description Several species within the genus Valeriana are globally recognized for their medicinal properties. However, their increasing economic value has led to overharvesting of natural populations, posing a serious threat to their long-term sustainability. Effective strategies for conservation and rational use require accurate species identification and robust assessment of genetic diversity, yet molecular tools for these purposes remain scarce in genus Valeriana. In this study, we developed microsatellite markers based on transcriptomic data from Valeriana clarionifolia. A total of 2216 putative simple sequence repeat (SSR) loci were identified from a transcriptome deposited in the NCBI Sequence Read Archive (BioProject PRJNA950387), and primers were designed for 691 candidate markers. From 60 selected loci, twelve polymorphic SSR markers were isolated and evaluated in V. carnosa Sm., V. clarionifolia Phil., V. gaimanensis N. Nagh., V. moyanoi Speg., and V. philippiana Briq. Mean cross-species transferability and polymorphism were 91.67% and 83.34%, respectively. The number of alleles per locus ranged from four to fourteen, with an average polymorphism information content (PIC) of 0.67. Among the evaluated loci, primer valcla4800 showed the highest discriminatory capacity (PIC = 0.89). This study represents the first genomic characterization of native Valeriana species from Patagonia and demonstrates the high transferability and informativeness of transcriptome-derived SSR markers within the genus. The molecular tools generated here provide valuable resources for species identification, genetic diversity assessment, and selection of plant material, supporting conservation efforts and sustainable use of medicinal plants.
publishDate 2026
dc.date.none.fl_str_mv 2026-08-19T11:58:25Z
2026-08-19T11:58:25Z
2026-08-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/27464
https://link.springer.com/article/10.1007/s44372-026-00817-4
Manifesto, M.M., Soria, M.A., Galván, F.V. et al. Transcriptome derived microsatellite markers in Valeriana clarionifolia reveal transferability among Patagonian Valeriana species. Discov. Plants 3, 337 (2026). https://doi.org/10.1007/s44372-026-00817-4
3005-1207
https://doi.org/10.1007/s44372-026-00817-4
url http://hdl.handle.net/20.500.12123/27464
https://link.springer.com/article/10.1007/s44372-026-00817-4
https://doi.org/10.1007/s44372-026-00817-4
identifier_str_mv Manifesto, M.M., Soria, M.A., Galván, F.V. et al. Transcriptome derived microsatellite markers in Valeriana clarionifolia reveal transferability among Patagonian Valeriana species. Discov. Plants 3, 337 (2026). https://doi.org/10.1007/s44372-026-00817-4
3005-1207
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNBIO-1131044/AR./Genómica aplicada a estudios de ecología molecular y diversidad genética.
info:eu-repograntAgreement/INTA/PNHFA-1106094/AR./Plataformas tecnológicas y comerciales, para aromáticas cultivadas-nativas y medicinales
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Patagonia .......... (región general) (Mundo, Sudamérica, Argentina)
7016766
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv Discover Plants (2026) 3:337
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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