Broad-spectrum biocontrol of postharvest rot in cold-stored table grapes by psychrotrophic yeasts and grapevine-derived extracts

Autores
Ferreyra, Susana Gisela; Torres Palazzolo, Carolina Andrea; Riveira, Eimi; Cendoya, Eugenia; Kuhn, Yamila Gisele; Fontana, Ariel; Ramirez, Maria Laura; Pavicich, María Agustina; Combina, Mariana; Ponsone, María Lorena
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Under postharvest conditions, table grapes are exposed to multiple fungal pathogens that coexist and interact on the fruit surface. Therefore, effective biocontrol agents should exert antagonistic activity across multiple pathogens (broad-spectrum biocontrol) while operating within a complex host–microbiota–pathogen system. In this context, four psychrotrophic yeast strains, Metschnikowia pulcherrima RCM2 and ULA146, and Aureobasidium pullulans FUL14 and FUL18, were evaluated for their broad-spectrum biocontrol performance in Red Globe table grapes. Grapevine-derived extracts, rich in phenolic compounds, were included as complementary components capable of enhancing antagonistic activity. Yeasts were tested alone and in combination with extracts from canes (5% and 25%) and bunch stems (5%) on cold-stored table grapes. Treatments were evaluated under three scenarios: single-pathogen inoculation with Botrytis cinerea or Penicillium polonicum, pathogen co-inoculation with B. cinerea, P. polonicum and Alternaria alternata, and non-inoculated storage conditions. A quantitative integrative framework was applied to support the selection of broad-spectrum biocontrol treatments. Overall, the yeast strains showed biocontrol activity against all three pathogens. Yeast–extract combinations significantly reduced P. polonicum infection (p < 0.05). RCM2 exhibited the most consistent performance under pathogen co-inoculation and non-inoculated storage conditions, reaching levels comparable to sulfur dioxide (SO2) treatment. These results support the potential of psychrotrophic yeasts and grapevine by-products as eco-friendly tools for broad-spectrum postharvest disease control in table grapes. They also highlight the importance of integrative approaches that account for pathogen co-occurrence and host complexity when selecting biocontrol agents for successful technological development.
EEA Mendoza
Fil: Ferreyra, Susana G. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional Mendoza; Argentina
Fil: Torres-Palazzolo, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
Fil: Riveira, Eimi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional Mendoza; Argentina
Fil: Cendoya, Eugenia. Universidad Nacional de Río Cuarto. Instituto de Investigación en Micología y Micotoxicología (IMICO); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación en Micología y Micotoxicología (IMICO); Argentina
Fil: Kuhn, Yamila G. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Fontana, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Ramirez, María Laura. Universidad Nacional de Río Cuarto. Instituto de Investigación en Micología y Micotoxicología (IMICO); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación en Micología y Micotoxicología (IMICO); Argentina
Fil: Pavicich, María Agustina. Ghent University. Faculty of Pharmaceutical Sciences. Centre of Excellence in Mycotoxicology & Public Health; Bélgica. Ghent University. Faculty of Bioscience Engineering. Laboratory of Applied Mycology and Phenomics; Bélgica
Fil: Combina, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional Mendoza; Argentina
Fil: Ponsone, María Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
Fuente
International Journal of Food Microbiology 459 : 111911. (October 2026)
Materia
Uvas de Mesa
Compuestos Fenólicos
Control Biológico
Levadura
Almacenamiento en Frío
Table Grapes
Phenolic Compounds
Biological Control
Yeasts
Cold Storage
Biocontrol
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 Internation
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/26989
Acceder
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network_name_str INTA Digital (INTA)
spelling Broad-spectrum biocontrol of postharvest rot in cold-stored table grapes by psychrotrophic yeasts and grapevine-derived extractsFerreyra, Susana GiselaTorres Palazzolo, Carolina AndreaRiveira, EimiCendoya, EugeniaKuhn, Yamila GiseleFontana, ArielRamirez, Maria LauraPavicich, María AgustinaCombina, MarianaPonsone, María LorenaUvas de MesaCompuestos FenólicosControl BiológicoLevaduraAlmacenamiento en FríoTable GrapesPhenolic CompoundsBiological ControlYeastsCold StorageBiocontrolUnder postharvest conditions, table grapes are exposed to multiple fungal pathogens that coexist and interact on the fruit surface. Therefore, effective biocontrol agents should exert antagonistic activity across multiple pathogens (broad-spectrum biocontrol) while operating within a complex host–microbiota–pathogen system. In this context, four psychrotrophic yeast strains, Metschnikowia pulcherrima RCM2 and ULA146, and Aureobasidium pullulans FUL14 and FUL18, were evaluated for their broad-spectrum biocontrol performance in Red Globe table grapes. Grapevine-derived extracts, rich in phenolic compounds, were included as complementary components capable of enhancing antagonistic activity. Yeasts were tested alone and in combination with extracts from canes (5% and 25%) and bunch stems (5%) on cold-stored table grapes. Treatments were evaluated under three scenarios: single-pathogen inoculation with Botrytis cinerea or Penicillium polonicum, pathogen co-inoculation with B. cinerea, P. polonicum and Alternaria alternata, and non-inoculated storage conditions. A quantitative integrative framework was applied to support the selection of broad-spectrum biocontrol treatments. Overall, the yeast strains showed biocontrol activity against all three pathogens. Yeast–extract combinations significantly reduced P. polonicum infection (p < 0.05). RCM2 exhibited the most consistent performance under pathogen co-inoculation and non-inoculated storage conditions, reaching levels comparable to sulfur dioxide (SO2) treatment. These results support the potential of psychrotrophic yeasts and grapevine by-products as eco-friendly tools for broad-spectrum postharvest disease control in table grapes. They also highlight the importance of integrative approaches that account for pathogen co-occurrence and host complexity when selecting biocontrol agents for successful technological development.EEA MendozaFil: Ferreyra, Susana G. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional Mendoza; ArgentinaFil: Torres-Palazzolo, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; ArgentinaFil: Riveira, Eimi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional Mendoza; ArgentinaFil: Cendoya, Eugenia. Universidad Nacional de Río Cuarto. Instituto de Investigación en Micología y Micotoxicología (IMICO); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación en Micología y Micotoxicología (IMICO); ArgentinaFil: Kuhn, Yamila G. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Fontana, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Instituto de Biología Agrícola de Mendoza; ArgentinaFil: Ramirez, María Laura. Universidad Nacional de Río Cuarto. Instituto de Investigación en Micología y Micotoxicología (IMICO); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación en Micología y Micotoxicología (IMICO); ArgentinaFil: Pavicich, María Agustina. Ghent University. Faculty of Pharmaceutical Sciences. Centre of Excellence in Mycotoxicology & Public Health; Bélgica. Ghent University. Faculty of Bioscience Engineering. Laboratory of Applied Mycology and Phenomics; BélgicaFil: Combina, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional Mendoza; ArgentinaFil: Ponsone, María Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; ArgentinaElsevier2026-07-13T12:05:38Z2026-07-13T12:05:38Z2026-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/26989https://www.sciencedirect.com/science/article/abs/pii/S01681605260029280168-16051879-3460https://doi.org/10.1016/j.ijfoodmicro.2026.111911International Journal of Food Microbiology 459 : 111911. (October 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PE-L01-I002, Aportes para la innovación y el desarrollo en los territorios a través del fortalecimiento de la viticulturainfo:eu-repo/semantics/restrictedAccessCreative Commons Attribution-NonCommercial-ShareAlike 4.0 Internation2026-09-24T11:43:27Zoai:localhost:20.500.12123/26989instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:28.378INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Broad-spectrum biocontrol of postharvest rot in cold-stored table grapes by psychrotrophic yeasts and grapevine-derived extracts
title Broad-spectrum biocontrol of postharvest rot in cold-stored table grapes by psychrotrophic yeasts and grapevine-derived extracts
spellingShingle Broad-spectrum biocontrol of postharvest rot in cold-stored table grapes by psychrotrophic yeasts and grapevine-derived extracts
Ferreyra, Susana Gisela
Uvas de Mesa
Compuestos Fenólicos
Control Biológico
Levadura
Almacenamiento en Frío
Table Grapes
Phenolic Compounds
Biological Control
Yeasts
Cold Storage
Biocontrol
title_short Broad-spectrum biocontrol of postharvest rot in cold-stored table grapes by psychrotrophic yeasts and grapevine-derived extracts
title_full Broad-spectrum biocontrol of postharvest rot in cold-stored table grapes by psychrotrophic yeasts and grapevine-derived extracts
title_fullStr Broad-spectrum biocontrol of postharvest rot in cold-stored table grapes by psychrotrophic yeasts and grapevine-derived extracts
title_full_unstemmed Broad-spectrum biocontrol of postharvest rot in cold-stored table grapes by psychrotrophic yeasts and grapevine-derived extracts
title_sort Broad-spectrum biocontrol of postharvest rot in cold-stored table grapes by psychrotrophic yeasts and grapevine-derived extracts
dc.creator.none.fl_str_mv Ferreyra, Susana Gisela
Torres Palazzolo, Carolina Andrea
Riveira, Eimi
Cendoya, Eugenia
Kuhn, Yamila Gisele
Fontana, Ariel
Ramirez, Maria Laura
Pavicich, María Agustina
Combina, Mariana
Ponsone, María Lorena
author Ferreyra, Susana Gisela
author_facet Ferreyra, Susana Gisela
Torres Palazzolo, Carolina Andrea
Riveira, Eimi
Cendoya, Eugenia
Kuhn, Yamila Gisele
Fontana, Ariel
Ramirez, Maria Laura
Pavicich, María Agustina
Combina, Mariana
Ponsone, María Lorena
author_role author
author2 Torres Palazzolo, Carolina Andrea
Riveira, Eimi
Cendoya, Eugenia
Kuhn, Yamila Gisele
Fontana, Ariel
Ramirez, Maria Laura
Pavicich, María Agustina
Combina, Mariana
Ponsone, María Lorena
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Uvas de Mesa
Compuestos Fenólicos
Control Biológico
Levadura
Almacenamiento en Frío
Table Grapes
Phenolic Compounds
Biological Control
Yeasts
Cold Storage
Biocontrol
topic Uvas de Mesa
Compuestos Fenólicos
Control Biológico
Levadura
Almacenamiento en Frío
Table Grapes
Phenolic Compounds
Biological Control
Yeasts
Cold Storage
Biocontrol
dc.description.none.fl_txt_mv Under postharvest conditions, table grapes are exposed to multiple fungal pathogens that coexist and interact on the fruit surface. Therefore, effective biocontrol agents should exert antagonistic activity across multiple pathogens (broad-spectrum biocontrol) while operating within a complex host–microbiota–pathogen system. In this context, four psychrotrophic yeast strains, Metschnikowia pulcherrima RCM2 and ULA146, and Aureobasidium pullulans FUL14 and FUL18, were evaluated for their broad-spectrum biocontrol performance in Red Globe table grapes. Grapevine-derived extracts, rich in phenolic compounds, were included as complementary components capable of enhancing antagonistic activity. Yeasts were tested alone and in combination with extracts from canes (5% and 25%) and bunch stems (5%) on cold-stored table grapes. Treatments were evaluated under three scenarios: single-pathogen inoculation with Botrytis cinerea or Penicillium polonicum, pathogen co-inoculation with B. cinerea, P. polonicum and Alternaria alternata, and non-inoculated storage conditions. A quantitative integrative framework was applied to support the selection of broad-spectrum biocontrol treatments. Overall, the yeast strains showed biocontrol activity against all three pathogens. Yeast–extract combinations significantly reduced P. polonicum infection (p < 0.05). RCM2 exhibited the most consistent performance under pathogen co-inoculation and non-inoculated storage conditions, reaching levels comparable to sulfur dioxide (SO2) treatment. These results support the potential of psychrotrophic yeasts and grapevine by-products as eco-friendly tools for broad-spectrum postharvest disease control in table grapes. They also highlight the importance of integrative approaches that account for pathogen co-occurrence and host complexity when selecting biocontrol agents for successful technological development.
EEA Mendoza
Fil: Ferreyra, Susana G. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional Mendoza; Argentina
Fil: Torres-Palazzolo, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentina
Fil: Riveira, Eimi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional Mendoza; Argentina
Fil: Cendoya, Eugenia. Universidad Nacional de Río Cuarto. Instituto de Investigación en Micología y Micotoxicología (IMICO); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación en Micología y Micotoxicología (IMICO); Argentina
Fil: Kuhn, Yamila G. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Fontana, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Instituto de Biología Agrícola de Mendoza; Argentina
Fil: Ramirez, María Laura. Universidad Nacional de Río Cuarto. Instituto de Investigación en Micología y Micotoxicología (IMICO); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación en Micología y Micotoxicología (IMICO); Argentina
Fil: Pavicich, María Agustina. Ghent University. Faculty of Pharmaceutical Sciences. Centre of Excellence in Mycotoxicology & Public Health; Bélgica. Ghent University. Faculty of Bioscience Engineering. Laboratory of Applied Mycology and Phenomics; Bélgica
Fil: Combina, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional Mendoza; Argentina
Fil: Ponsone, María Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Regional Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
description Under postharvest conditions, table grapes are exposed to multiple fungal pathogens that coexist and interact on the fruit surface. Therefore, effective biocontrol agents should exert antagonistic activity across multiple pathogens (broad-spectrum biocontrol) while operating within a complex host–microbiota–pathogen system. In this context, four psychrotrophic yeast strains, Metschnikowia pulcherrima RCM2 and ULA146, and Aureobasidium pullulans FUL14 and FUL18, were evaluated for their broad-spectrum biocontrol performance in Red Globe table grapes. Grapevine-derived extracts, rich in phenolic compounds, were included as complementary components capable of enhancing antagonistic activity. Yeasts were tested alone and in combination with extracts from canes (5% and 25%) and bunch stems (5%) on cold-stored table grapes. Treatments were evaluated under three scenarios: single-pathogen inoculation with Botrytis cinerea or Penicillium polonicum, pathogen co-inoculation with B. cinerea, P. polonicum and Alternaria alternata, and non-inoculated storage conditions. A quantitative integrative framework was applied to support the selection of broad-spectrum biocontrol treatments. Overall, the yeast strains showed biocontrol activity against all three pathogens. Yeast–extract combinations significantly reduced P. polonicum infection (p < 0.05). RCM2 exhibited the most consistent performance under pathogen co-inoculation and non-inoculated storage conditions, reaching levels comparable to sulfur dioxide (SO2) treatment. These results support the potential of psychrotrophic yeasts and grapevine by-products as eco-friendly tools for broad-spectrum postharvest disease control in table grapes. They also highlight the importance of integrative approaches that account for pathogen co-occurrence and host complexity when selecting biocontrol agents for successful technological development.
publishDate 2026
dc.date.none.fl_str_mv 2026-07-13T12:05:38Z
2026-07-13T12:05:38Z
2026-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/26989
https://www.sciencedirect.com/science/article/abs/pii/S0168160526002928
0168-1605
1879-3460
https://doi.org/10.1016/j.ijfoodmicro.2026.111911
url http://hdl.handle.net/20.500.12123/26989
https://www.sciencedirect.com/science/article/abs/pii/S0168160526002928
https://doi.org/10.1016/j.ijfoodmicro.2026.111911
identifier_str_mv 0168-1605
1879-3460
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PE-L01-I002, Aportes para la innovación y el desarrollo en los territorios a través del fortalecimiento de la viticultura
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 Internation
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv Creative Commons Attribution-NonCommercial-ShareAlike 4.0 Internation
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv International Journal of Food Microbiology 459 : 111911. (October 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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