Heifers introduced a new subtype into a herd with persistent Staphylococcus aureus intramammary infections

Autores
Cicotello, Joaquin; Ramezanigardaloud, Nasrin; Miotti, Camila; Molineri, Ana Ines; Grunert, Tom; Suarez Archilla, Guillermo; Calvinho, Luis Fernando; Camussone, Cecilia
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study describes the spread of intramammary infections (IMI) during the first lactation of heifers that were naturally infected with Staphylococcus aureus before parturition and introduced into a herd with a high prevalence of this organism. The heifers were monitored during their first lactation to determine potential spread and persistence of IMI and to characterize the isolates that caused IMI. Milk samples were obtained from all the cows in the lactating herd at the beginning of the study and one year later. S. aureus isolated at both these sampling times were compared with those obtained from the heifers to analyse their clonal and phylogenetic relationships, employing pulse-field gel electrophoresis (PFGE), multi-locus sequence typing and Fourier transform infrared spectroscopy. Most S. aureus isolated from mammary secretions of heifers before parturition established chronic IMI during the first lactation. PFGE typing discriminated 3 clusters that were associated with origin of isolates, number of lactations and clonal complex. Differences both in the presence and expression of genes associated with virulence determinants among the major pulsotypes infecting lactating cows and those from heifers that developed persistent IMI were detected, which are indicative of distinct adaptive capacities to generate IMI.
EEA Rafaela
Fil: Cicotello, Joaquin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Cicotello, Joaquin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Ramezanigardaloud, Nasrin. University of Veterinary Medicine. Institute of Microbiology. Department of Pathobiology; Austria
Fil: Miotti, Camila. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Miotti, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Molineri, Ana Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Molineri, Ana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Grunert, Tom. University of Veterinary Medicine. Institute of Microbiology. Department of Pathobiology; Austria
Fil: Suarez Archilla, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Suarez Archilla, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Calvinho, Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Calvinho, Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Camussone, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fuente
Journal of Dairy Research 91 (4) : 420-426. (November 2024)
Materia
Novilla
Ganado Bovino
Mastítis Bovina
Enfermedades de los Animales
Heifers
Cattle
Staphylococcus aureus
Bovine Mastitis
Animal Diseases
Infecciones Intramamarias Bovinas
Bovine Intrammamary Infections
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/27016
Acceder
id INTADig_15804cb6a0040bba703bfc32bc8543ec
oai_identifier_str oai:localhost:20.500.12123/27016
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Heifers introduced a new subtype into a herd with persistent Staphylococcus aureus intramammary infectionsCicotello, JoaquinRamezanigardaloud, NasrinMiotti, CamilaMolineri, Ana InesGrunert, TomSuarez Archilla, GuillermoCalvinho, Luis FernandoCamussone, CeciliaNovillaGanado BovinoMastítis BovinaEnfermedades de los AnimalesHeifersCattleStaphylococcus aureusBovine MastitisAnimal DiseasesInfecciones Intramamarias BovinasBovine Intrammamary InfectionsThis study describes the spread of intramammary infections (IMI) during the first lactation of heifers that were naturally infected with Staphylococcus aureus before parturition and introduced into a herd with a high prevalence of this organism. The heifers were monitored during their first lactation to determine potential spread and persistence of IMI and to characterize the isolates that caused IMI. Milk samples were obtained from all the cows in the lactating herd at the beginning of the study and one year later. S. aureus isolated at both these sampling times were compared with those obtained from the heifers to analyse their clonal and phylogenetic relationships, employing pulse-field gel electrophoresis (PFGE), multi-locus sequence typing and Fourier transform infrared spectroscopy. Most S. aureus isolated from mammary secretions of heifers before parturition established chronic IMI during the first lactation. PFGE typing discriminated 3 clusters that were associated with origin of isolates, number of lactations and clonal complex. Differences both in the presence and expression of genes associated with virulence determinants among the major pulsotypes infecting lactating cows and those from heifers that developed persistent IMI were detected, which are indicative of distinct adaptive capacities to generate IMI.EEA RafaelaFil: Cicotello, Joaquin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Cicotello, Joaquin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Ramezanigardaloud, Nasrin. University of Veterinary Medicine. Institute of Microbiology. Department of Pathobiology; AustriaFil: Miotti, Camila. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Miotti, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Molineri, Ana Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Molineri, Ana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Grunert, Tom. University of Veterinary Medicine. Institute of Microbiology. Department of Pathobiology; AustriaFil: Suarez Archilla, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Suarez Archilla, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Calvinho, Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Calvinho, Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Camussone, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaCambridge University Press2026-07-15T10:32:05Z2026-07-15T10:32:05Z2025-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/27016https://www.cambridge.org/core/journals/journal-of-dairy-research/article/abs/heifers-introduced-a-new-subtype-into-a-herd-with-persistent-staphylococcus-aureus-intramammary-infections/1916703D6AA53FB91D94E9EDCFB70EE30022-02991469-7629https://doi.org/10.1017/S0022029925000202Journal of Dairy Research 91 (4) : 420-426. (November 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E5-I105-001, Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco ?Una Salud?info:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-09-24T11:43:27Zoai:localhost:20.500.12123/27016instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-09-24 11:43:28.862INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Heifers introduced a new subtype into a herd with persistent Staphylococcus aureus intramammary infections
title Heifers introduced a new subtype into a herd with persistent Staphylococcus aureus intramammary infections
spellingShingle Heifers introduced a new subtype into a herd with persistent Staphylococcus aureus intramammary infections
Cicotello, Joaquin
Novilla
Ganado Bovino
Mastítis Bovina
Enfermedades de los Animales
Heifers
Cattle
Staphylococcus aureus
Bovine Mastitis
Animal Diseases
Infecciones Intramamarias Bovinas
Bovine Intrammamary Infections
title_short Heifers introduced a new subtype into a herd with persistent Staphylococcus aureus intramammary infections
title_full Heifers introduced a new subtype into a herd with persistent Staphylococcus aureus intramammary infections
title_fullStr Heifers introduced a new subtype into a herd with persistent Staphylococcus aureus intramammary infections
title_full_unstemmed Heifers introduced a new subtype into a herd with persistent Staphylococcus aureus intramammary infections
title_sort Heifers introduced a new subtype into a herd with persistent Staphylococcus aureus intramammary infections
dc.creator.none.fl_str_mv Cicotello, Joaquin
Ramezanigardaloud, Nasrin
Miotti, Camila
Molineri, Ana Ines
Grunert, Tom
Suarez Archilla, Guillermo
Calvinho, Luis Fernando
Camussone, Cecilia
author Cicotello, Joaquin
author_facet Cicotello, Joaquin
Ramezanigardaloud, Nasrin
Miotti, Camila
Molineri, Ana Ines
Grunert, Tom
Suarez Archilla, Guillermo
Calvinho, Luis Fernando
Camussone, Cecilia
author_role author
author2 Ramezanigardaloud, Nasrin
Miotti, Camila
Molineri, Ana Ines
Grunert, Tom
Suarez Archilla, Guillermo
Calvinho, Luis Fernando
Camussone, Cecilia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Novilla
Ganado Bovino
Mastítis Bovina
Enfermedades de los Animales
Heifers
Cattle
Staphylococcus aureus
Bovine Mastitis
Animal Diseases
Infecciones Intramamarias Bovinas
Bovine Intrammamary Infections
topic Novilla
Ganado Bovino
Mastítis Bovina
Enfermedades de los Animales
Heifers
Cattle
Staphylococcus aureus
Bovine Mastitis
Animal Diseases
Infecciones Intramamarias Bovinas
Bovine Intrammamary Infections
dc.description.none.fl_txt_mv This study describes the spread of intramammary infections (IMI) during the first lactation of heifers that were naturally infected with Staphylococcus aureus before parturition and introduced into a herd with a high prevalence of this organism. The heifers were monitored during their first lactation to determine potential spread and persistence of IMI and to characterize the isolates that caused IMI. Milk samples were obtained from all the cows in the lactating herd at the beginning of the study and one year later. S. aureus isolated at both these sampling times were compared with those obtained from the heifers to analyse their clonal and phylogenetic relationships, employing pulse-field gel electrophoresis (PFGE), multi-locus sequence typing and Fourier transform infrared spectroscopy. Most S. aureus isolated from mammary secretions of heifers before parturition established chronic IMI during the first lactation. PFGE typing discriminated 3 clusters that were associated with origin of isolates, number of lactations and clonal complex. Differences both in the presence and expression of genes associated with virulence determinants among the major pulsotypes infecting lactating cows and those from heifers that developed persistent IMI were detected, which are indicative of distinct adaptive capacities to generate IMI.
EEA Rafaela
Fil: Cicotello, Joaquin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Cicotello, Joaquin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Ramezanigardaloud, Nasrin. University of Veterinary Medicine. Institute of Microbiology. Department of Pathobiology; Austria
Fil: Miotti, Camila. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Miotti, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Molineri, Ana Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Molineri, Ana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Grunert, Tom. University of Veterinary Medicine. Institute of Microbiology. Department of Pathobiology; Austria
Fil: Suarez Archilla, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Suarez Archilla, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Calvinho, Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Calvinho, Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Camussone, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
description This study describes the spread of intramammary infections (IMI) during the first lactation of heifers that were naturally infected with Staphylococcus aureus before parturition and introduced into a herd with a high prevalence of this organism. The heifers were monitored during their first lactation to determine potential spread and persistence of IMI and to characterize the isolates that caused IMI. Milk samples were obtained from all the cows in the lactating herd at the beginning of the study and one year later. S. aureus isolated at both these sampling times were compared with those obtained from the heifers to analyse their clonal and phylogenetic relationships, employing pulse-field gel electrophoresis (PFGE), multi-locus sequence typing and Fourier transform infrared spectroscopy. Most S. aureus isolated from mammary secretions of heifers before parturition established chronic IMI during the first lactation. PFGE typing discriminated 3 clusters that were associated with origin of isolates, number of lactations and clonal complex. Differences both in the presence and expression of genes associated with virulence determinants among the major pulsotypes infecting lactating cows and those from heifers that developed persistent IMI were detected, which are indicative of distinct adaptive capacities to generate IMI.
publishDate 2025
dc.date.none.fl_str_mv 2025-05
2026-07-15T10:32:05Z
2026-07-15T10:32:05Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/27016
https://www.cambridge.org/core/journals/journal-of-dairy-research/article/abs/heifers-introduced-a-new-subtype-into-a-herd-with-persistent-staphylococcus-aureus-intramammary-infections/1916703D6AA53FB91D94E9EDCFB70EE3
0022-0299
1469-7629
https://doi.org/10.1017/S0022029925000202
url http://hdl.handle.net/20.500.12123/27016
https://www.cambridge.org/core/journals/journal-of-dairy-research/article/abs/heifers-introduced-a-new-subtype-into-a-herd-with-persistent-staphylococcus-aureus-intramammary-infections/1916703D6AA53FB91D94E9EDCFB70EE3
https://doi.org/10.1017/S0022029925000202
identifier_str_mv 0022-0299
1469-7629
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001, Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco ?Una Salud?
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv Journal of Dairy Research 91 (4) : 420-426. (November 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.733355

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